Nicotine promotes late endothelial progenitor cells functional activity in a PI 3-kinase-dependent manner.

Abstract:

:Recent studies have shown that endothelial progenitor cells (EPCs) participated in angiogenic effects of nicotine and nicotine dose dependently increased the functional activity of early EPCs. The effects of nicotine on late EPCs remain to be determined. Therefore, we investigated whether nicotine had influences on the functional activity of late EPCs. Late EPCs were isolated from human umbilical cord blood and characterized. Late EPCs of 3-5 passages were treated for 32 h with either vehicle or nicotine. The proliferative, migratory, and in vitro vasculogenesis activities of late EPCs were assayed with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, modified Boyden chamber assay, and in matrigel, respectively. Late EPCs adhesion assay was performed by replating cells on fibronectin-coated dishes, and then adherent cells were counted. Nicotine enhanced proliferative, migratory, adhesive, and in vitro vasculogenesis capacities of late EPCs. These effects were significantly reduced in the presence of phosphatidylinositol (PI) 3-kinase inhibitor.

journal_name

Cell Biochem Biophys

authors

Yu M,Li Z,Shu Z,Liu Q,Sun J,Tan X

doi

10.1007/s12013-014-0013-6

subject

Has Abstract

pub_date

2014-11-01 00:00:00

pages

1023-8

issue

2

eissn

1085-9195

issn

1559-0283

journal_volume

70

pub_type

杂志文章
  • On the analysis of large-scale genomic structures.

    abstract::We apply methods from statistical physics (histograms, correlation functions, fractal dimensions, and singularity spectra) to characterize large-scale structure of the distribution of nucleotides along genomic sequences. We discuss the role of the extension of noncoding segments ("junk DNA") for the genomic organizati...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1385/CBB:42:2:145

    authors: Oiwa NN,Goldman C

    更新日期:2005-01-01 00:00:00

  • Clinical application of a dendritic cell vaccine raised against heat-shocked glioblastoma.

    abstract::Establishment of a detection platform for glioblastoma-dendritic cell (DC) vaccine preparation and to determine the efficacy of the vaccine in a clinical trial. Autologous glioblastoma-DC vaccine was prepared from a glioblast specimen procured from surgical resection. The specimen was used to enrich the vaccine with p...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-011-9265-6

    authors: Jie X,Hua L,Jiang W,Feng F,Feng G,Hua Z

    更新日期:2012-01-01 00:00:00

  • Expression and localization of estrogen receptor in human breast cancer and its clinical significance.

    abstract::The aim of this study was to analyze the clinicopathologic significance of ERα protein that localized in the cell cytoplasm and/or cell membrane of human breast cancer and explore what kind of protein that the cytoplasm/membrane ERα belongs to. ERα expressions in 61 cases of breast cancer are detected by immunohistoch...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-014-0163-6

    authors: Li L,Wang Q,Lv X,Sha L,Qin H,Wang L,Li L

    更新日期:2015-01-01 00:00:00

  • Mechanical induction of BMP-7 in osteocyte blocks glucocorticoid-induced apoptosis through PI3K/AKT/GSK3β pathway.

    abstract::Mechanical loading is known to promote osteocyte survival, whereas glucocorticoid treatment results in osteocyte apoptosis. Here, we report that BMP-7, which was secreted by osteocyte in response to mechanical loading, exerts anti-apoptotic effect against dexamethasone-induced apoptosis of osteocytes. We further show ...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-013-9543-6

    authors: Wang Z,Guo J

    更新日期:2013-11-01 00:00:00

  • Structural adaptation in adult rabbit ventricular myocytes: influence of dynamic physical interaction with fibroblasts.

    abstract::The mechanism of induction of cardiomyocyte (CM) dedifferentiation, as seen in chronic hibernating myocardium, is largely unknown. Recently, a cellular model was proposed consisting of long-term cocultures of adult rabbit CMs and cardiac fibroblasts in which typical structural characteristics of hibernation-like dedif...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1385/CBB:44:1:119

    authors: Driesen RB,Verheyen FK,Dispersyn GD,Thoné F,Lenders MH,Ramaekers FC,Borgers M

    更新日期:2006-01-01 00:00:00

  • The Use of Calcium Channel Blockers in the Treatment of Coronary Spasm and Atrioventricular Block.

    abstract::Calcium channel blockers have been used in the treatment of coronary artery spasm for many years. However, there is insufficient knowledge about their application to treat atrioventricular block caused by coronary spasm. Clinical data of five patients who were diagnosed with coronary spasm caused by atrioventricular b...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-014-0498-z

    authors: Gao B,Zhang Z,Qian J,Cao C,Hua X,Chu M,He X,Zeng H

    更新日期:2015-06-01 00:00:00

  • Assessment of Coronary Flow Reserve by Adenosine Stress Myocardial Perfusion Imaging in Patients with Hypertension.

