Exploration of industrially important pigments from soil fungi.

abstract：:Two bacterial strains, Ralstonia sp. LD35 and Pseudomonas putida DSM 1868, were assayed for their ability to degrade the monocyclic aromatic compounds commonly found in olive mill wastewaters (OMWs). The goal was to study the possibility of employing the two strains in the removal of these recalcitrant and toxic compo...

journal_title：Applied microbiology and biotechnology

authors： Di Gioia D,Fava F,Bertin L,Marchetti L

更新日期：2001-05-01 00:00:00

abstract：:Internal fragments of alpha- and beta-tubulin genes were generated using reverse transcription polymerase chain reaction (RT-PCR), and the termini were isolated using 5'- and 3'-rapid amplification of cDNA ends. Phytophthora capsici alpha- and beta-tubulin specific primers were then used to generate full-length cDNA b...

journal_title：Applied microbiology and biotechnology

authors： Koo BS,Park H,Kalme S,Park HY,Han JW,Yeo YS,Yoon SH,Kim SJ,Lee CM,Yoon MY

更新日期：2009-03-01 00:00:00

abstract：:Mutagenesis and selection were applied to a strain of Penicillium echinulatum by treating conidia with hydrogen peroxide or 1,2,7,8-diepoxyoctane and then by incubating the conidia for 48 h in broth containing microcrystalline cellulose washed in 0.5% (w/v) aqueous 2-deoxyglucose before plating them onto cellulose aga...

journal_title：Applied microbiology and biotechnology

authors： Dillon AJ,Zorgi C,Camassola M,Henriques JA

更新日期：2006-05-01 00:00:00

abstract：:Repetitious gene cassettes that encode the consensus decapeptide repeat of Mytilus edulis bioadhesive protein were designed, constructed, and expressed in Escherichia coli. The bioadhesive precursor (BP) with a relative molecular mass of 25,000 was expressed from one 600-bp gene at levels approaching 60% of total cell...

journal_title：Applied microbiology and biotechnology

authors： Salerno AJ,Goldberg I

更新日期：1993-05-01 00:00:00

abstract：:Two-stage technologies have been developed for anaerobic digestion of waste-activated sludge. In this study, the archaeal and bacterial community structure dynamics and bioprocess performance of a bench-scale two-stage anaerobic digester treating urban sewage sludge have been studied by the means of high-throughput se...

journal_title：Applied microbiology and biotechnology

authors： Gonzalez-Martinez A,Garcia-Ruiz MJ,Rodriguez-Sanchez A,Osorio F,Gonzalez-Lopez J

更新日期：2016-07-01 00:00:00

abstract：:Nanotechnology is a promising field of interdisciplinary research. It opens up a wide array of opportunities in various fields like medicine, pharmaceuticals, electronics and agriculture. The potential uses and benefits of nanotechnology are enormous. These include insect pests management through the formulations of n...

journal_title：Applied microbiology and biotechnology

authors： Rai M,Ingle A

更新日期：2012-04-01 00:00:00

abstract：:Physico-chemical parameters, hydrological conditions, and microbial interactions can affect the growth and persistence of cyanobacteria, but the interacting effects among these bloom-forming factors are still poorly known. This hampers our capacity to predict the occurrence of cyanobacterial bloom accurately. Here, we...

journal_title：Applied microbiology and biotechnology

authors： Song H,Xu J,Lavoie M,Fan X,Liu G,Sun L,Fu Z,Qian H

更新日期：2017-02-01 00:00:00

abstract：:Expression of recombinant proteins in Escherichia coli often requires use of inducible promoters to shorten the lag phase and improve protein productivity and final protein titer. Synthetic molecules that cannot be metabolized by E. coli, such as isopropyl thiogalactopyranoside (IPTG), have been frequently used to tri...

journal_title：Applied microbiology and biotechnology

authors： Anilionyte O,Liang H,Ma X,Yang L,Zhou K

更新日期：2018-08-01 00:00:00

abstract：:Anaerobic biodegradation of atrazine by the bacterial isolate M91-3 was characterized with respect to mineralization, metabolite formation, and denitrification. The ability of the isolate to enhance atrazine biodegradation in anaerobic sediment slurries was also investigated. The organism utilized atrazine as its sole...

journal_title：Applied microbiology and biotechnology

authors： Crawford JJ,Sims GK,Mulvaney RL,Radosevich M

更新日期：1998-05-01 00:00:00

abstract：:Under alternating aerobic/anaerobic conditions and without additional carbon sources, a bacterial consortium consisting initially of 18 bacterial strains was obtained in a sequence batch reactor. The phosphorus removal capability could only be maintained using sterile filtrate of activated sludge as medium. The additi...

