Specific single-cell isolation and genomic amplification of uncultured microorganisms.

abstract：:Enterokinase is one of the most frequently used enzymes for the removal of affinity tags from target recombinant proteins. In this study, several fermentation strategies were assayed for the production of human enterokinase in Pichia pastoris under constitutive GAP promoter. Two of them with controlled specific growth...

journal_title：Applied microbiology and biotechnology

authors： Melicherová K,Krahulec J,Šafránek M,Lišková V,Hopková D,Széliová D,Turňa J

更新日期：2017-03-01 00:00:00

abstract：:There is a growing demand for silver-based biocides, including both ionic silver forms and metallic nanosilver. The use of metallic nanosilver, typically chemically produced, faces challenges including particle agglomeration, high costs, and upscaling difficulties . Additionally, there exists a need for the developmen...

journal_title：Applied microbiology and biotechnology

authors： Sintubin L,De Windt W,Dick J,Mast J,van der Ha D,Verstraete W,Boon N

更新日期：2009-09-01 00:00:00

abstract：:High vanillin productivity was achieved in the batch biotransformation of ferulic acid by Streptomyces sp. strain V-1. Due to the toxicity of vanillin and the product inhibition, fed-batch biotransformation with high concentration of ferulic acid was unsuccessful. To solve this problem and improve the vanillin yield, ...

journal_title：Applied microbiology and biotechnology

authors： Hua D,Ma C,Song L,Lin S,Zhang Z,Deng Z,Xu P

更新日期：2007-03-01 00:00:00

abstract：:Trichothecenes are sesquiterpene toxins produced by diverse but relatively few fungal species in at least three classes of Ascomycetes: Dothideomycetes, Eurotiomycetes, and Sordariomycetes. Approximately 200 structurally distinct trichothecene analogs have been described, but a given fungal species typically produces ...

journal_title：Applied microbiology and biotechnology

authors： Proctor RH,McCormick SP,Gutiérrez S

更新日期：2020-06-01 00:00:00

abstract：:Penicillin G acylase from Achromobacter sp. (NPGA) was studied in the enzymatic synthesis of β-lactam antibiotics by kinetically controlled N-acylation. When compared with penicillin acylase of Escherichia coli (PGA), the NPGA was significantly more efficient at syntheses of ampicillin and amoxicillin (higher S/H rati...

journal_title：Applied microbiology and biotechnology

authors： Bečka S,Štěpánek V,Vyasarayani RW,Grulich M,Maršálek J,Plháčková K,Dobišová M,Marešová H,Plačková M,Valešová R,Palyzová A,Datla A,Ashar TK,Kyslík P

更新日期：2014-02-01 00:00:00

abstract：:A purification procedure for an extracellular alpha-amylase from Streptomyces rimosus, oxytetracycline-producing strain, is described. The enzyme obtained was shown to be an acidic (pI 4.75) monomer with a relative molecular mass (M(r)) of 43,000, containing three cysteines involved in the catalytic activity of the en...

journal_title：Applied microbiology and biotechnology

authors： Vukelić B,Ritonja A,Renko M,Pokorny M,Vitale L

更新日期：1992-05-01 00:00:00

abstract：:Determining spatiotemporal gene expression and analyzing knockout mutant phenotypes have become powerful tools in elucidating the function of genes; however, genetic approaches for simultaneously inactivating a gene and monitoring its expression have not been reported in the literature. In this study, we designed a du...

journal_title：Applied microbiology and biotechnology

authors： Chen K,Xu X,Gu L,Hildreth M,Zhou R

更新日期：2015-02-01 00:00:00

abstract：:Magnetic carbon nanotubes (MCNTs) with necklace-like nanostructures was prepared via hydrothermal method, and hyperbranched poly(amidoamine) (PAMAM) was grafted on the surface of MCNTs on the basis of the Michael addition of methyl acrylate and the amidation of the resulting ester with a large excess of ethylenediamin...

journal_title：Applied microbiology and biotechnology

authors： Zhao G,Li Y,Wang J,Zhu H

更新日期：2011-08-01 00:00:00

abstract：:Although a high number of chickens carry Campylobacter jejuni, the mechanistic action of colonization in the intestine is still poorly understood. The current study was therefore designed to investigate the effects of C. jejuni on glucose uptake, amino acids availability in digesta, and intracellular calcium [Ca(2+)]i...

journal_title：Applied microbiology and biotechnology

authors： Awad WA,Smorodchenko A,Hess C,Aschenbach JR,Molnár A,Dublecz K,Khayal B,Pohl EE,Hess M

更新日期：2015-08-01 00:00:00

abstract：:Shikimic acid is an industrially important chiral compound used as a key ingredient in formulation of drug Oseltamivir phosphate (Tamiflu) for the treatment of swine/avian flu. The high cost and limited availability of shikimic acid isolated from plants has detained the use of this valuable building block of the drug....

