The prevalence of HPV16DNA in normal and pathological cervical scrapes using the polymerase chain reaction.


:Human papillomavirus (HPV) has been implicated in the development of cervical cancer. Newly developed molecular techniques have facilitated HPV identification and typing of cervical lesions. Several studies have suggested a strong correlation between HPV16 and intraepithelial cervical neoplasia. However, there is no agreement on this point since HPV16 is detected also in normal cervical tissue. We have amplified the L1 region of HPV16 DNA using the polymerase chain reaction (PCR) technique and analyzed the HPV16 prevalence in cytological scrapes of 201 Italian sexually active patients. One hundred twenty-four of these women had normal cytologic and colposcopic examination, while seventy-seven women had normal or abnormal cytology and a cervical lesion detectable by colposcopy. Within this latter group, 25 of 77 women (32.4%) were positive for HPV16 DNA, compared to 11 of 124 samples (8.87%) from women with normal cytology and colposcopy. The present results confirm the high efficiency of the PCR technique in detecting HPV16 DNA from cervical scrapes and suggest that this analysis should become a method of choice in clinical studies.


Gynecol Oncol


Gynecologic oncology


Pasetto N,Sesti F,De Santis L,Piccione E,Novelli G,Dallapiccola B




Has Abstract


1992-07-01 00:00:00












  • Radiotherapy with concurrent cisplatin-based doublet or weekly cisplatin for cervical cancer: a systematic review and meta-analysis.

    abstract:BACKGROUND:Treatment with weekly cisplatin (CDDP) plus radiotherapy (RT) is the standard regimen for stage IB to stage IVA cervical carcinoma (CC). We performed a systematic review and meta-analysis of published studies to evaluate whether CDDP-based doublet therapy improves survival compared to weekly CDDP plus RT in ...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章,meta分析,评审


    authors: Petrelli F,De Stefani A,Raspagliesi F,Lorusso D,Barni S

    更新日期:2014-07-01 00:00:00

  • Laparoscopic hand-assisted Miami Pouch after pelvic exenteration in 10 steps.

    abstract:BACKGROUND:Improvements in surgical technique of pelvic exenteration have mainly occurred in the reconstructive phase of the procedure. Quality of life seems to be improved when performing continent rather than non-continent urinary diversion [1]. Unfortunately, Miami continent urinary pouch is a surgical technique not...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Angeles MA,Martínez-Gómez C,Martinez A,Ferron G

    更新日期:2018-08-01 00:00:00

  • Absence of PTEN repeat tract mutation in endometrial cancers with microsatellite instability.

    abstract:OBJECTIVE:PTEN, a tumor suppressor gene shown to be frequently mutated in endometrial cancers, has been suggested to be a target of microsatellite instability (MSI)-driven mutagenesis. We set out to investigate the relationship between MSI and PTEN mutation in a large series of primary endometrial carcinomas. METHODS:...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Cohn DE,Basil JB,Venegoni AR,Mutch DG,Rader JS,Herzog TJ,Gersell DJ,Goodfellow PJ

    更新日期:2000-10-01 00:00:00

  • Radiotherapy is not indicated in patients with vulvar squamous cell carcinoma and only one occult intracapsular groin node metastasis.

    abstract:OBJECTIVE:Most guidelines advise no adjuvant radiotherapy in vulvar squamous cell carcinoma and a single occult intracapsular lymph node metastasis. However, several recent studies have questioned the validity of this recommendation. The aim of this study was to analyze the groin recurrence rate in patients with a sing...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: van der Velden J,Pleunis N,Barlow E,Zijlmans H,de Hullu J,Hacker NF,Fons G

    更新日期:2021-01-01 00:00:00

  • A phase II clinical trial of pegylated liposomal doxorubicin and carboplatin plus bevacizumab in patients with platinum-sensitive recurrent ovarian, fallopian tube, or primary peritoneal cancer.

    abstract:OBJECTIVE:To assess the safety and efficacy of pegylated liposomal doxorubicin (PLD), carboplatin, plus bevacizumab in patients with ovarian, fallopian tube, or primary peritoneal cancer. METHODS:Patients with platinum-sensitive, recurrent disease received PLD 30 mg/m(2) and carboplatin area under the curve (AUC) 5 on...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章,多中心研究


    authors: del Carmen MG,Micha J,Small L,Street DG,Londhe A,McGowan T

    更新日期:2012-09-01 00:00:00

  • Correlation of CA125 and CA19-9 serum levels with clinical course and second-look findings in patients with ovarian carcinoma.

