Statistical optimization of medium components for the production of Antrodia cinnamomea AC0623 in submerged cultures.

abstract：:In order to produce sophorolipids from whey, thereby lowering the lactose content and biological oxygen demand, a two-step batch cultivation process was developed including medium sterilization by filtration. In the first step, whey was sterilized by a combination of crossflow and sterile filtration. Because the sopho...

journal_title：Applied microbiology and biotechnology

authors： Daniel HJ,Otto RT,Binder M,Reuss M,Syldatk C

更新日期：1999-01-01 00:00:00

abstract：:Elimination of acetate overflow in aerobic cultivation of Escherichia coli would improve many bioprocesses as acetate accumulation in the growth environment leads to numerous negative effects, e.g. loss of carbon, inhibition of growth, target product synthesis, etc. Despite many years of studies, the mechanism and reg...

journal_title：Applied microbiology and biotechnology

authors： Peebo K,Valgepea K,Nahku R,Riis G,Oun M,Adamberg K,Vilu R

更新日期：2014-06-01 00:00:00

abstract：:A cDNA of a structural gene encoding pyranose 2-oxidase (P2O) from Trametes ochracea strain MB49 was cloned into Escherichia coli strain BL21(DE3) on a multicopy plasmid under the control of the trc promoter. Synthesis of P2O was studied in batch cultures in LB or M9-based mineral medium at 28 degrees C. While there w...

journal_title：Applied microbiology and biotechnology

authors： Vecerek B,Maresová H,Kocanová M,Kyslík P

更新日期：2004-05-01 00:00:00

abstract：:Several studies support the use of probiotics for the treatment of minor gastrointestinal problems in infants. Positive effects on newborn colics have been evidenced after administration of Lactobacillus strains, whereas no studies have been reported regarding the use of bifidobacteria for this purpose. This work was ...

journal_title：Applied microbiology and biotechnology

authors： Aloisio I,Santini C,Biavati B,Dinelli G,Cencič A,Chingwaru W,Mogna L,Di Gioia D

更新日期：2012-12-01 00:00:00

abstract：:Staphylococcus aureus-induced bovine mastitis causes significant losses to the dairy industry and available vaccines do not confer adequate protection. As a more attractive alternative, we propose the use of antibody (Ab) therapy. In our previous study, we constructed a bovine single-chain variable fragment (scFv) Ab ...

journal_title：Applied microbiology and biotechnology

authors： Wang M,Wang T,Guan Y,Wang F,Zhu J

更新日期：2019-02-01 00:00:00

abstract：:The human cell line rF2N78 produces an antibody with a high galactosylation ratio which resembles human IgG. However, it has been observed that the aglycosylated antibody starts to appear when glucose is depleted. To determine whether glucose depletion is a main cause for aglycosylation of the antibody, fed-batch cult...

journal_title：Applied microbiology and biotechnology

authors： Seo JS,Min BS,Kim YJ,Cho JM,Baek E,Cho MS,Lee GM

更新日期：2014-04-01 00:00:00

abstract：:Conductive materials can facilitate syntrophic methane (CH4) production by improving direct interspecies electron transfer. The effect of a conductive material, ferroferric oxide (Fe3O4), on the anaerobic treatment of tryptone-based high-strength wastewater was investigated in two anaerobic sequencing batch reactors (...

journal_title：Applied microbiology and biotechnology

authors： Yin Q,He K,Liu A,Wu G

更新日期：2017-05-01 00:00:00

abstract：:We report the study of the dynamics of substrate utilization by the genetic modified strain Yarrowia lipolytica H222-S4(p67ICL1) T5. In contrast to its wild-type equivalent, this recombinant strain is able to excrete the sucrose cleaving enzyme invertase. Both the sucrose degradation rate and the glucose and fructose ...

journal_title：Applied microbiology and biotechnology

authors： Moeller L,Zehnsdorf A,Aurich A,Bley T,Strehlitz B

更新日期：2012-02-01 00:00:00

abstract：:Inonotus obliquus (Fr.) Pilat is a white rot fungus belonging to the family Hymenochaetaceae in the Basidiomycota. In nature, this fungus rarely forms a fruiting body but usually an irregular shape of sclerotial conk called 'Chaga'. Characteristically, I. obliquus produces massive melanins released to the surface of C...

journal_title：Applied microbiology and biotechnology

authors： Zheng W,Miao K,Liu Y,Zhao Y,Zhang M,Pan S,Dai Y

更新日期：2010-07-01 00:00:00

abstract：:This paper reports a novel integrated metabolic pathway of amino acid production through the utilization of phenol in Corynebacterium glutamicum. In the presence of 8.5 mM phenol, the level of glutamate and proline production increased up to 1.2- and 14.7-fold, respectively, compared to the control condition. In addit...