journal_title：Applied microbiology and biotechnology

authors： Rawat G,Tripathi P,Saxena RK

更新日期：2013-05-01 00:00:00

abstract：:Respiration measurement in shake flasks is introduced as a new method to characterize the metabolic activity of microorganisms during and after stress exposure. The major advantage of the new method is the possibility to determine the metabolic activity independent of manual sampling without the necessity to change th...

journal_title：Applied microbiology and biotechnology

authors： Seletzky JM,Noack U,Fricke J,Hahn S,Büchs J

更新日期：2006-10-01 00:00:00

abstract：:The medicinal mushroom Agaricus blazei produced high amounts of laccase (up to 5,000 units l(-1)) in a complex, agitated liquid medium based on tomato juice, while only traces of the enzyme (<100 units l(-1)) were detected in synthetic glucose-based medium. Purification of the enzyme required three chromatographic ste...

journal_title：Applied microbiology and biotechnology

authors： Ullrich R,Huong le M,Dung NL,Hofrichter M

更新日期：2005-05-01 00:00:00

abstract：:Inhibitory compounds liberated from lignocellulose pretreatment are representative toxic chemicals that repress microbial growth and metabolism. A tolerant strain of the industrial yeast Saccharomyces cerevisiae is able to detoxify a major class of toxic compounds while producing ethanol. Knowledge on the yeast tolera...

journal_title：Applied microbiology and biotechnology

authors： Liu ZL,Huang X,Zhou Q,Xu J

更新日期：2019-07-01 00:00:00

abstract：:The sheep rumen microbial community plays an important role in animal performance and the environment. Few studies have paid close attention to the impact of different levels of dietary nutrition on rumen microbial populations. A total of 112 healthy Tan sheep were selected and randomly allotted to one of four dietary...

journal_title：Applied microbiology and biotechnology

authors： Wang Y,Cao P,Wang L,Zhao Z,Chen Y,Yang Y

更新日期：2017-05-01 00:00:00

abstract：:The construction of a new phagemid vector for display of peptides on the pVIII major coat protein of filamentous bacteriophage is described, in which expression of pVIII-peptide fusions was placed under the control of the arabinose-inducible P(BAD) promoter. The new phagemid showed excellent capacity for the regulatio...

journal_title：Applied microbiology and biotechnology

authors： Fagerlund A,Myrset AH,Kulseth MA

更新日期：2008-10-01 00:00:00

abstract：:The carbon and nitrogen sources most suitable for L-asparaginase production by Enterobacter aerogenes were selected and their concentrations optimized in shake-flask cultures. Sodium citrate (1.0%) and diammonium hydrogen phosphate (0.16%) proved to be the best sources of carbon and nitrogen, respectively. Nitrogen ca...

journal_title：Applied microbiology and biotechnology

authors： Mukherjee J,Majumdar S,Scheper T

更新日期：2000-02-01 00:00:00

abstract：:Biosurfactant rhamnolipids have been claimed to show biological activities of inhibiting the proliferation of cancer cells. In this study, the cytotoxicity of rhamnolipids was examined on four cancer cells (HepG2, Caco-2, Hela, MCF-7 cells) and two normal cells (HK-2 cell, primary hepatocyte). Interestingly, both canc...

journal_title：Applied microbiology and biotechnology

authors： Jiang L,Shen C,Long X,Zhang G,Meng Q

更新日期：2014-12-01 00:00:00

abstract：:Cellobiose dehydrogenases (CDHs) are extracellular glycosylated haemoflavoenzymes produced by many different wood-degrading and phytopathogenic fungi. Putative cellobiose dehydrogenase genes are recurrently discovered by genome sequencing projects in various phylogenetically distinct fungi. The genomes from the basidi...

journal_title：Applied microbiology and biotechnology

authors： Turbe-Doan A,Arfi Y,Record E,Estrada-Alvarado I,Levasseur A

更新日期：2013-06-01 00:00:00

abstract：:In this study, we investigated the use of Illumina high-throughput sequencing of 16S ribosomal RNA (rRNA) amplicons to explore microbial diversity and community structure in raw and secondary treated wastewater (WW) samples from four municipal wastewater treatment plants (WWTPs A-D) across Australia. Sequence reads we...

journal_title：Applied microbiology and biotechnology

authors： Ahmed W,Staley C,Sidhu J,Sadowsky M,Toze S

更新日期：2017-02-01 00:00:00

abstract：:A key factor contributing to the variability in the microbial kinetic parameters reported from batch assays is parameter identifiability, i.e., the ability of the mathematical routine used for parameter estimation to provide unique estimates of the individual parameter values. This work encompassed a three-part evalua...

