当前位置： SCI文献检索 > APPLIED MICROBIOLOGY AND BIOTECHNOLOGY期刊下所有文献 > Biosynthesis of mono-acylated mannosylerythritol lipid in an acyltransferase gene-disrupted mutant of Pseudozyma tsukubaensis.

Biosynthesis of mono-acylated mannosylerythritol lipid in an acyltransferase gene-disrupted mutant of Pseudozyma tsukubaensis.

Abstract：

:The basidiomycetous yeast genus Pseudozyma produce large amounts of mannosylerythritol lipids (MELs), which are biosurfactants. A few Pseudozyma strains produce mono-acylated MEL as a minor compound using excess glucose as the sole carbon source. Mono-acylated MEL shows higher hydrophilicity than di-acylated MEL and has great potential for aqueous applications. Recently, the gene cluster involved in the MEL biosynthesis pathway was identified in yeast. Here, we generated an acyltransferase (PtMAC2) deletion strain of P. tsukubaensis 1E5 with uracil auxotrophy as a selectable marker. A PtURA5-mutant with a frameshift mutation in PtURA5 was generated as a uracil auxotroph of strain 1E5 by ultraviolet irradiation on plate medium containing 5-fluoro-orotic acid (5-FOA). In the mutant, PtMAC2 was replaced with a PtURA5 cassette containing the 5' untranslated region (UTR) (2000 bp) and 3' UTR (2000 bp) of PtMAC2 by homologous recombination, yielding strain ΔPtMAC2. Based on TLC and NMR analysis, we found that ΔPtMAC2 accumulates MEL acylated at the C-2' position of the mannose moiety. These results indicate that PtMAC2p catalyzes acylation at the C-3' position of the mannose of MEL.

journal_name

Appl Microbiol Biotechnol

journal_title

Applied microbiology and biotechnology

authors

Saika A,Utashima Y,Koike H,Yamamoto S,Kishimoto T,Fukuoka T,Morita T

doi

10.1007/s00253-017-8698-2

subject

Has Abstract

pub_date

2018-02-01 00:00:00

pages

1759-1767

issue

4

eissn

0175-7598

issn

1432-0614

pii

10.1007/s00253-017-8698-2

journal_volume

102

pub_type

杂志文章

相关文献

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY文献大全
  • Single-culture aerobic granules with Acinetobacter calcoaceticus.

    abstract：:Aerobic granules are cultivated by a single bacterial strain, Acinetobacter calcoaceticus, in a sequencing batch reactor (SBR). This strain presents as a good phenol reducer and an efficient auto coagulator in the presence of phenol, mediated by heat-sensitive adhesins proteins. Stable 2.3-mm granules were formed in t...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-007-1325-x

    authors： Adav SS,Lee DJ

    更新日期：2008-03-01 00:00:00

  • Aerobic biodegradation of selected monoterpenes.

    abstract：:Batch experiments were conducted to assess the biotransformation potential of four hydrocarbon monoterpenes (d-limonene, alpha-pinene, gamma-terpinene, and terpinolene) and four alcohols (arbanol, linalool, plinol, and alpha-terpineol) under aerobic conditions at 23 degrees C. Both forest-soil extract and enriched cul...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s002530050770

    authors： Misra G,Pavlostathis SG,Perdue EM,Araujo R

    更新日期：1996-07-01 00:00:00

  • Mechanisms of coal solubilization by the deuteromycetes Trichoderma atroviride and Fusarium oxysporum

    abstract：:Three different mechanisms can be envisaged that are used by fungi to solubilize coal: the production of alkaline substances, the extrusion of chelators and, of special interest in the scope of biotechnology, the action of enzymes. Whether these mechanisms are operating separately or in various combinations has not ye...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s002530051486

    authors： Holker U,Ludwig S,Scheel T,Hofer M

    更新日期：1999-07-01 00:00:00

  • Spatial and temporal distribution of nitrite-dependent anaerobic methane-oxidizing bacteria in an intertidal zone of the East China Sea.

