当前位置： SCI文献检索 > GYNECOLOGIC ONCOLOGY期刊下所有文献 > Establishment and characterization of a cell line (CUMC-3) derived from a human squamous carcinoma of the uterine cervix.

Establishment and characterization of a cell line (CUMC-3) derived from a human squamous carcinoma of the uterine cervix.

Abstract：

:A new cell line, CUMC-3, has been derived from an invasive nonkeratinizing squamous cell carcinoma of the uterine cervix in a 32-year-old patient. It has been maintained in long-term culture for 59 months, and passaged over 310 times. Monolayer-cultured cells were polygonal in shape, showing a pavement-like arrangement and a tendency to pile up without contact inhibition. The epithelial nature of the cultured CUMC-3 cells was also confirmed by transmission electron microscopy which demonstrated the presence of desmosomes and tonofilaments. The cells were highly tumorigenic in nude mice and the transplanted tumors were poorly differentiated squamous carcinoma which closely resembled the original tumor. Cultured cells obtained from the CUMC-3-derived nude mouse tumor, CUMC-3N, also were studied for its characterization. Repeated chromosome analysis revealed a stable clone with the modal chromosome number of 78. The metaphase of this cell line had multiple structural aberrations of chromosomes 1, 3, 8, 10, 11, 20, and X and showed several markers of unknown origin. The results of isozyme analyses were distinct from the HeLa cell line. The identical genetic signature was demonstrated both in CUMC-3 and in CUMC-3N cells. Cultured CUMC-3 cells produced human chorionic gonadotropin beta-subunit and tumor antigen of squamous cell carcinoma (TA-4). Cytosol estrogen receptors were found in this cell line but progesterone receptors were not measured. HLA typing of CUMC-3 cells indicated the presence of DR4, DR8, DQw3, and DQw6. The result of oncogene analysis using Southern blotting technique revealed no amplification of oncogene c-myc. Analysis of the DNA samples extracted from the CUMC-3 cells showed the presence of human papillomavirus type 16 DNA. Using the single-strand conformation polymorphism technique, we have screened CUMC-3 cells for p53 mutation in exons 4 to 9. No mobility shift was observed in this cell line. This cell line may be useful in studying the in vitro and in vivo properties of human cervical carcinoma.

journal_name

Gynecol Oncol

journal_title

Gynecologic oncology

authors

Kim JW,Cho YH,Lyu MS,Lee CG,Jung JK,Kim SJ,Namkoong SE

doi

10.1006/gyno.1995.1098

subject

Has Abstract

pub_date

1995-04-01 00:00:00

pages

47-60

issue

1

eissn

0090-8258

issn

1095-6859

pii

S0090-8258(85)71098-0

journal_volume

57

pub_type

杂志文章

相关文献

GYNECOLOGIC ONCOLOGY文献大全
  • Patient characteristics and opioid use prior to discharge after open gynecologic surgery in an enhanced recovery after surgery (ERAS) program.

    abstract：OBJECTIVE:To identify clinical and demographic characteristics associated with the absence of opioid usage on the day before discharge among patients undergoing open gynecologic surgery within an enhanced recovery after surgery (ERAS) program. METHODS:This was a single institution retrospective cohort study including ...

    journal_title：Gynecologic oncology

    pub_type： 杂志文章

    doi：10.1016/j.ygyno.2019.03.101

    authors： Hillman RT,Sanchez-Migallon A,Meyer LA,Iniesta MD,Cain KE,Siverand AM,Veum SPL,Suki TS,Lasala JD,Ramirez PT

    更新日期：2019-06-01 00:00:00

  • Management of uterine adenosarcomas with and without sarcomatous overgrowth.

    abstract：OBJECTIVES:Uterine adenosarcomas (AS) are rare tumors composed of malignant stromal and benign epithelial components. We sought to evaluate the role of primary surgery, adjuvant treatments, and salvage therapies for patients with uterine adenosarcomas. METHODS:We identified all patients diagnosed with AS from 1990 to ...