    abstract::CA125 and CA19-9 levels were serially evaluated in blood samples from 21 patients during and after integrated surgical and chemotherapeutic treatment for ovarian carcinoma. Serial measurement of CA125 and CA19-9 correlated with clinical course of disease in 89.7 and 72.7% of instances, respectively. The decrease of se...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Fioretti P,Gadducci A,Ferdeghini M,Bartolini T,Bianchi R,Facchini V

    更新日期:1987-11-01 00:00:00

  • Moving forward with actionable therapeutic targets and opportunities in endometrial cancer: A NCI clinical trials planning meeting report.

    abstract::The incidence of endometrial cancer (EC) in the U.S. has been rising, from an estimated annual incidence of 49,560 in 2013 to 61,380 in 2017. Meanwhile, the SEER-based relative survival of women with EC in the U.S. has remained flat [82.3% from 1987 to 1989, 82.8% from 2007 to 2013] and our recent increased understand...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章,评审


    authors: Lheureux S,McCourt C,Rimel BJ,Duska L,Fleming G,Mackay H,Mutch D,Temkin SM,Lynn J,Kohn EC

    更新日期:2018-02-22 00:00:00

  • Classification and regression tree (CART) analysis of endometrial carcinoma: Seeing the forest for the trees.

    abstract:OBJECTIVE:The objectives of the study are to evaluate which clinicopathologic factors influenced overall survival (OS) in endometrial carcinoma and to determine if the surgical effort to assess para-aortic (PA) lymph nodes (LNs) at initial staging surgery impacts OS. METHODS:All patients diagnosed with endometrial can...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Barlin JN,Zhou Q,St Clair CM,Iasonos A,Soslow RA,Alektiar KM,Hensley ML,Leitao MM Jr,Barakat RR,Abu-Rustum NR

    更新日期:2013-09-01 00:00:00

  • Metastasis from a stage IB cervical adenocarcinoma in a transposed ovary: a case report and review of the literature.

    abstract::Adenocarcinoma originating in the endocervix may remain occult for many years. This may result in an extension to the uterine isthmus and corpus, with subsequent dissemination along the pathways of spread of uterine carcinoma, including metastasis to the ovaries. The patient described in this report had a bulky stage ...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章,评审


    authors: Parham G,Heppard MC,DiSaia PJ

    更新日期:1994-12-01 00:00:00

  • Pathways to a cancer-free future: A protocol for modelled evaluations to maximize the future impact of interventions on cervical cancer in Australia.

    abstract:OBJECTIVE:Australia's HPV vaccination and HPV-based cervical screening programs are changing the landscape in cervical cancer prevention. We aim to identify areas which can make the biggest further impact on cervical cancer burden. This protocol describes the first stage of a program of work called Pathways-Cervix that...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Velentzis LS,Smith MA,Simms KT,Lew JB,Hall M,Hughes S,Yuill S,Killen J,Keane A,Butler K,Darlington-Brown J,Hui H,Brotherton JML,Skinner R,Brand A,Roeske L,Heley S,Carter J,Bateson D,Frazer I,Garland SM,Guy R,H

    更新日期:2019-03-01 00:00:00

  • High cyclooxygenase-2 expression in cervical adenocarcinomas.

    abstract:OBJECTIVE:The purpose of this study was to examine the relationships between cyclooxygenase-2 (COX-2) expression and prognostic factors in cervical carcinomas. METHODS:We studied COX-2 expression in 53 women with cervical cancers, including 35 squamous cell carcinomas (SCCs), 1 adenosquamous cell carcinoma (ASCC), and...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Chen YJ,Wang LS,Wang PH,Lai CR,Yen MS,Ng HT,Yuan CC

    更新日期:2003-03-01 00:00:00

  • Phase I study of daily cisplatin and concurrent radiotherapy in patients with cervical carcinoma.

    abstract:OBJECTIVE:Chemoradiation based on cisplatin is the standard treatment for locally advanced cervical carcinoma; however, the optimal scheduling and dosing have still not been established. This study was conducted to determine the maximum-tolerated dose (MTD) of cisplatin for daily administration during pelvic radiothera...

    journal_title:Gynecologic oncology

    pub_type: 临床试验,杂志文章


    authors: Mitsuhashi A,Uno T,Tanaka N,Suzuka K,Tate S,Yamazawa K,Matsui H,Yamamoto S,Ito H,Sekiya S

    更新日期:2005-01-01 00:00:00

  • Endometrial cancer and abdominal wound recurrence.

    abstract::A 65-year-old woman had a total abdominal hysterectomy and bilateral salpingo-oophorectomy through a Pfannenstiel incision for a Stage Ib, Grade 2 endometrial adenocarcinoma with extension to the middle third of the myometrium. No adjuvant treatment was given. She presented 7 years later with a large suprapubic mass a...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Kotwall CA,Kirkbride P,Zerafa AE,Murray D