    abstract::In this study, our aim was to assess the coronary flow reserve (CFR) by performing the adenosine stress (99m)Tc-MIBI single-photon computed tomographic (SPECT) myocardial perfusion imaging in patients with hypertension. 47 hypertensive patients with normal coronary angiography were divided into 2 groups, defined by th...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-015-0600-1

    authors: Fu Q,Zhang Q,Lu W,Wang Y,Huang Y,Wang Y,Wu Q,Lu C

    更新日期:2015-11-01 00:00:00

  • Arsenic Trioxide and Icariin Show Synergistic Anti-leukemic Activity.

    abstract::Although As2O3 (ATO) has been recommended as the front-line agent for treatment of acute promyelocytic leukemia (APL), particularly for relapsed or refractory APL, it has been associated with profound toxicity. Icariin is a natural compound with activity against a variety of cancers. This study was designed to investi...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-015-0660-2

    authors: Wang Z,Zhang H,Dai L,Song T,Li P,Liu Y,Wang L

    更新日期:2015-09-01 00:00:00

  • Chemopreventive Effects of Azadirachta indica on Cancer Marker Indices and Ultrastructural Changes During 1,2-Dimethylhydrazine-Induced Colon Carcinogenesis in Rats.

    abstract::The present study elucidated the prospective of Azadirachta indica supplementation, if any, in affording chemoprevention by modulating the altered cancer markers and ultrastructural changes in DMH-induced colorectal carcinogenesis in rats. The rats were segregated into four groups viz., normal control, DMH treated, A....

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-015-0621-9

    authors: Liu N,Sun B,Wu P,Wei X

    更新日期:2015-09-01 00:00:00

  • DNA microarray analysis for human congenital heart disease.

    abstract::Right ventricular hypertrophy and failure are prominent features in cyanotic congenital heart disease, tetralogy of Fallot (TF). Patients with TF require primary cardiac surgery at a very young age. To gain insight into the underlying molecular mechanisms of right ventricular hypertrophy and to identify gene(s) involv...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1385/CBB:44:1:001

    authors: Sharma HS,Peters TH,Moorhouse MJ,van der Spek PJ,Bogers AJ

    更新日期:2006-01-01 00:00:00

  • Calcium channel gamma subunits: a functionally diverse protein family.

    abstract::The calcium channel gamma subunits comprise an eight-member protein family that share a common topology consisting of four transmembrane domains and intracellular N- and C-termini. Although the first gamma subunit was identified as an auxiliary subunit of a voltage-dependent calcium channel, a review of phylogenetic, ...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章,评审

    doi:10.1007/s12013-007-0002-0

    authors: Chen RS,Deng TC,Garcia T,Sellers ZM,Best PM

    更新日期:2007-01-01 00:00:00

  • Experiences of microsurgical reconstruction for variant hepatic artery in living donor liver transplantation.

    abstract::There is an emergent need for improving the microsurgical technique of variant arterial anastomosis to reduce the often seen surgery-related complications. We describe in this article our experience in improving this technique, in 73 living donor liver grafts (64 right lobes, 9 left lobes) in patients with end-stage l...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-012-9421-7

    authors: Liang Y,Ye S,Shi X,Ji W,Duan W,Luo Y,Dong J

    更新日期:2013-03-01 00:00:00

  • Lipids Composition in Plant Membranes.

    abstract::The paper focuses on the selected plant lipid issues. Classification, nomenclature, and abundance of fatty acids was discussed. Then, classification, composition, role, and organization of lipids were displayed. The involvement of lipids in xantophyll cycle and glycerolipids synthesis (as the most abundant of all lipi...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章,评审

    doi:10.1007/s12013-020-00947-w

    authors: Reszczyńska E,Hanaka A

    更新日期:2020-12-01 00:00:00

  • Principles of macromolecular organization and cell function in bacteria and archaea.

    abstract::Structural organization of the cytoplasm by compartmentation is a well established fact for the eukaryotic cell. In prokaryotes, compartmentation is less obvious. Most prokaryotes do not need intracytoplasmic membranes to maintain their vital functions. This review, especially dealing with prokaryotes, will point out ...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章,评审

    doi:10.1007/BF02738242

    authors: Hoppert M,Mayer F

    更新日期:1999-01-01 00:00:00

  • Radiophotographically Controlled Nasointestinal Intubation to Treat Recurrent Postoperative Adhesive Ileus.

