Structural and physicochemical properties of polar lipids from thermophilic archaea.

abstract：:A cDNA of a structural gene encoding pyranose 2-oxidase (P2O) from Trametes ochracea strain MB49 was cloned into Escherichia coli strain BL21(DE3) on a multicopy plasmid under the control of the trc promoter. Synthesis of P2O was studied in batch cultures in LB or M9-based mineral medium at 28 degrees C. While there w...

journal_title：Applied microbiology and biotechnology

authors： Vecerek B,Maresová H,Kocanová M,Kyslík P

更新日期：2004-05-01 00:00:00

abstract：:A set of filamentous fungi (42 strains) was screened for alpha-N-acetylgalactosaminidase activity, and a series of inducers and different cultivation conditions were tested. Enzyme production by the best producer Aspergillus niger CCIM K2 was optimized and scaled up. alpha-N-Acetylgalactosaminidase was purified to app...

journal_title：Applied microbiology and biotechnology

authors： Weignerová L,Filipi T,Manglová D,Kren V

更新日期：2008-07-01 00:00:00

abstract：:Starting from mature vegetable compost, four bacterial strains were selected using a lignin-rich medium. 16S ribosomal RNA identification of the isolates showed high score similarity with Pseudomonas spp. for three out of four isolates. Further characterization of growth on mixtures of six selected lignin model compou...

journal_title：Applied microbiology and biotechnology

authors： Ravi K,García-Hidalgo J,Gorwa-Grauslund MF,Lidén G

更新日期：2017-06-01 00:00:00

abstract：:An endo-1,4-β-xylanase gene, xylcg, was cloned from Chaetomium globosum and successfully expressed in Escherichia coli. The complete gene of 675 bp was amplified, cloned into the pET 28(a) vector, and expressed. The optimal conditions for the highest activity of the purified recombinant XylCg were observed at a temper...

journal_title：Applied microbiology and biotechnology

authors： Singh RK,Tiwari MK,Kim D,Kang YC,Ramachandran P,Lee JK

更新日期：2013-08-01 00:00:00

abstract：:The removal of hydrophobic volatile organic compounds (VOCs) still remains the main restriction in the biological treatment of odorous emissions due to mass transfer limitations. The addition of a non-aqueous phase to conventional biotrickling filters (BTF) may overcome this limitation by enhancing VOCs transport from...

journal_title：Applied microbiology and biotechnology

authors： Lebrero R,Rodríguez E,Pérez R,García-Encina PA,Muñoz R

更新日期：2013-05-01 00:00:00

abstract：:Porcine circovirus type 3 (PCV3) is an emerging swine pathogen associated with acute porcine dermatitis and nephropathy syndrome (PDNS)-like clinical signs, reproductive failure, and multisystemic inflammation. Current evidence shows that PCV3 is spread worldwide, and its high incidence may pose a threat to the global...

journal_title：Applied microbiology and biotechnology

authors： Jiang M,Guo J,Zhang G,Jin Q,Liu Y,Jia R,Wang A

更新日期：2020-07-01 00:00:00

abstract：:The gene encoding a putative triphenylmethane reductase (TMR)-like protein derived from Geobacillus thermoglucosidasius C56-Y593 (named as GtAZR) was synthesized, heterologously expressed in Escherichia coli, and extensively characterized for the first time. The recombinant GtAZR displayed its maximum activity at pH 5...

journal_title：Applied microbiology and biotechnology

authors： Gao F,Ding H,Shao L,Xu X,Zhao Y

更新日期：2015-01-01 00:00:00

abstract：:Prenyltransferases of the dimethylallyltryptophan synthase (DMATS) superfamily are involved in the biosynthesis of secondary metabolites and show broad substrate specificity towards their aromatic substrates with a high regioselectivity for the prenylation reactions. Most members of this superfamily accepted as prenyl...

journal_title：Applied microbiology and biotechnology

authors： Pockrandt D,Sack C,Kosiol T,Li SM

更新日期：2014-06-01 00:00:00

abstract：:Clonorchis sinensis (C. sinensis), an important fishborne zoonotic parasite threatening public health, is of major socioeconomic importance in epidemic areas. Effective strategies are still urgently expected to prevent against C. sinensis infection. In the present study, paramyosin of C. sinensis (CsPmy) was stably an...

journal_title：Applied microbiology and biotechnology

authors： Sun H,Shang M,Tang Z,Jiang H,Dong H,Zhou X,Lin Z,Shi C,Ren P,Zhao L,Shi M,Zhou L,Pan H,Chang O,Li X,Huang Y,Yu X

更新日期：2020-02-01 00:00:00

abstract：:The novel influenza A virus (IAV) defective interfering particle "OP7" inhibits IAV replication in a co-infection and was previously suggested as a promising antiviral agent. Here, we report a batch-mode cell culture-based production process for OP7. In the present study, a seed virus containing standard virus (STV) a...