    journal_title：Gynecologic oncology

    pub_type： 杂志文章

    doi：10.1016/j.ygyno.2012.12.036

    authors： Tanner EJ,Toussaint T,Leitao MM Jr,Hensley ML,Soslow RA,Gardner GJ,Jewell EL

    更新日期：2013-04-01 00:00:00

  • Does neoadjuvant chemotherapy impair long-term survival for ovarian cancer patients? A nationwide Danish study.

    abstract：OBJECTIVE:In Denmark, the proportion of women with ovarian cancer treated with neoadjuvant chemotherapy (NACT) has increased, and the use of NACT varies among center hospitals. We aimed to evaluate the impact of first-line treatment on surgical outcome and median overall survival (MOS). METHODS:All patients treated in...

    journal_title：Gynecologic oncology

    pub_type： 杂志文章

    doi：10.1016/j.ygyno.2013.11.035

    authors： Fagö-Olsen CL,Ottesen B,Kehlet H,Antonsen SL,Christensen IJ,Markauskas A,Mosgaard BJ,Ottosen C,Soegaard CH,Soegaard-Andersen E,Hoegdall C

    更新日期：2014-02-01 00:00:00

  • The impact of novel retinoids in combination with platinum chemotherapy on ovarian cancer stem cells.

    abstract：OBJECTIVE:Retinoids are important modulators of cell growth, differentiation, and proliferation. 9cUAB30, 9cUAB124, and 9cUAB130 are three novel retinoid compounds that show cytotoxic effects in other malignancies. We evaluated these novel retinoids in combination with chemotherapy against ovarian cancer stem cells (CS...

    journal_title：Gynecologic oncology

    pub_type： 杂志文章

    doi：10.1016/j.ygyno.2011.12.425

    authors： Whitworth JM,Londoño-Joshi AI,Sellers JC,Oliver PJ,Muccio DD,Atigadda VR,Straughn JM Jr,Buchsbaum DJ

    更新日期：2012-04-01 00:00:00

  • Endometrial cancer in morbidly obese women: do racial disparities affect surgical or survival outcomes?

    abstract：OBJECTIVE:Endometrial cancer mortality disproportionately affects black women and whether greater prevalence of obesity plays a role in this disparity is unknown. We examine the effect of race on post-surgical complications, length of stay, and mortality specifically in a morbidly obese population. METHODS:Black and w...

    journal_title：Gynecologic oncology

    pub_type： 杂志文章

    doi：10.1016/j.ygyno.2014.01.013

    authors： Cote ML,Ruterbusch JJ,Ahmed Q,Bandyopadhyay S,Alosh B,Abdulfatah E,Seward S,Morris R,Ali-Fehmi R

    更新日期：2014-04-01 00:00:00

  • Prognostic implications of histological tumor regression (Böhm's score) in patients receiving neoadjuvant chemotherapy for high grade serous tubal & ovarian carcinoma.

    abstract：OBJECTIVE:Primary objective of this study was to determine prognostic significance of Bohm's histopathological regression score in patients who received neoadjuvant chemotherapy (NACT) for treatment of high grade serous (HGS) tubal & ovarian carcinoma. METHODS:This was a retrospective cohort study of patients who rece...

    journal_title：Gynecologic oncology

    pub_type： 杂志文章

    doi：10.1016/j.ygyno.2018.08.042

    authors： Rajkumar S,Polson A,Nath R,Lane G,Sayasneh A,Jakes A,Begum S,Mehra G

    更新日期：2018-11-01 00:00:00

  • Efficacy of intraperitoneal cisplatin as consolidation therapy in patients with pathologic complete remission following front-line therapy for epithelial ovarian cancer. Consolidative intraperitoneal cisplatin in ovarian cancer.

    abstract：OBJECTIVE:The aim of this study is to evaluate the efficacy of intraperitoneal cisplatin as consolidation treatment in epithelian ovarian cancer patients with complete pathologic response following front-line platin-based chemotherapy. PATIENTS AND METHODS:Thirty patients who had no evidence of disease as assessed by ...