    abstract::This study describes clinical experience and curative effect of radiophotographically controlled nasointestinal intubation in the treatment of small intestine arrangement in recurrent postoperative adhesive ileus. A 300-cm-long nasointestinal tube was installed into the jejunum through the nasal cavity under radiophot...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-014-0496-1

    authors: Li RH,Li DC,Lv XP,Quan B

    更新日期:2015-06-01 00:00:00

  • Non-esterified fatty acids activate the AMP-activated protein kinase signaling pathway to regulate lipid metabolism in bovine hepatocytes.

    abstract::Non-esterified fatty acids (NEFAs) act as signaling molecules involved in regulating genes expression to modulate lipid metabolism. However, the regulation mechanism of NEFAs on lipid metabolism in dairy cows is unclear. The AMP-activated protein kinase (AMPK) signaling pathway plays a key role in regulating hepatic l...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-013-9629-1

    authors: Li X,Li X,Chen H,Lei L,Liu J,Guan Y,Liu Z,Zhang L,Yang W,Zhao C,Fu S,Li P,Liu G,Wang Z

    更新日期:2013-01-01 00:00:00

  • Hypothesis: Tim-3/galectin-9, a new pathway for leukemia stem cells survival by promoting expansion of myeloid-derived suppressor cells and differentiating into tumor-associated macrophages.

    abstract::Despite the improvements in chemotherapy, about 60 % of acute myeloid leukemia (AML) remission patients still relapse. Leukemic stem cells (LSCs) are the main causes for the relapse and refractory. T cell immunoglobulin mucin-3 (TIM-3), a specific surface molecule expressed on LSCs in most types of AML, is a candidate...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-014-9900-0

    authors: Gao L,Yu S,Zhang X

    更新日期:2014-09-01 00:00:00

  • Comparison Between Sporadic and Misdiagnosed Sporadic Creutzfeldt-Jakob Disease: A Report of Two Cases.

    abstract::Definite accurate diagnosis for Creutzfeldt-Jakob disease (CJD) depends on neuropathologic examination of brain biopsy or autopsy. However, transmissible nature makes the invasive examination dangerous. This study was set to determine that the clinical features are for the diagnosis of CJD through a comparison study. ...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-014-0458-7

    authors: Zhao X,Yu Y,Zhao Z,Xu J

    更新日期:2015-06-01 00:00:00

  • Estimation of Some Oxidative Stress Parameters and Blood Pressure After Administration of Endothelin-1 (ET-1) in Rats.

    abstract::The aim of the study was to investigate changes in the plasma antioxidative activity and in lipid peroxidation after administration of endothelin-1 (ET-1) and endothelin receptor blockers and additionally, to estimate blood pressure. The study was performed on male Wistar rats (n = 6 per group) divided into 4 groups w...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-014-0371-0

    authors: Kleniewska P,Kowalczyk A,Ciesla W,Goraca A

    更新日期:2015-04-01 00:00:00

  • Preserved coupling of oxidative phosphorylation but decreased mitochondrial respiratory capacity in IL-1beta-treated human peritoneal mesothelial cells.

    abstract::The peritoneal mesothelium acts as a regulator of serosal responses to injury, infection, and neoplastic diseases. After inflammation of the serosal surfaces, proinflammatory cytokines induce an "activated" mesothelial cell phenotype, the mitochondrial aspect of which has not previously been studied. After incubation ...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1385/CBB:44:2:179

    authors: Stadlmann S,Renner K,Pollheimer J,Moser PL,Zeimet AG,Offner FA,Gnaiger E

    更新日期:2006-01-01 00:00:00

  • Interfacial Electrostatic Properties of Hydrated Mesoporous and Nanostructured Alumina Powders by Spin Labeling EPR.

    abstract::Acid-base equilibria and interfacial electrostatic properties of hydrated mesoporous and nanostructured alumina powders are determining factors for the use of these materials in heterogeneous catalysis and as a sorption media for filtration and chromatographic applications including life sciences. Here spin probe elec...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-016-0767-0

    authors: Kovaleva EG,Molochnikov LS,Stepanova DP,Pestov AV,Trofimov DG,Kirilyuk IA,Smirnov AI

    更新日期:2017-06-01 00:00:00

  • Coating and selective deposition of nanofilm on silicone rubber for cell adhesion and growth.

    abstract::A recently developed method for surface modification, layer-by-layer (LbL) assembly, has been applied to silicone, and its ability to encourage endothelial cell growth and control cell growth patterns has been examined. The surfaces studied consisted of a precursor, with alternating cationic polyethyleneimine (PEI) an...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1385/CBB:38:2:103

    authors: Ai H,Lvov YM,Mills DK,Jennings M,Alexander JS,Jones SA

    更新日期:2003-01-01 00:00:00

  • Single channel recordings from synaptosomal AMPA receptors.

