Vascular endothelial growth factor (VEGF) and ovarian carcinoma cell supernatant activate signal transducers and activators of transcription (STATs) via VEGF receptor-2 (KDR) in human hemopoietic progenitor cells.

Abstract:

OBJECTIVE:To investigate the STATs signaling pathway activated by VEGF in human hemopoietic progenitor cells. METHODS:CD34(+) hemopoietic progenitor cells, which isolated from umbilical cord blood, were treated with VEGF or culture supernatant of ovarian carcinoma cell line which could secrete large amount of VEGF, phosphorylation and nuclear translocation of STAT3 and STAT5 were then detected by Western Blot and immunocytochemistry. Expression of VEGFR2/KDR on CD34(+) cells was studied by immunocytochemistry. The specific VEGFR2/KDR heptapeptide antagonist ATWLPPR was used to identify whether the activation of STATs signaling pathway was specifically mediated by VEGFR2/KDR. RESULTS:The concentration of VEGF in SKOV3-supernatant was 4024.84+/- 505.59 pg/ml. CD34(+) progenitor cells could express VEGFR2/KDR. When CD34(+) cells were stimulated by VEGF and SKOV3-supernatant, STAT3 appeared tyrosine-phosphorylation and nuclear translocation, but STAT5 was only phosphorylated, and not translocated. When ATWLPPR was used to block the binding of VEGF to KDR, VEGF and the SKOV3-supernatant failed to activate the phosphorylation of STAT3 and STAT5. CONCLUSIONS:STAT3 may participate in the signal transduction pathways activated by VEGF specifically mediated by VEGFR2/KDR in human hemopoietic progenitor cells, and the aforementioned signaling pathway participated in the interaction of ovarian carcinoma cells and progenitor cells.

journal_name

Gynecol Oncol

journal_title

Gynecologic oncology

authors

Ye F,Chen HZ,Xie X,Ye DF,Lu WG,Ding ZM

doi

10.1016/j.ygyno.2004.03.038

subject

Has Abstract

pub_date

2004-07-01 00:00:00

pages

125-33

issue

1

eissn

0090-8258

issn

1095-6859

pii

S0090825804001957

journal_volume

94

pub_type

杂志文章
  • CO2 laser therapy for cervical intraepithelial neoplasia.

    abstract::One hundred and fifty-six patients with cervical intraepithelial neoplasia (CIN) were treated with CO2 laser and followed for 2 1/2 to 6 years. There were 19 recurrences, of which 14 occurred in patients treated with a power density of less than 1000 W/cm2 to a depth of 6 mm. All of the recurrences were subjected to a...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章

    doi:10.1016/0090-8258(85)90006-x

    authors: Caglar H,Ayhan A,Hreshchyshyn MM

    更新日期:1985-09-01 00:00:00

  • Synchronous renal cell carcinoma and gynecologic malignancies.

    abstract:BACKGROUND:Synchronicity of renal cell carcinoma and gynecologic malignancies is a rare condition and standardized treatment does not exist. CASES:Three cases of synchronous renal cell carcinoma and gynecologic malignancies are described. All three cases underwent optimal cytoreductive surgery for the gynecologic mali...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章

    doi:10.1006/gyno.2002.6856

    authors: Wong C,Plante M,Eltabbakh G

    更新日期:2003-01-01 00:00:00

  • Prognostic significance of growth characteristics of xenotransplanted ovarian carcinomas into nude mice.

    abstract::Human ovarian carcinomas (n = 215) were xenotransplanted into thymus-aplastic female nu/nu mice (NMRI). Growth of transplants was defined as take-off rate, growth rapidity, and feasibility of passages. In 204 cases these growth characteristics could be correlated with survival time of the individual tumor-donor patien...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章

    doi:10.1016/0090-8258(86)90066-1

    authors: Kleine W

    更新日期:1986-09-01 00:00:00

  • A phase II study of Hsp-7 (SGN-00101) in women with high-grade cervical intraepithelial neoplasia.

