Abstract：
:Interleukin-35 (IL-35) is a novel anti-inflammatory cytokine and has been shown to play an important role in maintaining immune homeostasis. However, the effect of IL-35 on human asthma remains unclear. The present study is to investigate the expression and significance of IL-35 in childhood asthma. Forty-one asthmatic children and forty-two healthy controls were recruited in Qilu Children's Hospital of Shandong University. Serum total immunoglobulin E level was measured by radioimmunosorbent test. Peripheral blood eosinophils were counted using BC-5800 Automatic Blood Cell Analyzer. IL-35 mRNA in peripheral blood mononuclear cells was detected by quantitative real-time polymerase chain reaction. Serum IL-35, IL-4 and interferon-γ levels were measured using enzyme-linked immunosorbent assay. The correlations among the above indexes were also analyzed using Pearson's method. Our results showed that serum total IgE, eosinophil count and serum IL-4 were significantly increased in asthmatic children compared with control children, and serum IFN-γ level in asthmatic patients was obviously lower than that in healthy controls. We also found that there was an obviously positive correlation between serum IgE and IL-4 levels in asthmatic patients. In addition, significantly negative correlation was found between serum total IgE and IFN-γ levels. More importantly, we found that the expression of IL-35 mRNA and protein was both down-regulated in asthmatic children, and serum IL-35 level was inversely related to serum IL-4 level. Moreover, significantly positive correlation was also found between serum IL-35 and IFN-γ levels. The results suggest that the decreased expression of IL-35 could be involved in the pathogenesis of childhood asthma.
journal_name
Immunol Lettjournal_title
Immunology lettersauthors
Ma Y,Liu X,Wei Z,Wang X,Xu D,Dai S,Li Y,Gao M,Ji C,Guo C,Zhang L,Wang Xdoi
10.1016/j.imlet.2014.06.002subject
Has Abstractpub_date
2014-11-01 00:00:00pages
11-7issue
1 Pt Aeissn
0165-2478issn
1879-0542pii
S0165-2478(14)00114-Xjournal_volume
162pub_type
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