Studies on nutritional and oxygen requirements for production of L-asparaginase by Enterobacter aerogenes.

abstract：:Optimization of yields and productivities in reductive whole-cell biotransformations is an important issue for the industrial application of such processes. In a recent study with Escherichia coli, we analyzed the reduction of the prochiral β-ketoester methyl acetoacetate by an R-specific alcohol dehydrogenase (ADH) t...

journal_title：Applied microbiology and biotechnology

authors： Siedler S,Bringer S,Blank LM,Bott M

更新日期：2012-02-01 00:00:00

abstract：:Coastal sediments contaminated by polycyclic aromatic hydrocarbons (PAHs) can be candidates for remediation via an approach like land farming. Land farming converts naturally anaerobic sediments to aerobic environments, and the response of microbial communities, in terms of community structure alterations and correspo...

journal_title：Applied microbiology and biotechnology

authors： Obi CC,Adebusoye SA,Amund OO,Ugoji EO,Ilori MO,Hedman CJ,Hickey WJ

更新日期：2017-05-01 00:00:00

abstract：:Vitamin B12 is an essential nutrient required for crucial metabolic processes in humans. Vitamin B12-producing lactic acid bacteria (LAB) have been attracting increased attentions currently because of the generally recognized as safe (GRAS) status. Most of recent studies focused on Lactobacillus, and little is known a...

journal_title：Applied microbiology and biotechnology

authors： Li P,Gu Q,Wang Y,Yu Y,Yang L,Chen JV

更新日期：2017-08-01 00:00:00

abstract：:Enzymes are natural catalysts highly specific to the substrate type and operate under mild conditions of temperature, pressure, and pH with high conversion rates, which makes them more efficient than conventional chemical catalysts. The enzymes can be obtained from various sources, animal, vegetable, and microbiologic...

journal_title：Applied microbiology and biotechnology

authors： Filho DG,Silva AG,Guidini CZ

更新日期：2019-09-01 00:00:00

abstract：:Smokeless tobacco (ST) products are used worldwide and are a major public health concern. In addition to harmful chemicals found in these products, microbes found in ST products are believed to be responsible for generating harmful tobacco-specific nitrosamines (TSNAs), the most abundant carcinogens in ST. These micro...

journal_title：Applied microbiology and biotechnology

authors： Tyx RE,Rivera AJ,Keong LM,Stanfill SB

更新日期：2020-01-01 00:00:00

abstract：:Organophosphate hydrolase containing hexahistidine tag at the N-terminus of recombinant protein (His6-OPH) and expressed in Escherichia coli cells was purified using supermacroporous polyacrylamide-based monolith columns with immobilized metal affinity matrices [Me2+-iminodiacetic acid (IDA)-polyacrylamide cryogel (PA...

journal_title：Applied microbiology and biotechnology

authors： Efremenko E,Votchitseva Y,Plieva F,Galaev I,Mattiasson B

更新日期：2006-05-01 00:00:00

abstract：:Epoxide hydrolases (EHs) of fungal origin have the ability to catalyze the enantioselective hydrolysis of epoxides to their corresponding diols. However, wild type fungal EHs are limited in substrate range and enantioselectivity. Additionally, the production of fungal epoxide hydrolase (EH) by wild-type strains is typ...

journal_title：Applied microbiology and biotechnology

authors： Maharajh D,Roth R,Lalloo R,Simpson C,Mitra R,Görgens J,Ramchuran S

更新日期：2008-05-01 00:00:00

abstract：:D-Xylonic acid is a versatile platform chemical with reported applications as complexing agent or chelator, in dispersal of concrete, and as a precursor for compounds such as co-polyamides, polyesters, hydrogels and 1,2,4-butanetriol. With increasing glucose prices, D-xylonic acid may provide a cheap, non-food derived...

journal_title：Applied microbiology and biotechnology

authors： Toivari MH,Nygård Y,Penttilä M,Ruohonen L,Wiebe MG

更新日期：2012-10-01 00:00:00

abstract：:Carboligations catalyzed by aldolases or thiamine diphosphate (ThDP)-dependent enzymes are well-known in biocatalysis to deliver enantioselective chain elongation reactions. A pyruvate-dependent aldolase (2-oxo-3-deoxy-6-phosphogluconate aldolase [EDA]) introduces a chiral center when reacting with the electrophile, g...

journal_title：Applied microbiology and biotechnology

authors： Schapfl M,Baier S,Fries A,Ferlaino S,Waltzer S,Müller M,Sprenger GA

更新日期：2018-10-01 00:00:00

abstract：:Hepatic lipid metabolic disorders and oxidative stress are involved in the development of non-alcoholic fatty liver disease (NAFLD). This study is to determine the protective effects of Lactobacillus plantarum NA136 on high-fat diet and fructose (HFD/F)-induced NAFLD and to elucidate its underlying molecular mechanism...