    abstract::Synaptic glutamate receptors play a prominent role in the excitatory neurotransmission in the vertebrate central nervous system. Although elucidation of the functional properties of glutamate receptors using electrophysiologic analyses has yielded important information, methodological and technological limitations hav...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1385/CBB:42:1:075

    authors: Vaithianathan T,Manivannan K,Kleene R,Bahr BA,Dey MP,Dityatev A,Suppiramaniam V

    更新日期:2005-01-01 00:00:00

  • Sema4D knockdown in oligodendrocytes promotes functional recovery after spinal cord injury.

    abstract::Semaphorin4D (Sema4D) belongs to Semaphorins family and is secreted and membrane-bound protein. Its function on angiogenesis and axon regeneration makes it an ideal therapeutic target for spinal cord injury (SCI). Here we examined Sema4D expression profile by real-time PCR and western blot and found Sema4D was upregul...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-013-9727-0

    authors: Zhang HL,Wang J,Tang L

    更新日期:2014-04-01 00:00:00

  • Development of vascular tissue and stress inducible hybrid-synthetic promoters through dof-1 motifs rearrangement.

    abstract::A Caulimovirus-based hybrid-promoter, EFCFS, was derived by fusing the distal region (-227 to -54, FUAS) of Figwort mosaic virus full-length transcript promoter (F20) with the core promoter (-151 to +12, FS3CP) domain of Figwort mosaic virus sub-genomic transcript promoter (FS3). The hybrid-promoter (EFCFS) showed enh...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-012-9359-9

    authors: Ranjan R,Dey N

    更新日期:2012-07-01 00:00:00

  • Influence of magnetic field on brain activity during administration of caffeine.

    abstract::The aim of the present work is to evaluate the effect of caffeine, the world's most popular psychoactive drug, on the electric activity of the rat's brain that exposed to extremely low-frequency magnetic field (ELF-MF), during 15 days. The obtained results showed that administration of caffeine in a group of rats by d...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-013-9584-x

    authors: El Gohary MI,Salama AA,El Saeid AA,El Sayed TM,Kotb HSh

    更新日期:2013-01-01 00:00:00

  • Resveratrol induces pro-oxidant effects and time-dependent resistance to cytotoxicity in activated hepatic stellate cells.

    abstract::Resveratrol (RSV) is known for its antioxidant properties; however, this compound has been proposed to have cytotoxic and pro-oxidant effects depending on its concentration and time of exposure. We previously reported the cell cycle arrest effect of low doses of RSV in GRX cells, an activated hepatic stellate cell mod...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-013-9703-8

    authors: Martins LA,Coelho BP,Behr G,Pettenuzzo LF,Souza IC,Moreira JC,Borojevic R,Gottfried C,Guma FC

    更新日期:2014-03-01 00:00:00

  • Introducing a New Model of Sweet Taste Receptor, a Class C G-protein Coupled Receptor (C GPCR).

    abstract::The structure of sweet taste receptor (STR), a heterodimer of class C G-protein coupled receptors comprising T1R2 and T1R3 molecules, is still undetermined. In this study, a new enhanced model of the receptor is introduced based on the most recent templates. The improvement, stability, and reliability of the model are...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-019-00872-7

    authors: Kashani-Amin E,Sakhteman A,Larijani B,Ebrahim-Habibi A

    更新日期:2019-09-01 00:00:00

  • Association with leptin gene C.-2548 G>A polymorphism, serum leptin levels, and body mass index in Turkish obese patients.

    abstract::Leptin is a protein hormone which plays a critical role in the regulation of both body-weight through reducing food intake and stimulating energy expenditure. Several polymorphisms in leptin gene (LEP), which encodes for leptin, have been described. However, its association with obesity is still controversial. Therefo...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-012-9427-1

    authors: Sahin DS,Tumer C,Demir C,Celik MM,Celik M,Ucar E,Gunesacar R

    更新日期:2013-03-01 00:00:00

  • Synthetic miR-145 Mimic Enhances the Cytotoxic Effect of the Antiangiogenic Drug Sunitinib in Glioblastoma.

    abstract::Although aggressive therapeutic regimen has been applied in the treatment of Glioblastoma (GBM), the prognosis of patients with GBM remains poor. Preclinical studies have demonstrated the efficacy of Suntinib in GBM both in vitro and in vivo. In this study, we showed that the cytotoxicity was enhanced by transfection ...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1007/s12013-014-0501-8

    authors: Liu H,Liu Z,Jiang B,Huo L,Liu J,Lu J

    更新日期:2015-06-01 00:00:00