当前位置： SCI文献检索 > APPLIED MICROBIOLOGY AND BIOTECHNOLOGY期刊下所有文献 > Optimization of the fermentation and downstream processes for human enterokinase production in Pichia pastoris.

Optimization of the fermentation and downstream processes for human enterokinase production in Pichia pastoris.

Abstract：

:Enterokinase is one of the most frequently used enzymes for the removal of affinity tags from target recombinant proteins. In this study, several fermentation strategies were assayed for the production of human enterokinase in Pichia pastoris under constitutive GAP promoter. Two of them with controlled specific growth rate during whole cultivation showed a very low enterokinase activity, under 1 U/ml, of the fermentation medium. On the contrary, the combined fermentation with a maximum specific growth rate at the initial phase of the fermentation and stationary-like phase during the rest of the fermentation showed a significant accumulation of the enterokinase in the medium, which counted up to 1400 U/ml. Lower cultivation temperature had a negative impact on the enzyme accumulation during this fermentation strategy. Downstream processes were focused on buffer environment optimization directly after cultivation, as at this time, the most amount of the activity is eliminated by endogenous proteases. Slightly positive effect on enzyme activity in the medium had an addition of liquid storage solution of EDTA and KOH to adjust pH to 8 and molarity of the EDTA to 50 mM. During the purification process, a significant amount of the enzyme was detected to be lost, which counted up to 90%. The purified enzyme, enterokinase, kept quality standard of the published enzymes.

journal_name

Appl Microbiol Biotechnol

journal_title

Applied microbiology and biotechnology

authors

Melicherová K,Krahulec J,Šafránek M,Lišková V,Hopková D,Széliová D,Turňa J

doi

10.1007/s00253-016-7960-3

subject

Has Abstract

pub_date

2017-03-01 00:00:00

pages

1927-1934

issue

5

eissn

0175-7598

issn

1432-0614

pii

10.1007/s00253-016-7960-3

journal_volume

101

pub_type

杂志文章

相关文献

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY文献大全
  • Metabolic engineering of flavonoids in plants and microorganisms.

    abstract：:Over 9,000 flavonoid compounds have been found in various plants, comprising one of the largest families of natural products. Flavonoids are an essential factor in plant interactions with the environment, often serving as the first line of defense against UV irradiation and pathogen attacks. Flavonoids are also major ...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章,评审

    doi：10.1007/s00253-011-3449-2

    authors： Wang Y,Chen S,Yu O

    更新日期：2011-08-01 00:00:00

  • Bacillus and Paenibacillus secreted polyketides and peptides involved in controlling human and plant pathogens.

    abstract：:Overuse of broad-spectrum antibiotics to control human and plant pathogens greatly accelerated the development of antibiotic resistance among bacteria and fungi. Therefore, usage of new approaches is necessary to control outbreaks of phytopathogenic diseases as well as multidrug-resistant human pathogens. Many of the ...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章,评审

    doi：10.1007/s00253-018-9541-0

    authors： Olishevska S,Nickzad A,Déziel E

    更新日期：2019-02-01 00:00:00

  • Use of the Aspergillus oryzae actin gene promoter in a novel reporter system for exploring antifungal compounds and their target genes.

    abstract：:Demand for novel antifungal drugs for medical and agricultural uses has been increasing because of the diversity of pathogenic fungi and the emergence of drug-resistant strains. Genomic resources for various living species, including pathogenic fungi, can be utilized to develop novel and effective antifungal compounds...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-010-2627-y

    authors： Marui J,Yoshimi A,Hagiwara D,Fujii-Watanabe Y,Oda K,Koike H,Tamano K,Ishii T,Sano M,Machida M,Abe K

    更新日期：2010-08-01 00:00:00

  • Differential effects of bioreactor process variables and purification on the human recombinant lysosomal enzyme β-glucuronidase produced from Chinese hamster ovary cells.

    abstract：:β-Glucuronidase is a lysosomal enzyme and a molecular model of a class of therapeutics approved as enzyme replacement therapies for lysosomal storage diseases. Understanding the effect of bioreactor process variables on the production and quality of the biologics is critical for maintaining quality and efficacy of the...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-019-09889-7

    authors： Parhiz H,Ketcham SA,Zou G,Ghosh B,Fratz-Berilla EJ,Ashraf M,Ju T,Madhavarao CN

    更新日期：2019-08-01 00:00:00

  • Impacts of coexisting antibiotics, antibacterial residues, and heavy metals on the occurrence of erythromycin resistance genes in urban wastewater.

