Optimisation of signal peptide for recombinant protein secretion in bacterial hosts.

abstract：:Mycobacterium tuberculosis is able to transition into a dormant state, causing a latent state of tuberculosis. Dormant mycobacteria acquire phenotypic resistance to all known antibacterial drugs; they are also able to maintain vitality in the host for decades and become active, causing the active form of the disease. ...

journal_title：Applied microbiology and biotechnology

authors： Shleeva MO,Savitsky AP,Nikitushkin VD,Solovyev ID,Kazachkina NI,Perevarov VV,Kaprelyants AS

更新日期：2019-12-01 00:00:00

abstract：:Significant decreases in the apparent viscosity of a bacterial biofilm suspension were measured following addition of sodium, potassium, magnesium, or calcium salts, whereas iron salts increased the viscosity. Electrostatic interactions contribute to biofilm cohesion and iron cations are potent crosslinkers of the bio...

journal_title：Applied microbiology and biotechnology

authors： Chen X,Stewart PS

更新日期：2002-09-01 00:00:00

abstract：:Arthrobacter sp. strain KI72 grows on a 6-aminohexanoate oligomer, which is a by-product of nylon-6 manufacturing, as a sole source of carbon and nitrogen. We cloned the two genes, nylD 1 and nylE 1 , responsible for 6-aminohexanoate metabolism on the basis of the draft genomic DNA sequence of strain KI72. We amplifie...

journal_title：Applied microbiology and biotechnology

authors： Takehara I,Fujii T,Tanimoto Y,Kato DI,Takeo M,Negoro S

更新日期：2018-01-01 00:00:00

abstract：:The 454 pyrosequencing technique was applied to evaluate microbial community composition in sediment and water samples collected from the river receiving effluents from a swine farm and a farmhouse restaurant, respectively. For each sample, 4,600 effective sequences were selected and used to do the bacterial diversity...

journal_title：Applied microbiology and biotechnology

authors： Lu XM,Lu PZ

更新日期：2014-09-01 00:00:00

abstract：:The microbes present in bioethanol production processes have been previously studied in laboratory-scale experiments, but there is a lack of information on full-scale industrial processes. In this study, the microbial communities of three industrial bioethanol production processes were characterized using several meth...

journal_title：Applied microbiology and biotechnology

authors： Aho VTE,Tolonen T,Haverinen J,Jaakkola M,Paulin L,Auvinen P,Laine MM

更新日期：2020-09-01 00:00:00

abstract：:Non-covalent interaction of alcohol dehydrogenase with polysaccharides was studied using three neutral and three anionic polysaccharides. The process of interaction of alcohol dehydrogenase with gum Arabic was optimized with respect to the ratio of enzyme to gum Arabic, pH, and molarity of buffer. Alcohol dehydrogenas...

journal_title：Applied microbiology and biotechnology

authors： Jadhav SB,Bankar SB,Granström T,Ojamo H,Singhal RS,Survase SA

更新日期：2014-01-01 00:00:00

abstract：:Nanotechnology is a promising field of interdisciplinary research. It opens up a wide array of opportunities in various fields like medicine, pharmaceuticals, electronics and agriculture. The potential uses and benefits of nanotechnology are enormous. These include insect pests management through the formulations of n...

journal_title：Applied microbiology and biotechnology

authors： Rai M,Ingle A

更新日期：2012-04-01 00:00:00

abstract：:River-bay system is a transitional zone connecting land and ocean and an important natural source for methane emission. Methanogens play important roles in the global greenhouse gas budget and carbon cycle since they produce methane. The abundance and community assemblage of methanogens in such a dynamic system are no...

journal_title：Applied microbiology and biotechnology

authors： Zhang CJ,Chen YL,Pan J,Wang YM,Li M

更新日期：2020-05-01 00:00:00

abstract：:Nitrite-dependent anaerobic methane oxidation (N-DAMO), which couples anaerobic methane oxidation and nitrite reduction, is a recently discovered bioprocess coupling microbial nitrogen and carbon cycles. The discovery of this microbial process challenges the traditional knowledge of global methane sinks and nitrogen l...

journal_title：Applied microbiology and biotechnology

authors： Wang J,Shen L,He Z,Hu J,Cai Z,Zheng P,Hu B

更新日期：2017-11-01 00:00:00

abstract：:The impact of dibenzofuran (DF) and dibenzo-p-dioxin (DD) on the changes in bacterial community structure and the transition of catabolic genes were studied using forest soil. The bacterial community structure of soil suspensions amended with 1 microg/g of either DF or DD was analyzed by 16S rRNA and functional gene s...

