Abstract：
:A simple structured model for the dynamics of phosphate-limited batch fermentations with Streptomyces tendae is presented. The model describes the influence of intracellular phosphate storage upon the growth behav our of the culture. The development of the model takes into account the possible internal regulatory processes of phosphate metabolism. These complex biochemical pathways are summarized with regard to rate-limiting steps to obtain relatively simple model equations. The model parameters are fitted to the experimental data with an identification programme based on the sequential quadratic programming algorithm. Modifications in this algorithm yield a good performance for this application. With respect to the sensitivity of the model parameters, a feedback on the modelling is given. After several loops of modelling and identification, a model was achieved that fits to a set of batch fermentations. Furthermore the simulations show that RNA measurements of some recent fermentations can be interpreted by the simulated internal state variable and that there is evidence for RNA as an intracellular phosphate reserve.
journal_name
Appl Microbiol Biotechnoljournal_title
Applied microbiology and biotechnologyauthors
Mundry C,Kuhn KPdoi
10.1007/BF00172717subject
Has Abstractpub_date
1991-06-01 00:00:00pages
306-311issue
3eissn
0175-7598issn
1432-0614journal_volume
35pub_type
杂志文章abstract：:1,3-propanediol (1,3-PD) was produced with a robust fermentation process using waste glycerol feedstock from biodiesel production and a soil-based bacterial inoculum. An iterative inoculation method was developed to achieve independence from soil and selectively breed bacterial populations capable of glycerol metaboli...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-014-6259-5
更新日期：2015-03-01 00:00:00
abstract：:Gelatin zymography revealed the presence of proteolytic activity in conditioned medium (CM) from a serum-free, non-infected Spodoptera frugiperda, Sf9 insect cell culture. Two peptidase bands at about 49 and 39 kDa were detected and found to be proform and active form of the same enzyme. The 49-kDa form was visible on...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-005-0181-9
更新日期：2006-07-01 00:00:00
abstract：:Immobilization of the Geotrichum candidum (CCT 1205) cell with functionalized silica creates promising biocatalysts for production of ɛ-caprolactone. The results obtained by immobilization of the whole cell on SiO2-NH2 and SiO2-SH supports indicate that the presence of reactive functional groups on the support may pro...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-020-10875-7
更新日期：2020-10-01 00:00:00
abstract：:In this study, we investigated the possibility of using a modified hydantoinase process for the production of optically pure β-amino acids. Two aryl-substituted dihydropyrimidines D,L-6-phenyl-5,6-dihydrouracil (PheDU) and para-chloro-D,L-6-phenyl-5,6-dihydrouracil (pClPheDU) were synthesized. Hydrolysis of these nove...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-011-3691-7
更新日期：2012-06-01 00:00:00
abstract：:Aspergillus oryzae is a fungus used extensively in the fermentation industry. We constructed cDNA microarrays comprising 2,070 highly expressed cDNAs selected from the approximately 6,000 non-redundant expressed sequence tags (ESTs) in the A. oryzae EST database (http://www.aist.go.jp/RIODB/ffdb/index.html). Using the...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-004-1608-4
更新日期：2004-07-01 00:00:00
abstract：:Despite airborne microorganisms representing a relevant fraction of atmospheric suspended particles, only a small amount of information is currently available on their abundance and diversity and very few studies have investigated the environmental factors influencing the structure of airborne bacterial communities. I...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-012-4450-0
更新日期：2013-07-01 00:00:00
abstract：:The recent, rapid increase in bacterial antimicrobial resistance has become a major public health concern. One approach to generate new classes of antibacterials is targeting virulence rather than the viability of bacteria. Proteins of the Dsb system, which play a key role in the virulence of many pathogenic microorga...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s00253-017-8291-8
更新日期：2017-05-01 00:00:00
abstract：:In microorganisms, the enzyme acetate kinase (AK) catalyses the formation of ATP from ADP by de-phosphorylation of acetyl phosphate into acetic acid. A mutant strain of Clostridium acetobutylicum lacking acetate kinase activity is expected to have reduced acetate and acetone production compared to the wild type. In th...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-011-3848-4