    abstract：:Antibiotic resistance is a global challenge and represents a growing threat on human health worldwide. Wastewater treatment plants (WWTPs) are generally considered as hotspots for control and/or dissemination of antibiotic resistance. The role of antibiotics, antibacterial residues, and heavy metals played on the evol...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-015-6404-9

    authors： Gao P,He S,Huang S,Li K,Liu Z,Xue G,Sun W

    更新日期：2015-05-01 00:00:00

  • SYBR®Green qPCR Salmonella detection system allowing discrimination at the genus, species and subspecies levels.

    abstract：:In this work, a three-level Salmonella detection system based on a combination of seven SYBR®Green qPCR was developed. This detection system discriminates Salmonella at the genus, species and subspecies levels using a single 96-well plate. The SYBR®Green qPCR assays target the invA, rpoD, iroB and safC genes, as well ...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-013-5234-x

    authors： Barbau-Piednoir E,Bertrand S,Mahillon J,Roosens NH,Botteldoorn N

    更新日期：2013-11-01 00:00:00

  • Boosting the efficiency of site-saturation mutagenesis for a difficult-to-randomize gene by a two-step PCR strategy.

    abstract：:Site-saturation mutagenesis (SSM) has been used in directed evolution of proteins for a long time. As a special form of saturation mutagenesis, it involves individual randomization at a given residue with formation of all 19 amino acids. To date, the most efficient embodiment of SSM is a one-step PCR-based approach us...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-018-9041-2

    authors： Li A,Acevedo-Rocha CG,Reetz MT

    更新日期：2018-07-01 00:00:00

  • Screening of a novel strong promoter by RNA sequencing and its application to H2 production in a hyperthermophilic archaeon.

    abstract：:A strong promoter increases transcription of the genes of interest and enhances the production of various valuable substances. For a hyperthermophilic archaeon Thermococcus onnurineus NA1, which can produce H2 from carbon monoxide oxidation, we searched for a novel endogenous strong promoter by transcriptome analysis ...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-015-6444-1

    authors： Lee SH,Kim MS,Jung HC,Lee J,Lee JH,Lee HS,Kang SG

    更新日期：2015-05-01 00:00:00

  • Identification of the phd gene cluster responsible for phenylpropanoid utilization in Corynebacterium glutamicum.

    abstract：:Phenylpropanoids as abundant, lignin-derived compounds represent sustainable feedstocks for biotechnological production processes. We found that the biotechnologically important soil bacterium Corynebacterium glutamicum is able to grow on phenylpropanoids such as p-coumaric acid, ferulic acid, caffeic acid, and 3-(4-h...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-015-7165-1

    authors： Kallscheuer N,Vogt M,Kappelmann J,Krumbach K,Noack S,Bott M,Marienhagen J

    更新日期：2016-02-01 00:00:00

  • Synthesis of biosurfactants in extreme conditions.

    abstract：:The interest in industrial biotechnology and its importance opens up challenging possibilities of research in this area. Surfactants have long been among the most versatile of process chemicals. Their market is extremely competitive and manufacturers will have to expand their arsenal to develop products for the year 2...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章,评审

    doi：10.1007/s002530051329

    authors： Cameotra SS,Makkar RS

    更新日期：1998-11-01 00:00:00

  • Initial stages of microbiologically influenced tarnishing on titanium after 20 months of immersion in freshwater.

    abstract：:This paper studies the initial stages of iridescent tarnishes on titanium heat exchanger tubes in contact with running freshwater on the river Tagus in Spain for up to 20 months. Electrochemical impedance spectroscopy (EIS), scanning electron microscopy [(SEM with energy dispersive X-ray (EDX)] and X-ray photoelectron...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-003-1472-7

    authors： Moreno DA,Cano E,Ibars JR,Polo JL,Montero F,Bastidas JM

    更新日期：2004-05-01 00:00:00

  • Use of lipases in the resolution of racemic ibuprofen.

    abstract：:Resolution of (R,S)-ibuprofen enantiomers by esterification in different organic solvents was studied using Candida cylindracea lipase. This enzyme preparation had high enantiospecificity for S(+)-ibuprofen in the esterification reaction of a racemic ibuprofen with primary alcohols. The esterification yields of second...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/BF00169420

    authors： Mustranta A

    更新日期：1992-10-01 00:00:00

  • Screening of high-yielding biocontrol bacterium Bs-916 mutant by ion implantation.

    abstract：:Bacillus subtilis 916 was an effective biocontrol agent in control rice sheath blight caused by Rhizoctonia solani. To further improve its antagonistic ability, low-energy ion implantation was applied in Bs-916. We studied the effects of different doses of N(+) implantation. The optimum dose of ion implantation for th...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-007-0951-7

    authors： Li D,Nie F,Wei L,Wei B,Chen Z

    更新日期：2007-07-01 00:00:00

  • Mercury alters the bacterial community structure and diversity in soil even at concentrations lower than the guideline values.

