Abstract：
OBJECTIVE:Retinoids are important modulators of cell growth, differentiation, and proliferation. 9cUAB30, 9cUAB124, and 9cUAB130 are three novel retinoid compounds that show cytotoxic effects in other malignancies. We evaluated these novel retinoids in combination with chemotherapy against ovarian cancer stem cells (CSCs) in vitro and in an ex vivo model. METHODS:A2780 cells were plated in 96-well plates and treated with retinoid, carboplatin, or combination therapy. Cell viability was evaluated using ATPLite assay. The A2780 cell line was also analyzed for CSCs by evaluating ALDH activity using flow cytometry. A2780 cells treated ex vivo with retinoids and chemotherapy were injected into the flank of athymic nude mice in order to evaluate subsequent tumor initiating capacity. RESULTS:A2780 cells were sensitive to treatment with retinoids and carboplatin. The best treatment resulted from the combination of retinoid 9cUAB130 and carboplatin. Untreated A2780 cells demonstrated ALDH activity in 3.3% of the cell population. Carboplatin treatment enriched ALDH activity to 27.3%, while 9cUAB130±carboplatin maintained the ALDH positive levels similar to untreated controls (2.3% and 6.7%, respectively). Similar results were found in tumorsphere-forming conditions. Flank injections of ex vivo treated A2780 cells resulted in 4/4 mice developing tumors at 40 days in the untreated group, while 0/4 tumors developed in the 9cUAB130 and carboplatin treated group. CONCLUSION:Combination treatment with carboplatin and retinoids reduced cell-viability, reduced CSC marker expression, and inhibited tumorigenicity, making it a more effective treatment when compared with carboplatin alone.
journal_name
Gynecol Oncoljournal_title
Gynecologic oncologyauthors
Whitworth JM,Londoño-Joshi AI,Sellers JC,Oliver PJ,Muccio DD,Atigadda VR,Straughn JM Jr,Buchsbaum DJdoi
10.1016/j.ygyno.2011.12.425subject
Has Abstractpub_date
2012-04-01 00:00:00pages
226-30issue
1eissn
0090-8258issn
1095-6859pii
S0090-8258(11)01398-9journal_volume
125pub_type
杂志文章abstract：OBJECTIVE:The objective of this study was to determine whether there is a link between integrins and components of the plasminogen system, regarding tumor cell invasion to the artificial extracellular matrix. METHODS:Immunocytochemistry was performed with antibodies directed against integrins alpha2beta1, alpha4beta1,...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1006/gyno.2001.6410
更新日期：2002-01-01 00:00:00
abstract：:The steroid receptor content of the primary endometrial cancer of 22 patients who were treated for recurrent or advanced disease has been measured and correlated with response to medroxyprogesterone acetate. No patient with a progesterone receptor (PR)-negative tumor responded and only 2 patients with PR-positive tumo...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/0090-8258(85)90269-0
更新日期：1985-07-01 00:00:00
abstract：INTRODUCTION:The ability to minimize residual disease during primary cytoreductive surgery is the strongest predictor for improved overall survival in advanced ovarian cancer. But while the probability to achieve a macroscopic complete resection increases if surgery is preceded by neoadjuvant chemotherapy (NACT), survi...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2019.02.019
更新日期：2019-05-01 00:00:00
abstract：OBJECTIVE:The present study aimed to evaluate the association between altered methylation and histologically confirmed high grade cervical intraepithelial neoplasia (hgCIN). METHODS:Methylation levels in selected host (CADM1, MAL, DAPK1) and HPV (L1_I, L1_II, L2) genes were measured by pyrosequencing in DNA samples ob...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2018.08.031
更新日期：2018-11-01 00:00:00
abstract：OBJECTIVE:The aim of this study was to estimate the efficacy of an intensive CDP program, as well as to identify the predictors associated with lymphedema severity and response to CDP in lower limb lymphedema (LLL) after pelvic cancer therapy. METHODS:We performed a retrospective review of post-pelvic cancer LLL patie...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2012.03.017
更新日期：2012-06-01 00:00:00
abstract：:Human papillomavirus (HPV) has been implicated in the development of cervical cancer. Newly developed molecular techniques have facilitated HPV identification and typing of cervical lesions. Several studies have suggested a strong correlation between HPV16 and intraepithelial cervical neoplasia. However, there is no a...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/0090-8258(92)90191-k
更新日期：1992-07-01 00:00:00
abstract：OBJECTIVE:To compare the surgical and histological outcomes of diaphragmatic peritonectomy vs. full thickness resection with pleurectomy during Visceral-Peritoneal Debulking. METHODS:Service evaluation protocol (Trust number 3265). All patients with stage IIIC-IV ovarian cancer who had diaphragmatic surgery between Ap...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2015.12.004
更新日期：2016-03-01 00:00:00
abstract：:Normal and neoplastic endometrial tissues were examined immunohistochemically for chromogranin which was shown to be a specific marker for neuroendocrine cells. All 20 normal endometriums and 20 endometrial carcinomas without argyrophilia were chromogranin negative. Endometrial carcinomas with argyrophilia tested were...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/0090-8258(87)90288-5
更新日期：1987-06-01 00:00:00
abstract：:Forty-five patients with invasive cervical squamous cell carcinoma were registered in the Tom Baker Cancer Centre from 1980 to 1985. The natural history of 22 patients (48.9%) who developed persistent or recurrent disease including 11 Stage IB, 8 Stage IIB, and 3 Stage IIIB is reported. Only one patient who had the un...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/0090-8258(88)90020-0
