Abstract:
:A colorimetric microadhesion assay that allows the quantitative determination of carcinoembryonic antigen (CEA)-mediated homophilic cell adhesion to CEA immobilized on 96-well polyvinyl chloride plates is described. Chinese hamster ovary (CHO) cells transfected with a full-length CEA cDNA were used as indicator cells. After dislodging nonadherent cells, specifically bound cells were quantified by a colorimetric analysis based on the ability of live cells to reduce the dye 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) to a blue formazan product. The domains of CEA responsible for the homophilic cell adhesion were analyzed by inhibition assays using anti-CEA monoclonal antibodies whose reactive domains were already known. The involvement of domain N and possibly subdomain A3 of CEA in the homophilic cell adhesion has been suggested.
journal_name
Immunol Investjournal_title
Immunological investigationsauthors
Kuwahara M,Kuroki M,Haruno M,Murakami M,Arakawa F,Oikawa S,Nakazato H,Matsuoka Ydoi
10.3109/08820139409066832subject
Has Abstractpub_date
1994-11-01 00:00:00pages
367-80issue
6-7eissn
0882-0139issn
1532-4311journal_volume
23pub_type
杂志文章abstract::Studies presented examine the origin of IgA plasma cells in B lineage chimeric mice constructed by reconstituting lethally irradiated mice with a mixture of syngeneic bone marrow cells and peritoneal cells from Ig heavy chain allotype congenic donors. In these mice, essentially all B cells in spleen and Peyer's patche...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820138909112226
更新日期:1989-01-01 00:00:00
abstract::Dengue viruses (DV) infection is an important public health issue all over the world. Although the pathogenesis remains unclear, the overwhelmingly triggered immune responses have been consistently observed. Recently, we and other researchers demonstrated that the natural hosts for DV are dendritic cells (DC), the pri...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1081/imm-200038680
更新日期:2004-01-01 00:00:00
abstract::Recently, we reported the preparation and antigen-presenting properties of hybridoma B-cell clones obtained after fusing non-secreting, non-antigen presenting Balb/c 653-myeloma cells with non-immune SJL spleen cells. It was found that antigen presentation at the clonal level can be specific or non-specific, depending...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820138909045785
更新日期:1989-10-01 00:00:00
abstract::Food allergy is an important and common health issue, and there is a need to identify and characterize the sensitizing mechanisms. One of the common causes of food allergy is ovalbumin (OVA), a dietary antigen from eggs. We hypothesized that OVA-induced food allergy in the gut involves the activation of the chemokine ...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1081/imm-120027682
更新日期:2004-02-01 00:00:00
abstract::The central dogma of gene therapy relies on the application of novel therapeutic genes to treat or prevent diseases. The main types of vectors used for gene transfer are adenovirus, retrovirus, lentivirus, liposome, and adeno-associated virus vectors. Gene therapy has emerged as a promising alternative for the treatme...
journal_title:Immunological investigations
pub_type: 杂志文章,评审
doi:10.3109/08820139.2016.1168831
更新日期:2016-07-01 00:00:00
abstract::Dendritic cells (DCs) are potent antigen-presenting cells that can be matured in vitro from immature dendritic cells (iDCs) in the presence of several biological agents such as cytokine cocktail, CD40L, TNF-a and antigen loading, which are necessary and achieved using various protocols, such as lipofection, passive pu...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820139.2012.757234
更新日期:2013-01-01 00:00:00
abstract::The complement system which is a critical mediator of innate immunity plays diverse roles in the neuropathogenesis of HIV-1 infection such as clearing HIV-1 and promoting productive HIV-1 replication. In the development of HIV-1 associated neurological disorders (HAND), there may be an imbalance between complement act...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820139.2017.1371891
更新日期:2017-11-01 00:00:00
abstract::Identification and characterization of antigenic proteins of Setaria cervi (bovine filarial parasite) adults and microfilariae was done by immunoblotting technique using hyperimmune rabbit sera against S. cervi and Brugia malayi. The antigens recognized by these sera were detected by using 125I protein-A followed by a...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820138709030571
更新日期:1987-04-01 00:00:00
abstract::Seven commercially available intravenous immunoglobulins (IVIG) preparations, a gamma globulin prepared by ethanol fractionation, and an experimental IgG isolated by a chromatographic procedure were compared in several tests. Split products were present in preparations manufactured by procedures involving protease tre...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820138809055724
