Abstract:
:The kinetic mechanism of the transport catalyzed by the human glutamine/neutral amino acid transporter hASCT2 over-expressed in P. pastoris was determined in proteoliposomes by pseudo-bi-substrate kinetic analysis of the Na(+)-glutamineex/glutaminein transport reaction. A random simultaneous mechanism resulted from the experimental analysis. Purified functional hASCT2 was chemically cross-linked to a stable dimeric form. The oligomeric structure correlated well with the kinetic mechanism of transport. Half-saturation constants (Km) of the transporter for the other substrates Ala, Ser, Asn and Thr were measured both on the external and internal side. External Km were much lower than the internal ones confirming the asymmetry of the transporter. The electric nature of the transport reaction was determined imposing a negative inside membrane potential generated by K(+) gradients in the presence of valinomycin. The transport reaction resulted to be electrogenic and the electrogenicity originated from external Na(+). Internal Na(+) exerted a stimulatory effect on the transport activity which could be explained by a regulatory, not a counter-transport, effect. Native and deglycosylated hASCT2 extracted from HeLa showed the same transport features demonstrating that the glycosyl moiety has no role in transport function. Both in vitro and in vivo interactions of hASCT2 with the scaffold protein PDZK1 were revealed.
journal_name
Amino Acidsjournal_title
Amino acidsauthors
Scalise M,Pochini L,Panni S,Pingitore P,Hedfalk K,Indiveri Cdoi
10.1007/s00726-014-1808-xsubject
Has Abstractpub_date
2014-11-01 00:00:00pages
2463-75issue
11eissn
0939-4451issn
1438-2199journal_volume
46pub_type
杂志文章相关文献
AMINO ACIDS文献大全abstract::The extracellular transglutaminases (TGs) in eukaryotes are responsible for the post-translational modification of proteins through different reactions, cross-linking being the best known. In higher plants, extracellular TG appears to be involved in roles similar to those performed by the mammalian counterparties. Sin...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/s00726-013-1605-y
更新日期:2014-03-01 00:00:00
abstract::The successful prediction of protein-folding rates based on the sequence-predicted secondary structure suggests that the folding rates might be predicted from sequence alone. To pursue this question, we directly predict the folding rates from amino acid sequences, which do not require any information on secondary or t...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-011-1189-3
更新日期:2012-08-01 00:00:00
abstract::Knowing the key features of the structure and the biochemistry of proteins is crucial to improving enzymes of industrial interest like β-fructofuranosidase. Gene sacA from Bacillus licheniformis ATCC 14580 codifies a sucrose-6-phosphate hydrolase, a β-fructofuranosidase (E.C. 3.1.2.26, protein BlsacA), which has no cr...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-018-02690-2
更新日期:2019-04-01 00:00:00
abstract::The Disrupted-in-schizophrenia 1 (DISC1) gene is involved in vulnerability to neuropsychiatric disorders. Naples high-excitability (NHE) rat model neuropsychiatric problems characterized by an unbalanced mesocortical dopamine system. Here, we assessed behavioral and neurochemical effects of immunization against multim...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-014-1897-6
更新日期:2015-03-01 00:00:00
abstract::Endurance exercise training as well as leucine supplementation modulates glucose homeostasis and protein turnover in mammals. Here, we analyze whether leucine supplementation alters the effects of endurance exercise on these parameters in healthy mice. Mice were distributed into sedentary (C) and exercise (T) groups. ...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-014-1903-z
更新日期:2015-04-01 00:00:00
abstract::Treatment of developing rats with monosodium glutamate (MSG) produces an increase of glutamate levels in the brain, being this elevation dependent on both route of administration and animal's age. The capacity of exogenous MSG to induce convulsions seems to be related to the rate of glutamate elevation in the brain, r...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/BF00808094
更新日期:1991-02-01 00:00:00
abstract::The ethynylglycine synthon {(R)-2,2-dimethyl-3-(tert-butoxycarbonyl)-4-ethynyloxazolidine} is a chiral compound with valuable synthetic interest. An update on the different routes for its synthesis is reviewed and discussed. ...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/s00726-014-1902-0
更新日期:2015-02-01 00:00:00
