Molecular cloning and characterization of a bifunctional xylanolytic enzyme from Neocallimastix patriciarum.

abstract：:Orange peel waste (OPW), the primary byproduct of the juice extraction process, is annually generated in massive amounts (21 Mton), and its aqueous extraction in biorefining operations yields a liquid fraction, referred to as orange peel extract (OPE). Although OPE contains significant amounts of easily assimilable ca...

journal_title：Applied microbiology and biotechnology

authors： Carota E,Petruccioli M,D'Annibale A,Gallo AM,Crognale S

更新日期：2020-05-01 00:00:00

abstract：:A novel system was developed for efficient transformation of the fungal biocontrol agent Beauveria bassiana. Competent blastospores were prepared and stored in LiAc- and glycerol-inclusive suspension at -76 degrees C for sequential use in transformation. The system was successfully applied to integrating phosphinothri...

journal_title：Applied microbiology and biotechnology

authors： Ying SH,Feng MG

更新日期：2006-08-01 00:00:00

abstract：:Tocopherols, collectively known as vitamin E, are lipophilic antioxidants, which are synthesized only by photosynthetic organisms. Due to their enormous potential to protect cells from oxidative damage, tocopherols are used, e.g., as nutraceuticals and additives in pharmaceuticals. The most biologically active form of...

journal_title：Applied microbiology and biotechnology

authors： Geipel K,Song X,Socher ML,Kümmritz S,Püschel J,Bley T,Ludwig-Müller J,Steingroewer J

更新日期：2014-03-01 00:00:00

abstract：:Transaminase enzymes (TAms) are becoming increasingly valuable in the chemist's toolbox as a biocatalytic route to chiral amines. Despite high profile successes, the lack of (R)-selective TAms and robustness under harsh industrial conditions continue to prove problematic. Herein, we report the isolation of the first h...

journal_title：Applied microbiology and biotechnology

authors： Kelly SA,Magill DJ,Megaw J,Skvortsov T,Allers T,McGrath JW,Allen CCR,Moody TS,Gilmore BF

更新日期：2019-07-01 00:00:00

abstract：:Gluconic acid secretion mediated by the direct oxidation of glucose by pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenase (GDH) is responsible for mineral phosphate solubilization in Gram-negative bacteria. Herbaspirillum seropedicae Z67 (ATCC 35892) genome encodes GDH apoprotein but lacks genes for the bi...

journal_title：Applied microbiology and biotechnology

authors： Wagh J,Shah S,Bhandari P,Archana G,Kumar GN

更新日期：2014-06-01 00:00:00

abstract：:The effects of methyl jasmonate (MJ) elicitation on the cell growth and accumulation of ginsenoside in 5-l bioreactor suspension cultures of Panax ginseng were investigated. Ginsenoside accumulation was enhanced by elicitation by MJ (in the range 50-400 microM); however, fresh weight, dry weight and growth ratio of th...

journal_title：Applied microbiology and biotechnology

authors： Thanh NT,Murthy HN,Yu KW,Hahn EJ,Paek KY

更新日期：2005-04-01 00:00:00

abstract：:Zygosaccharomyces rouxii plays important roles in the brewing process of fermented foods such as soy sauce, where salt stress is a frequently encountered condition. In this study, effect of heat preadaptation on salt tolerance of Z. rouxii and the protective mechanisms underlying heat preadaptation were investigated b...

journal_title：Applied microbiology and biotechnology

authors： Wang D,Zhang M,Huang J,Zhou R,Jin Y,Zhao D,Zheng J,Wu C

更新日期：2021-01-01 00:00:00

abstract：:The Vitreoscilla hemoglobin (VHb) gene (vgb) was integrated into the chromosome of Bacillus thuringiensis BMB171 using integrative vector pEG491. The production of VHb was confirmed by CO-difference spectra analysis. Fermentation experiments results showed that with the production of VHb, the critical oxygen concentra...

journal_title：Applied microbiology and biotechnology

authors： Liang F,Shouwen C,Ming S,Ziniu Y

更新日期：2007-02-01 00:00:00

abstract：:D-allose has attracted a great deal of attention in recent years due to its many pharmaceutical activities, which include anti-cancer, anti-tumor, anti-inflammatory, anti-oxidative, anti-hypertensive, cryoprotective, and immunosuppressant activities. D-allose has been produced from D-psicose using D-allose-producing e...

journal_title：Applied microbiology and biotechnology

authors： Lim YR,Oh DK

更新日期：2011-07-01 00:00:00

abstract：:As an alternative class of antimicrobial agents used to overcome drug-resistant infections, antimicrobial peptides (AMPs) have recently gained significant attention. In this study, we designed an improved antimicrobial peptide, K1K8, based on the molecular template of Hp1404. Compared to the wild-type Hp1404, K1K8 sho...