更新日期:1988-04-01 00:00:00
abstract::In this study, we aimed to explore the association among gene variants of five cytokines, tumor necrosis factor alpha (TNF-α), transforming growth factor beta-1 (TGF-β1), interferon gamma (IFN-γ), interleukin-6 (IL-6), and interleukin-10 (IL-10), and clinical parameters and prognosis in patients with multiple myeloma ...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820139.2016.1208219
更新日期:2017-01-01 00:00:00
abstract:BACKGROUND:Loquat leaf is an herb that is commonly used in traditional Chinese medicine (TCM) for its anti-inflammatory properties. Numerous studies have demonstrated that Th17 cells play a fundamental role in mediating SLE pathological deterioration. In our study, we investigated the inhibitory effect of pentacyclic t...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820139.2019.1698599
更新日期:2020-08-01 00:00:00
abstract::We examined the blocking ability of poly(vinyl alcohol) (PVA) in enzyme immunoassays by coating polystyrene microtiter wells with PVA of different molecular weights (MW) and percent hydrolysis (%Hyd). Blocking ability was measured by the differences in non-specific binding of an anti-rabbit IgG-horseradish peroxidase ...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820139409066836
更新日期:1994-11-01 00:00:00
abstract::Prolonged survival of C57BL/6 (B6) mice bearing syngenic EL-4 leukemia cells resulted from immunization with irradiated EL-4 cells on the day of inoculation of live leukemia cells. No prolongation of survival was observed if the irradiated cells were injected 6 or 13 days after live cell inoculation. Protection also w...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820138509022676
更新日期:1985-12-01 00:00:00
abstract::A previous study has demonstrated that both interferon-gamma (IFN-gamma) and lipopolysaccharide (LPS) were needed to induce the production of nitric oxide (NO) in BNL CL.2 cells, murine embryonic liver cells. We here demonstrate that when BNL CL.2 cells were cultured with serum-free medium, they were induced to produc...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820139909061144
更新日期:1999-03-01 00:00:00
abstract::Immunological parameters previously shown to constitute an increased risk for progression towards AIDS have been observed in clinically asymptomatic individuals considered to be at risk for this syndrome. These parameters include severely decreased numbers of T helper cells (count below 400/mm3 blood for CD4+ cells we...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820138809055726
更新日期:1988-04-01 00:00:00
abstract::Implantation of pellets containing 75 mg of morphine induced short term (4 day) morphine dependence and markedly reduced total number of spleen cells of BALB/c mice, without affecting total body or liver weight. Polyclonal responses induced by anti-CD3 antibodies, Concanavalin A or Escherichia coli lipopolysaccharide ...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820139509066864
更新日期:1995-05-01 00:00:00
abstract::A well-characterized recombinant anti-idiotype to an anti-DNA antibody can be useful for studies of the regulation of anti-DNA-producing B cells. Using a hybridoma technique, a monoclonal anti-idiotypic antibody, designated O2F3, was obtained, and its scFv gene was constructed. O2F3 single chain Fv (scFv) was produced...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1081/imm-120016241
更新日期:2002-08-01 00:00:00
abstract:BACKGROUND:Mouse experimental autoimmune encephalomyelitis (EAE) is widely used model of multiple sclerosis (MS). The role of autoreactive CD4+ and CD8+ T cells in the development of mouse EAE has been demonstrated. However, little information is available about the relation between the frequency and reactivity of myel...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820139.2017.1367008
更新日期:2018-01-01 00:00:00
abstract::Jeevaneeya rasayana is an ayurvedic polyherbal formulation, with antirheumatic potential. The present study investigates the therapeutic efficacy of isolated total alkaloid fraction of Jeevaneeya Rasayana (AJR) in treating rheumatoid arthritis in a rat model of Adjuvant-induced arthritis (AIA). Paw swelling, inflammat...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820139.2014.936937
更新日期:2015-01-01 00:00:00
abstract::In the last 10 years many of the superantigens of the microbial world have been defined and the mechanisms of cellular interaction between lymphocytes and antigen presenting cells has been elucidated in great detail. The consequences of superantigen stimulation of the immune system, though less well defined, can be co...
