A chip-based assay for botulinum neurotoxin A activity in pharmaceutical preparations.

abstract：:The aim of the present study was to develop a new cell modification method to facilitate the cell separation from broth. In order to reduce the transfer limitation of substrate and product caused by general immobilization methods in the following biotransformation of glycerol, the carboxyl-functioned superparamagnetic...

journal_title：Applied microbiology and biotechnology

authors： Chen G,Chen J

更新日期：2013-05-01 00:00:00

abstract：:Wood contains primary extractives, which are present in all woods, and secondary extractives, which are confined in certain wood species. Extractives in wood play a major role in wood-bonding processes, as they can contribute to or determine the bonding relevant properties of wood such as acidity and wettability. Ther...

journal_title：Applied microbiology and biotechnology

authors： Roffael E

更新日期：2016-02-01 00:00:00

abstract：:Under specific environmental conditions, Pseudomonas aeruginosa produces a biodegradable surfactant rhamnolipid. Evidences suggest that this biosurfactant is involved in protecting cells against oxidative stress; however, the effects of oxidative stress on its production and other virulence factors are still unclear. ...

journal_title：Applied microbiology and biotechnology

authors： Pacheco GJ,Reis RS,Fernandes AC,da Rocha SL,Pereira MD,Perales J,Freire DM

更新日期：2012-09-01 00:00:00

abstract：:Lactic acid is a versatile organic acid, which finds major application in the food, pharmaceuticals, and chemical industries. Microbial fermentation has the advantage that by choosing a strain of lactic acid bacteria producing only one of the isomers, an optically pure product can be obtained. The production of L: (+)...

journal_title：Applied microbiology and biotechnology

authors： Panesar PS,Kennedy JF,Knill CJ,Kosseva MR

更新日期：2007-02-01 00:00:00

abstract：:Aerobic granules are cultivated by a single bacterial strain, Acinetobacter calcoaceticus, in a sequencing batch reactor (SBR). This strain presents as a good phenol reducer and an efficient auto coagulator in the presence of phenol, mediated by heat-sensitive adhesins proteins. Stable 2.3-mm granules were formed in t...

journal_title：Applied microbiology and biotechnology

authors： Adav SS,Lee DJ

更新日期：2008-03-01 00:00:00

abstract：:A novel β-glucosidase from Penicillium aculeatum was purified as a single 110.5-kDa band on SDS-PAGE with a specific activity of 75.4 U mg⁻¹ by salt precipitation and Hi-Trap Q HP and Resource Q ion exchange chromatographies. The purified enzyme was identified as a member of the glycoside hydrolase 3 family based on i...

journal_title：Applied microbiology and biotechnology

authors： Lee GW,Yoo MH,Shin KC,Kim KR,Kim YS,Lee KW,Oh DK

更新日期：2013-07-01 00:00:00

abstract：:L-Malic acid and fumaric acid are C4 dicarboxylic organic acids and considered as promising chemical building blocks. They can be applied as food preservatives and acidulants in rust removal and as polymerization starter units. Molds of the genus Aspergillus are able to produce malic acid in large quantities from gluc...

journal_title：Applied microbiology and biotechnology

authors： Ochsenreither K,Fischer C,Neumann A,Syldatk C

更新日期：2014-06-01 00:00:00

abstract：:Developing the production of ectomycorrhizal (ECM) mushrooms in forest has become a challenge. Only a few ECM species are currently cultivable. Controlled mycorrhization practices offer promising advance to produce currently uncultivable ECM mushrooms. The persistence of the production of edible species, either cultiv...

journal_title：Applied microbiology and biotechnology

authors： Savoie JM,Largeteau ML

更新日期：2011-02-01 00:00:00

abstract：:The Bacillus subtilis lipoprotein PrsA enhances the yield of several homologous and heterologous exported proteins in B. subtilis by being involved in the posttranslocational stage of the secretion process. In this work, we have studied the effect of B. subtilis PrsA on the secretion of Bacillus amyloliquefaciens alph...

journal_title：Applied microbiology and biotechnology

authors： Lindholm A,Ellmén U,Tolonen-Martikainen M,Palva A

更新日期：2006-12-01 00:00:00

abstract：:Cultivation of Aspergillus terreus ATCC 20542 in a stirred tank bioreactor was performed to induce the biosynthesis of secondary metabolites and provide the bioprocess-related insights into the metabolic capabilities of the investigated strain. The activation of biosynthetic routes was attempted by the diversification...

journal_title：Applied microbiology and biotechnology

authors： Boruta T,Bizukojc M

更新日期：2016-04-01 00:00:00

abstract：:Fungal interspecific interactions enhance biosynthesis of phenylpropanoid metabolites (PM), and production of nitric oxide (NO) is known to be involved in this process. However, it remains unknown which signaling pathway(s) or regulator(s) mediate fungal PM biosynthesis. In this study, we cocultured two white-rot fung...