journal_title：Applied microbiology and biotechnology

authors： Seagren EA,Kim H,Smets BF

更新日期：2003-05-01 00:00:00

abstract：:A 6-kDa antifungal peptide with inhibitory activity on mycelial growth in Fusarium oxysporum, Mycosphaerella arachidicola, and Physalospora piricola was isolated from baby lima beans. The peptide suppressed growth in M. arachidicola with an IC(50) of 0.87 muM and inhibited activity of HIV-1 reverse transcriptase with ...

journal_title：Applied microbiology and biotechnology

authors： Wang HX,Ng TB

更新日期：2006-12-01 00:00:00

abstract：:Grapevines are sensitive to a wide range of fungal pathogens, including agents such as Phaeomoniella chlamydospora and Phaeoacremonium aleophilum that cause tracheomycosis. In the present study, a procedure for DNA extraction from grapevine woody tissue is first evaluated and shown to be suitable for quantitative anal...

journal_title：Applied microbiology and biotechnology

authors： Pouzoulet J,Mailhac N,Couderc C,Besson X,Daydé J,Lummerzheim M,Jacques A

更新日期：2013-12-01 00:00:00

abstract：:The aim of this study was to determine the antimicrobial effects of lemongrass essential oil (C. flexuosus) and to determine cytotoxic effects of both test compounds on human dermal fibroblasts. Antimicrobial susceptibility screening was carried out using the disk diffusion method. Antimicrobial resistance was observe...

journal_title：Applied microbiology and biotechnology

authors： Adukwu EC,Bowles M,Edwards-Jones V,Bone H

更新日期：2016-11-01 00:00:00

abstract：:The lysin gene (lysA2) of the Lactobacillus casei bacteriophage A2 was cloned and expressed in Escherichia coli. LysA2 is an endopeptidase that hydrolyzes the bond between the terminal D: -alanine of the peptidoglycan tetrapeptide and the aspartic acid residue that forms the bridge with the L: -lysine of a neighboring...

journal_title：Applied microbiology and biotechnology

authors： Ribelles P,Rodríguez I,Suárez JE

更新日期：2012-04-01 00:00:00

abstract：:For ethical, technical, regulatory, and cost reasons, in vitro methods are increasingly used as an alternative to in vivo experimentations. The aim of the present study was to validate, according to in vivo data in living animals, a new in vitro model of the piglet colon, the PigutIVM, under both control conditions an...

journal_title：Applied microbiology and biotechnology

authors： Fleury MA,Le Goff O,Denis S,Chaucheyras-Durand F,Jouy E,Kempf I,Alric M,Blanquet-Diot S

更新日期：2017-03-01 00:00:00

abstract：:Robustness of fermenting strains to lignocellulose derived inhibitors is critical for efficient biofuel and biochemical productions. In this study, the industrial fermenting strain Corynebacterium glutamicum S9114 was evolved for improved inhibitor tolerance using long-term adaptive evolution by continuously transferr...

journal_title：Applied microbiology and biotechnology

authors： Wang X,Khushk I,Xiao Y,Gao Q,Bao J

更新日期：2018-01-01 00:00:00

abstract：:The causes of Bacillus spore resistance remain unclear. Many structures including a highly compact envelope, low hydration of the protoplast, high concentrations of Ca-chelated dipicolinic acid, and the presence of small acid-soluble spore proteins seem to contribute to resistance. To evaluate the role of internal pro...

journal_title：Applied microbiology and biotechnology

authors： Nguyen Thi Minh H,Guyot S,Perrier-Cornet JM,Gervais P

更新日期：2008-08-01 00:00:00

abstract：:Escherichia coli and Lactobacillus plantarum were subjected to final water potentials of -5.6 MPa and -11.5 MPa with three solutes: glycerol, sorbitol and NaCl. The water potential decrease was realized either rapidly (osmotic shock) or slowly (20 min) and a difference in cell viability between these conditions was on...

journal_title：Applied microbiology and biotechnology

authors： Poirier I,Maréchal PA,Evrard C,Gervais P

更新日期：1998-12-01 00:00:00

abstract：:The alpha-amylase of Streptomyces sp. IMD 2679 was subject to catabolite repression. Four different growth rates were achieved when the organism was grown at 40 degrees C and 55 degrees C in the presence and absence of cobalt, with an inverse relationship between alpha-amylase production and growth rate. Highest alpha...

journal_title：Applied microbiology and biotechnology

authors： Mc Mahon HE,Kelly CT,Fogarty WM

更新日期：1997-10-01 00:00:00

abstract：:Some strains belonging to the genera Citrobacter and Enterobacter have been reported to produce chitin/chitosan-like bioflocculants (BFs) from acetate. In this study, to investigate the distribution of the BF-producing potential in the genus Citrobacter and to screen stably and highly BF-producing strains, we obtained...

journal_title：Applied microbiology and biotechnology

authors： Kimura K,Inoue T,Kato D,Negoro S,Ike M,Takeo M

更新日期：2013-11-01 00:00:00