    abstract：:This study evaluated the effect of inorganic mercury (Hg) on bacterial community and diversity in different soils. Three soils-neutral, alkaline and acidic-were spiked with six different concentrations of Hg ranging from 0 to 200 mg kg-1 and aged for 90 days. At the end of the ageing period, 18 samples from three diff...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-016-7965-y

    authors： Mahbub KR,Subashchandrabose SR,Krishnan K,Naidu R,Megharaj M

    更新日期：2017-03-01 00:00:00

  • Vinyl ketone reduction by three distinct Gluconobacter oxydans 621H enzymes.

    abstract：:Three cytosolic NADPH-dependent flavin-associated proteins (Gox2107, Gox0502, and Gox2684) from Gluconobacter oxydans 621H were overproduced in Escherichia coli, and the recombinant enzymes were purified and characterized. Apparent native molecular masses of 65.2, 78.2, and 78.4 kDa were observed for Gox2107, Gox0502,...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-008-1600-5

    authors： Schweiger P,Gross H,Wesener S,Deppenmeier U

    更新日期：2008-10-01 00:00:00

  • Studies on the Pycnoporus sanguineus CCT-4518 laccase purified by hydrophobic interaction chromatography.

    abstract：:A laccase from Pycnoporus sanguineus was purified by two steps using phenyl-Sepharose columm. A typical procedure provided 54.1-fold purification, with a yield of 8.37%, using syringaldazine as substrate. The molecular weight of the purified laccase was 69 and 68 kDa as estimated by 12% (w/v) SDS-PAGE gel and by gel f...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-006-0817-4

    authors： Garcia TA,Santiago MF,Ulhoa CJ

    更新日期：2007-05-01 00:00:00

  • Biodiscovery from rare actinomycetes: an eco-taxonomical perspective.

    abstract：:Microbial natural products, in particular, the ones produced by the members of the order Actinomycetales, will continue to represent an important route to the discovery of novel classes of bioactive compounds. As a result, the search for and discovery of lesser-known and/or novel actinomycetes is of significant intere...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章,评审

    doi：10.1007/s00253-012-3898-2

    authors： Kurtböke DI

    更新日期：2012-03-01 00:00:00

  • Mutants blocked in penicillin biosynthesis show a deletion of the entire penicillin gene cluster at a specific site within a conserved hexanucleotide sequence.

    abstract：:The organization of the genes of the penicillin cluster has been studied in three different mutants of P. chrysogenum impaired in penicillin biosynthesis. The three blocked mutants (derived from the parental strain P. chrysogenum Bb-1) lacked the genes pcbAB, pcbC and penDE of the penicillin biosynthetic pathway and w...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/BF00172491

    authors： Fierro F,Montenegro E,Gutiérrez S,Martín JF

    更新日期：1996-01-01 00:00:00

  • Efficient induction of ginsenoside biosynthesis and alteration of ginsenoside heterogeneity in cell cultures of Panax notoginseng by using chemically synthesized 2-hydroxyethyl jasmonate.

    abstract：:Chemically synthesized 2-hydroxyethyl jasmonate (HEJA) was for the first time employed to induce the ginsenoside biosynthesis and to manipulate the product heterogeneity in plant cell cultures. The dose response and timing of HEJA elicitation were investigated in cell suspension cultures of Panax notoginseng. The opti...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-005-0089-4

    authors： Wang W,Zhao ZJ,Xu Y,Qian X,Zhong JJ

    更新日期：2006-04-01 00:00:00

  • Biosynthesis of isocitric acid in repeated-batch culture and testing of its stress-protective activity.

    abstract：:Biosynthesis of Ds(+)-threo-isocitric acid from ethanol in the Yarrowia lipolytica batch and repeated-batch cultures was studied. Repeated-batch cultivation was found to provide for a good biosynthetic efficiency of the producer for as long as 748 h, probably due to maintenance of high activities of enzymes involved i...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-019-09729-8

    authors： Morgunov IG,Kamzolova SV,Karpukhina OV,Bokieva SB,Inozemtsev AN

    更新日期：2019-04-01 00:00:00

  • Modelling and parameter identification for batch fermentations with Streptomyces tendae under phosphate limitation.

    abstract：:A simple structured model for the dynamics of phosphate-limited batch fermentations with Streptomyces tendae is presented. The model describes the influence of intracellular phosphate storage upon the growth behav our of the culture. The development of the model takes into account the possible internal regulatory proc...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/BF00172717

    authors： Mundry C,Kuhn KP

    更新日期：1991-06-01 00:00:00

  • Isolation of a novel high erythritol-producing Pseudozyma tsukubaensis and scale-up of erythritol fermentation to industrial level.

