Purification of His6-organophosphate hydrolase using monolithic supermacroporous polyacrylamide cryogels developed for immobilized metal affinity chromatography.

abstract：:The filamentous fungus Aspergillus flavus is an agriculturally important opportunistic plant pathogen that produces potent carcinogenic compounds called aflatoxins. We identified the A. flavus rtfA gene, the ortholog of rtf1 in Saccharomyces cerevisiae and rtfA in Aspergillus nidulans. Interestingly, rtfA has multiple...

journal_title：Applied microbiology and biotechnology

authors： Lohmar JM,Harris-Coward PY,Cary JW,Dhingra S,Calvo AM

更新日期：2016-06-01 00:00:00

abstract：:The effect of vitamins on the aerobic degradation and dechlorination of 2-chlorophenol and 4-chlorophenol by Pseudomonas pickettii, strain LD1, and 4-chlorobiphenyl by Pseudomonas sp. strain CPE1 was determined. These microorganisms are capable of using the target compounds as the sole carbon and energy source, but do...

journal_title：Applied microbiology and biotechnology

authors： Kafkewitz D,Fava F,Armenante PM

更新日期：1996-11-01 00:00:00

abstract：:Entomopathogenic fungi are widely produced for use as mycoinsecticides. Therefore, improvement of the shelf life of fungal propagules under good and adverse conditions should be a pre-requisite of their production. In order to improve conidial physiology as well as mycoinsecticide efficiency, culture conditions may be...

journal_title：Applied microbiology and biotechnology

authors： Tarocco F,Lecuona RE,Couto AS,Arcas JA

更新日期：2005-09-01 00:00:00

abstract：:To clarify the effect of type of foods on the intestinal environment, Far East Asian- (FEA; rich in rice starch, soy protein and soy oil) and Far East Asian marine- (FEAM; rich in rice starch, fish meal, fish oil and brown alga) modelled diets and sucrose, casein and beef tallow-rich (SCB) diet were prepared. After th...

journal_title：Applied microbiology and biotechnology

authors： An C,Kuda T,Yazaki T,Takahashi H,Kimura B

更新日期：2014-05-01 00:00:00

abstract：:Vibrio parahaemolyticus is one of the major pathogens responsible for vibriosis and zoonotic infections in teleosts, marine invertebrates, and also humans through consumption of contaminated or unprocessed seafood. Emergence of resistance against current accessible antibiotics and spread to the food chain and environm...

journal_title：Applied microbiology and biotechnology

authors： Karan S,Choudhury D,Dixit A

更新日期：2021-01-01 00:00:00

abstract：:The polyene macrolide rimocidin, produced by Streptomyces rimosus M527, was found to be highly effective against a broad range of fungal plant pathogens. Current understanding of the regulatory mechanism of rimocidin biosynthesis and morphological differentiation in S. rimosus M527 is limited. NsdA is considered a neg...

journal_title：Applied microbiology and biotechnology

authors： Liao Z,Song Z,Xu J,Ma Z,Bechthold A,Yu X

更新日期：2020-12-01 00:00:00

abstract：:The lack of a universal method to extract RNA from soil hinders the progress of studies related to nitrification in soil, which is an important step in the nitrogen cycle. It is particularly difficult to extract RNA from certain types of soils such as Andosols (volcanic ash soils), which is the dominant agricultural s...

journal_title：Applied microbiology and biotechnology

authors： Wang Y,Nagaoka K,Hayatsu M,Sakai Y,Tago K,Asakawa S,Fujii T

更新日期：2012-11-01 00:00:00

abstract：:Streptomyces is an interesting host for the secretory production of recombinant proteins because of its innate capacity to secrete proteins at high level in the culture medium. In this report, we evaluated the importance of the phage-shock protein A (PspA) homologue on the protein secretion yield in Streptomyces livid...

journal_title：Applied microbiology and biotechnology

authors： Vrancken K,De Keersmaeker S,Geukens N,Lammertyn E,Anné J,Van Mellaert L

更新日期：2007-01-01 00:00:00

abstract：:The gene encoding NADP(+)-dependent L-1-amino-2-propanol dehydrogenase (AADH) of Rhodococcus erythropolis MAK154 was cloned and sequenced. A 780-bp nucleotide fragment was confirmed to be the gene encoding AADH by agreement of the N-terminal and internal amino acid sequences of the purified AADH. The gene (aadh) codes...

journal_title：Applied microbiology and biotechnology

authors： Kataoka M,Ishige T,Urano N,Nakamura Y,Sakuradani E,Fukui S,Kita S,Sakamoto K,Shimizu S

更新日期：2008-09-01 00:00:00

abstract：:Recently, a new gene encoding beta-glucuronidase from Streptococcus equi subsp. zooepidemicus (SEZ) was identified and expressed in Escherichia coli. In this paper, the characterization of the enzyme is described. Specific enzyme activity was 120,000 U/mg purified protein at 37 degrees C and pH = 7.0. The temperature ...

