Application of locked nucleic acid-based probes in fluorescence in situ hybridization.

abstract：:Mating tests, internal transcribed spacer (ITS) sequence analysis, intergenic spacer 1-restriction fragment length polymorphism (IGS1-RFLP), IGS1 sequence analysis, and IGS2-RFLP analysis were carried out on isolates of 17 morphologically different Pleurotus mushrooms collected on Ferula sinkiangensis. The isolates we...

journal_title：Applied microbiology and biotechnology

authors： Zhang JX,Huang CY,Ng TB,Wang HX

更新日期：2006-07-01 00:00:00

abstract：:Some strains belonging to the genera Citrobacter and Enterobacter have been reported to produce chitin/chitosan-like bioflocculants (BFs) from acetate. In this study, to investigate the distribution of the BF-producing potential in the genus Citrobacter and to screen stably and highly BF-producing strains, we obtained...

journal_title：Applied microbiology and biotechnology

authors： Kimura K,Inoue T,Kato D,Negoro S,Ike M,Takeo M

更新日期：2013-11-01 00:00:00

abstract：:A metabolic heat-based model was used for estimating the growth of Kluyveromyces marxianus, and the modified Luedeking-Piret kinetic model was used for describing the inulinase production kinetics. For the first time, a relationship was developed to relate inulinase production kinetics directly to metabolic heat gener...

journal_title：Applied microbiology and biotechnology

authors： Santharam L,Samuthirapandi AB,Easwaran SN,Mahadevan S

更新日期：2017-03-01 00:00:00

abstract：:Exophiala jeanselmei is an opportunistic pathogenic black yeast growing in humid environments such as water reservoirs of air-conditioning systems. Because this fungal contaminant could be vaporized into the air and subsequently cause health problems, its monitoring is recommended. Currently, this monitoring is based ...

journal_title：Applied microbiology and biotechnology

authors： Libert X,Chasseur C,Packeu A,Bureau F,Roosens NH,De Keersmaecker SJC

更新日期：2016-02-01 00:00:00

abstract：:Phytophthora infestans is the causative agent of potato blight that resulted in the great famine in Ireland in the nineteenth century. This microbe can release large amounts of the C20 very long-chain polyunsaturated fatty acids arachidonic acid (ARA; 20:4Δ(5, 8, 11, 14)) and eicosapentaenoic acid (EPA; 20:5Δ(5, 8, 11...

journal_title：Applied microbiology and biotechnology

authors： Sun Q,Liu J,Zhang Q,Qing X,Dobson G,Li X,Qi B

更新日期：2013-09-01 00:00:00

abstract：:To seek more information on function of two-component regulatory systems (TCSs) in Streptomyces coelicolor, a dozen TCS-knockout mutants were generated, and phenotype changes were determined. One TCS (SCO5403/5404)-deleted mutant with phenotype change was obtained. Here, we report the characterization of this novel TC...

journal_title：Applied microbiology and biotechnology

authors： Lu Y,Wang W,Shu D,Zhang W,Chen L,Qin Z,Yang S,Jiang W

更新日期：2007-12-01 00:00:00

abstract：:Xylose is, after glucose, the dominant sugar in agricultural wastes. In anaerobic environments, carbohydrates are converted into volatile fatty acids and alcohols. These can be used as building blocks in biotechnological or chemical processes, e.g., to produce bioplastics. In this study, xylose fermentation by mixed m...

journal_title：Applied microbiology and biotechnology

authors： Temudo MF,Mato T,Kleerebezem R,van Loosdrecht MC

更新日期：2009-02-01 00:00:00

abstract：:A gene encoding beta-glucanase activity from Bacillus amyloliquefaciens was subcloned in both orientations into plasmid shuttle vector pSA3. In only one orientation could a co-integrate be generated with the conjugative plasmid pVA797. The plasmid co-integrate was conjugated into Lactobacillus helveticus strain CNRZ45...

journal_title：Applied microbiology and biotechnology

authors： Thompson K,Collins M

更新日期：1991-06-01 00:00:00

abstract：:The osmotic tolerance of cells of Saccharomyces cerevisiae as a function of glycerol concentration and temperature has been investigated. Results show that under isothermal conditions (25 degrees C) cells are resistant (94% viability) to hyperosmotic treatment at 49.2 MPa. A thigher osmotic pressure, cell viability de...

journal_title：Applied microbiology and biotechnology

authors： Beney L,Marechal PA,Gervais P

更新日期：2001-08-01 00:00:00

abstract：:Elimination of acetate overflow in aerobic cultivation of Escherichia coli would improve many bioprocesses as acetate accumulation in the growth environment leads to numerous negative effects, e.g. loss of carbon, inhibition of growth, target product synthesis, etc. Despite many years of studies, the mechanism and reg...

