Functional consortium for denitrifying sulfide removal process.

abstract：:The L-rhamnose isomerase gene (L-rhi) encoding for L-rhamnose isomerase (L-RhI) from Bacillus pallidus Y25, a facultative thermophilic bacterium, was cloned and overexpressed in Escherichia coli with a cooperation of the 6xHis sequence at a C-terminal of the protein. The open reading frame of L-rhi consisted of 1,236 ...

journal_title：Applied microbiology and biotechnology

authors： Poonperm W,Takata G,Okada H,Morimoto K,Granström TB,Izumori K

更新日期：2007-10-01 00:00:00

abstract：:Biosynthesis of Ds(+)-threo-isocitric acid from ethanol in the Yarrowia lipolytica batch and repeated-batch cultures was studied. Repeated-batch cultivation was found to provide for a good biosynthetic efficiency of the producer for as long as 748 h, probably due to maintenance of high activities of enzymes involved i...

journal_title：Applied microbiology and biotechnology

authors： Morgunov IG,Kamzolova SV,Karpukhina OV,Bokieva SB,Inozemtsev AN

更新日期：2019-04-01 00:00:00

abstract：:Bacterial cells are protected by an exoskeleton, the stabilizing and shape-maintaining cell wall, consisting of the complex macromolecule peptidoglycan. In view of its function, it could be assumed that the cell wall is a static structure. In truth, however, it is steadily broken down by peptidoglycan-cleaving enzymes...

journal_title：Applied microbiology and biotechnology

authors： Reith J,Mayer C

更新日期：2011-10-01 00:00:00

abstract：:MeBglD2, a β-glycosidase that is highly activated in the presence of various monosaccharides and disaccharides, was isolated from a soil metagenomic library. MeBglD2 had not only β-glucosidase activity but also β-galactosidase and β-fucosidase activities. MeBglD2 β-glucosidase activity increased in a cellobiose concen...

journal_title：Applied microbiology and biotechnology

authors： Matsuzawa T,Yaoi K

更新日期：2017-01-01 00:00:00

abstract：:The integration of physiological knowledge into process control strategies is a cornerstone for the improvement of biopharmaceutical cell culture technologies. The present contribution investigates the applicability of specific productivity as a physiological control parameter in a cell culture process producing a mon...

journal_title：Applied microbiology and biotechnology

authors： Zalai D,Hevér H,Lovász K,Molnár D,Wechselberger P,Hofer A,Párta L,Putics Á,Herwig C

更新日期：2016-08-01 00:00:00

abstract：:Chemical mutagenesis of the phototrophic green microalga Parietochloris incisa, producing high amounts of arachidonic acid (ARA), resulted in selection of a mutant deficient in ARA and rich in dihomo-γ-linolenic acid (DGLA) and thus ∆5 desaturase defective. The mutagenesis produced a nonsense mutation in the ∆5 desatu...

journal_title：Applied microbiology and biotechnology

authors： Iskandarov U,Khozin-Goldberg I,Cohen Z

更新日期：2011-04-01 00:00:00

abstract：:The anaerobic degradation of phenol was studied in a fed-batch culture. Nitrate was added as electron acceptor and phenol was provided three times, to a final concentration of 200 mg/l. Randomly amplified polymorphic DNA (RAPD) and terminal fraction fragment length polymorphism (T-RFLP) were used and compared in order...

journal_title：Applied microbiology and biotechnology

authors： Guieysse B,Wickström P,Forsman M,Mattiasson B

更新日期：2001-09-01 00:00:00

abstract：:In this study, the production of enantiomerically pure (1R,4S,6S)-6-hydroxy-bicyclo[2.2.2]octane-2-one ((-)-2) through stereoselective bioreduction was used as a model reaction for the comparison of engineered Saccharomyces cerevisiae and engineered Escherichia coli as biocatalysts. For both microorganisms, over-expre...

journal_title：Applied microbiology and biotechnology

authors： Skorupa Parachin N,Carlquist M,Gorwa-Grauslund MF

更新日期：2009-09-01 00:00:00

abstract：:The microbial production of biofuels is a promising avenue for the development of viable processes for the generation of fuels from sustainable resources. In order to become cost and energy effective, these processes must utilize organisms that can be optimized to efficiently produce candidate fuels from a variety of ...

journal_title：Applied microbiology and biotechnology

authors： Clomburg JM,Gonzalez R

更新日期：2010-03-01 00:00:00

abstract：:ω-Transaminase (ω-TA) is an attractive alternative to metal catalysts for the stereoselective amination of prochiral ketones. The narrow substrate scope of an R-ω-transaminase from Mycobacterium vanbaalenii (MvTA) limits its application in R-amine synthesis. A fluorescence-based TA activity screening system was develo...