journal_title：Applied microbiology and biotechnology

authors： Zhao Y,Xi Q,Xu Q,He M,Ding J,Dai Y,Keller NP,Zheng W

更新日期：2015-05-01 00:00:00

abstract：:A novel cell surface display system in Aspergillus oryzae was established by using a chitin-binding module (CBM) from Saccharomyces cerevisiae as an anchor protein. CBM was fused to the N or C terminus of green fluorescent protein (GFP) and the fusion proteins (GFP-CBM and CBM-GFP) were expressed using A. oryzae as a ...

journal_title：Applied microbiology and biotechnology

authors： Tabuchi S,Ito J,Adachi T,Ishida H,Hata Y,Okazaki F,Tanaka T,Ogino C,Kondo A

更新日期：2010-08-01 00:00:00

abstract：:Grasslands in semi-arid Northern China are widely desertified, thus inducing the formation of a large area of moving sand lands. Revegetation of the sandy land is commonly adopted to restore degraded grasslands. The structure of the soil microbial community might dramatically change during degradation and recovery bec...

journal_title：Applied microbiology and biotechnology

authors： Cao C,Zhang Y,Cui Z,Feng S,Wang T,Ren Q

更新日期：2017-08-01 00:00:00

abstract：:Actinomycetes are the most important producers of secondary metabolites for medical, agricultural and industrial applications. Efficient engineering of bacterial genomes to improve their biosynthetic capabilities largely depends on the available arsenal of tools and vectors. One of the most widely used vector systems ...

journal_title：Applied microbiology and biotechnology

authors： Muth G

更新日期：2018-11-01 00:00:00

abstract：:Because engineering of the 101.016-bp megaplasmid pKB1 of Gordonia westfalica Kb1 failed due to the absence of an effective transfer system, pKB1 was transferred by conjugation from G. westfalica Kb1 to a kanamycin-resistant mutant of Rhodococcus opacus PD630 at a frequency of about 6.2 x 10(-8) events per recipient c...

journal_title：Applied microbiology and biotechnology

authors： Bröker D,Arenskötter M,Steinbüchel A

更新日期：2008-01-01 00:00:00

abstract：:5-Hydroxytryptophan (5-HTP) is the precursor of the neurotransmitter serotonin and has been used for the treatment of various diseases such as depression, insomnia, chronic headaches, and binge eating associated obesity. The production of 5-HTP had been achieved in our previous report, by the development of a recombin...

journal_title：Applied microbiology and biotechnology

authors： Xu D,Fang M,Wang H,Huang L,Xu Q,Xu Z

更新日期：2020-03-01 00:00:00

abstract：:The genome of the coprophilous fungus Podospora anserina displays an impressive array of genes encoding hemicellulolytic enzymes. In this study, we focused on a putative carbohydrate esterase (CE) from family 16 (CE16) that bears a carbohydrate-binding module from family CBM1. The protein was heterologously expressed ...

journal_title：Applied microbiology and biotechnology

authors： Puchart V,Berrin JG,Haon M,Biely P

更新日期：2015-12-01 00:00:00

abstract：:Two-dimensional gel electrophoresis was conducted to investigate the effect of H2O2 on whole protein expression in Acinetobacter oleivorans DR1. Functional classification of 13 upregulated proteins using MALDI-TOF mass spectrometry showed relationships with oxidative stress, energy production and conversion, nucleotid...

journal_title：Applied microbiology and biotechnology

authors： Kim J,Cho Y,Jang IA,Park W

更新日期：2015-12-01 00:00:00

abstract：:Functional screening for lipolytic enzymes at low temperatures resulted in the isolation of the novel cold-active esterases, EstM-N1 and EstM-N2, from a metagenomic DNA library of arctic soil samples. EstM-N1 and EstM-N2 were 395 and 407 amino acids in length, respectively, and showed the highest similarity to class C...

journal_title：Applied microbiology and biotechnology

authors： Yu EY,Kwon MA,Lee M,Oh JY,Choi JE,Lee JY,Song BK,Hahm DH,Song JK

更新日期：2011-04-01 00:00:00

abstract：:Inhibitory compounds liberated from lignocellulose pretreatment are representative toxic chemicals that repress microbial growth and metabolism. A tolerant strain of the industrial yeast Saccharomyces cerevisiae is able to detoxify a major class of toxic compounds while producing ethanol. Knowledge on the yeast tolera...