    journal_title：Gynecologic oncology

    pub_type： 临床试验,杂志文章

    doi：10.1016/j.ygyno.2003.10.002

    authors： Topuz E,Eralp Y,Saglam S,Saip P,Aydiner A,Berkman S,Yavuz E

    更新日期：2004-01-01 00:00:00

  • Mesorectal lymph node involvement and prognostic implications at total pelvic exenteration for gynecologic malignancies.

    abstract：OBJECTIVES:To determine the incidence and prognostic implications of positive mesorectal lymph nodes in patients undergoing total pelvic exenteration for recurrent gynecologic malignancies. METHODS:We performed a retrospective chart review of all patients who had undergone total pelvic exenteration for a gynecologic m...

    journal_title：Gynecologic oncology

    pub_type： 杂志文章

    doi：10.1016/j.ygyno.2005.08.039

    authors： Mourton SM,Chi DS,Sonoda Y,Alektiar KM,Venkatraman ES,Barakat RR,Abu-Rustum NR

    更新日期：2006-03-01 00:00:00

  • A case of successful pregnancy after treatment of invasive cervical cancer with systemic chemotherapy and conization.

    abstract：BACKGROUND:The standard therapy for invasive uterine cervical cancer causes loss of the woman's fertility. We report a successful pregnancy in a patient who desired fertility-sparing management of invasive cervical cancer and was treated with systemic chemotherapy and conization. CASE:A 28-year-old nulliparous woman w...

    journal_title：Gynecologic oncology

    pub_type： 杂志文章

    doi：10.1016/j.ygyno.2005.08.018

    authors： Kobayashi Y,Akiyama F,Hasumi K

    更新日期：2006-01-01 00:00:00

  • Contemporary management of ovarian germ cell tumors and remaining controversies.

    abstract：:Ovarian germ cell tumors (GCTs) are rare in adults, but are more common in adolescents and young adults. Contemporary management of ovarian GCTs is evolving as collaboration among pediatric, medical, and gynecologic oncologists increases, and studies increasingly incorporate female adult patients. Despite an improved ...

    journal_title：Gynecologic oncology

    pub_type： 杂志文章,评审

    doi：10.1016/j.ygyno.2020.05.007

    authors： Veneris JT,Mahajan P,Frazier AL

    更新日期：2020-08-01 00:00:00

  • Preoperative hysteroscopic assessment of cervical invasion by endometrial carcinoma: a retrospective study.

    abstract：OBJECTIVE:The aim of this study was to evaluate the efficacy of hysteroscopy, using normal saline (NS) or carbon dioxide (CO2) as the distention medium, in assessing tumor invasion of the uterine cervix by endometrial carcinoma. METHODS:A retrospective study was conducted in 200 consecutive patients with endometrial c...

    journal_title：Gynecologic oncology

    pub_type： 杂志文章

    doi：10.1006/gyno.2001.6270

    authors： Lo KW,Cheung TH,Yim SF,Chung TK

    更新日期：2001-08-01 00:00:00

  • Vulvovaginal melanoma: report of seven cases and literature review.

    abstract：:Five cases of primary vaginal melanoma were treated at UCLA Medical Center between 1976 and 1986. Two additional cases of melanoma arising at the junction of the vulva and vagina are presented. One of seven (13%) patients is alive, with a median time to recurrence of 7 months, and median survival of 31 months. Four of...

    journal_title：Gynecologic oncology

    pub_type： 杂志文章,评审

    doi：10.1016/0090-8258(89)90603-3

    authors： Brand E,Fu YS,Lagasse LD,Berek JS

    更新日期：1989-04-01 00:00:00

  • A phase I clinical trial of Ad5/3-Δ24, a novel serotype-chimeric, infectivity-enhanced, conditionally-replicative adenovirus (CRAd), in patients with recurrent ovarian cancer.

    abstract：OBJECTIVE:The conditionally replicative adenovirus Ad5/3-Δ24 has a type-3 knob incorporated into the type-5 fiber that facilitates enhanced ovarian cancer infectivity. Preclinical studies have shown that Ad5/3-Δ24 achieves significant oncolysis and anti-tumor activity in ovarian cancer models. The purpose of this study...