    abstract:OBJECTIVE:Approximately 2 million women worldwide are infected with high-risk human papillomaviruses (HPV), resulting in a substantial risk for the development of invasive lower genital malignancies. This study was undertaken to determine the effects of vaccination with a protein encoding a bacterial heat shock protein...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章,多中心研究

    doi:10.1016/j.ygyno.2007.05.038

    authors: Roman LD,Wilczynski S,Muderspach LI,Burnett AF,O'Meara A,Brinkman JA,Kast WM,Facio G,Felix JC,Aldana M,Weber JS

    更新日期:2007-09-01 00:00:00

  • The role of cancer antigen 125 (CA 125) in the management of ovarian epithelial carcinomas.

    abstract::From June 1, 1984, to May 31, 1985, 98 cases of epithelial ovarian carcinomas were assessed and followed prospectively using a new murine monoclonal antibody OC 125 which detects the antigen CA 125. Serous tumors comprised 43.7% of cases, mucinous tumors 20.4%, endometrioid tumors 16%, and other epithelial tumors 19.4...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章

    doi:10.1016/0090-8258(88)90042-x

    authors: Altaras MM,Goldberg GL,Levin W,Bloch B,Darge L,Smith JA

    更新日期:1988-05-01 00:00:00

  • Diagnosis of endometrial cancer in patients with postmenopausal bleeding by analysis of the lactate dehydrogenase isoenzyme activity profile in uterine fluid.

    abstract:OBJECTIVES:We have previously shown that high activity of lactate dehydrogenase (LD) isoenzymes 4 and 5 in terine aspirates is a marker for endometrial carcinoma. The purpose of this study was to identify an abnormal activity profile of LD2-5 using LD1 as an internal standard, thereby being able to dispense with measur...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章

    doi:10.1016/j.ygyno.2004.02.014

    authors: Niklasson O,Skude G,Marsal K,Casslén B

    更新日期:2004-05-01 00:00:00

  • Gestational trophoblastic diseases: 1. Pathophysiology of hyperglycosylated hCG.

    abstract:OBJECTIVE:Hyperglycosylated hCG (hCG-H) is a glycosylation variant of hCG produced by cytotrophoblast cells at implantation of pregnancy and in choriocarcinoma. We investigated the biological function of hCG-H in invasion in vitro and in vivo and the use of hCG-H antibodies in blocking tumorigenesis and cancer growth i...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章

    doi:10.1016/j.ygyno.2005.12.047

    authors: Cole LA,Dai D,Butler SA,Leslie KK,Kohorn EI

    更新日期:2006-08-01 00:00:00

  • Gene expression profiling of in vitro radiation resistance in cervical carcinoma: a feasibility study.

    abstract:OBJECTIVE(S):To determine the feasibility of integrating an in vitro chemo-radiation response assay (IVRRA) with a gene microarray system to investigate the molecular patterns of expression that contribute to radiation resistance in cervical cancer. METHODS:Viable primary untreated cervical cancer specimens were obtai...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章

    doi:10.1016/j.ygyno.2005.05.043

    authors: Tewari D,Monk BJ,Al-Ghazi MS,Parker R,Heck JD,Burger RA,Fruehauf JP

    更新日期:2005-10-01 00:00:00

  • Transitional cell carcinoma of the endometrium.

    abstract::Only one case of endometrial transitional cell carcinoma (TCC) has been previously reported in the literature. We report a second case. In both the previously reported case and in this case there was a separate focus of TCC in the adnexa. Both patients were treated with adjuvant whole pelvic radiation and are alive an...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章

    doi:10.1006/gyno.1996.0047

    authors: Spiegel GW,Austin RM,Gelven PL

    更新日期:1996-02-01 00:00:00

  • A preoperative risk-scoring system to predict lymph node metastasis in endometrial cancer and stratify patients for lymphadenectomy.

    abstract:OBJECTIVE:This study aimed to validate the preoperative scoring system adopted in the Kanagawa Cancer Center (KCC) to stratify endometrial cancer patients for lymphadenectomy according to the risk of developing lymph node metastasis (LNM). METHODS:The records of 432 and 221 uterine cancer patients treated in the KCC a...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章

    doi:10.1016/j.ygyno.2016.06.004

    authors: Imai K,Kato H,Katayama K,Nakanishi K,Kawano A,Iura A,Konnai K,Onose R,Hirahara F,Miyagi E

    更新日期:2016-08-01 00:00:00

  • Glutathione S-transferase polymorphisms and ovarian cancer treatment and survival.

