Comparison of engineered Saccharomyces cerevisiae and engineered Escherichia coli for the production of an optically pure keto alcohol.

abstract：:Rapid sugar consumption is important for the microbial production of chemicals and fuels. Here, we show that overexpression of the NADH dehydrogenase gene (ndh) increased glucose consumption rate in Corynebacterium glutamicum under oxygen-deprived conditions through investigating the relationship between the glucose c...

journal_title：Applied microbiology and biotechnology

authors： Tsuge Y,Uematsu K,Yamamoto S,Suda M,Yukawa H,Inui M

更新日期：2015-07-01 00:00:00

abstract：:To optimize the expression of cry genes in a Bacillus thuringiensis sigK mutant failing in crystal releasing, the transcriptional activity of the cry promoters cry1A, cry3A, cry4A, and cry8E was compared using lacZ gene fusions. A beta-galactosidase assay indicated that the cry8E promoter showed the highest transcript...

journal_title：Applied microbiology and biotechnology

authors： Zhou C,Zheng Q,Peng Q,Du L,Shu C,Zhang J,Song F

更新日期：2014-09-01 00:00:00

abstract：:The synthesis of rhamnosylated compounds has gained great importance since these compounds have potential therapeutic applications. The enzymatic approaches for glycosylation of bioactive molecules have been well developed; however, the enzymatic rhamnosylation has been largely hindered by lacking of the glycosyl dono...

journal_title：Applied microbiology and biotechnology

authors： Xu L,Liu X,Li Y,Yin Z,Jin L,Lu L,Qu J,Xiao M

更新日期：2019-10-01 00:00:00

abstract：:Oleaginous yeasts (18 strains) were grown in ethanol media under various cultivation conditions (33 biomass samples). It was found that lipid and lipid-free fractions of dry biomass have elemental composition and biomass reductivity very close to values which can be considered as biological constants. The energy conte...

journal_title：Applied microbiology and biotechnology

authors： Minkevich IG,Dedyukhina EG,Chistyakova TI

更新日期：2010-10-01 00:00:00

abstract：:Oxygenates, mainly methyl tert-butyl ether (MTBE), are commonly added to gasoline to enhance octane index and improve combustion efficiency. Other oxygenates used as gasoline additives are ethers such as ethyl tert-butyl ether (ETBE), tert-amyl methyl ether (TAME), and alcohols such as tert-butyl alcohol (TBA). As a r...

journal_title：Applied microbiology and biotechnology

authors： Fayolle F,Vandecasteele JP,Monot F

更新日期：2001-08-01 00:00:00

abstract：:Pullulan is a linear glucosic polysaccharide produced by the polymorphic fungus Aureobasidium pullulans, which has long been applied for various applications from food additives to environmental remediation agents. This review article presents an overview of pullulan's chemistry, biosynthesis, applications, state-of-t...

journal_title：Applied microbiology and biotechnology

authors： Cheng KC,Demirci A,Catchmark JM

更新日期：2011-10-01 00:00:00

abstract：:Expression of recombinant proteins in Escherichia coli often requires use of inducible promoters to shorten the lag phase and improve protein productivity and final protein titer. Synthetic molecules that cannot be metabolized by E. coli, such as isopropyl thiogalactopyranoside (IPTG), have been frequently used to tri...

journal_title：Applied microbiology and biotechnology

authors： Anilionyte O,Liang H,Ma X,Yang L,Zhou K

更新日期：2018-08-01 00:00:00

abstract：:A bacterial biofilm, capable of mineralising a technical mixture of cis- and trans-1,3-dichloropropene (DCPE), was enriched on the biomedium side of an extractive membrane biofilm reactor (EMBR). The membrane separates the biomedium from the industrial waste water, in terms of pH, ionic strength and the concentration ...

journal_title：Applied microbiology and biotechnology

authors： Katsivela E,Bonse D,Krüger A,Strömpl C,Livingston A,Wittich RM

更新日期：1999-11-01 00:00:00

abstract：:Enzymes are natural catalysts highly specific to the substrate type and operate under mild conditions of temperature, pressure, and pH with high conversion rates, which makes them more efficient than conventional chemical catalysts. The enzymes can be obtained from various sources, animal, vegetable, and microbiologic...

journal_title：Applied microbiology and biotechnology

authors： Filho DG,Silva AG,Guidini CZ

更新日期：2019-09-01 00:00:00

abstract：:A novel beta-glucosidase (BGL)-producing strain was isolated and identified as Penicillium purpurogenum KJS506 based on its morphology and internal transcribed spacer (ITS) rDNA gene sequence. When rice straw and corn steep powder were used as carbon and nitrogen sources, respectively, the maximal BGL activity of 12.3...