更新日期：2012-05-01 00:00:00
abstract：:Grapevines are sensitive to a wide range of fungal pathogens, including agents such as Phaeomoniella chlamydospora and Phaeoacremonium aleophilum that cause tracheomycosis. In the present study, a procedure for DNA extraction from grapevine woody tissue is first evaluated and shown to be suitable for quantitative anal...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-013-5299-6
更新日期：2013-12-01 00:00:00
abstract：:Clostridium acetobutylicum is an important industrial microorganism for n-butanol bioproduction, and its transcription factor AbrB0310 regulates various important cellular processes. However, the roles of two abrB homologues, abrB1941 and abrB3647, have not been determined because they appear inactive during transcrip...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-016-7638-x
更新日期：2016-11-01 00:00:00
abstract：:Wastewater treatment plants (WWTPs) are major collection pools of antibiotics of which low concentrations may induce antibiotic resistance in their microbial communities and pose threat to human health. However, information is still limited on the microbial community alteration in WWTPs upon exposure to low-dose antib...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-013-5205-2
更新日期：2014-03-01 00:00:00
abstract：:A new strain, Enterobacter sp. ECU1107, was identified among over 200 soil isolates using a two-step screening strategy for the enantioselective synthesis of (2S,3R)-3-phenylglycidate methyl ester (PGM), a key intermediate for production of a potent anticancer drug Taxol®. An organic-aqueous biphasic system was employ...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-012-4435-z
更新日期：2013-07-01 00:00:00
abstract：:Nitrite-dependent anaerobic methane oxidation (N-DAMO), which couples anaerobic methane oxidation and nitrite reduction, is a recently discovered bioprocess coupling microbial nitrogen and carbon cycles. The discovery of this microbial process challenges the traditional knowledge of global methane sinks and nitrogen l...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-017-8521-0
更新日期：2017-11-01 00:00:00
abstract：:A soluble glucoside 3-dehydrogenase (G3DH) from Stenotrophomonas maltrophilia CCTCC M 204024, recently isolated from wheat soil in our laboratory, was purified to 37.4-fold with a yield of 24.7% and was estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis with a molecular mass of 66 kDa. 2,6-Dichloro...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-005-0201-9
更新日期：2006-08-01 00:00:00
abstract：:A laccase from Pycnoporus sanguineus was purified by two steps using phenyl-Sepharose columm. A typical procedure provided 54.1-fold purification, with a yield of 8.37%, using syringaldazine as substrate. The molecular weight of the purified laccase was 69 and 68 kDa as estimated by 12% (w/v) SDS-PAGE gel and by gel f...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-006-0817-4
更新日期：2007-05-01 00:00:00
abstract：:Anode properties are critical for the performance of microbial electrolysis cells (MECs). In the present study, Fe nanoparticle-modified graphite disks were used as anodes to investigate the effects of nanoparticles on the performance of Shewanella oneidensis MR-1 in MECs. Results demonstrated that the average current...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-011-3643-2
更新日期：2012-01-01 00:00:00
abstract：:A drawback of biotechnological processes, where microorganisms convert biomass constituents, such as starch, cellulose, hemicelluloses, lipids, and proteins, into wanted products, is the economic feasibility. Particularly the cost of nitrogen sources in biotechnological processes can make up a large fraction of total ...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s00253-015-7278-6
更新日期：2016-03-01 00:00:00
abstract：:A Gram-negative anaerobic microorganism, MRG-1, isolated from human intestine showed high activities of deglycosylation and reduction of daidzin, based on rapid TLC analysis. A rod-shaped strain MRG-1 was identified as a new species showing 91.0% homology to Coprobacillus species, based on 16S rRNA sequence analysis. ...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-011-3310-7
更新日期：2011-08-01 00:00:00
abstract：:In Catharanthus roseus cell cultures the time courses of four enzyme activities, tryptophan decarboxylase (TDC), strictosidine synthase (SSS), geraniol-10-hydroxylase (G10H) and anthranilate synthase (AS), and alkaloid accumulation were compared under two different culture conditions (low-inoculum density and high-ino...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/BF00166846
