Construction of first-generation lactococcal integrative cloning vectors.

abstract：:For the fermentative production of plant-specific flavanones (naringenin, pinocembrin) by Escherichia coli, a plasmid was constructed which carried an artificial biosynthetic gene cluster, including PAL encoding a phenylalanine ammonia-lyase from a yeast, ScCCL encoding a cinnamate/coumarate:CoA ligase from the actino...

journal_title：Applied microbiology and biotechnology

authors： Miyahisa I,Kaneko M,Funa N,Kawasaki H,Kojima H,Ohnishi Y,Horinouchi S

更新日期：2005-09-01 00:00:00

abstract：:There is emerging interest in the anaerobic degradation of crude oil. However, there is limited knowledge about the geochemical effects and microbiological activities for it. A mixture of anaerobic sludge and the production water from an oil well was used as an inoculum to construct four consortia, which were incubate...

journal_title：Applied microbiology and biotechnology

authors： Xiong S,Li X,Chen J,Zhao L,Zhang H,Zhang X

更新日期：2015-02-01 00:00:00

abstract：:Edwardsiella tarda is a leading fish pathogen haunting worldwide aquaculture industry. In E. tarda, two-component system EsrA-EsrB positively regulates type III and VI secretion systems (T3SS and T6SS) and negatively regulates hemolysin EthA, which has been demonstrated to be essential for the invasion processes in fi...

journal_title：Applied microbiology and biotechnology

authors： Yang W,Wang L,Zhang L,Qu J,Wang Q,Zhang Y

更新日期：2015-02-01 00:00:00

abstract：:Several studies support the use of probiotics for the treatment of minor gastrointestinal problems in infants. Positive effects on newborn colics have been evidenced after administration of Lactobacillus strains, whereas no studies have been reported regarding the use of bifidobacteria for this purpose. This work was ...

journal_title：Applied microbiology and biotechnology

authors： Aloisio I,Santini C,Biavati B,Dinelli G,Cencič A,Chingwaru W,Mogna L,Di Gioia D

更新日期：2012-12-01 00:00:00

abstract：:A maltotriose-producing α-amylase, AmyA, from a newly isolated bacterial strain Microbulbifer thermotolerans DAU221 was purified and characterized in the heterologous host, Escherichia coli, using the pCold I vector. The amyA gene encoded a 761-residue protein composed of a 33 amino acid secretion signal peptide. The ...

journal_title：Applied microbiology and biotechnology

authors： Lee YS,Park DJ,Choi YL

更新日期：2015-05-01 00:00:00

abstract：:The production of botulinum neurotoxin A (BoNT/A) for therapeutic and cosmetic applications requires precise determination of batch potency, and the enzymatic activity of BoNT/A light chain is a crucial index that can be measured in vitro. We previously established a SNAP-25 chip-based assay using surface plasmon reso...

journal_title：Applied microbiology and biotechnology

authors： Lévêque C,Ferracci G,Maulet Y,Grand-Masson C,Seagar M,El Far O

更新日期：2015-05-01 00:00:00

abstract：:Substrate oxidation of aromatic substances by the enzyme laccase followed by a heteromolecular coupling with a co-substrate is a promising possibility for the synthesis of new compounds. To find a suitable reactor for the effective production of new compounds, the laccase-catalysed coupling of 3-(3,4-dihydroxyphenyl)p...

journal_title：Applied microbiology and biotechnology

authors： Pilz R,Hammer E,Schauer F,Kragl U

更新日期：2003-02-01 00:00:00

abstract：:Various single-cell isolation techniques, including dilution, micromanipulation, flow cytometry, microfluidics, and compartmentalization, have been developed. These techniques can be used to cultivate previously uncultured microbes, to assess and monitor cell physiology and function, and to screen for novel microbiolo...

journal_title：Applied microbiology and biotechnology

authors： Ishii S,Tago K,Senoo K

更新日期：2010-05-01 00:00:00

abstract：:Potato pulp is a poorly utilized, high-volume co-processing product resulting from industrial potato starch manufacturing. Potato pulp mainly consists of the tuber plant cell wall material and is particularly rich in pectin, notably galactan branched rhamnogalacturonan I type pectin which has previously been shown to ...

journal_title：Applied microbiology and biotechnology

authors： Thomassen LV,Vigsnæs LK,Licht TR,Mikkelsen JD,Meyer AS

更新日期：2011-05-01 00:00:00

abstract：:The lipids (fats and oils) degradation capabilities of soil microorganisms were investigated for possible application in treatment of lipids-contaminated wastewater. We isolated a strain of the bacterium Raoultella planticola strain 232-2 that is capable of efficiently catabolizing lipids under acidic conditions such ...

journal_title：Applied microbiology and biotechnology

authors： Sugimori D,Watanabe M,Utsue T

更新日期：2013-01-01 00:00:00

abstract：:Rhodococcus erythropolis U23A is a polychlorinated biphenyl (PCB)-degrading bacterium isolated from the rhizosphere of plants grown on a PCB-contaminated soil. Strain U23A bphA exhibited 99% identity with bphA1 of Rhodococcus globerulus P6. We grew Arabidopsis thaliana in a hydroponic axenic system, collected, and con...

