Abstract：
:This paper reports a novel integrated metabolic pathway of amino acid production through the utilization of phenol in Corynebacterium glutamicum. In the presence of 8.5 mM phenol, the level of glutamate and proline production increased up to 1.2- and 14.7-fold, respectively, compared to the control condition. In addition, their productivities increased 1.6- and 20-fold in the culture medium using phenol as the sole carbon source with 72 microM FeSO(4) as iron supplementation. Chromatin immunoprecipitation assay showed that the DNA-binding affinity of ArgR as a transcriptional repressor to the upstream of gltB and gdh gene was reduced significantly in the presence of 8.5 mM phenol. In addition, the DNA-binding affinity of ArgR to the upstream of gltB and gdh gene by iron supplementation was severely reduced, more than that under only 8.5 mM phenol. These results are consistent with those showing an increase in the mRNA levels of the two genes in the presence of 8.5 mM phenol and iron supplementation. Overall, iron enhances the biotransformation to glutamate and proline from phenol because of the regulated transcriptional levels. Furthermore, the biotransformation of phenol to glutamate and proline probably occurs through an interaction between ArgR and the gltB and gdh genes.
journal_name
Appl Microbiol Biotechnoljournal_title
Applied microbiology and biotechnologyauthors
Lee SY,Kim YH,Min Jdoi
10.1007/s00253-009-2206-2subject
Has Abstractpub_date
2010-01-01 00:00:00pages
713-20issue
3eissn
0175-7598issn
1432-0614journal_volume
85pub_type
杂志文章abstract：:Three different mechanisms can be envisaged that are used by fungi to solubilize coal: the production of alkaline substances, the extrusion of chelators and, of special interest in the scope of biotechnology, the action of enzymes. Whether these mechanisms are operating separately or in various combinations has not ye...
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