    abstract:OBJECTIVE:Members of the glutathione S-transferase (GST) family have been shown to have functional polymorphisms that may affect drug metabolism and influence the effects of chemotherapy and survival from cancer. GSTM1, GSTT1, and GSTP1 genotypes were evaluated for their role in ovarian cancer treatment and survival. ...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章

    doi:10.1016/j.ygyno.2005.08.035

    authors: Beeghly A,Katsaros D,Chen H,Fracchioli S,Zhang Y,Massobrio M,Risch H,Jones B,Yu H

    更新日期:2006-02-01 00:00:00

  • Role of an early second-look laparotomy in ovarian cancer.

    abstract::Of a group of 68 patients treated with standard polychemotherapy (CAP-5), 52 were evaluated by an early second-look laparotomy, preferably after three cycles of treatment. Of 21 patients with initial tumor residuals smaller than 2 cm, only 5 had residual tumor, and of 31 patients with tumor larger than 2 cm, 27 had re...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章

    doi:10.1016/0090-8258(89)90055-3

    authors: van Lith JM,Bouma J,Aalders JG,Boonstra H,Sleijfer DT,Willemse PH

    更新日期:1989-11-01 00:00:00

  • Spontaneous internal jugular vein thrombosis associated with leiomyosarcoma of the omentum.

    abstract::A case of spontaneous internal jugular vein thrombosis associated with distant malignancy is presented. This unusual manifestation of a hypercoagulable state was associated with a low-grade epithelioid leiomyosarcoma of the omentum. It was discovered on a gynecologic oncology service in a patient explored for a pelvic...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章

    doi:10.1016/0090-8258(92)90087-y

    authors: Langlieb AM,Dunton CJ,Carlson JA Jr

    更新日期:1992-10-01 00:00:00

  • First-line treatment regimens and the role of consolidation therapy in advanced ovarian cancer.

    abstract::Current first-line management for advanced ovarian cancer consists of cytoreductive surgery followed by chemotherapy, usually with a platinum/taxane combination. Although this approach has been shown to achieve overall response rates of 70-80% in clinical trials, the majority of patients relapse. A number of different...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章,评审

    doi:10.1016/s0090-8258(03)00472-4

    authors: Stuart GC

    更新日期:2003-09-01 00:00:00

  • Necessity of routine cardiac evaluation in patients receiving pegylated liposomal doxorubicin for gynecologic cancer.

    abstract:OBJECTIVE:Pegylated liposomal doxorubicin (PLD) has similar reported clinical efficacy compared with conventional doxorubicin with less cardiotoxicity. The manufacturer of PLD advises that cardiac function should be evaluated with endomyocardial biopsy, echocardiography or multigated radionucleotide scan (MUGA) pre-tre...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章

    doi:10.1016/j.ygyno.2019.09.009

    authors: Dioun SM,Vilardo N,Goldberg GL,Gressel GM

    更新日期:2019-11-01 00:00:00

  • Minimal change disease in a patient with ovarian papillary serous carcinoma.

    abstract:BACKGROUND:Nephrotic syndrome (NS) is rarely associated with ovarian tumor. Only five cases of NS with pathologic diagnosis have been reported in patients with ovarian tumors. However, the association between minimal change disease (MCD) and ovarian tumor has not been previously reported. CASE:A 55-year-old woman pres...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章

    doi:10.1016/j.ygyno.2004.02.022

    authors: Ryu DR,Yoo TH,Kim YT,Jeong HJ,Cho NH,Kang SW

    更新日期:2004-05-01 00:00:00

  • Resveratrol inhibits ovarian cancer cell adhesion to peritoneal mesothelium in vitro by modulating the production of α5β1 integrins and hyaluronic acid.

    abstract:OBJECTIVE:Resveratrol (Res) is known to inhibit adhesion of numerous malignancies though its effect on an adherence of ovarian cancer cells to peritoneal mesothelium remains undefined. METHODS:To address this issue, ovarian cancer cells (A2780, OVCAR-3, SKOV-3) were subjected to Res (10, 50, 100 μM), and then their ad...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章

    doi:10.1016/j.ygyno.2014.06.022

    authors: Mikuła-Pietrasik J,Sosińska P,Książek K

    更新日期:2014-09-01 00:00:00

  • Cyclin E as a potential therapeutic target in high grade serous ovarian cancer.