journal_title：Applied microbiology and biotechnology

authors： Li Z,Liu G,Meng L,Yu W,Xu X,Li W,Wu Y,Cao Z

更新日期：2016-06-01 00:00:00

abstract：:Evolvulus alsinoides L. is used for preparation of 'Shankhapushpi', an important popular ayurvedic drug that contributes considerably to the improvement of memory power. The improvement is attributed to the presence of furanocoumarin scopoletin, a metabolite with a wide range of biological activities. This report desc...

journal_title：Applied microbiology and biotechnology

authors： Naikawadi VB,Ahire ML,Lahiri A,Nikam TD

更新日期：2016-04-01 00:00:00

abstract：:Several studies support the use of probiotics for the treatment of minor gastrointestinal problems in infants. Positive effects on newborn colics have been evidenced after administration of Lactobacillus strains, whereas no studies have been reported regarding the use of bifidobacteria for this purpose. This work was ...

journal_title：Applied microbiology and biotechnology

authors： Aloisio I,Santini C,Biavati B,Dinelli G,Cencič A,Chingwaru W,Mogna L,Di Gioia D

更新日期：2012-12-01 00:00:00

abstract：:The genes encoding two conjugated polyketone reductases (CPR-C1, CPR-C2) of Candida parapsilosis IFO 0708 were cloned and sequenced. The genes encoded a total of 304 and 307 amino acid residues for CPR-C1 and CPR-C2, respectively. The deduced amino acid sequences of the two enzymes showed high similarity to each other...

journal_title：Applied microbiology and biotechnology

authors： Kataoka M,Delacruz-Hidalgo AR,Akond MA,Sakuradani E,Kita K,Shimizu S

更新日期：2004-04-01 00:00:00

abstract：:L-tryptophan (L-trp) is a biosynthetic precursor of various bioactive components with pharmaceutical interest. The development of an efficient L-trp production strain using targeted molecular engineering approaches is challenging due to the requirement of several precursors and the complex regulations of the pathways ...

journal_title：Applied microbiology and biotechnology

authors： Chen L,Zeng AP

更新日期：2017-01-01 00:00:00

abstract：:Residual biomass, produced by the thermophilic fungus, Talaromyces emersonii CBS 814.70, following growth on glucose-containing media, was examined for its ability to take up uranium from aqueous solution. It was found that the biomass had a relatively high observed biosorption capacity for the uranium (280 mg/g dry w...

journal_title：Applied microbiology and biotechnology

authors： Bengtsson L,Johansson B,Hackett TJ,McHale L,McHale AP

更新日期：1995-01-01 00:00:00

abstract：:The goal of modern taxonomy is to understand the relationships of living organisms in terms of evolutionary descent. Thereby, the relationships between living organisms are understood in terms of nested clades--every time a speciation event takes place, two new clades are produced. Life comprises three domains of livi...

journal_title：Applied microbiology and biotechnology

authors： Voigt K,Kirk PM

更新日期：2011-04-01 00:00:00

abstract：:Ammonia was observed as a potential significant factor to manipulate the abundance and activity of ammonia-oxidizing microorganisms (AOMs) in water environments. For the first time, this study confirmed this phenomenon by laboratory cultivation. In a series of estuarine sediment-coastal water microcosms, we investigat...

journal_title：Applied microbiology and biotechnology

authors： Zhang Y,Chen L,Dai T,Sun R,Wen D

更新日期：2015-08-01 00:00:00

abstract：:Traditional temperature-sensitive systems use either heat shock (40-42 °C) or cold shock (15-23 °C) to induce gene expression at temperatures that are not the optimal temperature for host cell growth (37 °C). This impacts the overall productivity and yield by disturbing cell growth and cellular metabolism. Here, we ha...

journal_title：Applied microbiology and biotechnology

authors： Jiang X,Zhang H,Yang J,Liu M,Feng H,Liu X,Cao Y,Feng D,Xian M

更新日期：2013-06-01 00:00:00

abstract：:To investigate the effect of fungal elicitors on biosynthesis of natamycin in the cultures of Streptomyces natalensis HW-2, the biomass and filtrate of the broth from Aspergillus niger AS 3.6472, Penicillium chrysogenum AS 3.5163, A. oryzae AS 3.2068, and Saccharomyces cerevisiae AS 2.2081 were used to induce natamyci...

journal_title：Applied microbiology and biotechnology

authors： Wang D,Yuan J,Gu S,Shi Q

更新日期：2013-06-01 00:00:00

abstract：:The sequence of gene xynB encoding xylanase B from Paenibacillus sp. BP-23 was determined. It revealed an open reading frame of 999 nucleotides encoding a protein of 38,561 Da. The deduced amino acid sequence of xylanase B shows that the N-terminal region of the enzyme lacks the features of a signal peptide. When the ...

