Abstract:
PURPOSE:To assess cellular composition and local cytokine response in vitreous humor of tubercular uveitis. METHODS:Cells were collected from vitreous cassettes and peripheral blood of 8 tubercular uveitis and 5 control subjects, undergoing vitrectomy and analyzed by flow cytometry for cellular composition, activation status, proinflammatory cytokine expression, and uptake of TLR9 ligand, CpG ODN 2216. RESULTS:CD3 + T cells with equal proportion of CD4+ and CD8 + T cells formed major fraction of infiltrating cells. The vitreous humor showed higher expression of recent activation marker, CD69, and proinflammatory cytokines, IFN-γ and IL-17A, in CD4 + T cells as compared to peripheral blood. Lastly, intraocular CD4 + T cells showed reduced uptake of ODN 2216 than peripheral blood. CONCLUSIONS:Our results indicate that local antigenic stimuli trigger T cell infiltration and activation of CD4 + T cells that are hyporesponsive to TLR9 stimulation. These infiltrating T cells might be responsible in further aggravating ocular inflammation.
journal_name
Immunol Investjournal_title
Immunological investigationsauthors
Sharma RK M.Sc,Gupta V M.S,Bansal R M.S,Sharma K M.D,Gupta A M.S,Sachdeva N Ph.Ddoi
10.1080/08820139.2018.1470640subject
Has Abstractpub_date
2018-08-01 00:00:00pages
615-631issue
6eissn
0882-0139issn
1532-4311journal_volume
47pub_type
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journal_title:Immunological investigations
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