    abstract::Cyclin E1 (CCNE1) gene amplification occurs in approximately 20% of ovarian high grade serous carcinoma (HGSC) and is associated with chemotherapy resistance and, in some studies, overall poor prognosis. The role of cyclin E1 in inducing S phase entry relies upon its interactions with cyclin dependent kinases (CDK), s...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章,评审

    doi:10.1016/j.ygyno.2016.07.111

    authors: Kanska J,Zakhour M,Taylor-Harding B,Karlan BY,Wiedemeyer WR

    更新日期:2016-10-01 00:00:00

  • Neuroendocrine (Merkel cell) carcinoma of the vulva: a case report and review of the literature.

    abstract::The clinical and pathologic features of a vulvar neuroendocrine (Merkel cell) neoplasm are presented. Cytologic studies of material obtained from needle aspiration suggested that the tumor was a small cell neoplasm possibly of neuroendocrine derivation. The light-microscopic findings of sheets of small, uniform cells ...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章

    doi:10.1016/0090-8258(85)90053-8

    authors: Copeland LJ,Cleary K,Sneige N,Edwards CL

    更新日期:1985-11-01 00:00:00

  • Platelets promote invasion and induce epithelial to mesenchymal transition in ovarian cancer cells by TGF-β signaling pathway.

    abstract:OBJECTIVE:To test whether platelets could increase invasion potential and initiate EMT in ovarian cancer cells via a TGF-β signaling pathway. METHODS:Blood samples were collected in 69 patients with ovarian cancer, 16 patients with benign ovarian tumor and 64 healthy donors. SK-OV-3 and OVCAR-3 ovarian cancer cells we...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章

    doi:10.1016/j.ygyno.2019.02.026

    authors: Guo Y,Cui W,Pei Y,Xu D

    更新日期:2019-06-01 00:00:00

  • Phase II study of axalimogene filolisbac (ADXS-HPV) for platinum-refractory cervical carcinoma: An NRG oncology/gynecologic oncology group study.

    abstract:OBJECTIVE:Women with persistent, recurrent, and/or metastatic cervical cancer have a poor prognosis. Even with the availability of cisplatin plus paclitaxel and bevacizumab, median overall survival (OS) is only 17.0 months, with median post-progression survival of approximately seven months. We studied the therapeutic ...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章

    doi:10.1016/j.ygyno.2020.06.493

    authors: Huh WK,Brady WE,Fracasso PM,Dizon DS,Powell MA,Monk BJ,Leath CA 3rd,Landrum LM,Tanner EJ,Crane EK,Ueda S,McHale MT,Aghajanian C

    更新日期:2020-09-01 00:00:00

  • MicroRNA signatures of tumor-derived exosomes as diagnostic biomarkers of ovarian cancer.

    abstract:OBJECTIVES:Most ovarian cancer patients are diagnosed at an advanced stage (67%) and prospects for significant improvement in survival reside in early diagnosis. While expression patterns of a recently identified biomarker family, microRNA, appear to be characteristic of tumor type and developmental origin, microRNA pr...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章

    doi:10.1016/j.ygyno.2008.04.033

    authors: Taylor DD,Gercel-Taylor C

    更新日期:2008-07-01 00:00:00

  • Does serum CA-125 level prior to second-look laparotomy for invasive ovarian adenocarcinoma predict size of residual disease?

    abstract::The records of 125 patients with nonmucinous invasive ovarian adenocarcinoma who underwent cytoreductive surgery, cisplatin-based combination chemotherapy, and second-look laparotomy were analyzed to correlate pre-second-look serum CA-125 levels with the size of residual ovarian cancer. The majority of patients with n...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章

    doi:10.1016/0090-8258(90)90359-s

    authors: Patsner B,Orr JW Jr,Mann WJ Jr,Taylor PT,Partridge E,Allmen T

    更新日期:1990-06-01 00:00:00

  • Osseous metaplasia in ovarian papillary serous cystadenocarcinoma.