journal_title：Applied microbiology and biotechnology

authors： Krahulec J,Krahulcová J

更新日期：2007-04-01 00:00:00

abstract：:The 16S ribosomal RNA (rRNA) gene has been the most commonly used sequence to characterize bacterial communities. The classical approach to obtain gene sequences to study bacterial diversity implies cloning amplicons, selecting clones, and Sanger sequencing cloned fragments. A more recent approach is direct sequencing...

journal_title：Applied microbiology and biotechnology

authors： Assunção A,Costa MC,Carlier JD

更新日期：2016-03-01 00:00:00

abstract：:In order to enhance the removal efficiency of chemical oxygen demand (COD) in the high-salinity landfill leachate, the dominant strains were isolated from high-salinity landfill leachate. The dominant strains and bacteria consortium were screened for COD treatment potential using an aerobic COD concentration decrease ...

journal_title：Applied microbiology and biotechnology

authors： Guo L,Ji M,Dong H,Wei Y

更新日期：2009-08-01 00:00:00

abstract：:Batch assays are currently used to study the kinetic behavior of microbial growth. However, it has been shown that the outcome of batch experiments is greatly influenced by the initial ratio of substrate concentration (S(o)) to biomass concentration (Xo). Substrate-sufficient batch culture is known to have mechanisms ...

journal_title：Applied microbiology and biotechnology

authors： Liu Y,Chen GH,Rols JL

更新日期：1999-11-01 00:00:00

abstract：:The rapid global urbanization and other extensive anthropogenic activities exacerbated the worldwide human health risks induced by antibiotic resistance genes (ARGs). Knowledge of the origins and dissemination of ARGs is essential for understanding modern resistome, while little information is known regarding the over...

journal_title：Applied microbiology and biotechnology

authors： Ouyang WY,Huang FY,Zhao Y,Li H,Su JQ

更新日期：2015-07-01 00:00:00

abstract：:This study found that the ratio of Thiothrix eikelboomii to total bacterial concentrations (TH/TB) (%) was a better indicator of bulking incidents affecting effluent quality compared to absolute T. eikelboomii abundance alone. This was determined using a genus-specific Thiothrix quantitative PCR primer and probe set, ...

journal_title：Applied microbiology and biotechnology

authors： Asvapathanagul P,Olson BH,Gedalanga PB,Hashemi A,Huang Z,La J

更新日期：2015-05-01 00:00:00

abstract：:The gene encoding a putative triphenylmethane reductase (TMR)-like protein derived from Geobacillus thermoglucosidasius C56-Y593 (named as GtAZR) was synthesized, heterologously expressed in Escherichia coli, and extensively characterized for the first time. The recombinant GtAZR displayed its maximum activity at pH 5...

journal_title：Applied microbiology and biotechnology

authors： Gao F,Ding H,Shao L,Xu X,Zhao Y

更新日期：2015-01-01 00:00:00

abstract：:The extracellular production of Escherichia coli phytase was studied in fed-batch fermentations. Two different feeding strategies were compared: control by keeping the glucose concentration constant, and control by keeping a low constant oxygen level in the medium. For the feeding control based on glucose concentratio...

journal_title：Applied microbiology and biotechnology

authors： Kleist S,Miksch G,Hitzmann B,Arndt M,Friehs K,Flaschel E

更新日期：2003-06-01 00:00:00

abstract：:Fluorescence in situ hybridization (FISH) employing nucleic acid mimics as probes is becoming an emerging molecular tool in the microbiology area for the detection and visualization of microorganisms. However, the impact that locked nucleic acid (LNA) and 2'-O-methyl (2'-OMe) RNA modifications have on the probe that i...

journal_title：Applied microbiology and biotechnology

authors： Fontenete S,Carvalho D,Guimarães N,Madureira P,Figueiredo C,Wengel J,Azevedo NF

更新日期：2016-07-01 00:00:00

abstract：:A system for the controlled expression of a foreign gene in cultured tobacco cells (Nicotiana tabacum, BY2) by temperature shift was constructed. A 925-base-pair (bp) DNA fragment containing the 5'-flanking region of a low-molecular-mass heat-shock protein gene (HSP18.2) of Arabidopsis thaliana was inserted upstream o...

journal_title：Applied microbiology and biotechnology

authors： Yoshida K,Kasai T,Garcia MR,Sawada S,Shoji T,Shimizu S,Yamazaki K,Komeda Y,Shinmyo A

更新日期：1995-12-01 00:00:00

abstract：:Biodegradation can achieve complete and cost-effective elimination of aromatic pollutants through harnessing diverse microbial metabolic processes. Aromatics biodegradation plays an important role in environmental cleanup and has been extensively studied since the inception of biodegradation. These studies, however, a...

