Expression and purification of recombinant human neuritin from Pichia pastoris and a partial analysis of its neurobiological activity in vitro.

abstract：:Silver nanoparticles (AgNPs) have received tremendous attention due to their significant antimicrobial properties. Large numbers of reports are available on the physical, chemical, and biological syntheses of colloidal AgNPs. Since there is a great need to develop ecofriendly and sustainable methods, biological system...

journal_title：Applied microbiology and biotechnology

authors： Singh R,Shedbalkar UU,Wadhwani SA,Chopade BA

更新日期：2015-06-01 00:00:00

abstract：:To measure the toxicity experienced by superoxide-generating compounds, two plasmids were constructed in which the superoxide-inducible fpr and zwf promoters from Escherichia coli were fused to promoterless Vibrio fischeri luxCDABE operon present in plasmid pUCD615. The bioluminescent response of E. coli harboring the...

journal_title：Applied microbiology and biotechnology

authors： Niazi JH,Kim BC,Gu MB

更新日期：2007-04-01 00:00:00

abstract：:Based on natural selection and the survival of the fittest by evolutionary adaption, a smart high-throughput system was developed to select active haloalkane dehalogenase variants from a large mutant library. Only active enzyme variants can hydrolyse toxic halogenated alkanes to promote growth, whereas inactive mutant...

journal_title：Applied microbiology and biotechnology

authors： Fibinger MP,Davids T,Böttcher D,Bornscheuer UT

更新日期：2015-11-01 00:00:00

abstract：:Antimicrobial dendrimeric peptides (AMDP) are a relatively new class of agents displaying repetitive functional groups on a branched core. Previously, we have investigated the length requirement for antimicrobial activity of peptides consisting of repeated arginine (R) and tryptophan (W) side chains and found that eve...

journal_title：Applied microbiology and biotechnology

authors： Bahar AA,Liu Z,Totsingan F,Buitrago C,Kallenbach N,Ren D

更新日期：2015-10-01 00:00:00

abstract：:This paper reports the in vivo expression of the synthetic transposase gene tnp(a) from a hyperactive Tn5 tnp gene mutant in Streptomyces coelicolor. Using the synthetic tnp(a) gene adapted for Streptomyces codon usage, we showed random insertion of the transposon into the Streptomycetes genome. The insertion frequenc...

journal_title：Applied microbiology and biotechnology

authors： Petzke L,Luzhetskyy A

更新日期：2009-07-01 00:00:00

abstract：:Biotin (Vitamin B7) is a critical enzyme co-factor in metabolic pathways important for bacterial survival. Biotin is obtained either from the environment or by de novo synthesis, with some bacteria capable of both. In certain species, the bifunctional protein BirA plays a key role in biotin homeostasis as it regulates...

journal_title：Applied microbiology and biotechnology

authors： Satiaputra J,Eijkelkamp BA,McDevitt CA,Shearwin KE,Booker GW,Polyak SW

更新日期：2018-04-01 00:00:00

abstract：:Clostridium acetobutylicum is an important industrial microorganism for n-butanol bioproduction, and its transcription factor AbrB0310 regulates various important cellular processes. However, the roles of two abrB homologues, abrB1941 and abrB3647, have not been determined because they appear inactive during transcrip...

journal_title：Applied microbiology and biotechnology

authors： Xue Q,Yang Y,Chen J,Chen L,Yang S,Jiang W,Gu Y

更新日期：2016-11-01 00:00:00

abstract：:The interest in industrial biotechnology and its importance opens up challenging possibilities of research in this area. Surfactants have long been among the most versatile of process chemicals. Their market is extremely competitive and manufacturers will have to expand their arsenal to develop products for the year 2...

journal_title：Applied microbiology and biotechnology

authors： Cameotra SS,Makkar RS

更新日期：1998-11-01 00:00:00

abstract：:Aspergillus niger var. awamori was previously transformed with a vector designed to express a fused glucoamylase-prochymosin gene and bearing the Neurospora crassa pyr4 gene as a selectable marker. Mutant strains that overproduced the glucoamylase-prochymosin fusion protein were derived from one of the transformants. ...

journal_title：Applied microbiology and biotechnology

authors： Ward M,Wilson LJ,Kodama KH

更新日期：1993-08-01 00:00:00

abstract：:To develop a high efficiency Candida antarctica lipase B (CALB) yeast display system, we linked two CALB genes fused with Sacchromyces cerevisiae cell wall protein genes, the Sed1 and the 3'-terminal half of Sag1, separately by a 2A peptide of foot-and-mouth disease virus (FMDV) in a single open reading frame. The CAL...