journal_title：Applied microbiology and biotechnology

authors： Hein MD,Kollmus H,Marichal-Gallardo P,Püttker S,Benndorf D,Genzel Y,Schughart K,Kupke SY,Reichl U

更新日期：2021-01-01 00:00:00

abstract：:The nucleoprotein of infectious hematopoietic necrosis virus (IHNV) is considered as the main target antigen for detection of IHNV infection in salmonid fish. This study aimed at improving the expression and solubility of IHNV nucleoprotein (IHNV-NP) in E. coli expression system. The effects of several expression stra...

journal_title：Applied microbiology and biotechnology

authors： Mohammadinezhad R,Farahmand H,Jalali SAH,Mirvaghefi A

更新日期：2018-05-01 00:00:00

abstract：:Dengue virus (DENV) and Japanese encephalitis virus (JEV) are closely related mosquito-borne flaviviruses. Together, they caused most arthropod-borne diseases in the world. Previously, we had demonstrated that both live attenuated and inactivated JE vaccines elicited cross-protection against DENV infection, and a DNA ...

journal_title：Applied microbiology and biotechnology

authors： Gao N,Li J,Sheng Z,Chen H,Fan D,Wang P,An J

更新日期：2019-06-01 00:00:00

abstract：:Mannosylerythritol lipids (MEL), which are abundantly secreted by yeasts, are one of the most promising biosurfactants known. To obtain various types of MEL and to attain a broad range of applications for them, screening of novel producers was undertaken. Thirteen strains of yeasts were successfully isolated as potent...

journal_title：Applied microbiology and biotechnology

authors： Konishi M,Morita T,Fukuoka T,Imura T,Kakugawa K,Kitamoto D

更新日期：2007-06-01 00:00:00

abstract：:The alpha-amylase of Streptomyces sp. IMD 2679 was subject to catabolite repression. Four different growth rates were achieved when the organism was grown at 40 degrees C and 55 degrees C in the presence and absence of cobalt, with an inverse relationship between alpha-amylase production and growth rate. Highest alpha...

journal_title：Applied microbiology and biotechnology

authors： Mc Mahon HE,Kelly CT,Fogarty WM

更新日期：1997-10-01 00:00:00

abstract：:D-Amino acids exist widely in microbes, plants, animals, and food and can be applied in pharmaceutical, food, and cosmetics. Because of their widespread applications in industry, D-amino acids have recently received more and more attention. Enzymes including D-hydantoinase, N-acyl-D-amino acid amidohydrolase, D-amino ...

journal_title：Applied microbiology and biotechnology

authors： Gao X,Ma Q,Zhu H

更新日期：2015-04-01 00:00:00

abstract：:Fluoroaromatics are widely and--in recent years--increasingly used as agrochemicals, starting materials for chemical syntheses and especially pharmaceuticals. This originates from the special properties the carbon-fluorine bond is imposing on organic molecules. Hence, fluoro-substituted compounds more and more are con...

journal_title：Applied microbiology and biotechnology

authors： Kiel M,Engesser KH

更新日期：2015-09-01 00:00:00

abstract：:Tolypocladium guangdongense is a high-value edible fungus with various medicinal and food safety properties. However, its evolutionary and genetic information is still limited. Mitochondrial genomes are potential models for molecular evolution and phylogenetic studies. In this study, we sequenced the complete mitogeno...

journal_title：Applied microbiology and biotechnology

authors： Zhang C,Dai Y,Wang G,Wang C,Gao Y,Deng W,Li T

更新日期：2020-11-01 00:00:00

abstract：:Aflatoxins are toxic and carcinogenic metabolites of several Aspergillus species. The effect of nitrate on aflatoxin production and expression of the key regulatory genes involved in aflatoxin biosynthesis, aflR and aflJ, were compared among isolates of the S(B) and S(BG) strains of A. flavus. Aflatoxin production by ...

journal_title：Applied microbiology and biotechnology

authors： Ehrlich KC,Cotty PJ

更新日期：2002-10-01 00:00:00

abstract：:The 454 pyrosequencing technique was applied to evaluate microbial community composition in sediment and water samples collected from the river receiving effluents from a swine farm and a farmhouse restaurant, respectively. For each sample, 4,600 effective sequences were selected and used to do the bacterial diversity...

journal_title：Applied microbiology and biotechnology

authors： Lu XM,Lu PZ

更新日期：2014-09-01 00:00:00

abstract：:The fungal secretome comprises various oxidative enzymes participating in the degradation of lignocellulosic biomass as a central step in carbon recycling. Among the secreted enzymes, aryl-alcohol oxidases (AAOs) are of interest for biotechnological applications including production of bio-based precursors for plastic...