journal_title：Applied microbiology and biotechnology

authors： Cheng F,Chen XL,Xiang C,Liu ZQ,Wang YJ,Zheng YG

更新日期：2020-04-01 00:00:00

abstract：:This study reports enzymatic hydrolysis of the biomass of the giant reed (Arundo donax L.) after ammonia fibre expansion (AFEX) pretreatment. In particular, the capacity of the arabinofuranosidase from the fungus Pleurotus ostreatus recombinantly expressed in Pichia pastoris rPoAbf, its evolved mutant rPoAbf F435Y/Y44...

journal_title：Applied microbiology and biotechnology

authors： Giacobbe S,Balan V,Montella S,Fagnano M,Mori M,Faraco V

更新日期：2016-03-01 00:00:00

abstract：:Two lab-scale plug flow activated sludge reactors were run in parallel for 4 months at 30 and 55 degrees C. Research focussed on: (1) COD (chemical oxygen demand) removal, (2) effluent turbidity at both temperatures, (3) the origin of effluent colloidal material and (4) the possible role of protozoa on turbidity level...

journal_title：Applied microbiology and biotechnology

authors： Vogelaar JC,van Lier JB,Klapwijk B,de Vries MC,Lettinga G

更新日期：2002-06-01 00:00:00

abstract：:The present study aimed to evaluate the in vitro antioxidant activities and the protective effect of Rhodobacter sphaeroides on H2O2-induced oxidative stress in Caco-2 cells. The results showed that the antioxidant action of R. sphaeroides varied with different cell concentrations and treatments. Also, the intact cell...

journal_title：Applied microbiology and biotechnology

authors： An J,Yang C,Li Z,Finn PW,Perkins DL,Sun J,Bai Z,Gao L,Zhang M,Ren D

更新日期：2019-01-01 00:00:00

abstract：:Streptococcus thermophilus (ST) is a lactic acid bacterium widely used in dairy industry and displays several properties which could be beneficial for host. The objective of this study was to investigate, in vitro, the implication of sortase A (SrtA) and sortase-dependent proteins (SDPs) in the adhesion of ST LMD-9 st...

journal_title：Applied microbiology and biotechnology

authors： Kebouchi M,Galia W,Genay M,Soligot C,Lecomte X,Awussi AA,Perrin C,Roux E,Dary-Mourot A,Le Roux Y

更新日期：2016-04-01 00:00:00

abstract：:Trichothecenes are toxic secondary metabolites produced by filamentous fungi mainly belonging to the Fusarium genus. Production of these mycotoxins occurs during infection of crops and is a threat to human and animal health. Although the pathway for biosynthesis of trichothecenes is well established, the regulation of...

journal_title：Applied microbiology and biotechnology

authors： Merhej J,Richard-Forget F,Barreau C

更新日期：2011-08-01 00:00:00

abstract：:The polyene macrolide rimocidin, produced by Streptomyces rimosus M527, is highly effective against a broad range of fungal plant pathogens, but at low yields. Elicitation is an effective method of stimulating the yield of bioactive secondary metabolites. In this study, the biomass and filtrate of a culture broth of E...

journal_title：Applied microbiology and biotechnology

authors： Song Z,Ma Z,Bechthold A,Yu X

更新日期：2020-05-01 00:00:00

abstract：:Two sequencing batch reactors were synchronously operated to investigate the effect of manganese (II) (Mn(2+)) augmentation on aerobic granulation. Reactor 1 (R1) was added with 10 mg/L Mn(2+), while there was no Mn(2+) augmentation in reactor 2 (R2). Results showed that R1 had a faster granulation process than R2 and...

journal_title：Applied microbiology and biotechnology

authors： Huang L,Yang T,Wang W,Zhang B,Sun Y

更新日期：2012-03-01 00:00:00

abstract：:Paddy fields are a significant source of methane and contribute up to 20% of total methane emissions from wetland ecosystems. These inundated, anoxic soils featuring abundant nitrogen compounds and methane are an ideal niche for nitrate-dependent anaerobic methanotrophs. After 2 years of enrichment with a continuous s...

journal_title：Applied microbiology and biotechnology

authors： Vaksmaa A,Guerrero-Cruz S,van Alen TA,Cremers G,Ettwig KF,Lüke C,Jetten MSM

更新日期：2017-09-01 00:00:00

abstract：:The genetic diversity and expression of amoA of autotrophic ammonia oxidizers in wastewater treatment processes were investigated by RT-PCR and denaturing gradient gel electrophoresis (DGGE) in order to identify active components of ammonia-oxidizer populations in a such processes. Ammonia oxidizers, evidenced by the ...

journal_title：Applied microbiology and biotechnology

authors： Ebie Y,Noda N,Miura H,Matsumura M,Tsuneda S,Hirata A,Inamori Y

更新日期：2004-06-01 00:00:00

abstract：:In industry, filamentous fungi have a prominent position as producers of economically relevant primary or secondary metabolites. Particularly, the advent of genetic engineering of filamentous fungi has led to a growing number of molecular tools to adopt filamentous fungi for biotechnical applications. Here, we summari...

