Analysis and succession of nitrifying bacteria community structure in sequencing biofilm batch reactor.

Abstract:

:To reveal the succession procedure of ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB) community structure in sequencing biofilm batch reactor (SBBR), the molecular biological techniques of denaturing gradient gel electrophoresis (DGGE), cloning, and real-time PCR were applied. DGGE showed that the structural diversity of the bacterial community increased during the biofilm formation period, and some kinds of populations had been highly preponderant consistently. The results of cloning and sequencing revealed that Nitrosomonas was the dominant species. The real-time PCR analysis indicated that the amount of the AOB increased significantly after the cultivation period, and the NOB gradually decreased. The AOB content on the 25th day was 17 times that of the 6th day. It also showed the biofilm formed successfully with accumulating nitrite and prepared to achieve the achievement of simultaneous nitrification and denitrification in SBBR. Furthermore, the ammonia-oxidizing rate was in correspondence with the NH4 (+)-N removal efficiency.

authors

Wang J,Zhang C,Rong H

doi

10.1007/s00253-014-5537-6

subject

Has Abstract

pub_date

2014-05-01 00:00:00

pages

4581-7

issue

10

eissn

0175-7598

issn

1432-0614

journal_volume

98

pub_type

杂志文章
  • Gongronella sp. w5 elevates Coprinopsis cinerea laccase production by carbon source syntrophism and secondary metabolite induction.

    abstract::When sucrose was used as the carbon source, the Basidiomycete Coprinopsis cinerea showed poor growth and low laccase activity in pure culture, but greatly enhanced the level of laccase activity (>1800 U/L) during coculture with the Mucoromycete Gongronella sp. w5. As a result, the mechanism of laccase overproduction i...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-018-9469-4

    authors: Hu J,Zhang Y,Xu Y,Sun Q,Liu J,Fang W,Xiao Y,Kües U,Fang Z

    更新日期:2019-01-01 00:00:00

  • Medicinal mushroom modulators of molecular targets as cancer therapeutics.

    abstract::Empirical approaches to discover anticancer drugs and cancer treatments have made limited progress in the past several decades in finding a cure for cancer. The expanded knowledge of the molecular basis of tumorigenesis and metastasis, together with the inherently vast structural diversity of natural compounds found i...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-004-1787-z

    authors: Zaidman BZ,Yassin M,Mahajna J,Wasser SP

    更新日期:2005-06-01 00:00:00

  • Comparative studies on similarities and differences of cyclodipeptide oxidases for installation of C-C double bonds at the diketopiperazine ring.

    abstract::Cyclodipeptide oxidases (CDOs) perform dehydrogenations on diketopiperazines and play an important role in the cyclodipeptide diversification. In this study, we investigated the two known CDOs AlbA/B and Ndas_1146/7 and one new member, CDO-Np. LC-MS monitoring of 32 cyclodipeptide biotransformations in E. coli reveale...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-020-10392-7

    authors: Mikulski L,Schäfer J,Brockmeyer K,Kraut R,Li SM

    更新日期:2020-03-01 00:00:00

  • Induction of a photomixotrophic plant cell culture of Helianthus annuus and optimization of culture conditions for improved α-tocopherol production.

    abstract::Tocopherols, collectively known as vitamin E, are lipophilic antioxidants, which are synthesized only by photosynthetic organisms. Due to their enormous potential to protect cells from oxidative damage, tocopherols are used, e.g., as nutraceuticals and additives in pharmaceuticals. The most biologically active form of...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-013-5431-7

    authors: Geipel K,Song X,Socher ML,Kümmritz S,Püschel J,Bley T,Ludwig-Müller J,Steingroewer J

    更新日期:2014-03-01 00:00:00

  • Novel osmotic stress control strategy for improved pneumocandin B0 production in Glarea lozoyensis combined with a mechanistic analysis at the transcriptome level.

    abstract::Pneumocandin B0, the precursor of the antifungal drug caspofungin, is a secondary metabolite of the fungus Glarea lozoyensis. In this study, we investigated the effects of mannitol as the sole carbon source on pneumocandin B0 production by G. lozoyensis. The osmotic pressure is more important in enhancing pneumocandin...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-018-9440-4

    authors: Song P,Huang B,Zhang S,Zhang K,Yuan K,Ji X,Ren L,Wen J,Huang H

    更新日期:2018-12-01 00:00:00

  • Intracellular azo decolorization is coupled with aerobic respiration by a Klebsiella oxytoca strain.