journal_title：Applied microbiology and biotechnology

authors： Zhao Z,Wang C,Zhang L,Zhao Y,Duan C,Zhang X,Gao L,Li S

更新日期：2019-07-01 00:00:00

abstract：:A general procedure for the high yield immobilization of enzymes with the help of specific anti-enzyme antibodies is described. Polyclonal antibodies were raised against Aspergillus niger glucose oxidase and horseradish peroxidase in rabbits and the gamma globulin (IgG) fraction from the immune sera isolated by ammoni...

journal_title：Applied microbiology and biotechnology

authors： Farooqi M,Sosnitza P,Saleemuddin M,Ulber R,Scheper T

更新日期：1999-09-01 00:00:00

abstract：:The alphaproteobacterial Roseobacter clade (Rhodobacterales) is one of the most important global players in carbon and sulfur cycles of marine ecosystems. The remarkable metabolic versatility of this bacterial lineage provides access to diverse habitats and correlates with a multitude of extrachromosomal elements. Fou...

journal_title：Applied microbiology and biotechnology

authors： Petersen J,Frank O,Göker M,Pradella S

更新日期：2013-04-01 00:00:00

abstract：:There is a worldwide trend to understand the impact of non-Saccharomyces yeast species on the process of winemaking. Although the predominant species at the end of the fermentation is Saccharomyces cerevisiae, several non-Saccharomyces species present during the first days of the process can produce and/or release aro...

journal_title：Applied microbiology and biotechnology

authors： Barquet M,Martín V,Medina K,Pérez G,Carrau F,Gaggero C

更新日期：2012-01-01 00:00:00

abstract：:Phytases are phosphohydrolases that initiate the sequential hydrolysis of phosphate from phytate, which is the main storage form of phosphorous in numerous plant seeds, especially in cereals and grains. Phytate is indigestible for most monogastric animals, such as poultry, swine, fish, and humans; therefore, microbial...

journal_title：Applied microbiology and biotechnology

authors： Körfer G,Novoa C,Kern J,Balla E,Grütering C,Davari MD,Martinez R,Vojcic L,Schwaneberg U

更新日期：2018-11-01 00:00:00

abstract：:For ethical, technical, regulatory, and cost reasons, in vitro methods are increasingly used as an alternative to in vivo experimentations. The aim of the present study was to validate, according to in vivo data in living animals, a new in vitro model of the piglet colon, the PigutIVM, under both control conditions an...

journal_title：Applied microbiology and biotechnology

authors： Fleury MA,Le Goff O,Denis S,Chaucheyras-Durand F,Jouy E,Kempf I,Alric M,Blanquet-Diot S

更新日期：2017-03-01 00:00:00

abstract：:Winemaking, brewing and baking are some of the oldest biotechnological processes. In all of them, alcoholic fermentation is the main biotransformation and Saccharomyces cerevisiae the primary microorganism. Although a wide variety of microbial species may participate in alcoholic fermentation and contribute to the sen...

journal_title：Applied microbiology and biotechnology

authors： Albergaria H,Arneborg N

更新日期：2016-03-01 00:00:00

abstract：:The Gram-positive Corynebacterium glutamicum is widely used for fermentative production of amino acids. The world production of L-lysine has surpassed 2 million tons per year. Glucose uptake and phosphorylation by C. glutamicum mainly occur by the phosphotransferase system (PTS) and to lesser extent by inositol permea...

journal_title：Applied microbiology and biotechnology

authors： Pérez-García F,Peters-Wendisch P,Wendisch VF

更新日期：2016-09-01 00:00:00

abstract：:The host innate immune response either clears invading viruses or allows the adaptive immune system to establish an effective antiviral response. In this study, both pathogenic (passage 3, P3) and attenuated (P110) infectious bronchitis virus (IBV) strains were used to study the immune responses of chicken to IBV infe...

journal_title：Applied microbiology and biotechnology

authors： Xu Y,Zhang T,Xu Q,Han Z,Liang S,Shao Y,Ma D,Liu S

更新日期：2015-11-01 00:00:00

abstract：:Because engineering of the 101.016-bp megaplasmid pKB1 of Gordonia westfalica Kb1 failed due to the absence of an effective transfer system, pKB1 was transferred by conjugation from G. westfalica Kb1 to a kanamycin-resistant mutant of Rhodococcus opacus PD630 at a frequency of about 6.2 x 10(-8) events per recipient c...

journal_title：Applied microbiology and biotechnology

authors： Bröker D,Arenskötter M,Steinbüchel A

更新日期：2008-01-01 00:00:00

abstract：:Recently, it was shown that electrical DNA-chips in connection with a magnetic bead-based sandwich-hybridization assay can be a suitable alternative for the analysis of gene expression by monitoring the respective mRNA levels. In this study, we established an improved assay which allowed for a significantly shortened ...

