Bacterial thiol oxidoreductases - from basic research to new antibacterial strategies.

abstract：:Natural attenuation maybe a cost-efficient option for bioremediation of contaminated sites but requires knowledge about the activity of degrading microbes under in situ conditions. In order to link microbial activity to the spatial distribution of contaminant degraders, we combined the recently improved in situ microc...

journal_title：Applied microbiology and biotechnology

authors： Schurig C,Mueller CW,Höschen C,Prager A,Kothe E,Beck H,Miltner A,Kästner M

更新日期：2015-01-01 00:00:00

abstract：:We have characterized a mutant of Streptomyces clavuligerus NRRL 3585 which is almost completely blocked in cephalosporin biosynthesis and exhibits depressed activities of both the delta(L-alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV) synthetase and cyclase enzymes of the cephalosporin pathway. A wild-type DNA region ...

journal_title：Applied microbiology and biotechnology

authors： Piret J,Resendiz B,Mahro B,Zhang JY,Serpe E,Romero J,Connors N,Demain AL

更新日期：1990-02-01 00:00:00

abstract：:Bacillus pumilus mutants were generated by targeted deletion of a set of genes eventually facilitating genetic handling and assuring biological containment. The well-defined and stable mutants do not form functional endospores due to the deletion of yqfD, an essential sporulation gene; they are affected in DNA repair,...

journal_title：Applied microbiology and biotechnology

authors： Wemhoff S,Meinhardt F

更新日期：2013-09-01 00:00:00

abstract：:To develop a high efficiency Candida antarctica lipase B (CALB) yeast display system, we linked two CALB genes fused with Sacchromyces cerevisiae cell wall protein genes, the Sed1 and the 3'-terminal half of Sag1, separately by a 2A peptide of foot-and-mouth disease virus (FMDV) in a single open reading frame. The CAL...

journal_title：Applied microbiology and biotechnology

authors： Sun YF,Lin Y,Zhang JH,Zheng SP,Ye YR,Liang XX,Han SY

更新日期：2012-12-01 00:00:00

abstract：:Grape juice contains about equal amounts of glucose and fructose, but wine strains of Saccharomyces cerevisiae ferment glucose slightly faster than fructose, leading to fructose concentrations that exceed glucose concentrations in the fermenting must. A high fructose/glucose ratio may contribute to sluggish and stuck ...

journal_title：Applied microbiology and biotechnology

authors： Berthels NJ,Cordero Otero RR,Bauer FF,Pretorius IS,Thevelein JM

更新日期：2008-01-01 00:00:00

abstract：:An efficient fedbatch process for the production of Lactobacillus kefir DSM 20587 cells was developed. An improvement in space time yield of 270% (3.7 g(DCW) l(-1)-day(-1)) and in final enzyme activity of 440% (9.1 U/ml) was achieved on a 150 l scale by controlling the oxygen transfer rate to 7--9 mmol l(-1) h(-1). Th...

journal_title：Applied microbiology and biotechnology

authors： Pfruender H,Amidjojo M,Hang F,Weuster-Botz D

更新日期：2005-06-01 00:00:00

abstract：:The aim of this study was to develop a low-cost and environmental friendly treatment method for the removal of cationic dye from wastewater. Rice straw powder combined with the white rot fungus Phanerochaete chrysosporium (P. chrysosporium) were used to remove methylene blue (MB) from wastewater at varied operational ...

journal_title：Applied microbiology and biotechnology

authors： Cheng M,Zeng G,Huang D,Lai C,Wei Z,Li N,Xu P,Zhang C,Zhu Y,He X

更新日期：2015-06-01 00:00:00

abstract：:Inactivation of D-amino acid oxidase occurred by different mechanisms. The enzyme showed a rapid loss of activity in the presence of micromolar amounts of Cu2+ and Hg2+. It was also sensitive to oxidative inactivation by Fe2+ and H2O2 when both reagents were added in millimolar amounts. When oxidatively inactivated D-...

journal_title：Applied microbiology and biotechnology

authors： Schräder T,Andreesen JR

更新日期：1996-05-01 00:00:00

abstract：:A mixed methane-oxidizing biofilm was characterized, concurrently using a number of advanced techniques. Community analysis results by microarray exhibited that type II members dominated the methanotrophic community, in which Methylocystis was most abundant, followed by Methylosinus. Observation results by fluorescent...

journal_title：Applied microbiology and biotechnology

authors： Kim TG,Yi T,Lee EH,Ryu HW,Cho KS

更新日期：2012-08-01 00:00:00

abstract：:A characteristic mucosal immune response involves the production of antigen-specific secretory immunoglobulin A (SIgA) antibodies. In order to study transcytosis by mimicking the SIgA secretion and to screen for SIgA secretion-promoting substances, we developed a model system of a transfected Madin-Darby canine kidney...