journal_title：Applied microbiology and biotechnology

authors： Liu ZL,Huang X,Zhou Q,Xu J

更新日期：2019-07-01 00:00:00

abstract：:Glycerol is used by the cosmetic, paint, automotive, food, and pharmaceutical industries and for production of explosives. Currently, glycerol is available in commercial quantities as a by-product from biodiesel production, but the purity and the cost of its purification are prohibitive. The industrial production of g...

journal_title：Applied microbiology and biotechnology

authors： Semkiv MV,Dmytruk KV,Abbas CA,Sibirny AA

更新日期：2017-06-01 00:00:00

abstract：:Three bacterial strains utilizing paracetamol as the sole carbon, nitrogen, and energy source were isolated from a paracetamol-degrading aerobic aggregate, and assigned to species of the genera Stenotrophomonas and Pseudomonas. The Stenotrophomonas species have not included any known paracetamol degraders until now. I...

journal_title：Applied microbiology and biotechnology

authors： Zhang L,Hu J,Zhu R,Zhou Q,Chen J

更新日期：2013-04-01 00:00:00

abstract：:DNA polymerase I gene was cloned and sequenced from the thermophilic bacterium Geobacillus caldoxylosilyticus TK4. The gene is 2,634 bp long and encodes a protein of 878 amino acids in length. The enzyme has a molecular mass of 99 kDa and shows sequence homology with DNA polymerase I from Bacillus species (89% identit...

journal_title：Applied microbiology and biotechnology

authors： Sandalli C,Singh K,Modak MJ,Ketkar A,Canakci S,Demir I,Belduz AO

更新日期：2009-08-01 00:00:00

abstract：:The ability of acetic acid bacteria (AAB) to produce cellulose has gained much industrial interest due to the physical and chemical characteristics of bacterial cellulose. The production of cellulose occurs in the presence of oxygen and in a glucose-containing medium, but it can also occur during vinegar elaboration b...

journal_title：Applied microbiology and biotechnology

authors： Valera MJ,Torija MJ,Mas A,Mateo E

更新日期：2015-02-01 00:00:00

abstract：:There is a growing demand for silver-based biocides, including both ionic silver forms and metallic nanosilver. The use of metallic nanosilver, typically chemically produced, faces challenges including particle agglomeration, high costs, and upscaling difficulties . Additionally, there exists a need for the developmen...

journal_title：Applied microbiology and biotechnology

authors： Sintubin L,De Windt W,Dick J,Mast J,van der Ha D,Verstraete W,Boon N

更新日期：2009-09-01 00:00:00

abstract：:Molecular studies were performed to establish the causes of the superior lovastatin productivity of a novel solid-state fermentation (SSF) process, in relation with liquid submerged fermentation (SmF; 20 mg/g vs. 0.65 mg/ml). In SSF, biosynthetic genes lovE and lovF transcripts accumulated to high levels from day 1 to...

journal_title：Applied microbiology and biotechnology

authors： Barrios-González J,Baños JG,Covarrubias AA,Garay-Arroyo A

更新日期：2008-05-01 00:00:00

abstract：:Understanding of the effects of perturbation strategies on soil microorganisms is helpful in optimizing bioremediation systems and enhancing their efficiency. Four soil mesocosms were constructed for bioremediation of highly polycyclic aromatic hydrocarbon-contaminated soil using the flowering plant Orychophragmus vio...

journal_title：Applied microbiology and biotechnology

authors： Sun GD,Xu Y,Liu Y,Liu ZP

更新日期：2014-12-01 00:00:00

abstract：:The thermostable beta-glycosidase from Thermus thermophilus, cloned and overexpressed in Escherichia coli was used to catalyze the transfer of beta-D-glucosyl and beta-D-fucosyl from the corresponding p-nitrophenyl-beta-D-glycopyranosides to a hydroxyl group of glucose in the synthesis of beta-D-glucosyl-D-glucopyrano...

journal_title：Applied microbiology and biotechnology

authors： Fourage L,Colas B

更新日期：2001-08-01 00:00:00

abstract：:Protein titers, a key bioprocessing metric, depend both on the synthesis of protein and the degradation of protein. Secreted recombinant protein production in Saccharomyces cerevisiae is an attractive platform as minimal media can be used for cultivation, thus reducing fermentation costs and simplifying downstream pur...

journal_title：Applied microbiology and biotechnology

authors： Tyo KE,Liu Z,Magnusson Y,Petranovic D,Nielsen J

更新日期：2014-08-01 00:00:00

abstract：:The worldwide interest of the current era is to increase tendency towards the use of natural substances instead of synthetic ones. So, alternative and effective environment friendly sustainable technologies are highly needed. Due to a broad range of biological activities, fungi are considered as a significant source o...

journal_title：Applied microbiology and biotechnology

authors： Akilandeswari P,Pradeep BV

更新日期：2016-02-01 00:00:00