    journal_title：Gynecologic oncology

    pub_type： 杂志文章

    doi：10.1016/j.ygyno.2013.06.003

    authors： Kim KH,Dmitriev IP,Saddekni S,Kashentseva EA,Harris RD,Aurigemma R,Bae S,Singh KP,Siegal GP,Curiel DT,Alvarez RD

    更新日期：2013-09-01 00:00:00

  • A population-based case-control study of genetic variation in cytokine genes associated with risk of cervical and vulvar cancers.

    abstract：OBJECTIVE:Persistent infection with oncogenic human papillomavirus (HPV) is known to be the necessary cause of cervical cancer and a majority of vulvar cancers. Persistent HPV infections must evade host immune responses, including cytokines released by activated T-helper (Th) cells. In this study, we investigated the r...

    journal_title：Gynecologic oncology

    pub_type： 杂志文章

    doi：10.1016/j.ygyno.2015.07.110

    authors： Hardikar S,Johnson LG,Malkki M,Petersdorf EW,Galloway DA,Schwartz SM,Madeleine MM

    更新日期：2015-10-01 00:00:00

  • Phase II study of neoadjuvant chemotherapy with mitomycin-c, vincristine and cisplatin (MVC) in patients with stages IB2-IIB cervical carcinoma.

    abstract：OBJECTIVE:The efficacy and toxicity of neoadjuvant chemotherapy (NAC) with mitomycin-C, vincristine and cisplatin (MVC) were assessed in bulky cervical carcinoma patients. METHODS:Forty-six patients with stage IB2 to IIB cervical cancer were treated with intravenous combination of mitomycin-C 10 mg/m(2), vincristine 1...

    journal_title：Gynecologic oncology

    pub_type： 杂志文章

    doi：10.1016/j.ygyno.2006.07.006

    authors： Choi CH,Kim TJ,Lee JW,Kim BG,Lee JH,Bae DS

    更新日期：2007-01-01 00:00:00

  • Radical hysterectomy for cervical cancer: morbidity and survival in relation to weight and age.

    abstract：:The records of 123 patients with Stage I cervical cancer who underwent radical hysterectomy with pelvic lymphadenectomy and para-aortic node sampling from 1981 to 1988 were reviewed to assess the risks of surgery associated with increasing weight and age. Fifty-four patients were obese (20% or more over ideal body wei...

    journal_title：Gynecologic oncology

    pub_type： 杂志文章

    doi：10.1016/0090-8258(92)90312-7

    authors： Levrant SG,Fruchter RG,Maiman M

    更新日期：1992-06-01 00:00:00

  • An immunohistochemical study of colon-ovarian tumor antigen and colon-specific antigen in gynecologic tumors.

    abstract：:The distribution of colon--ovarian tumor antigen (COTA) and colon-specific antigen (CSA) was studied immunohistochemically in gynecologic tumors. The antigens were absent in the serous benign tumors of the ovary and in the normal ovarian tissues, whereas they were detected in some of the mucinous benign tumors. COTA a...

    journal_title：Gynecologic oncology

    pub_type： 杂志文章

    doi：10.1016/0090-8258(89)90019-x

    authors： Ueda G,Shimizu C,Saito J,Tanaka Y,Inoue M,Tanizawa O

    更新日期：1989-10-01 00:00:00

  • Close relationship between removal of circumflex iliac nodes to distal external iliac nodes and postoperative lower-extremity lymphedema in uterine corpus malignant tumors.

    abstract：OBJECTIVE:The aim of this study was to confirm a causal relationship between removal of circumflex iliac nodes to the distal external iliac nodes (CINDEIN) and lower-extremity lymphedema after systematic lymphadenectomy in patients with uterine corpus malignancies. METHODS:A retrospective chart review was carried out ...

    journal_title：Gynecologic oncology

    pub_type： 杂志文章

    doi：10.1016/j.ygyno.2015.07.003

    authors： Todo Y,Yamazaki H,Takeshita S,Ohba Y,Sudo S,Minobe S,Okamoto K,Kato H

    更新日期：2015-10-01 00:00:00

  • Analysis of vitamin D receptor expression and clinical correlations in patients with ovarian cancer.