journal_title：Applied microbiology and biotechnology

authors： Zuo T,Feng X,Zhang N,Xue C,Tang QJ

更新日期：2015-07-01 00:00:00

abstract：:Human epidermal growth factor receptors (EGFR) are an important target in drug discovery in terms of both protein-small-molecule interactions and protein-protein interactions. In this work, the isolation of a stable soluble protein of the tyrosine kinase domain of EGFR in Escherichia coli expression has been accomplis...

journal_title：Applied microbiology and biotechnology

authors： Seetaha S,Ratanabanyong S,Choowongkomon K

更新日期：2019-10-01 00:00:00

abstract：:Aspartic proteases are a relatively small group of proteolytic enzymes that are active in acidic environments and are found across all forms of life. Certain microorganisms secrete such proteases as virulence agents and/or in order to break down proteins thereby liberating assimilable sources of nitrogen. Some of the ...

journal_title：Applied microbiology and biotechnology

authors： Theron LW,Divol B

更新日期：2014-11-01 00:00:00

abstract：:Homologous recombination-based recombineering is a widely used DNA cloning and modification technique; recombineering efficiency improvement would be helpful for high-throughput DNA manipulation. Escherichia coli primase DnaG variants, such as DnaG Q576A and DnaG K580A, increase the recombineering efficiency via impai...

journal_title：Applied microbiology and biotechnology

authors： Li J,Sun J,Gao X,Wu Z,Shang G

更新日期：2019-04-01 00:00:00

abstract：:The dibenzothiophene (DBT) desulfurizing bacterium metabolizes DBT to form 2-hydroxybiphenyl without breaking the carbon skeleton. Of the DBT desulfurization enzymes, DszC and DszA catalyze monooxygenation reactions, both requiring flavin reductase. We searched for non-DBT-desulfurizing microorganisms producing a flav...

journal_title：Applied microbiology and biotechnology

authors： Ohshiro T,Aoi Y,Torii K,Izumi Y

更新日期：2002-09-01 00:00:00

abstract：:Whey, the main by-product of the cheese industry, is considered as an important pollutant due to its high chemical and biological oxygen demand. Whey, often considered as waste, has high nutritional value and can be used to obtain value-added products, although some of them need expensive enzymatic synthesis. An econo...

journal_title：Applied microbiology and biotechnology

authors： Pescuma M,de Valdez GF,Mozzi F

更新日期：2015-08-01 00:00:00

abstract：:The addition of humic acid (HA) to polycyclic aromatic hydrocarbon (PAH) contaminated systems has been shown to enhance, inhibit, or have no effect on the biodegradation of these PAHs. In this study, the surfactant effects of Elliott soil HA (ESHA) at two pH values were tested. At pH 7.0, ESHA did not behave as a surf...

journal_title：Applied microbiology and biotechnology

authors： Liang Y,Britt DW,McLean JE,Sorensen DL,Sims RC

更新日期：2007-04-01 00:00:00

abstract：:To understand the effect of daily bioaugmentation in full-scale grease interceptors (GIs), we compared the microbial communities occurring in two full-scale GIs during bioaugmented and non-bioaugmented cycles. The changes in microbial communities were determined using terminal restriction fragment length polymorphism ...

journal_title：Applied microbiology and biotechnology

authors： He X,So MJ,de Los Reyes FL 3rd

更新日期：2016-08-01 00:00:00

abstract：:Thermochemical processing of biomass by fast pyrolysis provides a nonenzymatic route for depolymerization of biomass into sugars that can be used for the biological production of fuels and chemicals. Fermentative utilization of this bio-oil faces two formidable challenges. First is the fact that most bio-oil-associate...

journal_title：Applied microbiology and biotechnology

authors： Jarboe LR,Wen Z,Choi D,Brown RC

更新日期：2011-09-01 00:00:00

abstract：:The activities of seven hydrolytic enzymes (L-alanine aminopeptidase, esterase, α-and β-glucosidase, phosphomonoesterase, phosphodiesterase, sulfatase) were monitored during 1 year in parallel and serial treatment units of the biological stage of a communal wastewater treatment plant. The spatial homogeneity of enzyme...

journal_title：Applied microbiology and biotechnology

authors： Kreutz JA,Böckenhüser I,Wacht M,Fischer K

更新日期：2016-08-01 00:00:00

abstract：:Rhodovulum sulfidophilum DSM-1374 is a potential producer of polyester when growing in phototrophic conditions. The present study investigated on a polyester product (P3HB) by culturing Rhodovulum sulfidophilum DSM-1374 in two different photobioreactors (PBR-1 and PBR-2) both with 4-L working volumes. PBR-1 is equippe...