    abstract：:Nitrite-dependent anaerobic methane oxidation (N-DAMO), which couples anaerobic methane oxidation and nitrite reduction, is a recently discovered bioprocess coupling microbial nitrogen and carbon cycles. The discovery of this microbial process challenges the traditional knowledge of global methane sinks and nitrogen l...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-017-8521-0

    authors： Wang J,Shen L,He Z,Hu J,Cai Z,Zheng P,Hu B

    更新日期：2017-11-01 00:00:00

  • Production of edible mushrooms in forests: trends in development of a mycosilviculture.

    abstract：:Developing the production of ectomycorrhizal (ECM) mushrooms in forest has become a challenge. Only a few ECM species are currently cultivable. Controlled mycorrhization practices offer promising advance to produce currently uncultivable ECM mushrooms. The persistence of the production of edible species, either cultiv...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章,评审

    doi：10.1007/s00253-010-3022-4

    authors： Savoie JM,Largeteau ML

    更新日期：2011-02-01 00:00:00

  • Isolation of phenazine 1,6-di-carboxylic acid from Pseudomonas aeruginosa strain HRW.1-S3 and its role in biofilm-mediated crude oil degradation and cytotoxicity against bacterial and cancer cells.

    abstract：:Pseudomonas sp. has long been known for production of a wide range of secondary metabolites during late exponential and stationary phases of growth. Phenazine derivatives constitute a large group of secondary metabolites produced by microorganisms including Pseudomonas sp. Phenazine 1,6-di-carboxylic acid (PDC) is one...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-015-6707-x

    authors： Dasgupta D,Kumar A,Mukhopadhyay B,Sengupta TK

    更新日期：2015-10-01 00:00:00

  • Impact of protein uptake and degradation on recombinant protein secretion in yeast.

    abstract：:Protein titers, a key bioprocessing metric, depend both on the synthesis of protein and the degradation of protein. Secreted recombinant protein production in Saccharomyces cerevisiae is an attractive platform as minimal media can be used for cultivation, thus reducing fermentation costs and simplifying downstream pur...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-014-5783-7

    authors： Tyo KE,Liu Z,Magnusson Y,Petranovic D,Nielsen J

    更新日期：2014-08-01 00:00:00

  • Detection of two distinct substrate-dependent catabolic responses in yeast cells using a mediated electrochemical method.

    abstract：:Mediated electrochemical detection of catabolism in prokaryotic cells is well documented; however, the application of this technique to eukaryotic cells has received less attention. Two catabolic substrate-dependent mediated electrochemical signals were detected in the yeast Saccharomyces cerevisiae. The signal using ...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-002-1108-3

    authors： Baronian KH,Downard AJ,Lowen RK,Pasco N

    更新日期：2002-10-01 00:00:00

  • Biofabrication: an overview of the approaches used for printing of living cells.

    abstract：:The development of cell printing is vital for establishing biofabrication approaches as clinically relevant tools. Achieving this requires bio-inks which must not only be easily printable, but also allow controllable and reproducible printing of cells. This review outlines the general principles and current progress a...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章,评审

    doi：10.1007/s00253-013-4853-6

    authors： Ferris CJ,Gilmore KG,Wallace GG,In het Panhuis M

    更新日期：2013-05-01 00:00:00

  • Microbial oxidation of ebastine.

    abstract：:The microbial oxidation of ebastine to carebastine was investigated. Among the 15 micro-organisms examined, only the Cunninghamella strains showed the desired biotransformation. Cunninghamella blakesleeana oxidised the substrate within 7 days, via the intermediates alcohol and aldehyde, mainly to carebastine, the corr...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/BF00178610

    authors： Schwartz H,Liebig-Weber A,Hochstätter H,Böttcher H

    更新日期：1996-02-01 00:00:00

  • ATP-dependent fructose uptake system in Deinococcus radiodurans.

