Gene cloning and overexpression of two conjugated polyketone reductases, novel aldo-keto reductase family enzymes, of Candida parapsilosis.

abstract：:The carbon and nitrogen sources most suitable for L-asparaginase production by Enterobacter aerogenes were selected and their concentrations optimized in shake-flask cultures. Sodium citrate (1.0%) and diammonium hydrogen phosphate (0.16%) proved to be the best sources of carbon and nitrogen, respectively. Nitrogen ca...

journal_title：Applied microbiology and biotechnology

authors： Mukherjee J,Majumdar S,Scheper T

更新日期：2000-02-01 00:00:00

abstract：:Ideal immunogenicity in antigens is a prerequisite to eliciting a sufficiently strong immune and memory response via either DNA or protein vaccines. To improve immunogenicity, efforts have focused on high-level expression of target proteins and on maintaining their natural conformations. In the present work, two trime...

journal_title：Applied microbiology and biotechnology

authors： Wang JY,Song WT,Li Y,Chen WJ,Yang D,Zhong GC,Zhou HZ,Ren CY,Yu HT,Ling H

更新日期：2011-08-01 00:00:00

abstract：:The pyrene-degrading Mycobacterium strain AP1 was found to utilize fluoranthene as a sole source of carbon and energy. Identification of metabolites formed from fluoranthene (by growing cells and washed-cell suspensions), the kinetics of metabolite accumulation, and metabolite-feeding studies all indicated that strain...

journal_title：Applied microbiology and biotechnology

authors： López Z,Vila J,Minguillón C,Grifoll M

更新日期：2006-05-01 00:00:00

abstract：:Functional genomics of filamentous fungi has gradually uncovered gene information for constructing 'cell factories' and controlling pathogens. Available gene manipulation methods of filamentous fungi include random integration methods, gene targeting technology, gene editing with artificial nucleases and RNA technolog...

journal_title：Applied microbiology and biotechnology

authors： Wang S,Chen H,Tang X,Zhang H,Chen W,Chen YQ

更新日期：2017-11-01 00:00:00

abstract：:Enzymatic production of biodiesel by transesterification of triglycerides and alcohol, catalyzed by lipases, offers an environmentally friendly and efficient alternative to the chemically catalyzed process while using low-grade feedstocks. Methanol is utilized frequently as the alcohol in the reaction due to its react...

journal_title：Applied microbiology and biotechnology

authors： Dror A,Kanteev M,Kagan I,Gihaz S,Shahar A,Fishman A

更新日期：2015-11-01 00:00:00

abstract：:A system for the controlled expression of a foreign gene in cultured tobacco cells (Nicotiana tabacum, BY2) by temperature shift was constructed. A 925-base-pair (bp) DNA fragment containing the 5'-flanking region of a low-molecular-mass heat-shock protein gene (HSP18.2) of Arabidopsis thaliana was inserted upstream o...

journal_title：Applied microbiology and biotechnology

authors： Yoshida K,Kasai T,Garcia MR,Sawada S,Shoji T,Shimizu S,Yamazaki K,Komeda Y,Shinmyo A

更新日期：1995-12-01 00:00:00

abstract：:Yarrowia lipolytica is an industrial yeast that has been used in the sustainable production of fatty acid-derived and lipid compounds due to its high growth capacity, genetic tractability, and oleaginous properties. This investigation examines the possibility of utilizing urea or urine as an alternative to ammonium su...

journal_title：Applied microbiology and biotechnology

authors： Brabender M,Hussain MS,Rodriguez G,Blenner MA

更新日期：2018-03-01 00:00:00

abstract：:We are beginning to see how the microbiota of the human gastrointestinal tract (GIT) can drive the development of new products to benefit human health and wellbeing. Despite the growing market for prebiotics and probiotics, there are currently no commercial products available that aid or increase the attachment of hea...

journal_title：Applied microbiology and biotechnology

authors： Morrin ST,Hickey RM

更新日期：2020-02-01 00:00:00

abstract：:Medicinal plants of the Dendrobium genus are highly prized, and hence, methodologies have been developed to authenticate Dendrobium drugs from its adulterants. Many bioactive constituents of Dendrobium species have been identified. The macromolecules included lectins; the enzymes chalcone synthase, sucrose synthase, a...

journal_title：Applied microbiology and biotechnology

authors： Ng TB,Liu J,Wong JH,Ye X,Wing Sze SC,Tong Y,Zhang KY

更新日期：2012-03-01 00:00:00

abstract：:The engineering of Corynebacterium glutamicum is important for enhanced production of biochemicals. To construct an optimal C. glutamicum genome, a precise site-directed gene integration method was developed by using a pair of mutant lox sites, one a right element (RE) mutant lox site and the other a left element (LE)...