更新日期：1988-06-01 00:00:00
abstract：:Endometrial adenocarcinoma in young women below the age of 45 years and in a functioning endometrium is rare. We present the case of a 41-year-old woman, who without clinical risk factors, was found to have a focal well-differentiated endometrial adenocarcinoma in the background of a histologically secretory endometri...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1006/gyno.1993.1122
更新日期：1993-05-01 00:00:00
abstract：OBJECTIVE:Members of the glutathione S-transferase (GST) family have been shown to have functional polymorphisms that may affect drug metabolism and influence the effects of chemotherapy and survival from cancer. GSTM1, GSTT1, and GSTP1 genotypes were evaluated for their role in ovarian cancer treatment and survival. ...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2005.08.035
更新日期：2006-02-01 00:00:00
abstract：OBJECTIVE:Approximately 2 million women worldwide are infected with high-risk human papillomaviruses (HPV), resulting in a substantial risk for the development of invasive lower genital malignancies. This study was undertaken to determine the effects of vaccination with a protein encoding a bacterial heat shock protein...
journal_title：Gynecologic oncology
pub_type： 杂志文章,多中心研究
doi：10.1016/j.ygyno.2007.05.038
更新日期：2007-09-01 00:00:00
abstract：OBJECTIVE:To investigate the effect of interleukin-7 (IL-7) gene transfection into an established ovarian carcinoma cell line (SKOV3) in vitro and evaluate the tumorigenicity of SKOV3-IL-7 in severe combined immunodeficient (SCID) mice. METHODS:IL-7 gene was transfected into SKOV3 cells by liposome. IL-7 mRNA and prot...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2003.10.036
更新日期：2004-02-01 00:00:00
abstract：:Many malignancies appear to occur with increased frequency and aggressive patterns of spread in patients seropositive for human immunodeficiency virus (HIV). The relationship between HIV infection and cervical neoplasia suggests that these patients present with more advanced disease and demonstrate poor response to th...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1006/gyno.1993.1224
更新日期：1993-09-01 00:00:00
abstract：OBJECTIVE:To identify patients with endometrial cancer at risk for hematogenous, lymphatic, or peritoneal recurrence (or combinations of them) who might potentially benefit from target-based therapies. METHODS:During a 13-year period, 915 patients had endometrial cancer managed with hysterectomy and standard adjuvant ...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2004.06.042
更新日期：2004-10-01 00:00:00
abstract：:Vaginal evisceration is a rare event. This case report describes a 45-year-old woman who presented 8 days following radical hysterectomy and lymph node dissection for stage 1B squamous cell carcinoma of the cervix with small bowel evisceration through the vagina. She was treated by laparotomy and resection anastamosis...
journal_title：Gynecologic oncology
pub_type： 杂志文章,评审
doi：10.1006/gyno.1998.5294
更新日期：1999-04-01 00:00:00
abstract：:Invasive carcinomas of the uterine cervix of 38 patients were examined for the presence of human papillomavirus (HPV) genomes and for the state of the c-myc and Ha-ras oncogenes. A combination of Southern blot hybridization and polymerase chain reaction revealed the presence of the genome of HPV type 16 in 17 tumors (...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/0090-8258(92)90220-d
更新日期：1992-09-01 00:00:00
abstract：OBJECTIVE:The origins and clinical significance of endosalpingiosis (ES), ectopic tubal epithelium, are not well understood. These investigations aim to characterize ES as it relates to normal fallopian tube, ovarian surface and serous neoplasms. METHODS:A retrospective review of pathology reports from all prophylacti...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2013.12.007
更新日期：2014-02-01 00:00:00
abstract：OBJECTIVE:To examine associations between post-diagnosis use of antihypertensive (AH) medications including thiazide diuretics (TDs), angiotensin converting enzyme inhibitors (ACEIs), beta blockers (BBs) [both non-selective (NSBBs) and selective (SBBs)] and calcium channel blockers (CCBs) and ovarian cancer-specific su...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2019.05.030
更新日期：2019-08-01 00:00:00
abstract：OBJECTIVE:To analyze the expression of Heparanase, an enzyme involved in cancer metastasis and angiogenesis, in ovarian and breast carcinoma cells in effusions. METHODS:Heparanase protein expression was analyzed in malignant effusions from ovarian (=200) and breast (=41) carcinoma patients using immunocytochemistry. T...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2006.08.045
更新日期：2007-02-01 00:00:00
abstract：:Abnormal nuclear DNA content, as determined by flow cytometry, when combined with conventional prognostic variables such as tumor grade or stage at diagnosis, appears to identify patients who are at increased risk for recurrence of disease. The DNA content of ovarian dysgerminoma, a tumor that is homologous to testicu...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/0090-8258(92)90004-3
更新日期：1992-01-01 00:00:00
abstract：BACKGROUND:An epigenetic approach to explaining endometrial carcinogenesis necessitates good understanding of Ras association domain family 1 isoform A (RASSF1A) promoter methylation data from primary studies. AIMS:Differential magnitude of reported associations between RASSF1A promoter methylation and endometrial can...