journal_title：Applied microbiology and biotechnology

authors： Peebo K,Valgepea K,Nahku R,Riis G,Oun M,Adamberg K,Vilu R

更新日期：2014-06-01 00:00:00

abstract：:Proteolytic enzymes play essential metabolic and regulatory functions in many biological processes and also offer a wide range of biotechnological applications. Because of their essential roles, their proteolytic activity needs to be tightly regulated. Therefore, small molecules and proteins that inhibit proteases can...

journal_title：Applied microbiology and biotechnology

authors： Sabotič J,Kos J

更新日期：2012-02-01 00:00:00

abstract：:Transcription factors have been studied as an important drug candidate. Ever since the successful generation of induced pluripotent stem cells (iPSCs), there has been tremendous interest in reprogramming transcription factors. Because of the safety risks involved in a virus-based approach, many researchers have been t...

journal_title：Applied microbiology and biotechnology

authors： Ryu J,Park HH,Park JH,Lee HJ,Rhee WJ,Park TH

更新日期：2016-04-01 00:00:00

abstract：:Recently, it was shown that electrical DNA-chips in connection with a magnetic bead-based sandwich-hybridization assay can be a suitable alternative for the analysis of gene expression by monitoring the respective mRNA levels. In this study, we established an improved assay which allowed for a significantly shortened ...

journal_title：Applied microbiology and biotechnology

authors： Pioch D,Jürgen B,Evers S,Maurer KH,Hecker M,Schweder T

更新日期：2008-03-01 00:00:00

abstract：:Streptococcus mutans is the main cause of dental caries. We evaluated the therapeutic effect of variable regions of a llama heavy chain antibody fragments directed against S. mutans named S36-VHH (S for Streptococcus) alone or fused with glucose oxidase (GOx) from Aspergillus niger. Western blot analysis and ELISA rev...

journal_title：Applied microbiology and biotechnology

authors： Krüger C,Hultberg A,Marcotte H,Hermans P,Bezemer S,Frenken LG,Hammarström L

更新日期：2006-10-01 00:00:00

abstract：:The broad-spectrum organophosphate hydrolase (OPH; EC 3.1.8.1) encoded by the organophosphate-degrading gene (opd) from Pseudomonas diminuta MG and Flavobacterium sp. ATCC 27551 possesses capabilities of both P-O bond hydrolysis (e.g. paraoxon) and P-F bond hydrolysis [e.g. sarin and diisopropylfluorophosphate (DFP)]....

journal_title：Applied microbiology and biotechnology

authors： Dave KI,Lauriano C,Xu B,Wild JR,Kenerley CM

更新日期：1994-05-01 00:00:00

abstract：:Yeast are important production platforms for the generation of recombinant proteins. Nonetheless, their use has been restricted in the production of therapeutic proteins due to differences in their glycosylation profile with that of higher eukaryotes. The yeast strain Pichia pastoris is an industrially important organ...

journal_title：Applied microbiology and biotechnology

authors： Hopkins D,Gomathinayagam S,Lynaugh H,Stadheim TA,Hamilton SR

更新日期：2014-03-01 00:00:00

abstract：:Fat, oil and, grease (FOG) deposits are one primary cause of sanitary sewer overflows (SSOs). While numerous studies have examined the formation of FOG deposits in sewer pipes, little is known about their biodegradation under sewer environments. In this study, FOG deposit biodegradation potential was determined by stu...

journal_title：Applied microbiology and biotechnology

authors： He X,Zhang Q,Cooney MJ,Yan T

更新日期：2015-07-01 00:00:00

abstract：:Functional screening for lipolytic enzymes at low temperatures resulted in the isolation of the novel cold-active esterases, EstM-N1 and EstM-N2, from a metagenomic DNA library of arctic soil samples. EstM-N1 and EstM-N2 were 395 and 407 amino acids in length, respectively, and showed the highest similarity to class C...

journal_title：Applied microbiology and biotechnology

authors： Yu EY,Kwon MA,Lee M,Oh JY,Choi JE,Lee JY,Song BK,Hahm DH,Song JK

更新日期：2011-04-01 00:00:00

abstract：:Vibrio parahaemolyticus is one of the major pathogens responsible for vibriosis and zoonotic infections in teleosts, marine invertebrates, and also humans through consumption of contaminated or unprocessed seafood. Emergence of resistance against current accessible antibiotics and spread to the food chain and environm...

journal_title：Applied microbiology and biotechnology

authors： Karan S,Choudhury D,Dixit A

更新日期：2021-01-01 00:00:00

abstract：:Corynebacterium glutamicum shows a great potential for the production of the polyamide monomer putrescine (1,4-diaminobutane). Previously, we constructed the putrescine-producing strain PUT1 by deletion of argF, the gene for ornithine transcarbamoylase (OTC), and argR, encoding the L-arginine repressor, combined with ...