    abstract：OBJECTIVE:Although the antiproliferative and differentiating properties of vitamin D have been demonstrated, its effects on cancer cells are variable. Little is known about vitamin D receptor (VDR) levels in patients with ovarian cancer. In this population we sought to determine correlations between VDR expression, cli...

    journal_title：Gynecologic oncology

    pub_type： 杂志文章

    doi：10.1016/j.ygyno.2010.06.008

    authors： Silvagno F,Poma CB,Realmuto C,Ravarino N,Ramella A,Santoro N,D'Amelio P,Fuso L,Pescarmona G,Zola P

    更新日期：2010-10-01 00:00:00

  • Measuring cause-and-effect relationships without randomized clinical trials: Quasi-experimental methods for gynecologic oncology research.

    abstract：:Clinical research in gynecologic oncology has seen a proliferation of studies that investigate the effectiveness of treatments using existing data sources such as cancer registries, electronic health records, and insurance claims. These observational studies are often feasible when randomized trial may not be, and may...

    journal_title：Gynecologic oncology

    pub_type： 杂志文章,评审

    doi：10.1016/j.ygyno.2018.11.006

    authors： Moss HA,Melamed A,Wright JD

    更新日期：2019-03-01 00:00:00

  • Baboon cervical colposcopy, histology, and cytology.

    abstract：:Colposcopic cervical/vaginal examinations were performed on 10 healthy adult baboons. Five of the ten baboons had a "satisfactory" colposcopic cervical examination with columnar epithelium visible on the exocervix. However, only three of five with "satisfactory" colposcopic exams had large visible transformation zones...

    journal_title：Gynecologic oncology

    pub_type： 杂志文章

    doi：10.1016/0090-8258(84)90216-6

    authors： Micha JP,Quimby F

    更新日期：1984-03-01 00:00:00

  • Women with HIV are more commonly infected with non-16 and -18 high-risk HPV types.

    abstract：OBJECTIVE:To review and summarize evidence from clinical, translational and epidemiologic studies which have examined the clinically relevant aspects of HPV type prevalence and cervical dysplasia in HIV-infected women. METHODS:Relevant studies were identified through a MEDLINE search. References of identified reports ...

    journal_title：Gynecologic oncology

    pub_type： 杂志文章,评审

    doi：10.1016/j.ygyno.2009.10.058

    authors： McKenzie ND,Kobetz EN,Hnatyszyn J,Twiggs LB,Lucci JA 3rd

    更新日期：2010-03-01 00:00:00

  • Sorafenib in combination with weekly topotecan in recurrent ovarian cancer, a phase I/II study of the Hoosier Oncology Group.

    abstract：OBJECTIVE:This trial determined the efficacy and tolerability of sorafenib and weekly topotecan in patients with platinum-resistant ovarian cancer (OC) or primary peritoneal carcinomatosis (PPC). METHODS:Primary endpoints were maximum tolerated dose of sorafenib with weekly topotecan (phase I) and response rate (phase...

    journal_title：Gynecologic oncology

    pub_type： 杂志文章,多中心研究

    doi：10.1016/j.ygyno.2011.08.033

    authors： Ramasubbaiah R,Perkins SM,Schilder J,Whalen C,Johnson CS,Callahan M,Jones T,Sutton G,Matei D

    更新日期：2011-12-01 00:00:00

  • Enhancement of sensitivity to cisplatin by orobol is associated with increased mitochondrial cytochrome c release in human ovarian carcinoma cells.

    abstract：OBJECTIVES:Based on our previous report showing that orobol, a potent phosphatidylinositol 4-kinase (PI4K) inhibitor, produced cisplatin (DDP) sensitivity, we have determined the mechanism of orobol-sensitization effect. METHODS AND RESULTS:Orobol produced >2-fold DDP sensitivity in human ovarian carcinoma 2008 cells ...

    journal_title：Gynecologic oncology

    pub_type： 杂志文章

    doi：10.1016/s0090-8258(03)00316-0

    authors： Isonishi S,Saitou M,Yasuda M,Ochiai K,Tanaka T

    更新日期：2003-08-01 00:00:00

  • High risk malignant gestational trophoblastic disease: experience with triple chemotherapy (MAC) in Third World circumstances.

