Induction, purification and characterization of alpha-N-acetylgalactosaminidase from Aspergillus Niger.

abstract：:Human interleukin 6 (hIL-6) overproduced in Escherichia coli HB101 was found to partially retain the initiator methionine (Met) residue (Met-hIL-6). In order to remove the residual N-terminal Met in vivo, an attempt was made to express hIL-6 in aminopeptidase-P (Ap-P)-hyperproducing strains, since the N-terminus Met-P...

journal_title：Applied microbiology and biotechnology

authors： Yasueda H,Kikuchi Y,Kojima H,Nagase K

更新日期：1991-11-01 00:00:00

abstract：:Lantibiotic nukacin ISK-1 is produced by Staphylococcus warneri ISK-1. The dual functional transporter NukT, an ABC transporter maturation and secretion protein, contributes to cleavage of the leader peptide from the prepeptide (modified NukA) and the final transport of nukacin ISK-1. NukT consists of an N-terminal pe...

journal_title：Applied microbiology and biotechnology

authors： Zheng S,Nagao JI,Nishie M,Zendo T,Sonomoto K

更新日期：2018-01-01 00:00:00

abstract：:Dramatic increase in bacterial resistance towards conventional antibiotics emphasises the importance to identify novel, more potent antimicrobial therapies. Antimicrobial peptides (AMPs) have emerged as a promising new group to be evaluated in therapeutic intervention of infectious diseases. Here we describe a novel A...

journal_title：Applied microbiology and biotechnology

authors： Myhrman E,Håkansson J,Lindgren K,Björn C,Sjöstrand V,Mahlapuu M

更新日期：2013-04-01 00:00:00

abstract：:The environmentally widespread micropollutants bisphenol A (BPA), carbamazepine (CBZ), 17α-ethinylestradiol (EE2), diclofenac (DF), sulfamethoxazole (SMX), technical nonylphenol (t-NP) and triclosan (TCS) were used to assess the potential of the laccase-producing freshwater ascomycete Phoma sp. strain UHH 5-1-03 for m...

journal_title：Applied microbiology and biotechnology

authors： Hofmann U,Schlosser D

更新日期：2016-03-01 00:00:00

abstract：:The original version of this article was revised: After publication of this article, the publisher was notified that Michael Klocke has been listed as an author without his consent. ...

journal_title：Applied microbiology and biotechnology

authors： Carabeo-Pérez A,Guerra-Rivera G,Ramos-Leal M,Jiménez-Hernández J

更新日期：2019-12-01 00:00:00

abstract：:It is well established that the choice of yeast used to perform wine fermentation significantly influences the sensory attributes of wines; different yeast species and strains impart different profiles of esters, volatile fatty acids, higher alcohols, and volatile sulphur compounds. Indeed, choice of yeast remains one...

journal_title：Applied microbiology and biotechnology

authors： Cordente AG,Solomon M,Schulkin A,Leigh Francis I,Barker A,Borneman AR,Curtin CD

更新日期：2018-07-01 00:00:00

abstract：:Phaffia rhodozyma (now Xanthophyllomyces dendrorhous) and Haematococcus pluvialis are known as the major prominent microorganisms able to synthesize astaxanthin natural pigment. Important research efforts have been made to determine optimal conditions for astaxanthin synthesis. When the focus is on astaxanthin product...

journal_title：Applied microbiology and biotechnology

authors： Domínguez-Bocanegra AR,Ponce-Noyola T,Torres-Muñoz JA

更新日期：2007-06-01 00:00:00

abstract：:1,3-propanediol (1,3-PD) was produced with a robust fermentation process using waste glycerol feedstock from biodiesel production and a soil-based bacterial inoculum. An iterative inoculation method was developed to achieve independence from soil and selectively breed bacterial populations capable of glycerol metaboli...

journal_title：Applied microbiology and biotechnology

authors： Kanjilal B,Noshadi I,Bautista EJ,Srivastava R,Parnas RS

更新日期：2015-03-01 00:00:00

abstract：:Environmentally friendly control measures are needed for suppression of soilborne pathogens of vegetable crops in the Republic of Korea. In vitro challenge assays were used to screen approximately 500 bacterial isolates from 20 Korean greenhouse soils for inhibition of diverse plant pathogens. One isolate, Bacillus su...

journal_title：Applied microbiology and biotechnology

authors： Chung S,Kong H,Buyer JS,Lakshman DK,Lydon J,Kim SD,Roberts DP

更新日期：2008-08-01 00:00:00

abstract：:Transaminase enzymes (TAms) are becoming increasingly valuable in the chemist's toolbox as a biocatalytic route to chiral amines. Despite high profile successes, the lack of (R)-selective TAms and robustness under harsh industrial conditions continue to prove problematic. Herein, we report the isolation of the first h...

journal_title：Applied microbiology and biotechnology

authors： Kelly SA,Magill DJ,Megaw J,Skvortsov T,Allers T,McGrath JW,Allen CCR,Moody TS,Gilmore BF