    abstract::Reduction of azo dye methyl red coupled with aerobic respiration by growing cultures of Klebsiella oxytoca GS-4-08 was investigated. In liquid media containing dye and 0.6 % glucose in a mineral salts base, 100 mg l(-1) of the dye are completely removed in 3 h under shaking conditions. The dye cannot be aerobically de...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-014-6161-1

    authors: Yu L,Zhang XY,Xie T,Hu JM,Wang S,Li WW

    更新日期:2015-03-01 00:00:00

  • Population dynamics of free-floating and attached bacteria in a styrene-degrading biotrickling filter analyzed by denaturing gradient gel electrophoresis.

    abstract::Population dynamics was studied in a 52-l biotrickling filter (BTF) operated for 182 days and used to clean air contaminated with styrene vapors. In the BTF, biomass grew either as free-floating (planktonic) or attached (sessile) microorganisms. PCR-amplified 16S rDNA fragments from planktonic and sessile cells within...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-002-1039-z

    authors: Tresse O,Lorrain MJ,Rho D

    更新日期:2002-08-01 00:00:00

  • Production of Lactobacillus kefir cells for asymmetric synthesis of a 3,5-dihydroxycarboxylate.

    abstract::An efficient fedbatch process for the production of Lactobacillus kefir DSM 20587 cells was developed. An improvement in space time yield of 270% (3.7 g(DCW) l(-1)-day(-1)) and in final enzyme activity of 440% (9.1 U/ml) was achieved on a 150 l scale by controlling the oxygen transfer rate to 7--9 mmol l(-1) h(-1). Th...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-004-1837-6

    authors: Pfruender H,Amidjojo M,Hang F,Weuster-Botz D

    更新日期:2005-06-01 00:00:00

  • Microbiota composition of captive bluestreak cleaner wrasse Labroides dimidiatus (Valenciennes, 1839).

    abstract::The Labroides dimidiatus is one of the most traded marine ornamental fishes worldwide, yet not much is known about the microflora associated with this fish. This study is designed to investigate the bacteria composition associated with captive L. dimidiatus and its surrounding aquarium water. The fish and carriage wat...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-020-10781-y

    authors: Nurul AAN,Danish-Daniel AM,Okomoda VT,Asma NA

    更新日期:2020-09-01 00:00:00

  • Double Candida antarctica lipase B co-display on Pichia pastoris cell surface based on a self-processing foot-and-mouth disease virus 2A peptide.

    abstract::To develop a high efficiency Candida antarctica lipase B (CALB) yeast display system, we linked two CALB genes fused with Sacchromyces cerevisiae cell wall protein genes, the Sed1 and the 3'-terminal half of Sag1, separately by a 2A peptide of foot-and-mouth disease virus (FMDV) in a single open reading frame. The CAL...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-012-4264-0

    authors: Sun YF,Lin Y,Zhang JH,Zheng SP,Ye YR,Liang XX,Han SY

    更新日期:2012-12-01 00:00:00

  • Metal resistance in acidophilic microorganisms and its significance for biotechnologies.

    abstract::Extremely acidophilic microorganisms have an optimal pH of <3 and are found in all three domains of life. As metals are more soluble at acid pH, acidophiles are often challenged by very high metal concentrations. Acidophiles are metal-tolerant by both intrinsic, passive mechanisms as well as active systems. Passive me...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-014-5982-2

    authors: Dopson M,Holmes DS

    更新日期:2014-10-01 00:00:00

  • A simple method for isolation and construction of markerless cyanobacterial mutants defective in acyl-acyl carrier protein synthetase.

    abstract::Cyanobacterial mutants defective in acyl-acyl carrier protein synthetase (Aas) secrete free fatty acids (FFAs) into the external medium and hence have been used for the studies aimed at photosynthetic production of biofuels. While the wild-type strain of Synechocystis sp. PCC 6803 is highly sensitive to exogenously ad...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-016-7850-8

    authors: Kojima K,Keta S,Uesaka K,Kato A,Takatani N,Ihara K,Omata T,Aichi M

    更新日期:2016-12-01 00:00:00

  • Comparative proteomic analysis: SclR is importantly involved in carbohydrate metabolism in Aspergillus oryzae.

    abstract::The helix-loop-helix (HLH) family of transcriptional factors is a key player in a wide range of developmental processes in organisms from mammals to microbes. We previously identified the bHLH transcription factor SclR in Aspergillus oryzae and found that the loss of SclR function led to significant phenotypic changes...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-017-8588-7

    authors: Jin FJ,Han P,Zhuang M,Zhang ZM,Jin L,Koyama Y

    更新日期:2018-01-01 00:00:00

  • Isolation of a novel high erythritol-producing Pseudozyma tsukubaensis and scale-up of erythritol fermentation to industrial level.

