当前位置： SCI文献检索 > APPLIED MICROBIOLOGY AND BIOTECHNOLOGY期刊下所有文献 > Induction of ajmalicine formation and related enzyme activities in Catharanthus roseus cells: effect of inoculum density.

Induction of ajmalicine formation and related enzyme activities in Catharanthus roseus cells: effect of inoculum density.

Abstract：

:In Catharanthus roseus cell cultures the time courses of four enzyme activities, tryptophan decarboxylase (TDC), strictosidine synthase (SSS), geraniol-10-hydroxylase (G10H) and anthranilate synthase (AS), and alkaloid accumulation were compared under two different culture conditions (low-inoculum density and high-inoculum density on induction medium) and a control on growth medium. In growth medium a transient increase in TDC activity was first observed after which G10H reached its maximum activity; only tryptamine accumulated, no ajmalicine could be detected. Apparently, a concerted induction of enzyme activities is required for ajmalicine formation. Cells inoculated in induction medium showed such a concerted induction of AS, TDC and G10H activities. After 30 days the low-density culture had accumulated six times more ajmalicine (in mumoles/g) than the high-density culture. Thus, increase in biomass concentration (high-density cultures) did not enhance the total alkaloid production. The major differences observed in enzyme levels between high- and low-density cultures were in the AS and TDC activities, which were two to three times higher in the low-density culture, indicating that there is a positive correlation between ajmalicine formation and AS and TDC activities.

journal_name

Appl Microbiol Biotechnol

journal_title

Applied microbiology and biotechnology

authors

Moreno PR,Schlatmann JE,van der Heijden R,van Gulik WM,ten Hoopen HJ,Verpoorte R,Heijnen JJ

doi

10.1007/BF00166846

subject

Has Abstract

pub_date

1993-04-01 00:00:00

pages

42-7

issue

1

eissn

0175-7598

issn

1432-0614

journal_volume

39

pub_type

杂志文章

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