journal_title：Applied microbiology and biotechnology

authors： Hollender J,Dreyer U,Kornberger L,Kämpfer P,Dott W

更新日期：2002-01-01 00:00:00

abstract：:Prenyltransferase NovQ is a vital class involved in the biosynthesis of secondary metabolites such as clorobiocin and novobiocin. To investigate the relationship between structure and catalytic properties of NovQ, here, we have analyzed the substrate-binding site, namely PT barrel, and revealed that menadione hydroqui...

journal_title：Applied microbiology and biotechnology

authors： Ni W,Zheng Z,Liu H,Wang P,Wang H,Sun X,Yang Q,Fang Z,Tang H,Zhao G

更新日期：2020-05-01 00:00:00

abstract：:Denitrifying sulfide removal (DSR) process simultaneously converts sulfide, nitrate, and chemical oxygen demand from industrial wastewaters to elemental sulfur, nitrogen gas, and carbon dioxide, respectively. This investigation utilizes a dilution-to-extinction approach at 10(-2) to 10(-6) dilutions to elucidate the c...

journal_title：Applied microbiology and biotechnology

authors： Chen C,Ren N,Wang A,Liu L,Lee DJ

更新日期：2010-03-01 00:00:00

abstract：:Removal of tetrachloroethylene (perchloroethylene; C2Cl4) by microbial consortia from two sites with different C2Cl4 exposure histories was examined in a bench-scale anaerobic column bioreactor. It was hypothesized that optimal removal would be observed in the reactor packed with sediments having an extensive exposure...

journal_title：Applied microbiology and biotechnology

authors： Noftsker C,Watwood ME

更新日期：1997-09-01 00:00:00

abstract：:Aspergillus oryzae is a fungus used extensively in the fermentation industry. We constructed cDNA microarrays comprising 2,070 highly expressed cDNAs selected from the approximately 6,000 non-redundant expressed sequence tags (ESTs) in the A. oryzae EST database (http://www.aist.go.jp/RIODB/ffdb/index.html). Using the...

journal_title：Applied microbiology and biotechnology

authors： Maeda H,Sano M,Maruyama Y,Tanno T,Akao T,Totsuka Y,Endo M,Sakurada R,Yamagata Y,Machida M,Akita O,Hasegawa F,Abe K,Gomi K,Nakajima T,Iguchi Y

更新日期：2004-07-01 00:00:00

abstract：:The aim of this study was to determine the antimicrobial effects of lemongrass essential oil (C. flexuosus) and to determine cytotoxic effects of both test compounds on human dermal fibroblasts. Antimicrobial susceptibility screening was carried out using the disk diffusion method. Antimicrobial resistance was observe...

journal_title：Applied microbiology and biotechnology

authors： Adukwu EC,Bowles M,Edwards-Jones V,Bone H

更新日期：2016-11-01 00:00:00

abstract：:Sialylation affects circulating half-life, charge distribution, and other biochemical properties of therapeutic glycoproteins. Loss of protein sialylation during glycoprotein-producing bioprocesses could lead to a low final protein sialylation level and bring negative effects on subsequent clinical efficacy. In this w...

journal_title：Applied microbiology and biotechnology

authors： Chen X,Liu X,Xiao Z,Liu J,Zhao L,Tan WS,Fan L

更新日期：2019-06-01 00:00:00

abstract：:Two cephalosporin genes from Acremonium chrysogenum, pcbAB and pcbC encode the ACV (alpha-aminoadipyl-cysteinyl-valine) synthetase and isopenicillin N-synthetase, respectively. The two adjacent genes are orientated in opposite directions on the chromosomal DNA, separated by a 1.2-kb non-translated sequence, carrying t...

journal_title：Applied microbiology and biotechnology

authors： Menne S,Walz M,Kück U

更新日期：1994-10-01 00:00:00

abstract：:Enterokinase is one of the most frequently used enzymes for the removal of affinity tags from target recombinant proteins. In this study, several fermentation strategies were assayed for the production of human enterokinase in Pichia pastoris under constitutive GAP promoter. Two of them with controlled specific growth...

journal_title：Applied microbiology and biotechnology

authors： Melicherová K,Krahulec J,Šafránek M,Lišková V,Hopková D,Széliová D,Turňa J

更新日期：2017-03-01 00:00:00

abstract：:Various single-cell isolation techniques, including dilution, micromanipulation, flow cytometry, microfluidics, and compartmentalization, have been developed. These techniques can be used to cultivate previously uncultured microbes, to assess and monitor cell physiology and function, and to screen for novel microbiolo...

journal_title：Applied microbiology and biotechnology

authors： Ishii S,Tago K,Senoo K

更新日期：2010-05-01 00:00:00

abstract：:Pyruvate carboxylase is an important anaplerotic enzyme replenishing oxaloacetate consumed for biosynthesis during growth, or lysine and glutamic acid production in industrial fermentations. We used regions of homology from pyruvate carboxylase sequences of 12 different species (corresponding to the ATP- and pyruvate-...