abstract::Blood-borne angiotensin II (AngII) is known to mediate water-intake by its excitatory effect on neurons in the subfornical organ (SFO). Conversely, nitric oxide (NO) has exclusively inhibitory effects on rat SFO-neurons and on SFO-mediated water-intake. Extracellular and patch-clamp recordings from freshly dissociated...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/BF01345251
更新日期:1998-01-01 00:00:00
abstract::D-Aspartate (D-Asp) has important physiological functions, and recent studies have shown that substantial amounts of free D-Asp are present in a wide variety of mammalian tissues and cells. Biosynthesis of D-Asp has been observed in several cultured rat cell lines, and a murine gene (glutamate-oxaloacetate transaminas...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-015-1926-0
更新日期:2015-05-01 00:00:00
abstract::Cupiennin 1a, a cytolytic peptide isolated from the venom of the spider Cupiennius salei, exhibits broad membranolytic activity towards bacteria, trypanosomes, and plasmodia, as well as human blood and cancer cells. In analysing the cytolytic activity of synthesised all-D: - and all-L: -cupiennin 1a towards pro- and e...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-009-0471-0
更新日期:2011-01-01 00:00:00
abstract::Animals at the neonatal stage have to eat more to support better growth and health. However, it is difficult to understand the mechanism of feeding during an early stage of life in the brain of the rodent model. Chickens are precocial and they can look for their food by themselves right after hatching. Neonatal chicks...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/s00726-019-02762-x
更新日期:2019-08-01 00:00:00
abstract::In order to adapt to ever changing environmental conditions, bacteria sense environmental stimuli, and convert them into signals that are transduced intracellularly. Several mechanisms have evolved by which receptors transmit signals across the cytoplasmic membrane. Stimulus perception may trigger receptor dimerizatio...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/s00726-009-0235-x
更新日期:2009-09-01 00:00:00
abstract::Breast cancer susceptibility gene 2 (BRCA2) is an important tumor suppressor, which is participated in repair of damaged DNA by its highly conserved BRC repeat motifs regulating RAD51 protein homologous recombination and thereby preventing cell carcinogenesis. In this study, the BRCA2(1524-1548)-RAD51(241-260) complex...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-019-02813-3
更新日期:2020-03-01 00:00:00
abstract::Germination ofBacillus subtilis spores was initiated by L-Ala and competitively inhibited by D-Ala, suggesting the presence of an alanine receptor. The spores showed alanine racemase activity in the spore coat. To investigate the role of alanine racemase (L → D) on germination, net racemase activity was determined usi...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/BF00805804
更新日期:1993-02-01 00:00:00
abstract::The polyamines spermidine and spermine, and their precursor putrescine, have been shown to play an important role in cell migration, proliferation, and differentiation. Because of their polycationic property, polyamines are traditionally thought to be involved in DNA replication, gene expression, and protein translati...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-014-1879-8
更新日期:2015-03-01 00:00:00
abstract::Starting from a chiral 4-hydroxymethyl pyrrolidin-2-one, an isostere of α-methyl homoserine tethered on a γ-lactam ring was prepared exploiting a stereoselective acylation-methylation sequence, followed by Curtius rearrangement, and structural assignment was confirmed by n.O.e. experiments. By reverting the sequence, ...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-014-1671-9
更新日期:2014-04-01 00:00:00
abstract::Taurine, 2-aminoethylsulfonic acid, is one of the most abundant amino acids in the brain. It has various important physiological functions as a neuromodulator and antioxidant. Taurine is expected to be involved in depression; however, knowledge regarding its function in relation to depression is limited. In this study...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-012-1282-2
更新日期:2012-11-01 00:00:00
abstract::In this review, recent studies on the electrophysiological effects of de novo synthesized ("endogenous") kynurenic acid (KYNA) are discussed. Endogenous KYNA is normally formed as a byproduct of tryptophan metabolism. Evidence for a physiological role in neuronal excitability has not been strong, in part because brain...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/s007260070060
更新日期:2000-01-01 00:00:00
abstract::Until now information about the influence of puberty on gingival tissue responses to Ni-Ti alloy haven't been available. Since our previous researches have demonstrated that Ni-Ti appliances have an influence on hyperplastic gingivopathy and data has pointed out a possible hormonal influence on the susceptibility of g...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s007260200001