journal_title：Applied microbiology and biotechnology

authors： Carlozzi P,Di Lorenzo T,Ghanotakis DF,Touloupakis E

更新日期：2020-03-01 00:00:00

abstract：:We report on a pheromone-based inter-species communication system, allowing for a controlled cell-cell communication between the two species Saccharomyces cerevisiae and Schizosaccharomyces pombe as a proof of principle. It exploits the mating response pathways of the two yeast species employing the pheromones, α- or ...

journal_title：Applied microbiology and biotechnology

authors： Hennig S,Clemens A,Rödel G,Ostermann K

更新日期：2015-02-01 00:00:00

abstract：:Bacterial glucans have aroused increasing interest in commercial applications in the food and pharmaceutical sectors. A number of bacterial glucans have been reported over recent decades, and their structure, production, and functional properties have been extensively studied. In this paper, we review recent researche...

journal_title：Applied microbiology and biotechnology

authors： Xu L,Zhang J

更新日期：2016-11-01 00:00:00

abstract：:Thiabendazole (TBZ) is a fungicide used in fruit-packaging plants. Its application leads to the production of wastewaters requiring detoxification. In the absence of efficient treatment methods, biological depuration of these effluents could be a viable alternative. However, nothing is known regarding the microbial de...

journal_title：Applied microbiology and biotechnology

authors： Perruchon C,Chatzinotas A,Omirou M,Vasileiadis S,Menkissoglou-Spiroudi U,Karpouzas DG

更新日期：2017-05-01 00:00:00

abstract：:Conventional acetone-butanol-ethanol (ABE) fermentation coupled with gas stripping is conducted under strict anaerobic conditions. In this work, a fed-batch ABE fermentation integrated with gas stripping (FAFIGS) system using a non-strict anaerobic butanol-producing symbiotic system, TSH06, was investigated for the ef...

journal_title：Applied microbiology and biotechnology

authors： Lin Z,Liu H,Yan X,Zhou Y,Cheng K,Zhang J

更新日期：2017-11-01 00:00:00

abstract：:Medicinal plants of the Dendrobium genus are highly prized, and hence, methodologies have been developed to authenticate Dendrobium drugs from its adulterants. Many bioactive constituents of Dendrobium species have been identified. The macromolecules included lectins; the enzymes chalcone synthase, sucrose synthase, a...

journal_title：Applied microbiology and biotechnology

authors： Ng TB,Liu J,Wong JH,Ye X,Wing Sze SC,Tong Y,Zhang KY

更新日期：2012-03-01 00:00:00

abstract：:In order to understand the role of N1 domain (1-257 aa) in the amylopullulanase (gt-apu) of the extremely thermophilic bacterium Geobacillus thermoleovorans NP33, N1 deletion construct (gt-apuΔN) has been generated and expressed in Escherichia coli. The truncated amylopullulanase (gt-apuΔN) exhibits similar pH and tem...

journal_title：Applied microbiology and biotechnology

authors： Nisha M,Satyanarayana T

更新日期：2015-07-01 00:00:00

abstract：:The formation and activity of an As(III)-oxidising biofilm in a bioreactor, using pozzolana as bacterial growth support, was studied for the purpose of optimising fixed-bed bioreactors for bioremediation. After 60 days of continuous functioning with an As(III)-contaminated effluent, the active biofilm was found to be ...

journal_title：Applied microbiology and biotechnology

authors： Michel C,Jean M,Coulon S,Dictor MC,Delorme F,Morin D,Garrido F

更新日期：2007-11-01 00:00:00

abstract：:Although acetate formation and tolerance are important criteria for various aspects of biotechnological process development, available studies on acetate tolerance in different species are disparate. We evaluate the response of eight bacterial strains, including two variants of Escherichia coli, two variants of Staphy...

journal_title：Applied microbiology and biotechnology

authors： Lasko DR,Zamboni N,Sauer U

更新日期：2000-08-01 00:00:00

abstract：:Recombinant production of mammalian cytoplasmic proteins plays a major role in developing pharmaceutical products. Here we describe two expression technologies using unique nature of halophilic bacteria. One of such properties of halophilic bacteria is accumulation of compatible solutes in the cytoplasm. As the compat...

journal_title：Applied microbiology and biotechnology

authors： Tokunaga H,Arakawa T,Tokunaga M

更新日期：2010-12-01 00:00:00

abstract：:Aromatic amino acid metabolism in yeast is an important source of secondary compounds that influence the aroma and flavour of alcoholic beverages and foods. Examples are the higher alcohol 2-phenylethanol, and its acetate ester, 2-phenylethyl acetate, which impart desirable floral aromas in wine, beer and baker's prod...

journal_title：Applied microbiology and biotechnology

authors： Cordente AG,Schmidt S,Beltran G,Torija MJ,Curtin CD

更新日期：2019-06-01 00:00:00