journal_title：Applied microbiology and biotechnology

authors： Toussaint JP,Pham TT,Barriault D,Sylvestre M

更新日期：2012-09-01 00:00:00

abstract：:A bacterial biofilm, capable of mineralising a technical mixture of cis- and trans-1,3-dichloropropene (DCPE), was enriched on the biomedium side of an extractive membrane biofilm reactor (EMBR). The membrane separates the biomedium from the industrial waste water, in terms of pH, ionic strength and the concentration ...

journal_title：Applied microbiology and biotechnology

authors： Katsivela E,Bonse D,Krüger A,Strömpl C,Livingston A,Wittich RM

更新日期：1999-11-01 00:00:00

abstract：:Glucosylglycerols (GGs) are known as compatible solutes accumulated by some bacteria including cyanobacteria as well as higher plants for their adaptations to salt or desiccation stresses. Since being identified in Japanese sake, their physiological effects and potential applications on human health cares have been ex...

journal_title：Applied microbiology and biotechnology

authors： Tan X,Luo Q,Lu X

更新日期：2016-07-01 00:00:00

abstract：:The recent use of photosynthetic organisms such as Chlamydomonas reinhardtii in biomedical applications has demonstrated their potential for the treatment of acute and chronic tissue hypoxia. Moreover, transgenic microalgae have been suggested as an alternative in situ drug delivery system. In this study, we set out t...

journal_title：Applied microbiology and biotechnology

authors： Jarquín-Cordero M,Chávez MN,Centeno-Cerdas C,Bohne AV,Hopfner U,Machens HG,Egaña JT,Nickelsen J

更新日期：2020-01-01 00:00:00

abstract：:Cyanobacterial mutants defective in acyl-acyl carrier protein synthetase (Aas) secrete free fatty acids (FFAs) into the external medium and hence have been used for the studies aimed at photosynthetic production of biofuels. While the wild-type strain of Synechocystis sp. PCC 6803 is highly sensitive to exogenously ad...

journal_title：Applied microbiology and biotechnology

authors： Kojima K,Keta S,Uesaka K,Kato A,Takatani N,Ihara K,Omata T,Aichi M

更新日期：2016-12-01 00:00:00

abstract：:Cells of an aerobic three-membered bacterial co-culture, designated as ECO3, capable of cometabolizing and aerobically dechlorinating low-chlorinated biphenyls in the presence of biphenyl, were immobilized on Manville silica beads, on frosted-glass beads and on polyurethane foam cubes in packed-bed bioreactors continu...

journal_title：Applied microbiology and biotechnology

authors： Fava F,Di Gioia D,Marchetti L,Quattroni G

更新日期：1996-05-01 00:00:00

abstract：:Soil microbial autotrophs play a significant role in CO2 fixation in terrestrial ecosystem, particularly in vegetation-constrained ecosystems with environmental stresses, such as the Tibetan Plateau characterized by low temperature and high UV. However, soil microbial autotrophic communities and their driving factors ...

journal_title：Applied microbiology and biotechnology

authors： Guo G,Kong W,Liu J,Zhao J,Du H,Zhang X,Xia P

更新日期：2015-10-01 00:00:00

abstract：:Nonadapted freshwater sediment slurries and sediment slurries adapted to dechlorinate 2,3-dichloropyridine (2,3-Cl2Pyd), 2,3-dichloroaniline (2,3-Cl2Anl), 2,3-dichlorophenol (2,3-Cl2PhOH), 3,5-dichloropyridine (3,5-Cl2Pyd), 3,5-dichloroaniline (3,5-Cl2Anl) and 3,5-dichlorophenol (3,5-Cl2PhOH) were studied to determine...

journal_title：Applied microbiology and biotechnology

authors： Liu SM,Jones WJ

更新日期：1995-08-01 00:00:00

abstract：:In this work, the production of 1,3-propanediol from glucose and molasses was studied in a two-step process using two recombinant microorganisms. The first step of the process is the conversion of glucose or other sugar into glycerol by the metabolic engineered Saccharomyces cerevisiae strain HC42 adapted to high (>20...

journal_title：Applied microbiology and biotechnology

authors： Mendes FS,González-Pajuelo M,Cordier H,François JM,Vasconcelos I

更新日期：2011-11-01 00:00:00

abstract：:A novel fluoro-containing jasmonate derivative was chemically synthesized and evaluated as a potential elicitor with respect to the induction of plant defense responses and the biosynthesis of plant secondary metabolites. A bioactive taxuyunnanine C (Tc)-producing cell line of Taxus chinensis was taken as a model plan...