    abstract::This study isolated a novel erythritol-producing yeast strain, which is capable of growth at high osmolarity. Characteristics of the strain include asexual reproduction by multilateral budding, absence of extracellular starch-like compounds, and a negative Diazonium blue B color reaction. Phylogenetic analysis based o...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-009-1871-5

    authors: Jeya M,Lee KM,Tiwari MK,Kim JS,Gunasekaran P,Kim SY,Kim IW,Lee JK

    更新日期:2009-05-01 00:00:00

  • Biocatalytic production of Ɛ-caprolactone using Geotrichum candidum cells immobilized on functionalized silica.

    abstract::Immobilization of the Geotrichum candidum (CCT 1205) cell with functionalized silica creates promising biocatalysts for production of ɛ-caprolactone. The results obtained by immobilization of the whole cell on SiO2-NH2 and SiO2-SH supports indicate that the presence of reactive functional groups on the support may pro...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-020-10875-7

    authors: Silva ALP,da Silva Caridade TN,Magalhães RR,de Sousa KT,de Sousa CC,Vale JA

    更新日期:2020-10-01 00:00:00

  • Biodegradation and kinetic analysis of phthalates by an Arthrobacter strain isolated from constructed wetland soil.

    abstract::A bacterial strain C21 isolated from constructed wetland soil was identified as Arthrobacter sp. based on 16S rRNA gene sequence analysis and physio-biochemical characteristics and was capable of utilizing di-n-butyl phthalate (DBP) as a carbon and energy source for growth. Strain C21 can also utilize other phthalates...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-014-5568-z

    authors: Wen ZD,Gao DW,Wu WM

    更新日期:2014-05-01 00:00:00

  • Improving extracellular protein production in Escherichia coli by overexpressing D,D-carboxypeptidase to perturb peptidoglycan network synthesis and structure.

    abstract::Most recombinant proteins in Escherichia coli are not efficiently secreted to the extracellular space. Structural stabilisation of the cell wall is essential for extracellular protein production in E. coli, for which D,D-carboxypeptidases are essential. Herein, we perturbed the peptidoglycan structure of the E. coli c...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-018-9510-7

    authors: Yang H,Hu J,Lu X,Wang F,Shen W,Hu W,Wang L,Chen X,Liu L

    更新日期:2019-01-01 00:00:00

  • Statistical optimization of medium components for the production of Antrodia cinnamomea AC0623 in submerged cultures.

    abstract::The nutritional medium requirement for biomass and triterpenoid production by Antrodia cinnamomea AC0623 strain was optimized. Box-Behnken was applied to optimize biomass and triterpenoid production. According to response surface methodology (RSM), the optimum concentrations of N-source were determined. The results in...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-006-0325-6

    authors: Chang CY,Lee CL,Pan TM

    更新日期:2006-10-01 00:00:00

  • Involvement of the NADH oxidase-encoding noxA gene in oxidative stress responses in Corynebacterium glutamicum.

    abstract::Corynebacterium glutamicum ORF NCgl0328, designated noxA, encodes an NADH oxidase enzyme. The noxA gene, which was preferentially expressed in the log growth phase, was found to be under the control of the whcA, whcB, and whcE genes, which play regulatory roles in cells under oxidative stress. While noxA transcription...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-014-6327-x

    authors: Park JC,Kim Y,Lee HS

    更新日期:2015-02-01 00:00:00

  • Molecular cloning, characterization, and heterologous expression of a new κ-carrageenase gene from marine bacterium Zobellia sp. ZM-2.

    abstract::κ-Carrageenases exhibit apparent distinctions in gene sequence, molecular weight, enzyme properties, and posttranslational processes. In this study, a new κ-carrageenase gene named cgkZ was cloned from the marine bacterium Zobellia sp. ZM-2. The gene comprised an open reading frame of 1,638 bp and encoded 545 amino ac...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-013-5215-0

    authors: Liu Z,Li G,Mo Z,Mou H

    更新日期:2013-12-01 00:00:00

  • Opportunities for broadening the application of cell wall lytic enzymes.

    abstract::In light of emerging antibiotic resistance, bacterial cell wall lytic enzymes are promising antimicrobial agents that degrade bacterial peptidoglycan while specifically recognizing the target bacterium. The efficacy of lytic enzymes against several multi-drug-resistant pathogens infecting humans has led to many effort...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-020-10862-y

    authors: Bhagwat A,Mixon M,Collins CH,Dordick JS

    更新日期:2020-11-01 00:00:00

  • Efficient transient expression of human GM-CSF protein in Nicotiana benthamiana using potato virus X vector.