journal_title：Applied microbiology and biotechnology

authors： Jeya M,Joo AR,Lee KM,Tiwari MK,Lee KM,Kim SH,Lee JK

更新日期：2010-05-01 00:00:00

abstract：:The nitrile hydratase (NHase, EC 4.2.1.84) genes (alpha and beta subunit) and the corresponding activator gene from Rhodococcus equi TG328-2 were cloned and sequenced. This Fe-type NHase consists of 209 amino acids (alpha subunit, M(r) 23 kDa) and 218 amino acids (beta subunit, M(r) 24 kDa) and the NHase activator of ...

journal_title：Applied microbiology and biotechnology

authors： Rzeznicka K,Schätzle S,Böttcher D,Klein J,Bornscheuer UT

更新日期：2010-02-01 00:00:00

abstract：:Potato pulp is a poorly utilized, high-volume co-processing product resulting from industrial potato starch manufacturing. Potato pulp mainly consists of the tuber plant cell wall material and is particularly rich in pectin, notably galactan branched rhamnogalacturonan I type pectin which has previously been shown to ...

journal_title：Applied microbiology and biotechnology

authors： Thomassen LV,Vigsnæs LK,Licht TR,Mikkelsen JD,Meyer AS

更新日期：2011-05-01 00:00:00

abstract：:Although yeasts are known to be able to incorporate a wide variety of exogenous sterols under strict anaerobiosis, no data are available on the assimilation of grapevine phytosterols under enological conditions and the eventual impact on fermentation kinetics. We used therefore a mixture of pure phytosterols, in a pro...

journal_title：Applied microbiology and biotechnology

authors： Luparia V,Soubeyrand V,Berges T,Julien A,Salmon JM

更新日期：2004-07-01 00:00:00

abstract：:α-Galacto-oligosaccharides (α-GOS) are produced by transgalactosylation reactions of α-galactosidase (α-Gal) or by conversion of raffinose family oligosaccharides by levansucrase. Similarly to β-GOS, α-GOS have the potential to mimic glycan receptors on eukaryotic cells and act as molecular decoys to prevent bacterial...

journal_title：Applied microbiology and biotechnology

authors： Wang Y,Black BA,Curtis JM,Gänzle MG

更新日期：2014-03-01 00:00:00

abstract：:The sustainable and practical degradation of intact chicken feathers by a newly isolated thermophilic bacterium Meiothermus ruber H328 was presented with extensive data. Aerobic cultivation with moderately thermophilic strain H328 at 55 degrees C for 6 days led to the apparently complete decay of the truly intact feat...

journal_title：Applied microbiology and biotechnology

authors： Matsui T,Yamada Y,Mitsuya H,Shigeri Y,Yoshida Y,Saito Y,Matsui H,Watanabe K

更新日期：2009-04-01 00:00:00

abstract：:The basidiomycetous yeast genus Pseudozyma produce large amounts of mannosylerythritol lipids (MELs), which are biosurfactants. A few Pseudozyma strains produce mono-acylated MEL as a minor compound using excess glucose as the sole carbon source. Mono-acylated MEL shows higher hydrophilicity than di-acylated MEL and h...

journal_title：Applied microbiology and biotechnology

authors： Saika A,Utashima Y,Koike H,Yamamoto S,Kishimoto T,Fukuoka T,Morita T

更新日期：2018-02-01 00:00:00

abstract：:Antimicrobial peptides/proteins (AMPs) are biologically active molecules with diverse structural properties that are produced by mammals, plants, insects, ticks, and microorganisms. They have a range of antibacterial, antifungal, antiviral, and even anticancer activities, and their biological properties could therefor...

journal_title：Applied microbiology and biotechnology

authors： Tonk M,Vilcinskas A,Rahnamaeian M

更新日期：2016-09-01 00:00:00

abstract：:Exposure to bioaerosols can adversely influence human health through respiratory tract, eye, and skin irritation. Bioaerosol composition is unique on the International Space Station (ISS), where the size distribution of particles in the air differs from those on Earth. This is due to the lack of gravitational settling...

journal_title：Applied microbiology and biotechnology

authors： Haines SR,Bope A,Horack JM,Meyer ME,Dannemiller KC

更新日期：2019-09-01 00:00:00

abstract：:Extracellular manganese peroxidase (MnP) was purified from the compost extract of Agaricus bisporus using anion exchange chromatography, gel filtration and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Two forms (MnP1 and MnP2) were separated by isoelectric focusing and their isoelectric points...

journal_title：Applied microbiology and biotechnology

authors： Lankinen VP,Bonnen AM,Anton LH,Wood DA,Kalkkinen N,Hatakka A,Thurston CF

更新日期：2001-03-01 00:00:00

abstract：:A simple structured model for the dynamics of phosphate-limited batch fermentations with Streptomyces tendae is presented. The model describes the influence of intracellular phosphate storage upon the growth behav our of the culture. The development of the model takes into account the possible internal regulatory proc...

