Abstract：
:The pyrene-degrading Mycobacterium strain AP1 was found to utilize fluoranthene as a sole source of carbon and energy. Identification of metabolites formed from fluoranthene (by growing cells and washed-cell suspensions), the kinetics of metabolite accumulation, and metabolite-feeding studies all indicated that strain AP1 oxidizes fluoranthene using three alternative routes. The first route is initiated by dioxygenation at C-7 and C-8 and, following meta cleavage and pyruvate release, produces a hydroxyacenaphthoic acid that is decarboxylated to acenaphthenone (V). Monooxygenation of this ketone to the quinone and subsequent hydrolysis generates naphthalene-1,8-dicarboxylic acid (IV), which is further degraded via benzene-1,2,3-tricarboxylic acid (III). A second route involves dioxygenation at C-1 and C-2, followed by dehydrogenation and meta cleavage of the resulting diol. A two-carbon fragment excision of the meta cleavage product yields 9-fluorenone-1-carboxylic acid (II), which appears to undergo angular dioxygenation and further degradation to produce benzene-1,2,3-tricarboxylic acid (III), merging this route with the 7,8-dioxygenation route. Decarboxylation of benzene-1,2,3-tricarboxylic acid to phthalate (VIII), as well as further oxidation of the latter, would connect both routes with the central metabolism. The identification of Z-9-carboxymethylenefluorene-1-carboxylic acid (I) suggests a third route for fluoranthene degradation involving dioxygenation at C-2, C-3, and ortho cleavage. There is no evidence of any further degradation of this compound.
journal_name
Appl Microbiol Biotechnoljournal_title
Applied microbiology and biotechnologyauthors
López Z,Vila J,Minguillón C,Grifoll Mdoi
10.1007/s00253-005-0120-9subject
Has Abstractpub_date
2006-05-01 00:00:00pages
747-56issue
6eissn
0175-7598issn
1432-0614journal_volume
70pub_type
杂志文章abstract：:A novel cell surface display system in Aspergillus oryzae was established by using a chitin-binding module (CBM) from Saccharomyces cerevisiae as an anchor protein. CBM was fused to the N or C terminus of green fluorescent protein (GFP) and the fusion proteins (GFP-CBM and CBM-GFP) were expressed using A. oryzae as a ...
journal_title：Applied microbiology and biotechnology
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pub_type： 杂志文章,评审
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journal_title：Applied microbiology and biotechnology
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更新日期：2007-08-01 00:00:00
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journal_title：Applied microbiology and biotechnology
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journal_title：Applied microbiology and biotechnology
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journal_title：Applied microbiology and biotechnology
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更新日期：2007-05-01 00:00:00
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doi：10.1007/s00253-018-9038-x
更新日期：2018-07-01 00:00:00
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journal_title：Applied microbiology and biotechnology
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doi：10.1007/BF00169420
更新日期：1992-10-01 00:00:00
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journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章,评审
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更新日期：2014-05-01 00:00:00
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journal_title：Applied microbiology and biotechnology
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更新日期：2013-11-01 00:00:00