journal_title：Applied microbiology and biotechnology

authors： Lee SY,Kim YH,Min J

更新日期：2010-01-01 00:00:00

abstract：:The utilization of monochlorobenzoate isomers (2-, 3- and 4-chlorobenzoate) by anaerobic microbial consortia in River Nile sediments was systematically evaluated under denitrifying, Fe-reducing, sulfidogenic and methanogenic conditions. Loss of all three chlorobenzoates was noted in denitrifying cultures; furthermore,...

journal_title：Applied microbiology and biotechnology

authors： Kazumi J,Häggblom MM,Young LY

更新日期：1995-10-01 00:00:00

abstract：:There is emerging interest in the anaerobic degradation of crude oil. However, there is limited knowledge about the geochemical effects and microbiological activities for it. A mixture of anaerobic sludge and the production water from an oil well was used as an inoculum to construct four consortia, which were incubate...

journal_title：Applied microbiology and biotechnology

authors： Xiong S,Li X,Chen J,Zhao L,Zhang H,Zhang X

更新日期：2015-02-01 00:00:00

abstract：:A gene encoding beta-glucanase activity from Bacillus amyloliquefaciens was subcloned in both orientations into plasmid shuttle vector pSA3. In only one orientation could a co-integrate be generated with the conjugative plasmid pVA797. The plasmid co-integrate was conjugated into Lactobacillus helveticus strain CNRZ45...

journal_title：Applied microbiology and biotechnology

authors： Thompson K,Collins M

更新日期：1991-06-01 00:00:00

abstract：:The glycopeptide teicoplanin isolated from the fermentation broth of Actinoplanes teichomyceticus is used to treat serious Gram-positive bacterial infections that are resistant to other antibiotics, e.g. beta-lactams. The long time frame and progressively broader clinical use of teicoplanin has eventually led to the e...

journal_title：Applied microbiology and biotechnology

authors： Jung HM,Jeya M,Kim SY,Moon HJ,Kumar Singh R,Zhang YW,Lee JK

更新日期：2009-09-01 00:00:00

abstract：:Myo-inositol is important for Streptomyces growth and morphological differentiation. Genomic sequence analysis revealed a myo-inositol catabolic gene cluster in Streptomyces coelicolor. Disruption of the corresponding genes in this cluster abolished the bacterial growth on myo-inositol as a single carbon source. The t...

journal_title：Applied microbiology and biotechnology

authors： Yu L,Li S,Gao W,Pan Y,Tan H,Liu G

更新日期：2015-04-01 00:00:00

abstract：:Xanthomonas campestris pv. campestris, the causal agent of black-rot disease of cruciferous plants, and an important industrial microbe, was able to express the Escherichia coli beta-glucuronidase reporter gene (uidA) when fused to the E. coli lactose operon promoter on a wide-host-range plasmid vector. The gene fusio...

journal_title：Applied microbiology and biotechnology

authors： Soby SD,Daniels MJ

更新日期：1996-12-01 00:00:00

abstract：:Poly(lactate-co-3-hydroxybutyrate) (P(LA-co-3HB)) was previously produced from xylose in engineered Escherichia coli. The aim of this study was to increase the polymer productivity and LA fraction in P(LA-co-3HB) using two metabolic engineering approaches: (1) deletions of competing pathways to lactate production and ...

journal_title：Applied microbiology and biotechnology

authors： Nduko JM,Matsumoto K,Ooi T,Taguchi S

更新日期：2014-03-01 00:00:00

abstract：:Due to the emerging environmental issues related to heavy metals, concern about the soil quality of farming lands near manufacturing district is increasing. Investigating the function of soil microorganisms exposed to long-term heavy metal contamination is meaningful and important for agricultural soil utilization. Th...

journal_title：Applied microbiology and biotechnology

authors： Deng L,Zeng G,Fan C,Lu L,Chen X,Chen M,Wu H,He X,He Y

更新日期：2015-10-01 00:00:00

abstract：:The rational modification of the actinomycetes genomes has a variety of applications in research, medicine, and biotechnology. The use of site-specific recombinases allows generation of multiple mutations, large DNA deletions, integrations, and inversions and may lead to significant progress in all of these fields. De...

journal_title：Applied microbiology and biotechnology

authors： Myronovskyi M,Luzhetskyy A

更新日期：2013-06-01 00:00:00

abstract：:The genes encoding two conjugated polyketone reductases (CPR-C1, CPR-C2) of Candida parapsilosis IFO 0708 were cloned and sequenced. The genes encoded a total of 304 and 307 amino acid residues for CPR-C1 and CPR-C2, respectively. The deduced amino acid sequences of the two enzymes showed high similarity to each other...

journal_title：Applied microbiology and biotechnology

authors： Kataoka M,Delacruz-Hidalgo AR,Akond MA,Sakuradani E,Kita K,Shimizu S

更新日期：2004-04-01 00:00:00

abstract：:On a near future, bio-based economy will assume a key role in our lives. Lignocellulosic materials (e.g., agroforestry residues, industrial/solid wastes) represent a cheaper and environmentally friendly option to fossil fuels. Indeed, following suitable processing, they can be metabolized by different microorganisms t...