更新日期：1993-04-01 00:00:00
abstract：:Sustainable tea cultivation will rely increasingly on alternatives to conventional chemical insecticides for pest management that are environment-friendly and reduce the amount of pesticide residues in made tea. Entomopathogens can provide effective control, conserve biodiversity, and serve as alternatives to chemical...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s00253-014-5749-9
更新日期：2014-06-01 00:00:00
abstract：:Atherosclerosis is a major cause of mortalities and morbidities worldwide. It is associated with hyperlipidemia and inflammation, and become chronic by triggering metabolites in different metabolic pathways. Disturbance in the human gut microbiota is now considered a critical factor in the atherosclerosis. Trimethylam...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s00253-019-10142-4
更新日期：2019-12-01 00:00:00
abstract：:The application of biocontrol biopesticides based on plant growth-promoting rhizobacteria (PGPR), particularly members of the genus Bacillus, is considered a promising perspective to make agricultural practices sustainable and ecologically safe. Recent advances in genome sequencing by third-generation sequencing techn...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-020-10767-w
更新日期：2020-09-01 00:00:00
abstract：:The utilization of monochlorobenzoate isomers (2-, 3- and 4-chlorobenzoate) by anaerobic microbial consortia in River Nile sediments was systematically evaluated under denitrifying, Fe-reducing, sulfidogenic and methanogenic conditions. Loss of all three chlorobenzoates was noted in denitrifying cultures; furthermore,...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/BF02431930
更新日期：1995-10-01 00:00:00
abstract：:Vanillin can be produced on a commercial scale by depolymerising renewable lignin. One product of microbial metabolism of vanillin by common soil microbes, such as Acinetobacter baylyi, is a tricarboxylic acid with a butadiene backbone known as 3-carboxy muconate (3CM). Three enzymes, 4-hydroxy benzaldehyde dehydrogen...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-010-3078-1
更新日期：2011-04-01 00:00:00
abstract：:There is an error in the Original Publication of this paper for "Acknowledgements" section was missing. ...
journal_title：Applied microbiology and biotechnology
pub_type： 已发布勘误
doi：10.1007/s00253-020-10424-2
更新日期：2020-03-01 00:00:00
abstract：:Sucrose is the most abundant disaccharide in the environment because of its origin in higher plant tissues, and many Eubacteria possess catalytic enzymes, such as the sucrose-6-phosphate hydrolases and sucrose phosphorylases, that enable them to metabolise this carbohydrate in a regulated manner. This review describes...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s00253-004-1885-y
更新日期：2005-05-01 00:00:00
abstract：:DNA microarrays have found widespread use as a flexible tool to investigate bacterial metabolism. Their main advantage is the comprehensive data they produce on the transcriptional response of the whole genome to an environmental or genetic stimulus. This allows the microbiologist to monitor metabolism and to define s...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s00253-006-0584-2
更新日期：2006-11-01 00:00:00
abstract：:To seek more information on function of two-component regulatory systems (TCSs) in Streptomyces coelicolor, a dozen TCS-knockout mutants were generated, and phenotype changes were determined. One TCS (SCO5403/5404)-deleted mutant with phenotype change was obtained. Here, we report the characterization of this novel TC...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-007-1184-5
更新日期：2007-12-01 00:00:00
abstract：:(R)-[3,5-bis(trifluoromethyl)phenyl] ethanol ((R)-BTPE) is a valuable chiral intermediate for the synthesis of antiemetic drug Aprepitant and Fosaprepitant. A Leifsonia xyli HS0904-derived carbonyl reductase (LXCAR), an effective biocatalyst for the asymmetric reduction of 3,5-bis(trifluoromethyl) acetophenone (BTAP) ...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-014-5770-z
更新日期：2014-10-01 00:00:00
abstract：:Streptomyces coelicolor A3(2) is a rubber-degrading actinomycete that harbors one gene coding for a latex clearing protein (lcpA3(2)). Within the genome of S. coelicolor A3(2), we identified a gene coding for a novel protein of the TetR family (LcpRBA3(2)) downstream of lcpA3(2) and demonstrated its binding upstream o...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-019-09896-8
更新日期：2019-07-01 00:00:00
