Improved sandwich-hybridization assay for an electrical DNA-chip-based monitoring of bioprocess-relevant marker genes.

abstract：:Anode properties are critical for the performance of microbial electrolysis cells (MECs). In the present study, Fe nanoparticle-modified graphite disks were used as anodes to investigate the effects of nanoparticles on the performance of Shewanella oneidensis MR-1 in MECs. Results demonstrated that the average current...

journal_title：Applied microbiology and biotechnology

authors： Xu S,Liu H,Fan Y,Schaller R,Jiao J,Chaplen F

更新日期：2012-01-01 00:00:00

abstract：:The genetic improvement of winemaking yeasts is a virtually infinite process, as the design of new strains must always cope with varied and ever-evolving production contexts. Good wine yeasts must feature both good primary traits, which are related to the overall fermentative fitness of the strain, and secondary trait...

journal_title：Applied microbiology and biotechnology

authors： Bonciani T,De Vero L,Mezzetti F,Fay JC,Giudici P

更新日期：2018-03-01 00:00:00

abstract：:Streptomyces coelicolor "Müller" DSM3030 excretes a lysozyme comprising both beta-1,4-N-acetyl- and beta-1,4-N,6-O-diacetyl muramidase activities. The lysozyme is named Cellosyl. Gene libraries have been established using genomic DNA from the wild-type strain, S. coelicolor DSM3030, and from an overproducing mutant, S...

journal_title：Applied microbiology and biotechnology

authors： Bräu B,Hilgenfeld R,Schlingmann M,Marquardt R,Birr E,Wohlleben W,Aufderheide K,Pühler A

更新日期：1991-01-01 00:00:00

abstract：:Biotechnological production of vanillin is gaining momentum as the natural synthesis of vanillin that is very expensive. Ferulic acid (FA), a costly compound, is used as the substrate to produce vanillin biotechnologically and the making process is still expensive. Therefore, this study investigated the practical use ...

journal_title：Applied microbiology and biotechnology

authors： Chakraborty D,Selvam A,Kaur B,Wong JWC,Karthikeyan OP

更新日期：2017-07-01 00:00:00

abstract：:To understand the effect of daily bioaugmentation in full-scale grease interceptors (GIs), we compared the microbial communities occurring in two full-scale GIs during bioaugmented and non-bioaugmented cycles. The changes in microbial communities were determined using terminal restriction fragment length polymorphism ...

journal_title：Applied microbiology and biotechnology

authors： He X,So MJ,de Los Reyes FL 3rd

更新日期：2016-08-01 00:00:00

abstract：:Peroxidases (PRX, EC 1.11.1.7) are widely distributed across microorganisms, plants, and animals; and, in plants, they have been implicated in a variety of secondary metabolic reactions. In particular, horseradish (Armoracia rusticana) root represents the main source of commercial PRX production. The prxC1a gene, whic...

journal_title：Applied microbiology and biotechnology

authors： Matsui T,Nakayama H,Yoshida K,Shinmyo A

更新日期：2003-10-01 00:00:00

abstract：:Medicinal plants of the Dendrobium genus are highly prized, and hence, methodologies have been developed to authenticate Dendrobium drugs from its adulterants. Many bioactive constituents of Dendrobium species have been identified. The macromolecules included lectins; the enzymes chalcone synthase, sucrose synthase, a...

journal_title：Applied microbiology and biotechnology

authors： Ng TB,Liu J,Wong JH,Ye X,Wing Sze SC,Tong Y,Zhang KY

更新日期：2012-03-01 00:00:00

abstract：:Leavening ability in sweet dough is required for the commercial applications of baker's yeast. This property depends on many factors, such as glycolytic activity, sucrase activity, and osmotolerance. This study explored the importance of sucrase level on the leavening ability of baker's yeast in sweet dough. Furthermo...

journal_title：Applied microbiology and biotechnology

authors： Zhang CY,Lin X,Feng B,Liu XE,Bai XW,Xu J,Pi L,Xiao DG

更新日期：2016-07-01 00:00:00

abstract：:Due to efficient photosynthetic capability, robust growth, and clear genetic background, cyanobacteria are recently used for production of different biofuel and biochemical molecules by genetic engineering and showed great potentials as the next-generation microbial cell factory. For improving the production of bio-pr...

journal_title：Applied microbiology and biotechnology

authors： Tan X,Liang F,Cai K,Lu X

更新日期：2013-07-01 00:00:00

abstract：:The Gram-positive bacterium, Bacillus subtilis and related species are widely used industrially as hosts for producing enzymes. These species possess a high potential to produce secreted proteins into the culture medium. Nevertheless, the secretion of heterologous proteins by these species is frequently inefficient. I...