journal_title：Applied microbiology and biotechnology

authors： Kimura N,Kamagata Y

更新日期：2009-08-01 00:00:00

abstract：:This study has investigated the acclimation of ammonia-oxidizing communities (AOC) to low dissolved oxygen (DO) concentrations. Under controlled laboratory conditions, two sequencing batch reactors seeded with activated sludge from the same source were operated at high DO (near saturation) and low DO (0.1 mg O₂/L) con...

journal_title：Applied microbiology and biotechnology

authors： Arnaldos M,Kunkel SA,Stark BC,Pagilla KR

更新日期：2013-12-01 00:00:00

abstract：:Rhodococcus erythropolis cells contain a large set of enzymes that allow them to carry out an enormous number of bioconversions and degradations. Oxidations, dehydrogenations, epoxidations, hydrolysis, hydroxylations, dehalogenations and desulfurisations have been reported to be performed by R. erythropolis cells or e...

journal_title：Applied microbiology and biotechnology

authors： de Carvalho CC,da Fonseca MM

更新日期：2005-06-01 00:00:00

abstract：:Chitooligosaccharides have important application value in the fields of food and agriculture. Chitosanase can degrade chitosan to obtain chitooligosaccharides. The marine metagenome contains many genes related to the degradation of chitosan. However, it is difficult to mine valuable genes from large gene resources. Th...

journal_title：Applied microbiology and biotechnology

authors： Han Y,Guan F,Sun J,Wu N,Tian J

更新日期：2020-08-01 00:00:00

abstract：:The medicinal mushroom Agaricus blazei produced high amounts of laccase (up to 5,000 units l(-1)) in a complex, agitated liquid medium based on tomato juice, while only traces of the enzyme (<100 units l(-1)) were detected in synthetic glucose-based medium. Purification of the enzyme required three chromatographic ste...

journal_title：Applied microbiology and biotechnology

authors： Ullrich R,Huong le M,Dung NL,Hofrichter M

更新日期：2005-05-01 00:00:00

abstract：:The lack of a universal method to extract RNA from soil hinders the progress of studies related to nitrification in soil, which is an important step in the nitrogen cycle. It is particularly difficult to extract RNA from certain types of soils such as Andosols (volcanic ash soils), which is the dominant agricultural s...

journal_title：Applied microbiology and biotechnology

authors： Wang Y,Nagaoka K,Hayatsu M,Sakai Y,Tago K,Asakawa S,Fujii T

更新日期：2012-11-01 00:00:00

abstract：:Indigo is one of the oldest textile dyes and was originally prepared from plant material. Nowadays, indigo is chemically synthesized at a large scale to satisfy the demand for dyeing jeans. The current indigo production processes are based on fossil feedstocks; therefore, it is highly attractive to develop a more sust...

journal_title：Applied microbiology and biotechnology

authors： Fabara AN,Fraaije MW

更新日期：2020-02-01 00:00:00

abstract：:Xanthomonas campestris pv. campestris, the causal agent of black-rot disease of cruciferous plants, and an important industrial microbe, was able to express the Escherichia coli beta-glucuronidase reporter gene (uidA) when fused to the E. coli lactose operon promoter on a wide-host-range plasmid vector. The gene fusio...

journal_title：Applied microbiology and biotechnology

authors： Soby SD,Daniels MJ

更新日期：1996-12-01 00:00:00

abstract：:In this study, we aimed at determining the impact of naphthalene and different oxygen levels on a biofilm bacterial community originated from a petroleum hydrocarbon-contaminated groundwater. By using cultivation-dependent and cultivation-independent approaches, the enrichment, identification, and isolation of aerobic...

journal_title：Applied microbiology and biotechnology

authors： Benedek T,Szentgyörgyi F,Szabó I,Farkas M,Duran R,Kriszt B,Táncsics A

更新日期：2020-07-01 00:00:00

abstract：:Baicalin (baicalein 7-O-β-D-glucuronide) is one of the major flavonoid glucuronides found in traditional herbal medicines. Because its aglycone, baicalein, is absorbed more quickly and shows more effective properties than baicalin, the conversion of baicalin into baicalein by β-glucuronidase (GUS) has drawn the attent...

journal_title：Applied microbiology and biotechnology

authors： Sakurama H,Kishino S,Uchibori Y,Yonejima Y,Ashida H,Kita K,Takahashi S,Ogawa J

更新日期：2014-05-01 00:00:00

abstract：:With their ability to lyse Gram-positive bacteria, phage lytic enzymes (or lysins) have received a great deal of attention as novel anti-infective agents. The number of known genes encoding these peptidoglycan hydrolases has increased markedly in recent years, due in large part to advances in DNA sequencing technology...

journal_title：Applied microbiology and biotechnology

authors： Schmitz JE,Ossiprandi MC,Rumah KR,Fischetti VA

更新日期：2011-03-01 00:00:00

abstract：:Seven fractions rich in endoglucanase activity were separated from the extracellular cellulase system of the anaerobic rumen fungus Neocallimastix frontalis. The fractions (ES1, ES3, ES2U1, ES2U2, ES2U4, ES2U3C1 and ES2U3C2) were separated from each other and from a fraction that could solubilize crystalline cellulose...