更新日期:2002-01-01 00:00:00
abstract::This study aimed to investigate the ability of curcumin to convert asparagine into acrylamide during heating at different temperatures. Binary and ternary model systems of asparagine-curcumin and asparagine-curcumin-fructose were used to determine the role of curcumin on acrylamide formation in competitive and uncompe...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-011-1179-5
更新日期:2013-06-01 00:00:00
abstract::In the absence of exogenous Ca(2+) and Mg(2+) and in the presence of EGTA, which favours the release of endogenous Ca(2+), the polyamine spermine is able to stimulate the activity of pyruvate dehydrogenase complex (PDC) of energized rat liver mitochondria (RLM). This stimulation exhibits a gradual concentration-depend...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-008-0099-5
更新日期:2009-03-01 00:00:00
abstract::Since the formulation of the concept of "proteomics" in 1995, a plethora of proteomic technologies have been developed in order to study proteomes of tissues, cells and organelles. The powerful new technologies enabled by proteomic approaches have lead to the application of these methods to an exponentially increasing...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/s00726-006-0460-5
更新日期:2007-09-01 00:00:00
abstract::Protooncogenes (PO) play a crucial role for brain biology and pathology. Only the concerted action of protooncogenes enables normal brain development. The reliable and sensitive quantification of brain PO is still holding centre stage in neurobiological research. The aim of our study was therefore the determination of...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/BF01318881
更新日期:1999-01-01 00:00:00
abstract::Placental vascular formation and blood flow are crucial for fetal survival, growth and development, and arginine regulates vascular development and function. This study determined the effects of dietary arginine or N-carbamylglutamate (NCG) supplementation during late gestation of sows on the microRNAs, vascular endot...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-011-0948-5
更新日期:2012-06-01 00:00:00
abstract::Properties of putrescine uptake by PotFGHI and PuuP and their physiological significance were studied using a polyamine biosynthesis and uptake deficient Escherichia coli KK3131 transformed with pACYC184 containing potFGHI or puuP. Putrescine uptake activity of E. coli KK3131 transformed with pACYC184-PotFGHI was high...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-013-1517-x
更新日期:2014-03-01 00:00:00
abstract::The hydration of selected amino acids, alanine, glycine, proline, valine, isoleucine and phenylalanine, has been studied in aqueous solutions by means of FTIR spectra of HDO isotopically diluted in H2O. The difference spectra procedure and the chemometric method have been applied to remove the contribution of bulk wat...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-015-2005-2
更新日期:2015-11-01 00:00:00
abstract::An immunocytochemical investigation of the expression of alpha(1), alpha(6), beta(2/3), gamma(2) and delta subunits was performed on rat cerebellum granule cells in culture by the two photon microscopy technique. The first four subunits appear to be expressed abundantly in these cells, whereas the delta one seems to b...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-003-0006-z
更新日期:2004-02-01 00:00:00
abstract::Oral β-alanine (βA) doses larger than 800 mg commonly result in unpleasant sensory symptoms (paresthesia). However, the association of form (pure vs. slow-release) with side-effects has not been fully described. The aim of this single-blinded, randomized three-arm clinical trial was to compare plasma kinetics and symp...
journal_title:Amino acids
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1007/s00726-011-1169-7
更新日期:2012-07-01 00:00:00
abstract::In mammals, two major routes exist for the metabolic conversion of L-glutamine to α-ketoglutarate. The most widely studied pathway involves the hydrolysis of L-glutamine to L-glutamate catalyzed by glutaminases, followed by the conversion of L-glutamate to α-ketoglutarate by the action of an L-glutamate-linked aminotr...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/s00726-015-2061-7
更新日期:2016-01-01 00:00:00
abstract::Phenolic compounds are interesting because of their antioxidant properties. In the present study, the antioxidant properties of L-tyrosine as a monophenolic and L-Dopa as a diphenolic amino acid were investigated by using different antioxidant assays: (i) 1,1-diphenyl-2-picryl-hydrazyl free radical (DPPH*) scavenging;...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-006-0379-x
更新日期:2007-01-01 00:00:00