journal_title：Applied microbiology and biotechnology

authors： Suzuki N,Inui M,Yukawa H

更新日期：2007-12-01 00:00:00

abstract：:Photothermal cancer therapy, as a prospective approach for local cancer treatment, is attracting increasing interests. In this paper, gold nanorods were conjugated with folate (folate/AuNRs), and their photothermal effects on hepatocellular carcinoma cell line (HepG2) using MTT assay, flow cytometry, as well as on the...

journal_title：Applied microbiology and biotechnology

authors： Jin H,Yang P,Cai J,Wang J,Liu M

更新日期：2012-06-01 00:00:00

abstract：:Due to the emerging environmental issues related to heavy metals, concern about the soil quality of farming lands near manufacturing district is increasing. Investigating the function of soil microorganisms exposed to long-term heavy metal contamination is meaningful and important for agricultural soil utilization. Th...

journal_title：Applied microbiology and biotechnology

authors： Deng L,Zeng G,Fan C,Lu L,Chen X,Chen M,Wu H,He X,He Y

更新日期：2015-10-01 00:00:00

abstract：:Saccharomyces cerevisiae is the organism of choice for many food and beverage fermentations because it thrives in high-sugar and high-ethanol conditions. However, the conditions encountered in bioethanol fermentation pose specific challenges, including extremely high sugar and ethanol concentrations, high temperature,...

journal_title：Applied microbiology and biotechnology

authors： Mukherjee V,Steensels J,Lievens B,Van de Voorde I,Verplaetse A,Aerts G,Willems KA,Thevelein JM,Verstrepen KJ,Ruyters S

更新日期：2014-11-01 00:00:00

abstract：:This paper studies the initial stages of iridescent tarnishes on titanium heat exchanger tubes in contact with running freshwater on the river Tagus in Spain for up to 20 months. Electrochemical impedance spectroscopy (EIS), scanning electron microscopy [(SEM with energy dispersive X-ray (EDX)] and X-ray photoelectron...

journal_title：Applied microbiology and biotechnology

authors： Moreno DA,Cano E,Ibars JR,Polo JL,Montero F,Bastidas JM

更新日期：2004-05-01 00:00:00

abstract：:Due to efficient photosynthetic capability, robust growth, and clear genetic background, cyanobacteria are recently used for production of different biofuel and biochemical molecules by genetic engineering and showed great potentials as the next-generation microbial cell factory. For improving the production of bio-pr...

journal_title：Applied microbiology and biotechnology

authors： Tan X,Liang F,Cai K,Lu X

更新日期：2013-07-01 00:00:00

abstract：:Developing the production of ectomycorrhizal (ECM) mushrooms in forest has become a challenge. Only a few ECM species are currently cultivable. Controlled mycorrhization practices offer promising advance to produce currently uncultivable ECM mushrooms. The persistence of the production of edible species, either cultiv...

journal_title：Applied microbiology and biotechnology

authors： Savoie JM,Largeteau ML

更新日期：2011-02-01 00:00:00

abstract：:Drought, high/low temperature, and salinity are abiotic stress factors accepted as the main reason for crop yield losses in a world with growing population and food price increases. Additional problems create nutrient limitations and particularly low P soil status. The problem of phosphate fertilizers, P plant nutriti...

journal_title：Applied microbiology and biotechnology

authors： Vassilev N,Eichler-Löbermann B,Vassileva M

更新日期：2012-08-01 00:00:00

abstract：:The production of biological indicators involving bacterial sporulation and multi-step downstream processes has been described. The goal of the present work was to use fermented material as the final product in a biological indicator, thereby reducing processing steps and costs. The performance of three different inex...

journal_title：Applied microbiology and biotechnology

authors： Sella SR,Guizelini BP,Zanello PH,Vandenberghe LP,Ribeiro CA,Minozzo JC,Soccol CR

更新日期：2012-01-01 00:00:00

abstract：:Thioredoxin is crucial for the maintenance of the redox status of cells of all types. Mammalian thioredoxin is secreted from various types of cells, although the mechanism underlying has not yet been clarified. Previously, we demonstrated that thioredoxin was released from Saccharomyces cerevisiae after treatment with...

journal_title：Applied microbiology and biotechnology

authors： Takeuchi Y,Nomura W,Ohdate T,Tamasu S,Masutani H,Murata K,Izawa S,Yodoi J,Inoue Y

更新日期：2007-07-01 00:00:00

abstract：:Forty-eight single-use stirred tank bioreactors on a 10-mL scale operated in a magnetically inductive driven bioreaction block and automated with a liquid handler were applied for discrimination of different riboflavin producing Bacillus subtilis strains based on their performances in the parallel fed-batch processes....