journal_title：Applied microbiology and biotechnology

authors： Schneider J,Eberhardt D,Wendisch VF

更新日期：2012-07-01 00:00:00

abstract：:The transfer of plasmids was studied in a stirred fermentor in the course of mixed batch cultures combining recombinant strains of Lactococcus lactis subsp. lactis (donor strains) with L. lactis subsp. lactis CNRZ 268M3 (recipient strain). Donor strains contained one or two of the following plasmids (coding for erythr...

journal_title：Applied microbiology and biotechnology

authors： el Alami N,Boquien CY,Corrieu G

更新日期：1992-06-01 00:00:00

abstract：:The alphaproteobacterial Roseobacter clade (Rhodobacterales) is one of the most important global players in carbon and sulfur cycles of marine ecosystems. The remarkable metabolic versatility of this bacterial lineage provides access to diverse habitats and correlates with a multitude of extrachromosomal elements. Fou...

journal_title：Applied microbiology and biotechnology

authors： Petersen J,Frank O,Göker M,Pradella S

更新日期：2013-04-01 00:00:00

abstract：:Saccharomyces bayanus, being of interest for wine-making, is not as well known as S. cerevisiae and, due to many changes in the yeast classification, accurate data concerning its metabolic activity are difficult to find. In order to produce this yeast as an active dry yeast to be used as a starter in wine-making, its ...

journal_title：Applied microbiology and biotechnology

authors： Serra A,Strehaiano P,Taillandier P

更新日期：2003-10-01 00:00:00

abstract：:Bacterial cells are protected by an exoskeleton, the stabilizing and shape-maintaining cell wall, consisting of the complex macromolecule peptidoglycan. In view of its function, it could be assumed that the cell wall is a static structure. In truth, however, it is steadily broken down by peptidoglycan-cleaving enzymes...

journal_title：Applied microbiology and biotechnology

authors： Reith J,Mayer C

更新日期：2011-10-01 00:00:00

abstract：:α-Galacto-oligosaccharides (α-GOS) are produced by transgalactosylation reactions of α-galactosidase (α-Gal) or by conversion of raffinose family oligosaccharides by levansucrase. Similarly to β-GOS, α-GOS have the potential to mimic glycan receptors on eukaryotic cells and act as molecular decoys to prevent bacterial...

journal_title：Applied microbiology and biotechnology

authors： Wang Y,Black BA,Curtis JM,Gänzle MG

更新日期：2014-03-01 00:00:00

abstract：:Nanosized elemental sulfur (ES) is already reported to exert superior antimicrobial efficacy than micron-sized ES, which encourages their use in drugs and therapeutics. The aim of the present study is to explore the possible route and mode of antimicrobial action of orthorhombic (α-SNPs) and monoclinic (β-SNPs) allotr...

journal_title：Applied microbiology and biotechnology

authors： Roy Choudhury S,Mandal A,Ghosh M,Basu S,Chakravorty D,Goswami A

更新日期：2013-07-01 00:00:00

abstract：:The pathogenicity of Salmonella Typhimurium, a foodborne pathogen, is mainly attributed to its ability to form biofilm on food contact surfaces. ε-polylysine, a polymer of positively charged lysine, is reported to inhibit biofilm formation of both gram-positive and gram-negative bacteria. To elucidate the mechanism un...

journal_title：Applied microbiology and biotechnology

authors： Shen C,Islam MT,Masuda Y,Honjoh KI,Miyamoto T

更新日期：2020-06-01 00:00:00

abstract：:Acid tolerance and aroma profile are crucial factors for wine production in Saccharomyces cerevisiae. However, most wine yeasts to date fail to endure low-pH environments, therefore resulting in problems such as lengthened fermentation and poor flavor during acidic fruit wine production. In the present study, we estab...

journal_title：Applied microbiology and biotechnology

authors： Tian T,Wu D,Ng CT,Yang H,Sun J,Liu J,Lu J

更新日期：2020-04-01 00:00:00

abstract：:Immobilization of the enzyme benefits the catalytic industry a lot. The gram-positive enhancer matrix (GEM) particles could purify and immobilize the recombinant α-amylase in one step without changing the enzymatic character. The enzyme immobilized by GEM particles exhibited good reusability and storage stability. The...

journal_title：Applied microbiology and biotechnology

authors： Zhao F,Song Q,Wang B,Han Y,Zhou Z

更新日期：2020-01-01 00:00:00

abstract：:The cultivated and uncultivated bacterial communities of an activated sludge plant were studied. Two samples were taken and a total of 516 bacterial isolates were classified into groups using their whole-cell protein patterns. The distribution of bacteria into protein-pattern groups differed significantly between the ...

journal_title：Applied microbiology and biotechnology

authors： Vainio EJ,Moilanen A,Koivula TT,Bamford DH,Hantula J

更新日期：1997-07-01 00:00:00