    abstract：:This study isolated a novel erythritol-producing yeast strain, which is capable of growth at high osmolarity. Characteristics of the strain include asexual reproduction by multilateral budding, absence of extracellular starch-like compounds, and a negative Diazonium blue B color reaction. Phylogenetic analysis based o...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-009-1871-5

    authors： Jeya M,Lee KM,Tiwari MK,Kim JS,Gunasekaran P,Kim SY,Kim IW,Lee JK

    更新日期：2009-05-01 00:00:00

  • Engineering Escherichia coli for efficient cellobiose utilization.

    abstract：:Escherichia coli normally cannot utilize the β-glucoside sugar cellobiose as a carbon and energy source unless a stringent selection pressure for survival is present. The cellobiose-utilization phenotype can be conferred by mutations in the two cryptic operons, chb and asc. In this study, the cellobiose-utilization ph...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-011-3434-9

    authors： Vinuselvi P,Lee SK

    更新日期：2011-10-01 00:00:00

  • A newly designed degenerate PCR primer based on pmoA gene for detection of nitrite-dependent anaerobic methane-oxidizing bacteria from different ecological niches.

    abstract：:A new pmoA gene-based PCR primer set was designed for detection of nitrite-dependent anaerobic oxidation of methane (n-damo) bacteria from four different ecosystems, namely rice paddy soil, freshwater reservoir, reed bed, and sludge from wastewater treatment plant. This primer set showed high specificity and efficienc...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-013-5260-8

    authors： Han P,Gu JD

    更新日期：2013-12-01 00:00:00

  • Physiological heterogeneity of Pseudomonas taetrolens during lactobionic acid production.

    abstract：:Physiological heterogeneity constitutes a critical parameter in biotechnological systems since both metabolite yield and productivity are often hampered by the presence of undesired physiological cell subpopulations. In the present study, the physiological status and functionality of Pseudomonas taetrolens cells were ...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-012-4254-2

    authors： Alonso S,Rendueles M,Díaz M

    更新日期：2012-12-01 00:00:00

  • Genetic characterisation and heterologous expression of leucocin C, a class IIa bacteriocin from Leuconostoc carnosum 4010.

    abstract：:Leuconostoc carnosum 4010 is a protective culture for meat products. It kills the foodborne pathogen Listeria monocytogenes by producing two class IIa (pediocin-like) bacteriocins, leucocin A and leucocin C. The genes for leucocin A production have previously been characterised from Leuconostoc gelidum UAL 187, wherea...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-012-4406-4

    authors： Wan X,Li R,Saris PE,Takala TM

    更新日期：2013-04-01 00:00:00

  • Analysis of genomic characters reveals that four distinct gene clusters are correlated with different functions in Burkholderia cenocepacia AU 1054.

    abstract：:Possessing three circular chromosomes is a distinct genomic characteristic of Burkholderia cenocepacia AU 1054, a clinically important pathogen in cystic fibrosis. In this study, base composition, codon usage and functional role category were analyzed in the B. cenocepacia AU 1054 genome. Although no bias in the base ...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-013-5415-7

    authors： Yuan J,Yang M,Ren J,Fu B,Jiang F,Zhang X

    更新日期：2014-01-01 00:00:00

  • Enhanced thermal stability of Pseudomonas aeruginosa lipoxygenase through modification of two highly flexible regions.

    abstract：:Lipoxygenase (LOX; EC 1.13.11.12) is an enzyme which is widely used in food industry to improve aroma and rheological or baking properties of foods. A series of studies have proven that the flexible regions negatively relates to the thermal stability of enzymes. In this study, two highly flexible regions, residues(20-...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-013-5039-y

    authors： Lu X,Liu S,Feng Y,Rao S,Zhou X,Wang M,Du G,Chen J

    更新日期：2014-02-01 00:00:00

  • Molecular cloning and expression of the pyranose 2-oxidase cDNA from Trametes ochracea MB49 in Escherichia coli.

    abstract：:A cDNA of a structural gene encoding pyranose 2-oxidase (P2O) from Trametes ochracea strain MB49 was cloned into Escherichia coli strain BL21(DE3) on a multicopy plasmid under the control of the trc promoter. Synthesis of P2O was studied in batch cultures in LB or M9-based mineral medium at 28 degrees C. While there w...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-003-1516-z

    authors： Vecerek B,Maresová H,Kocanová M,Kyslík P

    更新日期：2004-05-01 00:00:00

  • Metabolic pathway optimization using ribosome binding site variants and combinatorial gene assembly.

    abstract：:The genes encoding the mevalonate-based farnesyl pyrophosphate (FPP) biosynthetic pathway were encoded in two operons and expressed in Escherichia coli to increase the production of sesquiterpenes. Inefficient translation of several pathway genes created bottlenecks and led to the accumulation of several pathway inter...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-013-5361-4

    authors： Nowroozi FF,Baidoo EE,Ermakov S,Redding-Johanson AM,Batth TS,Petzold CJ,Keasling JD

    更新日期：2014-02-01 00:00:00