journal_title：Applied microbiology and biotechnology

authors： Vester A,Hans M,Hohmann HP,Weuster-Botz D

更新日期：2009-08-01 00:00:00

abstract：:The medicinal mushroom Agaricus blazei produced high amounts of laccase (up to 5,000 units l(-1)) in a complex, agitated liquid medium based on tomato juice, while only traces of the enzyme (<100 units l(-1)) were detected in synthetic glucose-based medium. Purification of the enzyme required three chromatographic ste...

journal_title：Applied microbiology and biotechnology

authors： Ullrich R,Huong le M,Dung NL,Hofrichter M

更新日期：2005-05-01 00:00:00

abstract：:Aromatic amino acid metabolism in yeast is an important source of secondary compounds that influence the aroma and flavour of alcoholic beverages and foods. Examples are the higher alcohol 2-phenylethanol, and its acetate ester, 2-phenylethyl acetate, which impart desirable floral aromas in wine, beer and baker's prod...

journal_title：Applied microbiology and biotechnology

authors： Cordente AG,Schmidt S,Beltran G,Torija MJ,Curtin CD

更新日期：2019-06-01 00:00:00

abstract：:The genes encoding the mevalonate-based farnesyl pyrophosphate (FPP) biosynthetic pathway were encoded in two operons and expressed in Escherichia coli to increase the production of sesquiterpenes. Inefficient translation of several pathway genes created bottlenecks and led to the accumulation of several pathway inter...

journal_title：Applied microbiology and biotechnology

authors： Nowroozi FF,Baidoo EE,Ermakov S,Redding-Johanson AM,Batth TS,Petzold CJ,Keasling JD

更新日期：2014-02-01 00:00:00

abstract：:At present, more than 100 strains of Lentinula edodes are cultivated on a commercial scale in China. A simple, reliable, and effective method to distinguish some commercial strains of the superior type from other commercial strains is very important for the Lentinula industry. In this study, 23 commercial strains of L...

journal_title：Applied microbiology and biotechnology

authors： Li HB,Wu XQ,Peng HZ,Fu LZ,Wei HL,Wu QQ,Jin QY,Li N

更新日期：2008-11-01 00:00:00

abstract：:Soil microbial autotrophs play a significant role in CO2 fixation in terrestrial ecosystem, particularly in vegetation-constrained ecosystems with environmental stresses, such as the Tibetan Plateau characterized by low temperature and high UV. However, soil microbial autotrophic communities and their driving factors ...

journal_title：Applied microbiology and biotechnology

authors： Guo G,Kong W,Liu J,Zhao J,Du H,Zhang X,Xia P

更新日期：2015-10-01 00:00:00

abstract：:Shikimic acid is an industrially important chiral compound used as a key ingredient in formulation of drug Oseltamivir phosphate (Tamiflu) for the treatment of swine/avian flu. The high cost and limited availability of shikimic acid isolated from plants has detained the use of this valuable building block of the drug....

journal_title：Applied microbiology and biotechnology

authors： Rawat G,Tripathi P,Saxena RK

更新日期：2013-05-01 00:00:00

abstract：:The photocatalytic properties of titanium dioxide are well known and have many applications including the removal of organic contaminants and production of self-cleaning glass. There is an increasing interest in the application of the photocatalytic properties of TiO(2) for disinfection of surfaces, air and water. Rev...

journal_title：Applied microbiology and biotechnology

authors： Foster HA,Ditta IB,Varghese S,Steele A

更新日期：2011-06-01 00:00:00

abstract：:To meet the demand for sustainable energy, aquifer thermal energy storage (ATES) is widely used in the subsurface in urban areas. However, contamination of groundwater, especially with chlorinated volatile organic compounds (CVOCs), is often being encountered. This is commonly seen as an impediment to ATES implementat...

journal_title：Applied microbiology and biotechnology

authors： Ni Z,van Gaans P,Smit M,Rijnaarts H,Grotenhuis T

更新日期：2016-04-01 00:00:00

abstract：:To improve the stability and catalytic efficiency of pyranose 2-oxidase (P2Ox) by molecular enzyme evolution, we cloned P2Ox cDNA by RACE-PCR from a cDNA library derived from the basidiomycete Peniophora gigantea. The P2Ox gene was expressed in Escherichia coli BL21(DE3), yielding an intracellular and enzymatically ac...

journal_title：Applied microbiology and biotechnology

authors： Bastian S,Rekowski MJ,Witte K,Heckmann-Pohl DM,Giffhorn F

更新日期：2005-06-01 00:00:00

abstract：:A series of continuous- and sequencing-batch reactor experiments were performed to assess the growth dynamics of Escherichia coli strain K12-MG1655 in chemostat systems. Previous mathematical predictions and early experimental results had shown that confined oscillatory dynamics ensue in bioreactor populations, which ...

journal_title：Applied microbiology and biotechnology

authors： Ofiţeru ID,Ferdeş M,Knapp CW,Graham DW,Lavric V

更新日期：2012-04-01 00:00:00