journal_title：Applied microbiology and biotechnology

authors： Kakeshita H,Kageyama Y,Ara K,Ozaki K,Nakamura K

更新日期：2011-03-01 00:00:00

abstract：:A beta-lactamase-free penicillin amidase from Alcaligenes sp. active against various beta-lactams was purified to homogeneity. The enzyme can hydrolyze penicillin G to 6-amino penicillanic acid (6-APA) and furnish penicillin G from 6-APA and phenyl acetic acid by condensation. The penicillin amidase is a heterodimer o...

journal_title：Applied microbiology and biotechnology

authors： Das S,Gayen JR,Pal A,Ghosh K,Rosazza JP,Samanta TB

更新日期：2004-08-01 00:00:00

abstract：:Bacillus stearothermophilus, a thermophilic bacterium isolated from the Kuwaiti desert, produced a variety of monohydroxy androstene derivatives and an oxidized product when incubated with exogenous testosterone for 24 h at 65 degrees C. The major metabolite was identified as androst-4-en-3,17-dione while minor metabo...

journal_title：Applied microbiology and biotechnology

authors： Al-Awadi S,Afzal M,Oommen S

更新日期：2003-07-01 00:00:00

abstract：:The lack of a universal method to extract RNA from soil hinders the progress of studies related to nitrification in soil, which is an important step in the nitrogen cycle. It is particularly difficult to extract RNA from certain types of soils such as Andosols (volcanic ash soils), which is the dominant agricultural s...

journal_title：Applied microbiology and biotechnology

authors： Wang Y,Nagaoka K,Hayatsu M,Sakai Y,Tago K,Asakawa S,Fujii T

更新日期：2012-11-01 00:00:00

abstract：:Aflatoxins are toxic and carcinogenic metabolites of several Aspergillus species. The effect of nitrate on aflatoxin production and expression of the key regulatory genes involved in aflatoxin biosynthesis, aflR and aflJ, were compared among isolates of the S(B) and S(BG) strains of A. flavus. Aflatoxin production by ...

journal_title：Applied microbiology and biotechnology

authors： Ehrlich KC,Cotty PJ

更新日期：2002-10-01 00:00:00

abstract：:Aliphatic ketones, such as 2-butanone and 3-hexanone, with only one carbon difference among side chains adjacent to the carbonyl carbon are difficult to be reduced enantioselectively. In this study, we utilized an acetophenone reductase from Geotrichum candidum NBRC 4597 (GcAPRD) to reduce challenging aliphatic ketone...

journal_title：Applied microbiology and biotechnology

authors： Koesoema AA,Sugiyama Y,Xu Z,Standley DM,Senda M,Senda T,Matsuda T

更新日期：2019-12-01 00:00:00

abstract：:Alternative ligands such as nucleic acid aptamers can be used for pathogen capture and detection and offer advantages over antibodies, including reduced cost, ease of production and modification, and improved stability. DNA aptamers demonstrating binding specificity to Salmonella enterica serovar Typhimurium were iden...

journal_title：Applied microbiology and biotechnology

authors： Dwivedi HP,Smiley RD,Jaykus LA

更新日期：2013-04-01 00:00:00

abstract：:Pneumocandin B0 is a hydrophobic secondary metabolite that accumulates in the mycelia of Glarea lozoyensis and inhibits fungal 1,3-β-glucan synthase. Extractive batch fermentation can promote the release of intracellular secondary metabolites into the fermentation broth and is often used in industry. The addition of e...

journal_title：Applied microbiology and biotechnology

authors： Yuan K,Huang B,Qin T,Song P,Zhang K,Ji X,Ren L,Zhang S,Huang H

更新日期：2019-08-01 00:00:00

abstract：:Corynebacterium glutamicum CCTCC M201005 produces a novel polygalacturonic acid bioflocculant, REA-11, consisting of galacturonic acid as the main structural unit. A biosynthetic pathway of REA-11 in C. glutamicum CCTCC M201005 was proposed. Evidence for the biosynthetic pathway was provided by: (1) analyzing the resp...

journal_title：Applied microbiology and biotechnology

authors： Li Y,He N,Guan H,Du G,Chen J

更新日期：2003-12-01 00:00:00

abstract：:Oxygenates, mainly methyl tert-butyl ether (MTBE), are commonly added to gasoline to enhance octane index and improve combustion efficiency. Other oxygenates used as gasoline additives are ethers such as ethyl tert-butyl ether (ETBE), tert-amyl methyl ether (TAME), and alcohols such as tert-butyl alcohol (TBA). As a r...

journal_title：Applied microbiology and biotechnology

authors： Fayolle F,Vandecasteele JP,Monot F

更新日期：2001-08-01 00:00:00

abstract：:Prenyltransferase NovQ is a vital class involved in the biosynthesis of secondary metabolites such as clorobiocin and novobiocin. To investigate the relationship between structure and catalytic properties of NovQ, here, we have analyzed the substrate-binding site, namely PT barrel, and revealed that menadione hydroqui...