journal_title：Applied microbiology and biotechnology

authors： Kluge J,Terfehr D,Kück U

更新日期：2018-08-01 00:00:00

abstract：:Transformants of the Trichoderma reeseistrains QM9414 and Rut-C30 were constructed in which the genes for the two major hydrophobin proteins, hydrophobins I (HFBI) and II (HFBII), were deleted or amplified by molecular biological techniques. Growth parameters and foam production of the transformant strains were compar...

journal_title：Applied microbiology and biotechnology

authors： Bailey MJ,Askolin S,Hörhammer N,Tenkanen M,Linder M,Penttilä M,Nakari-Setälä T

更新日期：2002-05-01 00:00:00

abstract：:Escherichia coli normally cannot utilize the β-glucoside sugar cellobiose as a carbon and energy source unless a stringent selection pressure for survival is present. The cellobiose-utilization phenotype can be conferred by mutations in the two cryptic operons, chb and asc. In this study, the cellobiose-utilization ph...

journal_title：Applied microbiology and biotechnology

authors： Vinuselvi P,Lee SK

更新日期：2011-10-01 00:00:00

abstract：:To meet the demand for sustainable energy, aquifer thermal energy storage (ATES) is widely used in the subsurface in urban areas. However, contamination of groundwater, especially with chlorinated volatile organic compounds (CVOCs), is often being encountered. This is commonly seen as an impediment to ATES implementat...

journal_title：Applied microbiology and biotechnology

authors： Ni Z,van Gaans P,Smit M,Rijnaarts H,Grotenhuis T

更新日期：2016-04-01 00:00:00

abstract：:The genome of the coprophilous fungus Podospora anserina displays an impressive array of genes encoding hemicellulolytic enzymes. In this study, we focused on a putative carbohydrate esterase (CE) from family 16 (CE16) that bears a carbohydrate-binding module from family CBM1. The protein was heterologously expressed ...

journal_title：Applied microbiology and biotechnology

authors： Puchart V,Berrin JG,Haon M,Biely P

更新日期：2015-12-01 00:00:00

abstract：:Dengue virus (DENV) infection is one of the most important public health threats globally; however, no vaccines or effective antivirals are currently available. The bioluminescence imaging technique has emerged as a powerful tool for studies on viral pathogenesis in vitro and in vivo. In this study, using a recombinan...

journal_title：Applied microbiology and biotechnology

authors： Li XF,Deng YQ,Zhao H,Ye Q,Wang HJ,Li SH,Zhu SY,Shi PY,Qin ED,Zhang B,Qin CF

更新日期：2013-05-01 00:00:00

abstract：:The recent, rapid increase in bacterial antimicrobial resistance has become a major public health concern. One approach to generate new classes of antibacterials is targeting virulence rather than the viability of bacteria. Proteins of the Dsb system, which play a key role in the virulence of many pathogenic microorga...

journal_title：Applied microbiology and biotechnology

authors： Bocian-Ostrzycka KM,Grzeszczuk MJ,Banaś AM,Jagusztyn-Krynicka EK

更新日期：2017-05-01 00:00:00

abstract：:The gene from Xanthomonas campestris pv. phaseoli that is involved in the C5 pathway of delta-amino-levulinic acid (ALA) of Escherichia coli. Subcloning of deletion fragments from the initial 2.5-kilobase (kb) chromosomal fragment allowed the isolation of a 1.6-kb fragment that could complement the hemM mutation. Nucl...

journal_title：Applied microbiology and biotechnology

authors： Asahara N,Murakami K,Korbrisate S,Hashimoto Y,Murooka Y

更新日期：1994-02-01 00:00:00

abstract：:Knowledge about methanotrophs and their activities is important to understand the microbial mediation of the greenhouse gas CH(4) under climate change and human activities in terrestrial ecosystems. The effects of simulated warming and sheep grazing on methanotrophic abundance, community composition, and activity were...

journal_title：Applied microbiology and biotechnology

authors： Zheng Y,Yang W,Sun X,Wang SP,Rui YC,Luo CY,Guo LD

更新日期：2012-03-01 00:00:00

abstract：:A precise and scarless genome excision method, employing the Cre/loxP system in concert with double-strand break (DSB)-stimulated intramolecular recombination was developed. The DSBs were mediated by the restriction endonuclease, I-SceI. It permitted multiple deletions of independent 14-, 43-, and 10-kb-long genomic r...

journal_title：Applied microbiology and biotechnology

authors： Suzuki N,Nonaka H,Tsuge Y,Okayama S,Inui M,Yukawa H

更新日期：2005-11-01 00:00:00

abstract：:β-Glucosidases designated MoCel3A and MoCel3B were successfully overexpressed in Magnaporthe oryzae. MoCel3A and MoCel3B showed optimal activity at 50 °C and pH 5.0-5.5. MoCel3A exhibited higher activity on higher degree of polymerization (DP) oligosaccharides and on β-1,3-linked oligosaccharides than on β-1,4-linked ...

journal_title：Applied microbiology and biotechnology

authors： Takahashi M,Konishi T,Takeda T

更新日期：2011-08-01 00:00:00