    更新日期:1994-06-01 00:00:00

  • Prognostic significance of growth characteristics of xenotransplanted ovarian carcinomas into nude mice.

    abstract::Human ovarian carcinomas (n = 215) were xenotransplanted into thymus-aplastic female nu/nu mice (NMRI). Growth of transplants was defined as take-off rate, growth rapidity, and feasibility of passages. In 204 cases these growth characteristics could be correlated with survival time of the individual tumor-donor patien...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Kleine W

    更新日期:1986-09-01 00:00:00

  • Phase II study of concurrent chemoradiotherapy with high-dose-rate intracavitary brachytherapy in patients with locally advanced uterine cervical cancer: efficacy and toxicity of a low cumulative radiation dose schedule.

    abstract:OBJECTIVE:A multicenter phase II trial was conducted to assess the efficacy and toxicity of concurrent chemoradiotherapy (CCRT) with high-dose-rate intracavitary brachytherapy (HDR-ICBT) using a low cumulative prescribed dose schedule in patients with locally advanced uterine cervical cancer. METHODS:The Japanese Gyne...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章,多中心研究


    authors: Toita T,Kitagawa R,Hamano T,Umayahara K,Hirashima Y,Aoki Y,Oguchi M,Mikami M,Takizawa K,Cervical Cancer (Vulva Cancer) Committee of Japanese Gynecologic Oncology Group (JGOG).

    更新日期:2012-08-01 00:00:00

  • Risk of malignancy index in the preoperative evaluation of patients with adnexal masses.

    abstract:OBJECTIVE:The aim of this study was to evaluate the use of a Risk of Malignancy Index (RMI) in primary evaluation of patients with adnexal masses. METHODS:The RMI is based on menopausal status, ultrasonographic findings, and serum CA 125 level. A cutoff level of 200 was chosen as the threshold for referral for central...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Andersen ES,Knudsen A,Rix P,Johansen B

    更新日期:2003-07-01 00:00:00

  • A prospective study evaluating the clinical relevance of a chemoresponse assay for treatment of patients with persistent or recurrent ovarian cancer.

    abstract:OBJECTIVE:Use of in vitro chemoresponse assays for informing effective treatment selection is a compelling clinical question and a topic of debate among oncologists. A prospective study was conducted evaluating the use of a chemoresponse assay in recurrent ovarian cancer patients. METHODS:Women with persistent or recu...

    journal_title:Gynecologic oncology

    pub_type: 临床试验,杂志文章


    authors: Rutherford T,Orr J Jr,Grendys E Jr,Edwards R,Krivak TC,Holloway R,Moore RG,Puls L,Tillmanns T,Schink JC,Brower SL,Tian C,Herzog TJ

    更新日期:2013-11-01 00:00:00

  • Histopathologic behavior of endometrial hyperplasia during tamoxifen therapy for breast cancer.

    abstract:OBJECTIVE:The purpose of the present study is to prospectively evaluate the effects of tamoxifen on the pathological behavior of endometrial hyperplasias without atypia, diagnosed before the start of adjuvant endocrine therapy, in menopausal patients suffering from breast cancer. METHODS:Twenty-six patients suffering ...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Garuti G,Cellani F,Centinaio G,Sita G,Nalli G,Luerti M

    更新日期:2006-05-01 00:00:00

  • Experience with the P.A.S.-PORT venous access device in patients with gynecologic malignancies.

    abstract::Experience with the P.A.S.-PORT, a peripherally implanted central venous access device, is evaluated in a retrospective review of 154 patients from July 1991 to June 1994. Blood could not be aspirated from six patients. Complications included temporary minor thrombophlebitis in seven patients (4.5%), symptomatic axill...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Deppe G,Kahn ML,Malviya VK,Malone JM Jr,Christensen CW

    更新日期:1996-09-01 00:00:00

  • Long-term survival in an ovarian cancer patient with brain metastases.

    abstract:BACKGROUND:Central nervous system (CNS) metastases from ovarian adenocarcinoma are uncommon. The long-term prognosis for these patients is poor, with studies reporting a mean survival of less than 12 months. CASE:We present a case involving a 57-year-old woman diagnosed and treated for primary ovarian cancer in 1994. ...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Micha JP,Goldstein BH,Hunter JV,Rettenmaier MA,Brown JV

    更新日期:2004-03-01 00:00:00

  • Combined laparoscopic and vaginal surgery for the management of two cases of stage I endometrial cancer.

    abstract::Two postmenopausal patients with stage I adenocarcinoma of the endometrium who were managed with a combined laparoscopic and vaginal approach are presented. Surgical-pathologic staging was performed laparoscopically, with exploration of the abdomen and procurement of peritoneal cytology and pelvic and para-aortic lymp...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Childers JM,Surwit EA