journal_title：Gynecologic oncology
pub_type： 杂志文章,meta分析,评审
doi：10.1016/j.ygyno.2017.06.017
更新日期：2017-09-01 00:00:00
abstract：:In a study of 73 patients, diagnosed with recurrent squamous cell carcinoma of the vulva between 1975 and 1990, the effect of clinical variables on the outcome was evaluated. The overall 5-year survival rate was 35.2%. Of the 73 patients, 33 (45.2%) originally had Stage I or II disease and 40 (54.8%) Stage III or IVA;...
journal_title：Gynecologic oncology
pub_type： 杂志文章,评审
doi：10.1006/gyno.1993.1032
更新日期：1993-02-01 00:00:00
abstract：BACKGROUND:Primary non-gestational choriocarcinoma of the female genital tract has been described in the ovaries and is very unusual in other genital sites. CASE:Primary non-gestational uterine cervical choriocarcinoma was diagnosed in a patient, 32, single, without previous sexual contact nor antecedent pregnancy, ad...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2005.03.045
更新日期：2005-07-01 00:00:00
abstract：OBJECTIVE:To investigate the STATs signaling pathway activated by VEGF in human hemopoietic progenitor cells. METHODS:CD34(+) hemopoietic progenitor cells, which isolated from umbilical cord blood, were treated with VEGF or culture supernatant of ovarian carcinoma cell line which could secrete large amount of VEGF, ph...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2004.03.038
更新日期：2004-07-01 00:00:00
abstract：OBJECTIVES:Routine lymphadenectomy (LA) in early stage endometrial cancer does not improve survival. However, in the absence of lymph node metastasis, radiotherapy (RT) could be withheld and hence could result in less morbidity. Our aim was to evaluate health related quality of life (HRQL) in endometrial cancer survivo...
journal_title：Gynecologic oncology
pub_type： 杂志文章,多中心研究,随机对照试验
doi：10.1016/j.ygyno.2012.06.007
更新日期：2012-10-01 00:00:00
abstract：OBJECTIVE:To examine patterns of patient travel among women with ovarian cancer and to explore the association between travel distance and short and long-term outcomes. METHODS:Women with stage II-IV epithelial ovarian cancer diagnosed from 2004 to 2016 who underwent primary surgery were identified in the National Can...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2020.05.017
更新日期：2020-08-01 00:00:00
abstract：:Malignant ovarian germ cell tumors (OGCT) and sex cord-stromal tumors (OSCST), each of which account for less than 5% of all ovarian malignancies, are much less common than epithelial ovarian cancer. In young patients suspected of having an OGCT, laparotomy is initially indicated for both diagnosis and treatment. For ...
journal_title：Gynecologic oncology
pub_type： 杂志文章,评审
doi：
更新日期：1994-12-01 00:00:00
abstract：OBJECTIVE:Epithelial ovarian cancer (EOC) spreads intra-abdominally and to the retroperitoneal lymph nodes. A greater number of distant metastases are revealed by (18)F-fluorodeoxyglucose (FDG) positron emission tomography/computed tomography (PET/CT) compared to conventional imaging methods. We aimed to investigate th...
journal_title：Gynecologic oncology
pub_type： 临床试验,杂志文章
doi：10.1016/j.ygyno.2012.04.023
更新日期：2012-07-01 00:00:00
abstract：PURPOSE:There are extremely limited data available in the general oncology or gynecologic cancer literature to document the effectiveness of antiemetic therapy over multiple courses of cytotoxic chemotherapy. METHODS:To examine this highly clinically relevant issue, we analyzed the complete treatment course of patient...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1006/gyno.2002.6656
更新日期：2002-06-01 00:00:00