journal_title：Applied microbiology and biotechnology

authors： Sun YF,Lin Y,Zhang JH,Zheng SP,Ye YR,Liang XX,Han SY

更新日期：2012-12-01 00:00:00

abstract：:Enzymatic production of biodiesel by transesterification of triglycerides and alcohol, catalyzed by lipases, offers an environmentally friendly and efficient alternative to the chemically catalyzed process while using low-grade feedstocks. Methanol is utilized frequently as the alcohol in the reaction due to its react...

journal_title：Applied microbiology and biotechnology

authors： Dror A,Kanteev M,Kagan I,Gihaz S,Shahar A,Fishman A

更新日期：2015-11-01 00:00:00

abstract：:Aldolases are a specific group of lyases that catalyze the reversible stereoselective addition of a donor compound (nucleophile) onto an acceptor compound (electrophile). Whereas most aldolases are specific for their donor compound in the aldolization reaction, they often tolerate a wide range of aldehydes as acceptor...

journal_title：Applied microbiology and biotechnology

authors： Samland AK,Sprenger GA

更新日期：2006-07-01 00:00:00

abstract：:A 6-kDa antifungal peptide with inhibitory activity on mycelial growth in Fusarium oxysporum, Mycosphaerella arachidicola, and Physalospora piricola was isolated from baby lima beans. The peptide suppressed growth in M. arachidicola with an IC(50) of 0.87 muM and inhibited activity of HIV-1 reverse transcriptase with ...

journal_title：Applied microbiology and biotechnology

authors： Wang HX,Ng TB

更新日期：2006-12-01 00:00:00

abstract：:Myo-inositol is important for Streptomyces growth and morphological differentiation. Genomic sequence analysis revealed a myo-inositol catabolic gene cluster in Streptomyces coelicolor. Disruption of the corresponding genes in this cluster abolished the bacterial growth on myo-inositol as a single carbon source. The t...

journal_title：Applied microbiology and biotechnology

authors： Yu L,Li S,Gao W,Pan Y,Tan H,Liu G

更新日期：2015-04-01 00:00:00

abstract：:A cDNA encoding a bifunctional acetylxylan esterase/xylanase, XynS20E, was cloned from the ruminal fungus Neocallimastix patriciarum. A putative conserved domain of carbohydrate esterase family 1 was observed at the N-terminus and a putative conserved domain of glycosyl hydrolase family 11 was detected at the C-termin...

journal_title：Applied microbiology and biotechnology

authors： Pai CK,Wu ZY,Chen MJ,Zeng YF,Chen JW,Duan CH,Li ML,Liu JR

更新日期：2010-02-01 00:00:00

abstract：:Human epidermal growth factor receptors (EGFR) are an important target in drug discovery in terms of both protein-small-molecule interactions and protein-protein interactions. In this work, the isolation of a stable soluble protein of the tyrosine kinase domain of EGFR in Escherichia coli expression has been accomplis...

journal_title：Applied microbiology and biotechnology

authors： Seetaha S,Ratanabanyong S,Choowongkomon K

更新日期：2019-10-01 00:00:00

abstract：:Diosgenin transformation was studied in Streptomyces virginiae IBL-14, a soil-dwelling bacterium with diosgenin-degrading capacity. All of the derivatives isolated were identified as 4-ene-3-keto steroids. We cloned ChoL, a fragment of a cholesterol oxidase from S. virginiae IBL-14, and used gene-disruption techniques...

journal_title：Applied microbiology and biotechnology

authors： Li B,Wang W,Wang FQ,Wei DZ

更新日期：2010-02-01 00:00:00

abstract：:Reduction of azo dye methyl red coupled with aerobic respiration by growing cultures of Klebsiella oxytoca GS-4-08 was investigated. In liquid media containing dye and 0.6 % glucose in a mineral salts base, 100 mg l(-1) of the dye are completely removed in 3 h under shaking conditions. The dye cannot be aerobically de...

journal_title：Applied microbiology and biotechnology

authors： Yu L,Zhang XY,Xie T,Hu JM,Wang S,Li WW

更新日期：2015-03-01 00:00:00

abstract：:Phenotypic plasticity is the capacity to change the phenotype in response to different environments without alteration of the genotype. Despite sufficient evidence that microorganisms have a major role in the fitness and sickness of eukaryotes, there has been little research regarding microbial phenotypic plasticity. ...

journal_title：Applied microbiology and biotechnology

authors： Zheng X,Bai J,Ye M,Liu Y,Jin Y,He X

更新日期：2020-06-01 00:00:00

abstract：:Alcoholic fermentation of grape must is a complex process, involving several yeast genera and species. The early stages in fermentation are dominated by non-Saccharomyces yeasts that are gradually replaced by the Saccharomyces cerevisiae species, which takes over the fermentation. Quantitative studies have reported th...