journal_title：Applied microbiology and biotechnology

authors： Cao B,Nagarajan K,Loh KC

更新日期：2009-11-01 00:00:00

abstract：:This study evaluated the effect of inorganic mercury (Hg) on bacterial community and diversity in different soils. Three soils-neutral, alkaline and acidic-were spiked with six different concentrations of Hg ranging from 0 to 200 mg kg-1 and aged for 90 days. At the end of the ageing period, 18 samples from three diff...

journal_title：Applied microbiology and biotechnology

authors： Mahbub KR,Subashchandrabose SR,Krishnan K,Naidu R,Megharaj M

更新日期：2017-03-01 00:00:00

abstract：:Orange peel waste (OPW), the primary byproduct of the juice extraction process, is annually generated in massive amounts (21 Mton), and its aqueous extraction in biorefining operations yields a liquid fraction, referred to as orange peel extract (OPE). Although OPE contains significant amounts of easily assimilable ca...

journal_title：Applied microbiology and biotechnology

authors： Carota E,Petruccioli M,D'Annibale A,Gallo AM,Crognale S

更新日期：2020-05-01 00:00:00

abstract：:Ideal immunogenicity in antigens is a prerequisite to eliciting a sufficiently strong immune and memory response via either DNA or protein vaccines. To improve immunogenicity, efforts have focused on high-level expression of target proteins and on maintaining their natural conformations. In the present work, two trime...

journal_title：Applied microbiology and biotechnology

authors： Wang JY,Song WT,Li Y,Chen WJ,Yang D,Zhong GC,Zhou HZ,Ren CY,Yu HT,Ling H

更新日期：2011-08-01 00:00:00

abstract：:Panax ginseng C.A. Meyer (ginseng) is a well-known medicinal plant that has been traditionally used in the oriental countries for centuries. Wild ginseng is a scarce and rare commodity. Field cultivation of the ginseng plant is a time-consuming and labor-intensive process. Ginsenosides, a group of glycosylated triterp...

journal_title：Applied microbiology and biotechnology

authors： Murthy HN,Georgiev MI,Kim YS,Jeong CS,Kim SJ,Park SY,Paek KY

更新日期：2014-01-01 00:00:00

abstract：:Respiration measurement in shake flasks is introduced as a new method to characterize the metabolic activity of microorganisms during and after stress exposure. The major advantage of the new method is the possibility to determine the metabolic activity independent of manual sampling without the necessity to change th...

journal_title：Applied microbiology and biotechnology

authors： Seletzky JM,Noack U,Fricke J,Hahn S,Büchs J

更新日期：2006-10-01 00:00:00

abstract：:Ferulic acid was efficiently released from a wheat bran preparation by a ferulic acid esterase from Aspergillus niger (FAE-III) when incubated together with a Trichoderma viride xylanase (a maximum of 95% total ferulic acid released after 5 h incubation). FAE-III by itself could release ferulic acid but at a level alm...

journal_title：Applied microbiology and biotechnology

authors： Faulds CB,Williamson G

更新日期：1995-11-01 00:00:00

abstract：:Starting from mature vegetable compost, four bacterial strains were selected using a lignin-rich medium. 16S ribosomal RNA identification of the isolates showed high score similarity with Pseudomonas spp. for three out of four isolates. Further characterization of growth on mixtures of six selected lignin model compou...

journal_title：Applied microbiology and biotechnology

authors： Ravi K,García-Hidalgo J,Gorwa-Grauslund MF,Lidén G

更新日期：2017-06-01 00:00:00

abstract：:The alkali-soluble glucan of the yeast cell wall contains beta-(1,3)- and (1,6)-D-linkages and systemically enhances the immune system. To isolate Saccharomyces cerevisiae mutants producing glucan with a high degree of beta-(1,6)-D-glycosidic bonds, a wild-type strain was mutagenized with ultraviolet light. The mutant...

journal_title：Applied microbiology and biotechnology

authors： Ha CH,Lim KH,Kim YT,Lim ST,Kim CW,Chang HI

更新日期：2002-03-01 00:00:00

abstract：:An enzyme preparation from the thermophilic fungus Humicola insolens, Ultraflo L, was able to solubilise more than half of the biomass of brewer's grain and wheat bran, two agro-industrial co-products. While almost all of the ferulic acid was released in the free form, the majority of diferulates were released still a...

journal_title：Applied microbiology and biotechnology

authors： Faulds CB,Mandalari G,LoCurto R,Bisignano G,Waldron KW

更新日期：2004-06-01 00:00:00

abstract：:Three different mechanisms can be envisaged that are used by fungi to solubilize coal: the production of alkaline substances, the extrusion of chelators and, of special interest in the scope of biotechnology, the action of enzymes. Whether these mechanisms are operating separately or in various combinations has not ye...

journal_title：Applied microbiology and biotechnology

authors： Holker U,Ludwig S,Scheel T,Hofer M

更新日期：1999-07-01 00:00:00