journal_title：Applied microbiology and biotechnology

authors： Qian ZG,Zhao ZJ,Xu Y,Qian X,Zhong JJ

更新日期：2005-07-01 00:00:00

abstract：:Acid phosphatase production by recombinant Arxula adeninivorans was carried out in submerged fermentation. Using the Plackett-Burman design, three fermentation variables (pH, sucrose concentration, and peptone concentration) were identified to significantly affect acid phosphatase and biomass production, and these wer...

journal_title：Applied microbiology and biotechnology

authors： Minocha N,Kaur P,Satyanarayana T,Kunze G

更新日期：2007-08-01 00:00:00

abstract：:Possessing three circular chromosomes is a distinct genomic characteristic of Burkholderia cenocepacia AU 1054, a clinically important pathogen in cystic fibrosis. In this study, base composition, codon usage and functional role category were analyzed in the B. cenocepacia AU 1054 genome. Although no bias in the base ...

journal_title：Applied microbiology and biotechnology

authors： Yuan J,Yang M,Ren J,Fu B,Jiang F,Zhang X

更新日期：2014-01-01 00:00:00

abstract：:Epoxide hydrolases (EHs) of fungal origin have the ability to catalyze the enantioselective hydrolysis of epoxides to their corresponding diols. However, wild type fungal EHs are limited in substrate range and enantioselectivity. Additionally, the production of fungal epoxide hydrolase (EH) by wild-type strains is typ...

journal_title：Applied microbiology and biotechnology

authors： Maharajh D,Roth R,Lalloo R,Simpson C,Mitra R,Görgens J,Ramchuran S

更新日期：2008-05-01 00:00:00

abstract：:The ω-3 fatty acid desaturase (ω3Des) is a key enzyme in the biosynthesis of polyunsaturated fatty acids (PUFAs). However, the enzyme exhibits a significant preference towards different fatty acid substrates. To examine the molecular mechanism of its substrate specificity, a series of site-directed mutants were constr...

journal_title：Applied microbiology and biotechnology

authors： Rong C,Chen H,Wang M,Gu Z,Zhao J,Zhang H,Chen W,Chen YQ

更新日期：2018-11-01 00:00:00

abstract：:AmyC, a glycoside hydrolase family 57 (GH57) enzyme of Thermotoga maritima MSB8, has previously been identified as an intracellular α-amylase playing a role in either maltodextrin utilization or storage polysaccharide metabolism. However, the α-amylase specificity of AmyC is questionable as extensive phylogenetic anal...

journal_title：Applied microbiology and biotechnology

authors： Zhang X,Leemhuis H,Janeček Š,Martinovičová M,Pijning T,van der Maarel MJEC

更新日期：2019-08-01 00:00:00

abstract：:Cultures of Microcoleus consortia polluted with two different types of crude oil, one with high content in aliphatic hydrocarbons (Casablanca) and the other rich in sulphur and aromatic compounds (Maya), were grown for 50 days and studied for changes in oil composition. No toxic effects from these oils were observed o...

journal_title：Applied microbiology and biotechnology

authors： De Oteyza TG,Grimalt JO,Diestra E,Solé A,Esteve I

更新日期：2004-12-01 00:00:00

abstract：:Environmentally friendly control measures are needed for suppression of soilborne pathogens of vegetable crops in the Republic of Korea. In vitro challenge assays were used to screen approximately 500 bacterial isolates from 20 Korean greenhouse soils for inhibition of diverse plant pathogens. One isolate, Bacillus su...

journal_title：Applied microbiology and biotechnology

authors： Chung S,Kong H,Buyer JS,Lakshman DK,Lydon J,Kim SD,Roberts DP

更新日期：2008-08-01 00:00:00

abstract：:Corynebacterium glutamicum shows a great potential for the production of the polyamide monomer putrescine (1,4-diaminobutane). Previously, we constructed the putrescine-producing strain PUT1 by deletion of argF, the gene for ornithine transcarbamoylase (OTC), and argR, encoding the L-arginine repressor, combined with ...

journal_title：Applied microbiology and biotechnology

authors： Schneider J,Eberhardt D,Wendisch VF

更新日期：2012-07-01 00:00:00

abstract：:In this work, fermentation and formulation aspects of the nematophagous fungus Hirsutella rhossiliensis BBA were investigated. When incubated in 2% (w/w) glucose and 0.5% (w/w) yeast extract medium in a 1-L Erlenmeyer flask without baffles, heavy pellet formation was observed. Only 40% of the mycelium had a size less ...

journal_title：Applied microbiology and biotechnology

authors： Patel AV,Jakobs-Schönwandt D,Rose T,Vorlop KD

更新日期：2011-03-01 00:00:00

abstract：:Betalamic acid is the structural unit of all the natural pigments betalains. These are nitrogen-containing water-soluble compounds with high colorant and bioactive properties, characteristic of plants of the order Caryophyllales. The formation of betalamic acid from the precursor amino acid 3,4-dihydroxy-L-phenylalani...

journal_title：Applied microbiology and biotechnology

authors： Gandía-Herrero F,García-Carmona F

更新日期：2014-02-01 00:00:00