Immune Profiling of T Cells Infiltrating Vitreous Humor in Tubercular Uveitis.

Abstract:

PURPOSE:To assess cellular composition and local cytokine response in vitreous humor of tubercular uveitis. METHODS:Cells were collected from vitreous cassettes and peripheral blood of 8 tubercular uveitis and 5 control subjects, undergoing vitrectomy and analyzed by flow cytometry for cellular composition, activation status, proinflammatory cytokine expression, and uptake of TLR9 ligand, CpG ODN 2216. RESULTS:CD3 + T cells with equal proportion of CD4+ and CD8 + T cells formed major fraction of infiltrating cells. The vitreous humor showed higher expression of recent activation marker, CD69, and proinflammatory cytokines, IFN-γ and IL-17A, in CD4 + T cells as compared to peripheral blood. Lastly, intraocular CD4 + T cells showed reduced uptake of ODN 2216 than peripheral blood. CONCLUSIONS:Our results indicate that local antigenic stimuli trigger T cell infiltration and activation of CD4 + T cells that are hyporesponsive to TLR9 stimulation. These infiltrating T cells might be responsible in further aggravating ocular inflammation.

journal_name

Immunol Invest

authors

Sharma RK M.Sc,Gupta V M.S,Bansal R M.S,Sharma K M.D,Gupta A M.S,Sachdeva N Ph.D

doi

10.1080/08820139.2018.1470640

subject

Has Abstract

pub_date

2018-08-01 00:00:00

pages

615-631

issue

6

eissn

0882-0139

issn

1532-4311

journal_volume

47

pub_type

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