    abstract：:Fifty-seven patients with malignant gestational trophoblastic disease (GTD) were treated by the Gynecologic Oncology Unit of the Tygerberg Hospital, Parowvallei, RSA, between 1977 and 1986. Treatment was primarily with triple chemotherapy (MAC) followed by citrovorum factor. The total remission rate in the 23 patients...

    journal_title：Gynecologic oncology

    pub_type： 杂志文章

    doi：10.1016/0090-8258(89)90630-6

    authors： du Toit JP

    更新日期：1989-03-01 00:00:00

  • Preoperative radiation therapy in clinical stage II endometrial carcinoma.

    abstract：:From 1980 to 1987, 30 patients with FIGO clinical Stage II carcinoma of the endometrium were treated with 5000 cGy preoperative pelvic radiation therapy at Thomas Jefferson University Hospital. Patients with gross cervical disease received additional intracavitary irradiation with a tandem and ovoids for a combined to...

    journal_title：Gynecologic oncology

    pub_type： 临床试验,杂志文章

    doi：10.1016/0090-8258(92)90281-m

    authors： Reisinger SA,Staros EB,Feld R,Mohiuddin M,Lewis GC

    更新日期：1992-05-01 00:00:00

  • Elevated nm23 protein expression is correlated with diminished progression-free survival in patients with epithelial ovarian carcinoma.

    abstract：OBJECTIVES:The role of the candidate metastasis-suppressor gene nm23-H1 first characterized in breast cancer remains controversial, with both metastasis suppression and disease progression being linked to elevated nm23-H1 gene expression in different human tumor types. We sought to characterize (1) the pattern and inte...

    journal_title：Gynecologic oncology

    pub_type： 杂志文章

    doi：10.1006/gyno.1996.0056

    authors： Srivatsa PJ,Cliby WA,Keeney GL,Dodson MK,Suman VJ,Roche PC,Podratz KC

    更新日期：1996-03-01 00:00:00

  • Death from anaphylaxis to cisplatin: a case report.

    abstract：:A patient with a history of ovarian carcinoma that had responded well to cisplatin was treated with platinum agents for a recurrence. She had a seizure while receiving carboplatin and was switched to cisplatin. The patient was premedicated with antihistamines and steroids. After approximately 400 micrograms of cisplat...

    journal_title：Gynecologic oncology

    pub_type： 杂志文章

    doi：10.1006/gyno.1994.1098

    authors： Zweizig S,Roman LD,Muderspach LI

    更新日期：1994-04-01 00:00:00

  • The results of treatment of epithelial ovarian cancer after centralisation of primary surgery. Results from North Jutland, Denmark.

    abstract：OBJECTIVE:The study was performed to evaluate the results of treatment of ovarian carcinoma after the introduction of centralised primary surgery in the County of North Jutland, Denmark. METHOD:Prospective study of consecutive cases of ovarian cancer undergoing primary surgical treatment at the Gynecologic Oncologic C...

    journal_title：Gynecologic oncology

    pub_type： 杂志文章

    doi：10.1016/j.ygyno.2005.07.121

    authors： Soegaard Andersen E,Knudsen A,Svarrer T,Lund B,Nielsen K,Grove A,Tetsche M

    更新日期：2005-12-01 00:00:00

  • Human papilloma virus, vulvar dystrophy, and vulvar carcinoma: differential expression of human papillomavirus and vulvar dystrophy in the presence and absence of squamous cell carcinoma of the vulva.

    abstract：:In situ hybridization (ISH) and polymerase chain reaction (PCR) for several common HPV types were performed on 41 cases of vulvar dystrophy, 19 of which were associated with previous or simultaneous invasive squamous cell carcinoma of the vulva. Three of the 19 cases (16%) of dystrophy associated with cancer were foun...

    journal_title：Gynecologic oncology

    pub_type： 杂志文章

    doi：10.1006/gyno.1996.0151

    authors： Sagerman PM,Choi YJ,Hu Y,Niedt GW

    更新日期：1996-06-01 00:00:00