更新日期：2019-07-01 00:00:00

abstract：:We in this study describe a new method for genomic studies of individual uncultured prokaryotic organisms, which was used for the isolation and partial genome sequencing of a soil archaeon. The diversity of Archaea in a soil sample was mapped by generating a clone library using group-specific primers in combination wi...

journal_title：Applied microbiology and biotechnology

authors： Kvist T,Ahring BK,Lasken RS,Westermann P

更新日期：2007-03-01 00:00:00

abstract：:Transfer of a biosynthetic pathway between evolutionary distant organisms can create a metabolic shunt capable of bypassing the native regulation of the host organism, hereby improving the production of secondary metabolite precursor molecules for important natural products. Here, we report the engineering of Escheric...

journal_title：Applied microbiology and biotechnology

authors： Carlsen S,Ajikumar PK,Formenti LR,Zhou K,Phon TH,Nielsen ML,Lantz AE,Kielland-Brandt MC,Stephanopoulos G

更新日期：2013-07-01 00:00:00

abstract：:This study demonstrated for the first time the possibility to remove and partially recover the Ruthenium contained in industrial effluents by using purple non sulfur bacteria (PNSB) as microbial biosorbents. Up to date, the biosorption was only claimed as possible tool for the removal of the platinum-group metals (PGM...

journal_title：Applied microbiology and biotechnology

authors： Colica G,Caparrotta S,De Philippis R

更新日期：2012-07-01 00:00:00

abstract：:Streptomyces avermitilis is well known as the producer of anthelmintic agent avermectins, which are widely used in agriculture, veterinary medicine, and human medicine. aveI encodes a TetR-family regulator, which is the homolog of AtrA. It was reported that deletion of aveI caused enhanced avermectin production. In th...

journal_title：Applied microbiology and biotechnology

authors： Liu L,Cheng Y,Lyu M,Zhao X,Wen Y,Li J,Chen Z

更新日期：2019-10-01 00:00:00

abstract：:Both varied and strong promoters are essential for metabolic and pathway engineering applications in any host organism. To enable this capacity, here we demonstrate a generalizable method for the de novo construction of strong, synthetic hybrid promoter libraries. Specifically, we demonstrate how promoter truncation a...

journal_title：Applied microbiology and biotechnology

authors： Blazeck J,Reed B,Garg R,Gerstner R,Pan A,Agarwala V,Alper HS

更新日期：2013-04-01 00:00:00

abstract：:Exophiala jeanselmei is an opportunistic pathogenic black yeast growing in humid environments such as water reservoirs of air-conditioning systems. Because this fungal contaminant could be vaporized into the air and subsequently cause health problems, its monitoring is recommended. Currently, this monitoring is based ...

journal_title：Applied microbiology and biotechnology

authors： Libert X,Chasseur C,Packeu A,Bureau F,Roosens NH,De Keersmaecker SJC

更新日期：2016-02-01 00:00:00

abstract：:Crude cell extracts of Pseudomonas putida F6 transformed 4-substituted fluoro-, chloro-, bromo- and iodo-phenol without the exogenous addition of cofactors. The rate of substrate consumption decreased with increasing substituent size (F>Cl>Br>I). Biotransformations resulted in greater than 95% utilisation of the halog...

journal_title：Applied microbiology and biotechnology

authors： Brooks SJ,Doyle EM,Hewage C,Malthouse JP,Duetz W,O' Connor KE

更新日期：2004-05-01 00:00:00

abstract：:5-Hydroxytryptophan (5-HTP) is the precursor of the neurotransmitter serotonin and has been used for the treatment of various diseases such as depression, insomnia, chronic headaches, and binge eating associated obesity. The production of 5-HTP had been achieved in our previous report, by the development of a recombin...

journal_title：Applied microbiology and biotechnology

authors： Xu D,Fang M,Wang H,Huang L,Xu Q,Xu Z

更新日期：2020-03-01 00:00:00

abstract：:Although yeasts are known to be able to incorporate a wide variety of exogenous sterols under strict anaerobiosis, no data are available on the assimilation of grapevine phytosterols under enological conditions and the eventual impact on fermentation kinetics. We used therefore a mixture of pure phytosterols, in a pro...

journal_title：Applied microbiology and biotechnology

authors： Luparia V,Soubeyrand V,Berges T,Julien A,Salmon JM

更新日期：2004-07-01 00:00:00

abstract：:The 16S ribosomal RNA (rRNA) gene has been the most commonly used sequence to characterize bacterial communities. The classical approach to obtain gene sequences to study bacterial diversity implies cloning amplicons, selecting clones, and Sanger sequencing cloned fragments. A more recent approach is direct sequencing...