journal_title：Applied microbiology and biotechnology

authors： Pioch D,Jürgen B,Evers S,Maurer KH,Hecker M,Schweder T

更新日期：2008-03-01 00:00:00

abstract：:Superoxide dismutase (SOD) activity is one major defense line against oxidative stress for all of the aerobic organisms, and industrial production of this enzyme is highly demanded. The Cu/Zn superoxide dismutase gene (KmSOD1) of Kluyveromyces marxianus L3 was cloned and characterized. The deduced KmSod1p protein shar...

journal_title：Applied microbiology and biotechnology

authors： Raimondi S,Uccelletti D,Matteuzzi D,Pagnoni UM,Rossi M,Palleschi C

更新日期：2008-01-01 00:00:00

abstract：:Two recombinant Aeropyrum pernix glutamate dehydrogenase (GDH) enzymes with different length N-termini were cloned and expressed in Escherichia coli: sGDH begins with the amino acid sequence of the extracted native enzyme (M-Q-P-T-D-P-L-E-E), whereas lGDH begins with the sequence of the predicted ORF (M-E-V-L-A-L-Q-P-...

journal_title：Applied microbiology and biotechnology

authors： Helianti I,Morita Y,Murakami Y,Yokoyama K,Tamiya E

更新日期：2002-08-01 00:00:00

abstract：:Thraustochytrium sp. 26185, a unicellular marine protist, synthesizes docosahexaenoic acid, an omega-3 very long chain polyunsaturated fatty acid (VLC-PUFAs), by a polyunsaturated fatty acid (PUFA) synthase comprising three large subunits with multiple catalytic dehydratase (DH) domains critical for introducing double...

journal_title：Applied microbiology and biotechnology

authors： Xie X,Meesapyodsuk D,Qiu X

更新日期：2018-01-01 00:00:00

abstract：:Under alternating aerobic/anaerobic conditions and without additional carbon sources, a bacterial consortium consisting initially of 18 bacterial strains was obtained in a sequence batch reactor. The phosphorus removal capability could only be maintained using sterile filtrate of activated sludge as medium. The additi...

journal_title：Applied microbiology and biotechnology

authors： Hollender J,Dreyer U,Kornberger L,Kämpfer P,Dott W

更新日期：2002-01-01 00:00:00

abstract：:Pneumocandin B0, the precursor of the antifungal drug caspofungin, is a secondary metabolite of the fungus Glarea lozoyensis. In this study, we investigated the effects of mannitol as the sole carbon source on pneumocandin B0 production by G. lozoyensis. The osmotic pressure is more important in enhancing pneumocandin...

journal_title：Applied microbiology and biotechnology

authors： Song P,Huang B,Zhang S,Zhang K,Yuan K,Ji X,Ren L,Wen J,Huang H

更新日期：2018-12-01 00:00:00

abstract：:Two bacterial strains, Ralstonia sp. LD35 and Pseudomonas putida DSM 1868, were assayed for their ability to degrade the monocyclic aromatic compounds commonly found in olive mill wastewaters (OMWs). The goal was to study the possibility of employing the two strains in the removal of these recalcitrant and toxic compo...

journal_title：Applied microbiology and biotechnology

authors： Di Gioia D,Fava F,Bertin L,Marchetti L

更新日期：2001-05-01 00:00:00

abstract：:4AaCter is the polypeptide from the C-terminal extension of mosquitocidal Cry4Aa toxin, and facilitates formation of protein inclusion in Escherichia coli. It has been demonstrated that the use of 4AaCter as a peptide tag results in the efficient production of heterologous protein in E. coli. It has also been demonstr...

journal_title：Applied microbiology and biotechnology

authors： Hayakawa T,Shimizu Y,Ishida T,Sakai H

更新日期：2011-06-01 00:00:00

abstract：:Acrylic polymers (AP) are a diverse group of materials with broad applications, frequent use, and increasing demand. Some of the most used AP are polyacrylamide, polyacrylic acid, polymethyl methacrylates, and polyacrylonitrile. Although no information for the production of all AP types is published, data for the most...

journal_title：Applied microbiology and biotechnology

authors： Gaytán I,Burelo M,Loza-Tavera H

更新日期：2021-01-11 00:00:00

abstract：:The purpose of this paper is to present biomass and nutrient uptake data from Neochloris oleoabundans production in an open trough system. The growth medium used was BG11, temperature ranged from 16.7 °C to 25.3 °C, and pH ranged from 5.52 to 9.94 because the customary pH increase during algal biomass production was m...

journal_title：Applied microbiology and biotechnology

authors： Murray KE,Healy FG,McCord RS,Shields JA

更新日期：2011-04-01 00:00:00

abstract：:A Gram-negative anaerobic microorganism, MRG-1, isolated from human intestine showed high activities of deglycosylation and reduction of daidzin, based on rapid TLC analysis. A rod-shaped strain MRG-1 was identified as a new species showing 91.0% homology to Coprobacillus species, based on 16S rRNA sequence analysis. ...

journal_title：Applied microbiology and biotechnology

authors： Park HY,Kim M,Han J

更新日期：2011-08-01 00:00:00