journal_title：Applied microbiology and biotechnology

authors： Mundry C,Kuhn KP

更新日期：1991-06-01 00:00:00

abstract：:Influence of pressure (P) and oxygen partial pressure (PO2) on cultivation of various Streptomyces spp. and Micromonospora purpurea was examined in pressurized air-lift and stirred tank fermenters. The maximum PO2 was 2100 mbar. Growth and product formation of all cultures tested were markedly influenced by PO2 higher...

journal_title：Applied microbiology and biotechnology

authors： Liefke E,Kaiser D,Onken U

更新日期：1990-03-01 00:00:00

abstract：:Microalgal based biofuels are discussed as future sustainable energy source because of their higher photosynthetic and water use efficiency to produce biomass. In the context of climate CO2 mitigation strategies, algal mass production is discussed as a potential CO2 sequestration technology which uses CO2 emissions to...

journal_title：Applied microbiology and biotechnology

authors： Wilhelm C,Jakob T

更新日期：2011-12-01 00:00:00

abstract：:Isoprenoids, also referred to as terpenes, are the most diverse class of natural products appearing in a variety of natural sources, specifically in higher plants, and have a wide range of biological functions. This review describes novel or recent approaches and achievements in pathway engineering of Escherichia coli...

journal_title：Applied microbiology and biotechnology

authors： Harada H,Misawa N

更新日期：2009-10-01 00:00:00

abstract：:Metabolic stress is a phenomenon often discussed in conjunction with recombinant protein production in Escherichia coli. This investigation shows how heterologous protein production and the presence of host cell proteases is related to: (1) Isopropyl-beta- D-thiogalactopyranoside (IPTG) induction, (2) cell-mass concen...

journal_title：Applied microbiology and biotechnology

authors： Ramchuran SO,Nordberg Karlsson E,Velut S,De Maré L,Hagander P,Holst O

更新日期：2002-12-01 00:00:00

abstract：:Bioconversion of cassava-derived glucose to 2-keto-D-gluconic acid (2-KDG) using resting cells of immobilized Pseudomonas aeruginosa IFO 3448 was investigated. The tuberous roots of cassava were selected as the feedstock as they are inexpensive and widely available, and possess high amounts of starch (approximately 70...

journal_title：Applied microbiology and biotechnology

authors： Chia M,Van Nguyen TB,Choi WJ

更新日期：2008-04-01 00:00:00

abstract：:Despite the known hazardous effects of antimony (Sb) on human health, Sb monitoring biosensors have not been as actively investigated as arsenic (As) biosensors. Whole-cell bioreporters (WCBs) employing an arsenic-responsive operon and a regulatory protein (ArsR) are reportedly capable of monitoring arsenite, arsenate...

journal_title：Applied microbiology and biotechnology

authors： Lee W,Kim H,Jang G,Kim BG,Yoon Y

更新日期：2020-03-01 00:00:00

abstract：:Clostridium tyrobutyricum is a promising organism for butyrate and n-butanol production, but cannot grow on sucrose. Three genes (scrA, scrB, and scrK) involved in the sucrose catabolic pathway, along with an aldehyde/alcohol dehydrogenase gene, were cloned from Clostridium acetobutylicum and introduced into C. tyrobu...

journal_title：Applied microbiology and biotechnology

authors： Zhang J,Yu L,Xu M,Yang ST,Yan Q,Lin M,Tang IC

更新日期：2017-05-01 00:00:00

abstract：:In the course of more than 60-year history, penicillin G acylase (PGA) gained a unique position among enzymes used by pharmaceutical industry for production of β-lactam antibiotics. Kinetically controlled enzymatic syntheses of cephalosporins of novel generations in which PGA catalyzes coupling of activated acyl donor...

journal_title：Applied microbiology and biotechnology

authors： Marešová H,Plačková M,Grulich M,Kyslík P

更新日期：2014-04-01 00:00:00

abstract：:The genetic improvement of winemaking yeasts is a virtually infinite process, as the design of new strains must always cope with varied and ever-evolving production contexts. Good wine yeasts must feature both good primary traits, which are related to the overall fermentative fitness of the strain, and secondary trait...

journal_title：Applied microbiology and biotechnology

authors： Bonciani T,De Vero L,Mezzetti F,Fay JC,Giudici P

更新日期：2018-03-01 00:00:00

abstract：:Several studies support the use of probiotics for the treatment of minor gastrointestinal problems in infants. Positive effects on newborn colics have been evidenced after administration of Lactobacillus strains, whereas no studies have been reported regarding the use of bifidobacteria for this purpose. This work was ...

journal_title：Applied microbiology and biotechnology

authors： Aloisio I,Santini C,Biavati B,Dinelli G,Cencič A,Chingwaru W,Mogna L,Di Gioia D

更新日期：2012-12-01 00:00:00