journal_title：Applied microbiology and biotechnology

authors： Jankowski N,Koschorreck K,Urlacher VB

更新日期：2020-11-01 00:00:00

abstract：:Artificial cold adaptation of a mesophilic protease, subtilisin BPN' was attempted by means of random mutagenesis of its entire gene coupled with screening of cleared-zone-forming colonies on skim-milk plates at a low temperature. Out of sixty clones screened at 10 degrees C, one mutant enzyme (termed M-15) was found ...

journal_title：Applied microbiology and biotechnology

authors： Kano H,Taguchi S,Momose H

更新日期：1997-01-01 00:00:00

abstract：:We in this study describe a new method for genomic studies of individual uncultured prokaryotic organisms, which was used for the isolation and partial genome sequencing of a soil archaeon. The diversity of Archaea in a soil sample was mapped by generating a clone library using group-specific primers in combination wi...

journal_title：Applied microbiology and biotechnology

authors： Kvist T,Ahring BK,Lasken RS,Westermann P

更新日期：2007-03-01 00:00:00

abstract：:Grasslands in semi-arid Northern China are widely desertified, thus inducing the formation of a large area of moving sand lands. Revegetation of the sandy land is commonly adopted to restore degraded grasslands. The structure of the soil microbial community might dramatically change during degradation and recovery bec...

journal_title：Applied microbiology and biotechnology

authors： Cao C,Zhang Y,Cui Z,Feng S,Wang T,Ren Q

更新日期：2017-08-01 00:00:00

abstract：:Excess biomass buildup in biotrickling filters leads to low performance. The effect of biomass accumulation in a biotrickling filter (BTF) packed with polyurethane foam (PUF) was assessed in terms of hydrodynamics and void space availability in a system treating dimethyl disulfide (DMDS) vapors with an alkaliphilic co...

journal_title：Applied microbiology and biotechnology

authors： Arellano-García L,Dorado AD,Morales-Guadarrama A,Sacristan E,Gamisans X,Revah S

更新日期：2015-01-01 00:00:00

abstract：:Baicalin (baicalein 7-O-β-D-glucuronide) is one of the major flavonoid glucuronides found in traditional herbal medicines. Because its aglycone, baicalein, is absorbed more quickly and shows more effective properties than baicalin, the conversion of baicalin into baicalein by β-glucuronidase (GUS) has drawn the attent...

journal_title：Applied microbiology and biotechnology

authors： Sakurama H,Kishino S,Uchibori Y,Yonejima Y,Ashida H,Kita K,Takahashi S,Ogawa J

更新日期：2014-05-01 00:00:00

abstract：:D-Danshensu (D-DSS), a traditional Chinese medicine, is used to treat cardiovascular and cerebrovascular diseases. However, current isolation protocols for D-DSS both natural and synthetic are not ideal; therefore, in this study, we have developed a whole-cell biotransformation method to produce D-DSS from L-DOPA. Thi...

journal_title：Applied microbiology and biotechnology

authors： Xiong T,Jiang J,Bai Y,Fan TP,Zhao Y,Zheng X,Cai Y

更新日期：2019-08-01 00:00:00

abstract：:Superoxide dismutase (SOD) activity is one major defense line against oxidative stress for all of the aerobic organisms, and industrial production of this enzyme is highly demanded. The Cu/Zn superoxide dismutase gene (KmSOD1) of Kluyveromyces marxianus L3 was cloned and characterized. The deduced KmSod1p protein shar...

journal_title：Applied microbiology and biotechnology

authors： Raimondi S,Uccelletti D,Matteuzzi D,Pagnoni UM,Rossi M,Palleschi C

更新日期：2008-01-01 00:00:00

abstract：:A precise and scarless genome excision method, employing the Cre/loxP system in concert with double-strand break (DSB)-stimulated intramolecular recombination was developed. The DSBs were mediated by the restriction endonuclease, I-SceI. It permitted multiple deletions of independent 14-, 43-, and 10-kb-long genomic r...

journal_title：Applied microbiology and biotechnology

authors： Suzuki N,Nonaka H,Tsuge Y,Okayama S,Inui M,Yukawa H

更新日期：2005-11-01 00:00:00

abstract：:Under specific environmental conditions, Pseudomonas aeruginosa produces a biodegradable surfactant rhamnolipid. Evidences suggest that this biosurfactant is involved in protecting cells against oxidative stress; however, the effects of oxidative stress on its production and other virulence factors are still unclear. ...

journal_title：Applied microbiology and biotechnology

authors： Pacheco GJ,Reis RS,Fernandes AC,da Rocha SL,Pereira MD,Perales J,Freire DM

更新日期：2012-09-01 00:00:00

abstract：:Wood contains primary extractives, which are present in all woods, and secondary extractives, which are confined in certain wood species. Extractives in wood play a major role in wood-bonding processes, as they can contribute to or determine the bonding relevant properties of wood such as acidity and wettability. Ther...

journal_title：Applied microbiology and biotechnology

authors： Roffael E

更新日期：2016-02-01 00:00:00