journal_title：Applied microbiology and biotechnology

authors： Gomes D,Rodrigues AC,Domingues L,Gama M

更新日期：2015-05-01 00:00:00

abstract：:Chemical mutagenesis of the phototrophic green microalga Parietochloris incisa, producing high amounts of arachidonic acid (ARA), resulted in selection of a mutant deficient in ARA and rich in dihomo-γ-linolenic acid (DGLA) and thus ∆5 desaturase defective. The mutagenesis produced a nonsense mutation in the ∆5 desatu...

journal_title：Applied microbiology and biotechnology

authors： Iskandarov U,Khozin-Goldberg I,Cohen Z

更新日期：2011-04-01 00:00:00

abstract：:Two enzymatic systems were developed for the efficient synthesis of glycoside products of α-mangostin, a natural xanthonoid exhibiting anti-oxidant, antibacterial, anti-inflammatory, and anticancer activities. In these systems, one-pot reactions for the synthesis of UDP-α-D-glucose and UDP-α-D-2-deoxyglucose were modi...

journal_title：Applied microbiology and biotechnology

authors： Le TT,Pandey RP,Gurung RB,Dhakal D,Sohng JK

更新日期：2014-10-01 00:00:00

abstract：:Developing the production of ectomycorrhizal (ECM) mushrooms in forest has become a challenge. Only a few ECM species are currently cultivable. Controlled mycorrhization practices offer promising advance to produce currently uncultivable ECM mushrooms. The persistence of the production of edible species, either cultiv...

journal_title：Applied microbiology and biotechnology

authors： Savoie JM,Largeteau ML

更新日期：2011-02-01 00:00:00

abstract：:The kinetics of growth, acid and solvent production in batch culture of Clostridium pasteurianum DSMZ 525 were examined in mixed or mono-substrate fermentations. In pH-uncontrolled batch cultures, the addition of butyric acid or glucose significantly enhanced n-butanol production and the ratio of butanol/1,3-propanedi...

journal_title：Applied microbiology and biotechnology

authors： Sabra W,Groeger C,Sharma PN,Zeng AP

更新日期：2014-05-01 00:00:00

abstract：:Although the de novo biosynthetic mechanism of 3-hydroxypropionic acid (3-HP) in glycerol-fermenting microorganisms is still unclear, the propanediol utilization protein (PduP) of Lactobacillus species has been suggested to be a key enzyme in this regard. To verify this hypothesis, a pduP gene from Lactobacillus reute...

journal_title：Applied microbiology and biotechnology

authors： Luo LH,Seo JW,Baek JO,Oh BR,Heo SY,Hong WK,Kim DH,Kim CH

更新日期：2011-02-01 00:00:00

abstract：:Wool and silk are major protein fiber materials used by the textile industry. Fiber protein structure-function relationships are briefly described here, and the major enzymatic processing routes for textiles and other novel applications are deeply reviewed. Fiber biomodification is described here with various classes ...

journal_title：Applied microbiology and biotechnology

authors： Fu J,Su J,Wang P,Yu Y,Wang Q,Cavaco-Paulo A

更新日期：2015-12-01 00:00:00

abstract：:To seek more information on function of two-component regulatory systems (TCSs) in Streptomyces coelicolor, a dozen TCS-knockout mutants were generated, and phenotype changes were determined. One TCS (SCO5403/5404)-deleted mutant with phenotype change was obtained. Here, we report the characterization of this novel TC...

journal_title：Applied microbiology and biotechnology

authors： Lu Y,Wang W,Shu D,Zhang W,Chen L,Qin Z,Yang S,Jiang W

更新日期：2007-12-01 00:00:00

abstract：:Corynebacterium glutamicum owns a citrate synthase and two methylcitrate synthases. Characterization of the isolated enzymes showed that the two methylcitrate synthases have comparable catalytic efficiency, k (cat)/K (m), as the citrate synthase with acetyl-CoA as substrate, although these enzymes are only synthesized...

journal_title：Applied microbiology and biotechnology

authors： Radmacher E,Eggeling L

更新日期：2007-09-01 00:00:00

abstract：:Quorum sensing (QS) plays a key role in activating bacterial functions through small molecules called autoinducers. In this study, the QS of Gram-negative bacteria in waste sewage sludge (WSS) was downregulated by adding the quorum quenching enzyme, AiiM lactonase, which cleaved the acyl-homoserine lactone (AHL) autoi...

journal_title：Applied microbiology and biotechnology

authors： Nguyen PDT,Mustapha NA,Kadokami K,Garcia-Contreras R,Wood TK,Maeda T

更新日期：2019-02-01 00:00:00