    更新日期:1992-04-01 00:00:00

  • Detection of chromogranin in argyrophil cells of endometrial carcinoma.

    abstract::Normal and neoplastic endometrial tissues were examined immunohistochemically for chromogranin which was shown to be a specific marker for neuroendocrine cells. All 20 normal endometriums and 20 endometrial carcinomas without argyrophilia were chromogranin negative. Endometrial carcinomas with argyrophilia tested were...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Ueda G,Nishino T,Saito J,Abe Y,Shimizu H,Tanizawa O

    更新日期:1987-06-01 00:00:00

  • C-reactive protein and 6-keto prostaglandin F 1-alpha in patients with gynecologic cancer.

    abstract::C-reactive protein (CRP) is an acute phase reactant that appears to have a variety of biologic effects, including stimulation of prostaglandin production by peripheral blood monocytes. Both CRP and 6-keto prostaglandin F 1-alpha (6-keto PGF1-alpha) have been noted to be elevated in the sera of patients with malignant ...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Terada K,Hayashi G,Hokama Y

    更新日期:1990-02-01 00:00:00

  • p16INK4A in routine practice as a marker of cervical epithelial neoplasia.

    abstract:OBJECTIVE:The aim of this study was to validate the role of p16(INK4a) immunocytochemistry as a marker of cervical epithelial neoplasia in cytology and biopsy samples. METHODS:During the period of 4 years a total of 501 immunocytochemistry stains in cytology and biopsy samples were performed. The cytology smears were ...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Kurshumliu F,Thorns C,Gashi-Luci L

    更新日期:2009-10-01 00:00:00

  • Matrix metalloproteinase 2 immunoreactive protein appears early in cervical epithelial dedifferentiation.

    abstract:OBJECTIVE:Expression of the immunoreactive protein of matrix metalloproteinase 2 (MMP-2) was studied in cervical tumors representing various stages of cell atypia and differentiation. In this study, we evaluated whether the expression of MMP-2 is an early or late event in the process of dedifferentiation and cancer pro...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Talvensaari A,Apaja-Sarkkinen M,Höyhtyä M,Westerlund A,Puistola U,Turpeenniemi T

    更新日期:1999-03-01 00:00:00

  • Primary biliary carcinoma with metastasis to the ovary.

    abstract::The ovary is a relatively frequent site of metastasis from malignant neoplasia arising elsewhere in the body, the majority of these originating from the GI tract. The best known tumor of this type is signet-ring cell adenocarcinoma (Krukenberg tumor) of gastric origin. The gall bladder and bile ducts are rare sources ...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章,评审


    authors: Lashgari M,Behmaram B,Hoffman JS,Garcia J

    更新日期:1992-11-01 00:00:00

  • Experience with pelvic washings in stage I and II endometrial carcinoma.

    abstract::Pelvic washings for cytologic analysis have become an accepted diagnostic procedure in the management of endometrial carcinoma. A retrospective study was performed in 163 consecutive patients with FIGO Stage I and II endometrial carcinoma to assess the significance and value of the washings. Abnormal results were obta...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Kennedy AW,Peterson GL,Becker SN,Nunez C,Webster KD

    更新日期:1987-09-01 00:00:00

  • Clinical characteristics and outcomes in elderly women with BRCA1 and BRCA2 mutations.

    abstract:OBJECTIVE:Describe clinical characteristics and risk reducing strategies utilized among women with a BRCA mutation who lived to age 75 and above. METHODS:A retrospective study of women with BRCA mutations identified from 1995 to 2015 in a California health care system. From a database of 1189 women, 69 participants we...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Salyer C,Kobelka C,Barrie A,Weintraub MR,Powell CB

    更新日期:2019-08-01 00:00:00

  • Loop electrocautery excisional procedure: therapeutic effectiveness as an ablation and a conization equivalent.

    abstract::This study was designed to compare the effectiveness of loop excision procedures as ablation and conization equivalents, and their use in recurrent cervical high-grade dysplasia patients. We retrospectively reviewed patients undergoing loop procedures between January 1992 and April 1994. Conization equivalents were de...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Gold M,Dunton CJ,Murray J,Macones G,Hanau C,Carlson JA Jr

    更新日期:1996-05-01 00:00:00

  • Immunohistochemical detection of somatostatin receptors in human ovarian tumors.

    abstract:OBJECTIVE:It is well established that many human tumors can express somatostatin receptors. This is the molecular basis for the application of long-acting somatostatin analogues for therapeutic and diagnostic purposes. However, there are discrepancies in earlier studies regarding the prevalence of somatostatin receptor...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Schulz S,Schmitt J,Quednow C,Roessner A,Weise W

    更新日期:2002-02-01 00:00:00