journal_title：Applied microbiology and biotechnology

authors： Wood TM,Wilson CA,McCrae SI

更新日期：1995-12-01 00:00:00

abstract：:Glycerol formation is vital for reoxidation of nicotinamide adenine dinucleotide (reduced form; NADH) under anaerobic conditions and for the hyperosmotic stress response in the yeast Saccharomyces cerevisiae. However, relatively few studies have been made on hyperosmotic stress under anaerobic conditions. To study the...

journal_title：Applied microbiology and biotechnology

authors： Modig T,Granath K,Adler L,Lidén G

更新日期：2007-05-01 00:00:00

abstract：:A fluidized-bed reactor (FBR) was used to enrich an aerobic chlorophenol-degrading microbial culture. Long-term continuous-flow operation with low effluent concentrations selected oligotrophic microorganisms producing good-quality effluent for pentachlorophenol(PCP)-contaminated water. PCP biodegradation kinetics was ...

journal_title：Applied microbiology and biotechnology

authors： Melin ES,Ferguson JF,Puhakka JA

更新日期：1997-06-01 00:00:00

abstract：:Heterorhabditis bacteriophora is used in biological control of soil-borne insect pests in horticulture and turf. Mass production is carried out in monoxenic liquid cultures pre-incubated with the symbiont of the nematodes, the bacterium Photorhabdus luminescens, before nematode dauer juveniles (DJ) are inoculated. As ...

journal_title：Applied microbiology and biotechnology

authors： Johnigk SA,Ecke F,Poehling M,Ehlers RU

更新日期：2004-06-01 00:00:00

abstract：:Magnetic carbon nanotubes (MCNTs) with necklace-like nanostructures was prepared via hydrothermal method, and hyperbranched poly(amidoamine) (PAMAM) was grafted on the surface of MCNTs on the basis of the Michael addition of methyl acrylate and the amidation of the resulting ester with a large excess of ethylenediamin...

journal_title：Applied microbiology and biotechnology

authors： Zhao G,Li Y,Wang J,Zhu H

更新日期：2011-08-01 00:00:00

abstract：:The effects of methyl jasmonate (MJ) elicitation on the cell growth and accumulation of ginsenoside in 5-l bioreactor suspension cultures of Panax ginseng were investigated. Ginsenoside accumulation was enhanced by elicitation by MJ (in the range 50-400 microM); however, fresh weight, dry weight and growth ratio of th...

journal_title：Applied microbiology and biotechnology

authors： Thanh NT,Murthy HN,Yu KW,Hahn EJ,Paek KY

更新日期：2005-04-01 00:00:00

abstract：:Brown rot wood-degrading fungi distinctly modify lignocellulose and completely hydrolyze polysaccharides (saccharification), typically without secreting an exo-acting glucanase and without removing lignin. Although each step of this two-step approach evolved within the same organism, it is unknown if the early lignoce...

journal_title：Applied microbiology and biotechnology

authors： Schilling JS,Tewalt JP,Duncan SM

更新日期：2009-09-01 00:00:00

abstract：:The paper describes the synthesis of the phosphorylcholine-binding miniantibody McPC603scFvDhl x in cell-wall-less L-form strains of Escherichia coli and Proteus mirabilis. Cells of these strains were transformed with the plasmid pACK02scKan, carrying the miniantibody (miniAb) coding sequence under the control of the ...

journal_title：Applied microbiology and biotechnology

authors： Kujau MJ,Hoischen C,Riesenberg D,Gumpert J

更新日期：1998-01-01 00:00:00

abstract：:Mutagenesis and selection were applied to a strain of Penicillium echinulatum by treating conidia with hydrogen peroxide or 1,2,7,8-diepoxyoctane and then by incubating the conidia for 48 h in broth containing microcrystalline cellulose washed in 0.5% (w/v) aqueous 2-deoxyglucose before plating them onto cellulose aga...

journal_title：Applied microbiology and biotechnology

authors： Dillon AJ,Zorgi C,Camassola M,Henriques JA

更新日期：2006-05-01 00:00:00

abstract：:Neuritin (also known as candidate plasticity gene 15 (cpg15)) is a neurotrophic factor that was recently discovered in a screen aimed at identifying genes involved in activity-dependent synaptic plasticity. Neuritin plays multiple roles in both neural development (Chen et al. Zhonghua Yan Ke Za Zhi 46:978-983 2010; Co...

journal_title：Applied microbiology and biotechnology

authors： Zhang Y,Zhang S,Xian L,Tang J,Zhu J,Cui L,Li S,Yang L,Huang J

更新日期：2015-10-01 00:00:00