    abstract::The human granulocyte macrophage colony-stimulating factor (GM-CSF) is a glycoprotein with important clinical applications for the treatment of neutropenia and aplastic anemia and reducing infections associated with bone marrow transplants. We evaluated the potential for using a potato virus X (PVX) viral vector syste...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-005-0305-2

    authors: Zhou F,Wang ML,Albert HH,Moore PH,Zhu YJ

    更新日期:2006-10-01 00:00:00

  • Mutational analyses of Cry protein block7 polypeptides that facilitate the formation of protein inclusion in Escherichia coli.

    abstract::4AaCter is the polypeptide from the C-terminal extension of mosquitocidal Cry4Aa toxin, and facilitates formation of protein inclusion in Escherichia coli. It has been demonstrated that the use of 4AaCter as a peptide tag results in the efficient production of heterologous protein in E. coli. It has also been demonstr...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-011-3216-4

    authors: Hayakawa T,Shimizu Y,Ishida T,Sakai H

    更新日期:2011-06-01 00:00:00

  • High-efficiency acetone-butanol-ethanol production and recovery in non-strict anaerobic gas-stripping fed-batch fermentation.

    abstract::Conventional acetone-butanol-ethanol (ABE) fermentation coupled with gas stripping is conducted under strict anaerobic conditions. In this work, a fed-batch ABE fermentation integrated with gas stripping (FAFIGS) system using a non-strict anaerobic butanol-producing symbiotic system, TSH06, was investigated for the ef...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-017-8520-1

    authors: Lin Z,Liu H,Yan X,Zhou Y,Cheng K,Zhang J

    更新日期:2017-11-01 00:00:00

  • Production of L-phenylacetylcarbinol by microbial transformation in polyethylene glycol-induced cloud point system.

    abstract::Microbial transformation of benzaldehyde into L: -phenylacetylcarbinol by whole cell Saccharomyces cerevisiae has been carried out in a novel polyethylene glycol (PEG)-induced cloud point system. The system is composed of 80 g PEG 20,000, 75 ml Triton X-100, 20 g peptone, 10 g yeast extract, 25 g glucose, 1 g MgSO(4)....

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-007-1304-2

    authors: Zhang W,Wang Z,Li W,Zhuang B,Qi H

    更新日期:2008-02-01 00:00:00

  • Aerobic dechlorination of low-chlorinated biphenyls by bacterial biofilms in packed-bed batch bioreactors.

    abstract::Cells of an aerobic three-membered bacterial co-culture, designated as ECO3, capable of cometabolizing and aerobically dechlorinating low-chlorinated biphenyls in the presence of biphenyl, were immobilized on Manville silica beads, on frosted-glass beads and on polyurethane foam cubes in packed-bed bioreactors continu...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF00578472

    authors: Fava F,Di Gioia D,Marchetti L,Quattroni G

    更新日期:1996-05-01 00:00:00

  • Current state and perspectives of truffle genetics and sustainable biotechnology.

    abstract::Mycorrhizal fungi belonging to the genus Tuber produce, after the establishment of a productive interaction with a plant host, hypogeous fruitbodies of great economic value known as ''truffles''. This review summarizes the state of art on life cycle, genetic, and biotechnological investigations of Tuber spp. The ascoc...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-006-0519-y

    authors: Poma A,Limongi T,Pacioni G

    更新日期:2006-09-01 00:00:00

  • Shifts of microbial communities of wheat (Triticum aestivum L.) cultivation in a closed artificial ecosystem.

    abstract::The microbial communities of plant ecosystems are in relation to plant growing environment, but the alteration in biodiversity of rhizosphere and phyllosphere microbial communities in closed and controlled environments is unknown. The purpose of this study is to analyze the change regularity of microbial communities w...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-016-7317-y

    authors: Qin Y,Fu Y,Dong C,Jia N,Liu H

    更新日期:2016-05-01 00:00:00

  • Process characterization and influence of alternative carbon sources and carbon-to-nitrogen ratio on organic acid production by Aspergillus oryzae DSM1863.

    abstract::L-Malic acid and fumaric acid are C4 dicarboxylic organic acids and considered as promising chemical building blocks. They can be applied as food preservatives and acidulants in rust removal and as polymerization starter units. Molds of the genus Aspergillus are able to produce malic acid in large quantities from gluc...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-014-5614-x

    authors: Ochsenreither K,Fischer C,Neumann A,Syldatk C

    更新日期:2014-06-01 00:00:00

  • Novel targets and strategies to combat borreliosis.

    abstract::Lyme borreliosis is a bacterial infection that can be spread to humans by infected ticks and may severely affect many organs and tissues. Nearly four decades have elapsed since the discovery of the disease agent called Borrelia burgdorferi. Although there is a plethora of knowledge on the infectious agent and thousand...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-020-10375-8

    authors: Strnad M,Grubhoffer L,Rego ROM

    更新日期:2020-03-01 00:00:00