journal_title：Applied microbiology and biotechnology

authors： Renault PE,Albertin W,Bely M

更新日期：2013-05-01 00:00:00

abstract：:Respiration measurement in shake flasks is introduced as a new method to characterize the metabolic activity of microorganisms during and after stress exposure. The major advantage of the new method is the possibility to determine the metabolic activity independent of manual sampling without the necessity to change th...

journal_title：Applied microbiology and biotechnology

authors： Seletzky JM,Noack U,Fricke J,Hahn S,Büchs J

更新日期：2006-10-01 00:00:00

abstract：:Variable domains of heavy chains of camelid heavy-chain antibodies (VHHs) are known as nanobodies. Nanobodies are approximately 15 kDa in size with high affinity to their antigens. They can be easily manipulated and produced in microorganisms. In this study, an alpaca was immunized with purified green fluorescence pro...

journal_title：Applied microbiology and biotechnology

authors： Fang Z,Cao D,Qiu J

更新日期：2020-06-01 00:00:00

abstract：:L-cysteine, a valuable sulfur-containing amino acid, has been widely used in food, agriculture, and pharmaceutical industries. Due to the toxicity and complex regulation of L-cysteine, no efficient cell factory has yet been achieved for L-cysteine industrial production. In this study, the food-grade microorganism Cory...

journal_title：Applied microbiology and biotechnology

authors： Wei L,Wang H,Xu N,Zhou W,Ju J,Liu J,Ma Y

更新日期：2019-02-01 00:00:00

abstract：:Glucosylglycerols (GGs) are known as compatible solutes accumulated by some bacteria including cyanobacteria as well as higher plants for their adaptations to salt or desiccation stresses. Since being identified in Japanese sake, their physiological effects and potential applications on human health cares have been ex...

journal_title：Applied microbiology and biotechnology

authors： Tan X,Luo Q,Lu X

更新日期：2016-07-01 00:00:00

abstract：:Medicinal plants of the Dendrobium genus are highly prized, and hence, methodologies have been developed to authenticate Dendrobium drugs from its adulterants. Many bioactive constituents of Dendrobium species have been identified. The macromolecules included lectins; the enzymes chalcone synthase, sucrose synthase, a...

journal_title：Applied microbiology and biotechnology

authors： Ng TB,Liu J,Wong JH,Ye X,Wing Sze SC,Tong Y,Zhang KY

更新日期：2012-03-01 00:00:00

abstract：:We engineered efficient 2,3-butanediol (23BD) production from cellobiose using Bacillus subtilis. First, we found that B. subtilis harboring an empty vector could produce 23BD from cellobiose. However, productivity using cellobiose as a carbon source was lower than that when using glucose. This lower productivity was ...

journal_title：Applied microbiology and biotechnology

authors： Tanimura K,Takashima S,Matsumoto T,Tanaka T,Kondo A

更新日期：2016-07-01 00:00:00

abstract：:Cultures of Umbelopsis ramanniana (=Mucor ramannianus) were grown in fluid Sabouraud medium for 3 days, dosed with 0.23 mM benzo[f]quinoline, benzo[h]quinoline, or phenanthridine (benzo[c]quinoline), and incubated for another 18 days. Cultures were extracted and metabolites (66-75% of the UV absorbance) were separated...

journal_title：Applied microbiology and biotechnology

authors： Sutherland JB,Cross EL,Heinze TM,Freeman JP,Moody JD

更新日期：2005-05-01 00:00:00

abstract：:The first two genes of the threonine pathway, ask and asd, were cloned and sequenced from the aminoethoxyvinylglycine-producing Streptomyces sp. NRRL 5331. The two genes are organized in a bicistronic operon. ask, encoding the apartokinase (ASK), is located upstream from asd. The presence of a ribosome-binding site wi...

journal_title：Applied microbiology and biotechnology

authors： Cuadrado Y,Fernández M,Recio E,Aparicio JF,Martín JF

更新日期：2004-04-01 00:00:00

abstract：:Bacterial cells are protected by an exoskeleton, the stabilizing and shape-maintaining cell wall, consisting of the complex macromolecule peptidoglycan. In view of its function, it could be assumed that the cell wall is a static structure. In truth, however, it is steadily broken down by peptidoglycan-cleaving enzymes...

journal_title：Applied microbiology and biotechnology

authors： Reith J,Mayer C

更新日期：2011-10-01 00:00:00

abstract：:To understand the effect of daily bioaugmentation in full-scale grease interceptors (GIs), we compared the microbial communities occurring in two full-scale GIs during bioaugmented and non-bioaugmented cycles. The changes in microbial communities were determined using terminal restriction fragment length polymorphism ...

journal_title：Applied microbiology and biotechnology

authors： He X,So MJ,de Los Reyes FL 3rd

更新日期：2016-08-01 00:00:00