    abstract：:The bacterial phosphoenolpyruvate (PEP)-dependent group translocation system (PTS) requires the presence of both membrane-bound and cytoplasmic components to phosphorylate and translocate sugar. Deinococcus radiodurans has a functional fruA gene coding for the membrane-bound components of the fructose-specific PTS. Ho...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-011-3514-x

    authors： Lee HY,Magotra M,Wong TY,Chakraborty C,Liu JK

    更新日期：2012-02-01 00:00:00

  • Transcriptional analysis of genes for energy catabolism and hydrolytic enzymes in the filamentous fungus Aspergillus oryzae using cDNA microarrays and expressed sequence tags.

    abstract：:Aspergillus oryzae is a fungus used extensively in the fermentation industry. We constructed cDNA microarrays comprising 2,070 highly expressed cDNAs selected from the approximately 6,000 non-redundant expressed sequence tags (ESTs) in the A. oryzae EST database (http://www.aist.go.jp/RIODB/ffdb/index.html). Using the...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-004-1608-4

    authors： Maeda H,Sano M,Maruyama Y,Tanno T,Akao T,Totsuka Y,Endo M,Sakurada R,Yamagata Y,Machida M,Akita O,Hasegawa F,Abe K,Gomi K,Nakajima T,Iguchi Y

    更新日期：2004-07-01 00:00:00

  • Comparison of the biological activities of Saccharomyces cerevisiae-expressed intracellular EGF, extracellular EGF, and tagged EGF in early-weaned pigs.

    abstract：:Epidermal growth factor (EGF) ameliorates stress and prevents incomplete gastrointestinal development in early-weaned piglets in commercial swine farming. This study aimed to further analyze the biological activities of intracellularly expressed EGF (IE-EGF), extracellularly expressed EGF (EE-EGF), and tagged EGF (T-E...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-015-6468-6

    authors： Wang S,Guo C,Zhou L,Zhang Z,Huang Y,Yang J,Bai X,Yang K

    更新日期：2015-09-01 00:00:00

  • Simultaneous P-solubilizing and biocontrol activity of microorganisms: potentials and future trends.

    abstract：:Phosphate (P)-solubilizing microorganisms as a group form an important part of the microorganisms, which benefit plant growth and development. Growth promotion and increased uptake of phosphate are not the only mechanisms by which these microorganisms exert a positive effect on plants. Microbially mediated solubilizat...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章,评审

    doi：10.1007/s00253-006-0380-z

    authors： Vassilev N,Vassileva M,Nikolaeva I

    更新日期：2006-06-01 00:00:00

  • Polychlorinated biphenyl (PCB) degradation and persistence of a gfp-marked Ralstonia eutropha H850 in PCB-contaminated soil.

    abstract：:Ralstonia eutropha H850 was labelled chromosomally with a gfp marker gene encoding for the green fluorescent protein, and designated R. eutropha H850g13. Visual observation of green fluorescent cells under an epifluorescence microscope, and PCR amplification products, confirmed that the bacterium was labelled with gfp...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-003-1380-x

    authors： Abbey AM,Beaudette LA,Lee H,Trevors JT

    更新日期：2003-12-01 00:00:00

  • Studies on the inactivation of the flavoprotein D-amino acid oxidase from Trigonopsis variabilis.

    abstract：:Inactivation of D-amino acid oxidase occurred by different mechanisms. The enzyme showed a rapid loss of activity in the presence of micromolar amounts of Cu2+ and Hg2+. It was also sensitive to oxidative inactivation by Fe2+ and H2O2 when both reagents were added in millimolar amounts. When oxidatively inactivated D-...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/BF00578456

    authors： Schräder T,Andreesen JR

    更新日期：1996-05-01 00:00:00

  • Cell surface-engineered yeast displaying a histidine oligopeptide (hexa-His) has enhanced adsorption of and tolerance to heavy metal ions.