journal_title：Applied microbiology and biotechnology

authors： Koffas MA,Ramamoorthi R,Pine WA,Sinskey AJ,Stephanopoulos G

更新日期：1998-09-01 00:00:00

abstract：:Indigo is an insoluble blue dye historically used for dyeing textiles. A traditional approach for indigo dyeing involves microbial reduction of polygonum indigo to solubilize it under alkaline conditions; however, the mechanism by which microorganisms reduce indigo remains poorly understood. Here, we aimed to identify...

journal_title：Applied microbiology and biotechnology

authors： Suzuki H,Abe T,Doi K,Ohshima T

更新日期：2018-11-01 00:00:00

abstract：:The rapid global urbanization and other extensive anthropogenic activities exacerbated the worldwide human health risks induced by antibiotic resistance genes (ARGs). Knowledge of the origins and dissemination of ARGs is essential for understanding modern resistome, while little information is known regarding the over...

journal_title：Applied microbiology and biotechnology

authors： Ouyang WY,Huang FY,Zhao Y,Li H,Su JQ

更新日期：2015-07-01 00:00:00

abstract：:Since 2015, outbreaks of hepatitis-hydropericardium syndrome (HPS) caused by a novel genotype of fowl adenovirus 4 (FAdV-4) infection have created serious economic losses in China. Given that other serotypes of hypervirulent FAdVs have also been reported in poultry around the world, a common ELISA for all serotypes wi...

journal_title：Applied microbiology and biotechnology

authors： Pan Q,Wang J,Gao Y,Cui H,Liu C,Qi X,Zhang Y,Wang Y,Li K,Gao L,Wang X

更新日期：2020-01-01 00:00:00

abstract：:A set of filamentous fungi (42 strains) was screened for alpha-N-acetylgalactosaminidase activity, and a series of inducers and different cultivation conditions were tested. Enzyme production by the best producer Aspergillus niger CCIM K2 was optimized and scaled up. alpha-N-Acetylgalactosaminidase was purified to app...

journal_title：Applied microbiology and biotechnology

authors： Weignerová L,Filipi T,Manglová D,Kren V

更新日期：2008-07-01 00:00:00

abstract：:AmyC, a glycoside hydrolase family 57 (GH57) enzyme of Thermotoga maritima MSB8, has previously been identified as an intracellular α-amylase playing a role in either maltodextrin utilization or storage polysaccharide metabolism. However, the α-amylase specificity of AmyC is questionable as extensive phylogenetic anal...

journal_title：Applied microbiology and biotechnology

authors： Zhang X,Leemhuis H,Janeček Š,Martinovičová M,Pijning T,van der Maarel MJEC

更新日期：2019-08-01 00:00:00

abstract：:The human cell line rF2N78 produces an antibody with a high galactosylation ratio which resembles human IgG. However, it has been observed that the aglycosylated antibody starts to appear when glucose is depleted. To determine whether glucose depletion is a main cause for aglycosylation of the antibody, fed-batch cult...

journal_title：Applied microbiology and biotechnology

authors： Seo JS,Min BS,Kim YJ,Cho JM,Baek E,Cho MS,Lee GM

更新日期：2014-04-01 00:00:00

abstract：:The lack of a universal method to extract RNA from soil hinders the progress of studies related to nitrification in soil, which is an important step in the nitrogen cycle. It is particularly difficult to extract RNA from certain types of soils such as Andosols (volcanic ash soils), which is the dominant agricultural s...

journal_title：Applied microbiology and biotechnology

authors： Wang Y,Nagaoka K,Hayatsu M,Sakai Y,Tago K,Asakawa S,Fujii T

更新日期：2012-11-01 00:00:00

abstract：:A cost-effective conversion of lignocellulosic biomass into bioethanol requires that the xylose released from the hemicellulose fraction (20-40% of biomass) can be fermented. Baker's yeast, Saccharomyces cerevisiae, efficiently ferments glucose but it lacks the ability to ferment xylose. Xylose-fermenting yeast such a...

journal_title：Applied microbiology and biotechnology

authors： Hou X

更新日期：2012-04-01 00:00:00

abstract：:The goal of modern taxonomy is to understand the relationships of living organisms in terms of evolutionary descent. Thereby, the relationships between living organisms are understood in terms of nested clades--every time a speciation event takes place, two new clades are produced. Life comprises three domains of livi...

journal_title：Applied microbiology and biotechnology

authors： Voigt K,Kirk PM

更新日期：2011-04-01 00:00:00

abstract：:An immobilized-cell biofilm reactor was used for the continuous production of lactic acid by Lactobacillus casei subsp. rhamnosus (ATCC 11443). At Iowa State University, a unique plastic composite support (PCS) that stimulates biofilm formation has been developed. The optimized PCS blend for Lactobacillus contains 50%...

journal_title：Applied microbiology and biotechnology

authors： Cotton JC,Pometto AL 3rd,Gvozdenovic-Jeremic J

更新日期：2001-12-01 00:00:00