journal_title：Applied microbiology and biotechnology

authors： Gallardo O,Diaz P,Pastor FI

更新日期：2003-05-01 00:00:00

abstract：:Cupriavidus necator JMP134 utilizes meta-nitrophenol (MNP) as a sole source of carbon, nitrogen, and energy. The metabolic reconstruction of MNP degradation performed in silico suggested that the mnp cluster might have played important roles in MNP degradation. In order to experimentally confirm the prediction, we hav...

journal_title：Applied microbiology and biotechnology

authors： Yin Y,Xiao Y,Liu HZ,Hao F,Rayner S,Tang H,Zhou NY

更新日期：2010-08-01 00:00:00

abstract：:A cost-effective conversion of lignocellulosic biomass into bioethanol requires that the xylose released from the hemicellulose fraction (20-40% of biomass) can be fermented. Baker's yeast, Saccharomyces cerevisiae, efficiently ferments glucose but it lacks the ability to ferment xylose. Xylose-fermenting yeast such a...

journal_title：Applied microbiology and biotechnology

authors： Hou X

更新日期：2012-04-01 00:00:00

abstract：:Rhamnolipids produced by Pseudomonas aeruginosa are biosurfactants with a high biotechnological potential, but their extensive commercialization is limited by the potential virulence of P. aeruginosa and by restrictions in producing these surfactants in heterologous hosts. In this work, we report the characterization ...

journal_title：Applied microbiology and biotechnology

authors： Grosso-Becerra MV,González-Valdez A,Granados-Martínez MJ,Morales E,Servín-González L,Méndez JL,Delgado G,Morales-Espinosa R,Ponce-Soto GY,Cocotl-Yañez M,Soberón-Chávez G

更新日期：2016-12-01 00:00:00

abstract：:Nervonic acid (NA) is a major very long-chain monounsaturated fatty acid found in the white matter of mammalian brains, which plays a critical role in the treatment of psychotic disorders and neurological development. In the nature, NA has been synthesized by a handful plants, fungi, and microalgae. Although the metab...

journal_title：Applied microbiology and biotechnology

authors： Fan Y,Meng HM,Hu GR,Li FL

更新日期：2018-04-01 00:00:00

abstract：:We report the successful high-yield expression of Candida utilis uricase in Escherichia coli and the establishment of an efficient three-step protein purification protocol. The purity of the recombinant protein, which was confirmed to be C. utilis uricase by sodium dodecyl sulfate-polyacrylamide gel electrophoresis an...

journal_title：Applied microbiology and biotechnology

authors： Liu X,Wen M,Li J,Zhai F,Ruan J,Zhang L,Li S

更新日期：2011-11-01 00:00:00

abstract：:A moderately halophilic alkalitolerant Bacillus sp. Strain TSCVKK, with an ability to produce extracellular halophilic, alkalitolerant, surfactant, and detergent-stable alpha-amylase was isolated from soil samples obtained from a salt-manufacturing industry in Chennai. The culture conditions for higher amylase product...

journal_title：Applied microbiology and biotechnology

authors： Kiran KK,Chandra TS

更新日期：2008-01-01 00:00:00

abstract：:Escherichia coli BL21, expressing a quintuple mutant of P450(BM-3), oxyfunctionalizes alpha-pinene in an NADPH-dependent reaction to alpha-pinene oxide, verbenol, and myrtenol. We optimized the whole-cell biocatalyst by integrating a recombinant intracellular NADPH regeneration system through co-expression of a glucos...

journal_title：Applied microbiology and biotechnology

authors： Schewe H,Kaup BA,Schrader J

更新日期：2008-02-01 00:00:00

abstract：:Acid tolerance and aroma profile are crucial factors for wine production in Saccharomyces cerevisiae. However, most wine yeasts to date fail to endure low-pH environments, therefore resulting in problems such as lengthened fermentation and poor flavor during acidic fruit wine production. In the present study, we estab...

journal_title：Applied microbiology and biotechnology

authors： Tian T,Wu D,Ng CT,Yang H,Sun J,Liu J,Lu J

更新日期：2020-04-01 00:00:00

abstract：:Biotin (Vitamin B7) is a critical enzyme co-factor in metabolic pathways important for bacterial survival. Biotin is obtained either from the environment or by de novo synthesis, with some bacteria capable of both. In certain species, the bifunctional protein BirA plays a key role in biotin homeostasis as it regulates...

journal_title：Applied microbiology and biotechnology

authors： Satiaputra J,Eijkelkamp BA,McDevitt CA,Shearwin KE,Booker GW,Polyak SW

更新日期：2018-04-01 00:00:00

abstract：:Three cytosolic NADPH-dependent flavin-associated proteins (Gox2107, Gox0502, and Gox2684) from Gluconobacter oxydans 621H were overproduced in Escherichia coli, and the recombinant enzymes were purified and characterized. Apparent native molecular masses of 65.2, 78.2, and 78.4 kDa were observed for Gox2107, Gox0502,...

journal_title：Applied microbiology and biotechnology

authors： Schweiger P,Gross H,Wesener S,Deppenmeier U

更新日期：2008-10-01 00:00:00