    abstract::The finding of osseous metaplasia within an ovarian papillary serous cystadenocarcinoma is very rare. A review of the medical literature reveals only two previously published cases where mature bone was found in an ovarian serous cystadenocarcinoma. This report presents an additional case of this unusual phenomenon wh...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章

    doi:10.1016/0090-8258(90)90439-r

    authors: Bosscher J,Barnhill D,O'Connor D,Doering D,Nash J,Park R

    更新日期:1990-11-01 00:00:00

  • Systemic therapy for advanced uterine sarcoma: a systematic review of the literature.

    abstract:OBJECTIVE:To conduct a systematic review of the literature regarding the systemic treatment of advanced uterine sarcoma and provide an evidence-based summary of the available literature. METHODS:MEDLINE, EMBASE, and the Cochrane Library databases were searched. "Uterine sarcoma," "leiomyosarcoma," "mixed mesodermal tu...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章,评审

    doi:10.1016/j.ygyno.2005.01.041

    authors: Kanjeekal S,Chambers A,Fung MF,Verma S

    更新日期:2005-05-01 00:00:00

  • Cadherins, catenins and cell cycle regulators: impact on survival in a Gynecologic Oncology Group phase II endometrial cancer trial.

    abstract:OBJECTIVE:We evaluated the clinical relevance of catenins, cadherins and cell cycle regulators in stage IV or recurrent endometrial carcinoma in a multi-center phase II trial (GOG protocol #119). METHODS:Tissue microarrays of metastatic or recurrent (n=42) tumor were developed and immunohistochemistry was performed. A...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章,多中心研究

    doi:10.1016/j.ygyno.2011.07.005

    authors: Singh M,Darcy KM,Brady WE,Clubwala R,Weber Z,Rittenbach JV,Akalin A,Whitney CW,Zaino R,Ramirez NC,Leslie KK

    更新日期:2011-11-01 00:00:00

  • Serum albumin: its relationship to marrow and renal toxicity from platinum-based combination chemotherapy.

    abstract::One hundred and three patients treated with CAP chemotherapy were evaluated to determine the relationship between low prechemotherapy serum albumin (less than 3 g/dl) and low WBC nadir (less than 2000 cells/mm3). Additionally, the relationship of serum albumin to renal toxicity (delta serum creatinine) was examined. L...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章

    doi:10.1016/0090-8258(90)90315-c

    authors: Belinson JL,Jarrell MA,McClure M,Kulig PM,Badger GJ

    更新日期:1990-04-01 00:00:00

  • Isolated abdominal wound recurrence of an endometrial adenocarcinoma confined to a polyp.

    abstract::An unusual case of Stage IB moderately well-differentiated endometrial adenocarcinoma that was confined to a polyp and recurred only in the abdominal wound is presented. Eighteen months following complete excision, local electron beam therapy, and the institution of hormonal therapy, the patient is alive and without o...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章

    doi:10.1016/0090-8258(86)90089-2

    authors: Barter JF,Hatch KD,Orr JW Jr,Shingleton HM

    更新日期:1986-11-01 00:00:00

  • HLA-DQB1 and cervical cancer in Venezuelan women.

    abstract:BACKGROUND:Cervical cancer represents a major health problem in Venezuela as well as in other Latin American countries. High-risk human papillomavirus (HR-HPV) infection is known as the major risk factor of cervical cancer. However, whether or not a HR-HPV-infected woman progresses to cervical cancer may depend on the ...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章

    doi:10.1016/j.ygyno.2004.10.002

    authors: Dao DD,Sierra-Torres CH,Robazetti SC,de Gomez MN,König R,Lema C,Lester LJ,Au WW,Tyring SK

    更新日期:2005-02-01 00:00:00

  • Quality of life in women treated with neoadjuvant chemotherapy for advanced ovarian cancer: a prospective longitudinal study.

    abstract:OBJECTIVE:The purpose was to examine the outcomes of patients with advanced ovarian cancer treated with neoadjuvant chemotherapy, with a special emphasis on the patients' quality of life (QOL). METHODS:Seventeen patients with advanced ovarian cancer were treated with neoadjuvant chemotherapy based on the extent of dis...

    journal_title:Gynecologic oncology

    pub_type: 临床试验,杂志文章

    doi:10.1006/gyno.2002.6849

    authors: Chan YM,Ng TY,Ngan HY,Wong LC

    更新日期:2003-01-01 00:00:00