journal_title：Applied microbiology and biotechnology

authors： Ni W,Zheng Z,Liu H,Wang P,Wang H,Sun X,Yang Q,Fang Z,Tang H,Zhao G

更新日期：2020-05-01 00:00:00

abstract：:In this study, the lipoxygenase (ana-LOX) gene from Anabaena sp. PCC 7120 was successful expressed and secreted in Bacillus subtilis. Under the control of the P43 promoter, with a signal peptide from the B. subtilis 168 nprB gene, and facilitated by the molecular chaperone PrsA, the production of the recombinant ana-L...

journal_title：Applied microbiology and biotechnology

authors： Zhang C,Tao T,Ying Q,Zhang D,Lu F,Bie X,Lu Z

更新日期：2012-05-01 00:00:00

abstract：:The inhibitory effects of aqueous, ethanolic and dichloromethane fractions from 16 marine algae from the Atlantic shores of North-East Brittany, France, have been investigated against microorganisms frequently associated with immersed surfaces. The extracts were tested in vitro against isolates of marine fungi, bacter...

journal_title：Applied microbiology and biotechnology

authors： Hellio C,Bremer G,Pons AM,Le Gal Y,Bourgougnon N

更新日期：2000-10-01 00:00:00

abstract：:Plants as well as pro- and eukaryotic microorganisms are able to synthesise lysine via de novo synthesis. While plants and bacteria, with some exceptions, rely on variations of the meso-diaminopimelate pathway for lysine biosynthesis, fungi exclusively use the α-aminoadipate pathway. Although bacteria and fungi are, i...

journal_title：Applied microbiology and biotechnology

authors： Fazius F,Zaehle C,Brock M

更新日期：2013-05-01 00:00:00

abstract：:The effects of pH control strategy and fermentative operation modes on the biosynthesis of pyrroloquinoline quinine (PQQ) were investigated systematically with Methylobacillus sp. CCTCC M2016079 in the present work. Firstly, the shake-flask cultivations and benchtop fermentations at various pH values ranging from 5.3 ...

journal_title：Applied microbiology and biotechnology

authors： Si Z,Machaku D,Wei P,Huang L,Cai J,Xu Z

更新日期：2017-06-01 00:00:00

abstract：:Hepatic lipid metabolic disorders and oxidative stress are involved in the development of non-alcoholic fatty liver disease (NAFLD). This study is to determine the protective effects of Lactobacillus plantarum NA136 on high-fat diet and fructose (HFD/F)-induced NAFLD and to elucidate its underlying molecular mechanism...

journal_title：Applied microbiology and biotechnology

authors： Zhao Z,Wang C,Zhang L,Zhao Y,Duan C,Zhang X,Gao L,Li S

更新日期：2019-07-01 00:00:00

abstract：:Forty-six pulp-bleaching fungi were screened for production of key enzymes for conversion of polychlorinated dibenzo-p-dioxins--lignin peroxidase (LiP), manganese peroxidase (MnP), and manganese-independent peroxidase (MiP)--under various conditions that would allow their utilization in the environment. Of 38 MnP-prod...

journal_title：Applied microbiology and biotechnology

authors： Manji S,Ishihara A

更新日期：2004-01-01 00:00:00

abstract：:Two hypothetical proteins XP_001818628 and XP_001819091 (designated AoFaeB and AoFaeC, respectively), showing sequence identity with known type-C feruloyl esterases, have been found in the genomic sequence of Aspergillus oryzae. We cloned the putative A. oryzae feruloyl esterase-encoding genes and expressed them in Pi...

journal_title：Applied microbiology and biotechnology

authors： Koseki T,Hori A,Seki S,Murayama T,Shiono Y

更新日期：2009-06-01 00:00:00

abstract：:Trichothecenes are sesquiterpene toxins produced by diverse but relatively few fungal species in at least three classes of Ascomycetes: Dothideomycetes, Eurotiomycetes, and Sordariomycetes. Approximately 200 structurally distinct trichothecene analogs have been described, but a given fungal species typically produces ...

journal_title：Applied microbiology and biotechnology

authors： Proctor RH,McCormick SP,Gutiérrez S

更新日期：2020-06-01 00:00:00

abstract：:The bacterial phosphoenolpyruvate (PEP)-dependent group translocation system (PTS) requires the presence of both membrane-bound and cytoplasmic components to phosphorylate and translocate sugar. Deinococcus radiodurans has a functional fruA gene coding for the membrane-bound components of the fructose-specific PTS. Ho...

journal_title：Applied microbiology and biotechnology

authors： Lee HY,Magotra M,Wong TY,Chakraborty C,Liu JK

更新日期：2012-02-01 00:00:00

abstract：:The essential general features required for lipid membranes of extremophilic archaea to fulfill biological functions are that they are in the liquid crystalline phase and have extremely low permeability of solutes that is much less temperature sensitive due to a lack of lipid-phase transition and highly branched isopr...

journal_title：Applied microbiology and biotechnology

authors： Ulrih NP,Gmajner D,Raspor P

更新日期：2009-08-01 00:00:00