journal_title：Applied microbiology and biotechnology

authors： Assunção A,Costa MC,Carlier JD

更新日期：2016-03-01 00:00:00

abstract：:The desire to reduce dependence on the ever diminishing fossil fuel reserves coupled with the impetus towards green energy has seen increased research in biofuels as alternative sources of energy. Lignocellulose materials are one of the most promising feedstocks for advanced biofuels production. However, their utilisa...

journal_title：Applied microbiology and biotechnology

authors： Kudanga T,Le Roes-Hill M

更新日期：2014-08-01 00:00:00

abstract：:The helix-loop-helix (HLH) family of transcriptional factors is a key player in a wide range of developmental processes in organisms from mammals to microbes. We previously identified the bHLH transcription factor SclR in Aspergillus oryzae and found that the loss of SclR function led to significant phenotypic changes...

journal_title：Applied microbiology and biotechnology

authors： Jin FJ,Han P,Zhuang M,Zhang ZM,Jin L,Koyama Y

更新日期：2018-01-01 00:00:00

abstract：:The merC gene from the Tn21-encoded mer operon has potential uses as a molecular tool for bioremediation. It was overexpressed as the fusion proteins MerC-Sso1p or MerC-Vam3p in Saccharomyces cerevisiae. Green fluorescent protein (GFP)-MerC-Sso1p fusion proteins located primarily in the plasma membrane, although some ...

journal_title：Applied microbiology and biotechnology

authors： Kiyono M,Miyahara K,Sone Y,Pan-Hou H,Uraguchi S,Nakamura R,Sakabe K

更新日期：2010-03-01 00:00:00

abstract：:Red koji or red mold rice is prepared by growing a genus Monascus on steamed rice. For centuries, it has been used in Asia for the production of fermented foods including red rice wine and fermented tofu. Although red koji is an important source of various hydrolytic enzymes critical for food fermentation, information...

journal_title：Applied microbiology and biotechnology

authors： Yasuda M,Tachibana S,Kuba-Miyara M

更新日期：2012-10-01 00:00:00

abstract：:L-Ribose is a rare and expensive sugar that can be used as a precursor for the production of L-nucleoside analogues, which are used as antiviral drugs. In this work, we describe a novel way of producing L-ribose from the readily available raw material L-arabinose. This was achieved by introducing L-ribose isomerase ac...

journal_title：Applied microbiology and biotechnology

authors： Helanto M,Kiviharju K,Granström T,Leisola M,Nyyssölä A

更新日期：2009-05-01 00:00:00

abstract：:The medicinal mushroom Agaricus blazei produced high amounts of laccase (up to 5,000 units l(-1)) in a complex, agitated liquid medium based on tomato juice, while only traces of the enzyme (<100 units l(-1)) were detected in synthetic glucose-based medium. Purification of the enzyme required three chromatographic ste...

journal_title：Applied microbiology and biotechnology

authors： Ullrich R,Huong le M,Dung NL,Hofrichter M

更新日期：2005-05-01 00:00:00

abstract：:Biofilms in drinking water distribution systems (DWDSs) could cause several types of problems, such as the deterioration of water quality, corrosion of pipe walls, and potential proliferation of opportunistic pathogens. In this study, ten biofilm samples from different pipe materials, including ductile cast iron pipe ...

journal_title：Applied microbiology and biotechnology

authors： Ren H,Wang W,Liu Y,Liu S,Lou L,Cheng D,He X,Zhou X,Qiu S,Fu L,Liu J,Hu B

更新日期：2015-12-01 00:00:00

abstract：:In this work, phytosterol-biotransforming strains were selected from Mycobacterium sp., using a high concentration of beta-sitosterol. The selection was made by culturing the strains in a medium enriched with 14 g beta-sitosterol/l as the unique source of carbon. During 2 months, the bacterial cultures were transferre...

journal_title：Applied microbiology and biotechnology

authors： Vidal M,Becerra J,Mondaca MA,Silva M

更新日期：2001-10-01 00:00:00

abstract：:Although a high number of chickens carry Campylobacter jejuni, the mechanistic action of colonization in the intestine is still poorly understood. The current study was therefore designed to investigate the effects of C. jejuni on glucose uptake, amino acids availability in digesta, and intracellular calcium [Ca(2+)]i...

journal_title：Applied microbiology and biotechnology

authors： Awad WA,Smorodchenko A,Hess C,Aschenbach JR,Molnár A,Dublecz K,Khayal B,Pohl EE,Hess M

更新日期：2015-08-01 00:00:00

abstract：:Escherichia coli cells expressing mink (Mustela vison) growth hormone were grown in a batch fermentation process. The expression level was estimated to be 27% of the total cellular protein after 3 h of induction with 1 mM isopropyl beta-D-thiogalactoside (IPTG). If the expression of mink growth hormone (mGH) was induc...

journal_title：Applied microbiology and biotechnology

authors： Sereikaite J,Statkute A,Morkunas M,Radzevicius K,Borromeo V,Secchi C,Bumelis VA

更新日期：2007-02-01 00:00:00