    abstract：:A histidine oligopeptide (hexa-His) with the ability to chelate divalent heavy metal ions was displayed on the yeast cell surface for the purpose of enhanced adsorption of heavy metal ions. We genetically fused a hexa-His-encoding gene with the gene encoding the C-terminal half of alpha-agglutinin that includes a glyc...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s002530100813

    authors： Kuroda K,Shibasaki S,Ueda M,Tanaka A

    更新日期：2001-12-01 00:00:00

  • Genetic reidentification of the pectinolytic yeast strain SCPP as Saccharomyces bayanus var. uvarum.

    abstract：:Using genetic hybridization analysis, pulsed-field gel electrophoresis of chromosomal DNA and PCR/RFLP analysis of the MET2 gene, we reidentified 11 Champagne yeast strains. Two of them, SCPP and SC4, were found to belong to Saccharomyces bayanus var. uvarum and the remaining strains to S. cerevisiae. Strain ...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s002530000480

    authors： Naumov GI,Naumova ES,Aigle M,Masneuf I,Belarbi A

    更新日期：2001-01-01 00:00:00

  • Efficient production of deoxynucleoside-5'-monophosphates using deoxynucleoside kinase coupled with a GTP-regeneration system.

    abstract：:Deoxynucleoside-5'-monophosphates (5'-dNMPs) are the basic components of DNA and are widely used in medicine and as chemical and biochemical reagents. A large amount of effort has been expended to obtain 5'-dNMPs of high quality and at a low cost. However, these procedures are inefficient and inconvenient. In this stu...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-013-5173-6

    authors： Zou Z,Ding Q,Ou L,Yan B

    更新日期：2013-11-01 00:00:00

  • Heterologous expression of the benzoate para-hydroxylase encoding gene (CYP53B1) from Rhodotorula minuta by Yarrowia lipolytica.

    abstract：:There is currently an increasing number of cytochrome P450 (CYP450) monooxygenase encoding genes becoming available from various genome-sequencing projects. These enzymes require association with cytochrome P450 reductase (CPR) to achieve optimal activities. In this study, the CYP53B1 gene, which encodes a benzoate pa...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-005-0264-7

    authors： Shiningavamwe A,Obiero G,Albertyn J,Nicaud JM,Smit M

    更新日期：2006-09-01 00:00:00

  • Biodegradation of textile azo dye by Shewanella decolorationis S12 under microaerophilic conditions.

    abstract：:The complete biodegradation of azo dye, Fast Acid Red GR, was observed under microaerophilic conditions by Shewanella decolorationis S12. Although the highest decolorizing rate was measured under anaerobic condition and the highest biomass was obtained under aerobic condition, a further biodegradation of decolorizing ...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-007-1032-7

    authors： Xu M,Guo J,Sun G

    更新日期：2007-09-01 00:00:00

  • Development of chitosan-coated agar-gelatin particles for probiotic delivery and targeted release in the gastrointestinal tract.

    abstract：:This study reports the development of a novel and simple formulation for probiotic delivery using chitosan-coated agar-gelatin gel particles. This methodology involves the production of agar-gelatin particles by thermally treating a mixture of agar and gelatin solutions at high temperatures (121 °C) and subsequently c...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-020-10632-w

    authors： Albadran HA,Monteagudo-Mera A,Khutoryanskiy VV,Charalampopoulos D

    更新日期：2020-07-01 00:00:00

  • Screening, identification, and characterization of a novel saccharide-stimulated β-glycosidase from a soil metagenomic library.

    abstract：:MeBglD2, a β-glycosidase that is highly activated in the presence of various monosaccharides and disaccharides, was isolated from a soil metagenomic library. MeBglD2 had not only β-glucosidase activity but also β-galactosidase and β-fucosidase activities. MeBglD2 β-glucosidase activity increased in a cellobiose concen...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-016-7803-2

    authors： Matsuzawa T,Yaoi K

    更新日期：2017-01-01 00:00:00

  • Review of research on Dendrobium, a prized folk medicine.