journal_title:Immunological investigations
pub_type: 杂志文章,评审
doi:10.3109/08820139709048933
更新日期:1997-01-01 00:00:00
abstract::Rotaviruses were first recognized about 15 years ago in association with diarrhea in children and animals. Since then, rotaviruses have been determined to be the most important viral agent that causes clinically significant diarrhea in children and a need for an effective vaccination program has been recognized. This ...
journal_title:Immunological investigations
pub_type: 杂志文章,评审
doi:10.3109/08820138909112264
更新日期:1989-01-01 00:00:00
abstract::Genome-wide association studies have led to the discovery of several susceptibility genes related to autoimmune thyroid diseases (AITDs). However, controversial results have been reported regarding the role of single-nucleotide polymorphism (SNP) of CD40 in the disease susceptibility. The objective of this study was t...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820139.2017.1319382
更新日期:2017-08-01 00:00:00
abstract::IL-3-like activity (IL-3-LA) is a growth factor that stimulates stem cell maturation. We examined the production of IL-3-LA by peripheral blood mononuclear cells of 10 patients with relapsing-remitting multiple sclerosis (MS) following one year of treatment with intravenous immunoglobulins (IVIG). The results were com...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820139509060704
更新日期:1995-08-01 00:00:00
abstract::Mannan binding lectin (MBL) deficiency has been associated with increased susceptibility to vaginitis in humans due to Candida albicans. In these studies we assessed the utility of recombinant human MBL (rhMBL) as a therapeutic against experimental C. albicans vaginitis. After intravenous treatment of uninfected mice ...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820139.2011.569627
更新日期:2011-01-01 00:00:00
abstract:BACKGROUND:Grass pollen is a major cause of allergy throughout the world. The only treatment targeting the causes and not only the symptoms of allergy is specific immunotherapy (IT). A number of controlled trials demonstrated the efficacy of IT in grass pollen allergic subjects, most using extracts of multiple grasses ...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820131003796876
更新日期:2010-01-01 00:00:00
abstract:PURPOSE:To assess cellular composition and local cytokine response in vitreous humor of tubercular uveitis. METHODS:Cells were collected from vitreous cassettes and peripheral blood of 8 tubercular uveitis and 5 control subjects, undergoing vitrectomy and analyzed by flow cytometry for cellular composition, activation...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820139.2018.1470640
更新日期:2018-08-01 00:00:00
abstract::Thrombocytopenia as a complicating event in human immunodeficiency infection is a rather common hematological disorder and in many aspects resembles idiopathic(immune) thrombocytopenic purpura (ITP). Consequently, treatment of severe cases is similar to treating ITP, but steroids should be administered with particular...
journal_title:Immunological investigations
pub_type: 杂志文章,评审
doi:10.3109/08820139109050791
更新日期:1991-04-01 00:00:00
abstract::The induction of immune tolerance is the most common consequence of protein feeding, i.e., "oral tolerance". In this study we investigated the genetic basis of oral tolerance using various kinds of recombinant and congenic mice, and the cells involved in the development of this phenomenon in mice. The footpad swelling...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820139309063423
更新日期:1993-08-01 00:00:00
abstract::Regulatory T cells (Tregs) play pivotal roles in limiting the duration and magnitude of immune response against infectious agents and self-antigens. This is accomplished through contact-dependent and -independent mechanisms that involve crosstalk between Treg cells and other immune and tissue-specific cell types. The ...
journal_title:Immunological investigations
pub_type: 杂志文章,评审
doi:10.1080/08820139.2016.1197242
更新日期:2016-11-01 00:00:00
abstract::Porphyromonas gingivalis (P.g) is the primary bacterial agent in many forms of chronic periodontitis. Since polymorphonuclear leukocytes (PMNs) are first-line responders to P.g.- induced inflammation, and fibrinogen is important for in vivo PMN in this disease, we have studied the effect of N-formyl-methionyl-leucyl-p...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820130500496811
更新日期:2006-01-01 00:00:00