    abstract：:Medicinal plants of the Dendrobium genus are highly prized, and hence, methodologies have been developed to authenticate Dendrobium drugs from its adulterants. Many bioactive constituents of Dendrobium species have been identified. The macromolecules included lectins; the enzymes chalcone synthase, sucrose synthase, a...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章,评审

    doi：10.1007/s00253-011-3829-7

    authors： Ng TB,Liu J,Wong JH,Ye X,Wing Sze SC,Tong Y,Zhang KY

    更新日期：2012-03-01 00:00:00

  • Tandem repeat-tRNA (TRtRNA) PCR method for the molecular typing of non-Saccharomyces subspecies.

    abstract：:There is a worldwide trend to understand the impact of non-Saccharomyces yeast species on the process of winemaking. Although the predominant species at the end of the fermentation is Saccharomyces cerevisiae, several non-Saccharomyces species present during the first days of the process can produce and/or release aro...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-011-3714-4

    authors： Barquet M,Martín V,Medina K,Pérez G,Carrau F,Gaggero C

    更新日期：2012-01-01 00:00:00

  • Gene cloning and overexpression of two conjugated polyketone reductases, novel aldo-keto reductase family enzymes, of Candida parapsilosis.

    abstract：:The genes encoding two conjugated polyketone reductases (CPR-C1, CPR-C2) of Candida parapsilosis IFO 0708 were cloned and sequenced. The genes encoded a total of 304 and 307 amino acid residues for CPR-C1 and CPR-C2, respectively. The deduced amino acid sequences of the two enzymes showed high similarity to each other...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-003-1484-3

    authors： Kataoka M,Delacruz-Hidalgo AR,Akond MA,Sakuradani E,Kita K,Shimizu S

    更新日期：2004-04-01 00:00:00

  • Construction of first-generation lactococcal integrative cloning vectors.

    abstract：:Using a randomly-cloned, HindIII-digested, chromosomal fragment from Lactococcus lactis subsp. lactis LM0230, first-generation lactococcal integrative cloning vectors were developed. Through dideoxy DNA sequence analysis, the cloned chromosomal DNA fragment was determined to be 1026 base pairs. Southern hybridization ...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/BF00170391

    authors： McIntyre DA,Harlander SK

    更新日期：1993-11-01 00:00:00

  • Optimizing biodegradation of phenanthrene dissolved in nonaqueous-phase liquids.

    abstract：:A study was conducted to optimize the biodegradation in soil slurries of phenanthrene initially dissolved in nonaqueous-phase liquids (NAPLs). The slow rate of degradation of phenanthrene in dibutyl phthalate was increased by addition of phenanthrene-degrading microorganisms to soil slurries containing the NAPL. The r...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s002530050682

    authors： Birman I,Alexander M

    更新日期：1996-03-01 00:00:00

  • Simultaneous degradation of chloro- and methyl-substituted aromatic compounds: competition between Pseudomonas strains using the ortho and meta pathway or the ortho pathway exclusively.

    abstract：:Pseudomonas sp. D7-4 and Pseudomonas sp. B13 FR1(pFRC20P) degraded mixtures of chloro- and methyl-substituted benzoates exclusively via an extended ortho pathway, whereas in Pseudomonas putida WR 201 both ortho and meta fission were induced by mixtures of 3-chloro- and 3-methylbenzoate or even by 3-chloro-benzoate alo...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s002530051282

    authors： Franck-Mokross AC,Schmidt E

    更新日期：1998-08-01 00:00:00

  • Stable transformation of Chromobacterium violaceum with a broad-host-range plasmid.

    abstract：:Stable transformants of Chromobacterium violaceum were obtained by high-voltage electroporation with a 7-kilobase binary plasmid. The technique was reliable, reproducible, and simple, with efficiencies of 10(5) transformants/microg of plasmid DNA. The electrical conditions that resulted in the highest efficiencies wer...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-005-0140-5

    authors： Broetto L,Cecagno R,Sant'anna FH,Weber S,Schrank IS

    更新日期：2006-07-01 00:00:00