Continuous lactic acid fermentation using a plastic composite support biofilm reactor.

abstract：:Dibenzocyclooctadiene lignans are a specific group of secondary metabolites that occur solely in Schisandra chinensis. The aim of the presented work was to boost the accumulation of lignans in the agitated microshoot cultures of S. chinensis, using different elicitation schemes. The experiments included testing of var...

journal_title：Applied microbiology and biotechnology

authors： Szopa A,Kokotkiewicz A,Król A,Luczkiewicz M,Ekiert H

更新日期：2018-01-01 00:00:00

abstract：:Anode properties are critical for the performance of microbial electrolysis cells (MECs). In the present study, Fe nanoparticle-modified graphite disks were used as anodes to investigate the effects of nanoparticles on the performance of Shewanella oneidensis MR-1 in MECs. Results demonstrated that the average current...

journal_title：Applied microbiology and biotechnology

authors： Xu S,Liu H,Fan Y,Schaller R,Jiao J,Chaplen F

更新日期：2012-01-01 00:00:00

abstract：:In response to changing extracellular pH levels, phosphate-limited continuous cultures of Clostridium acetobutylicum reversibly switches its metabolism from the dominant formation of acids to the prevalent production of solvents. Previous experimental and theoretical studies have revealed that this pH-induced metaboli...

journal_title：Applied microbiology and biotechnology

authors： Millat T,Janssen H,Thorn GJ,King JR,Bahl H,Fischer RJ,Wolkenhauer O

更新日期：2013-07-01 00:00:00

abstract：:Two recombinant Aeropyrum pernix glutamate dehydrogenase (GDH) enzymes with different length N-termini were cloned and expressed in Escherichia coli: sGDH begins with the amino acid sequence of the extracted native enzyme (M-Q-P-T-D-P-L-E-E), whereas lGDH begins with the sequence of the predicted ORF (M-E-V-L-A-L-Q-P-...

journal_title：Applied microbiology and biotechnology

authors： Helianti I,Morita Y,Murakami Y,Yokoyama K,Tamiya E

更新日期：2002-08-01 00:00:00

abstract：:Enzymes are natural catalysts highly specific to the substrate type and operate under mild conditions of temperature, pressure, and pH with high conversion rates, which makes them more efficient than conventional chemical catalysts. The enzymes can be obtained from various sources, animal, vegetable, and microbiologic...

journal_title：Applied microbiology and biotechnology

authors： Filho DG,Silva AG,Guidini CZ

更新日期：2019-09-01 00:00:00

abstract：:The development of cell printing is vital for establishing biofabrication approaches as clinically relevant tools. Achieving this requires bio-inks which must not only be easily printable, but also allow controllable and reproducible printing of cells. This review outlines the general principles and current progress a...

journal_title：Applied microbiology and biotechnology

authors： Ferris CJ,Gilmore KG,Wallace GG,In het Panhuis M

更新日期：2013-05-01 00:00:00

abstract：:Rhizopus oryzae is a filamentous fungus belonging to the Zygomycetes. It is among others known for its ability to produce the sustainable platform chemicals L: -(+)-lactic acid, fumaric acid, and ethanol. During glycolysis, all fermentable carbon sources are metabolized to pyruvate and subsequently distributed over th...

journal_title：Applied microbiology and biotechnology

authors： Meussen BJ,de Graaff LH,Sanders JP,Weusthuis RA

更新日期：2012-05-01 00:00:00

abstract：:Demand for novel antifungal drugs for medical and agricultural uses has been increasing because of the diversity of pathogenic fungi and the emergence of drug-resistant strains. Genomic resources for various living species, including pathogenic fungi, can be utilized to develop novel and effective antifungal compounds...

journal_title：Applied microbiology and biotechnology

authors： Marui J,Yoshimi A,Hagiwara D,Fujii-Watanabe Y,Oda K,Koike H,Tamano K,Ishii T,Sano M,Machida M,Abe K

更新日期：2010-08-01 00:00:00

abstract：:The chemoenzymatic process involving biocatalytic resolution of rac-2-carboxyethyl-3-cyano-5-methylhexanoic acid ethyl ester (CNDE, 1) has been the most competitive and attractive route for pregabalin. A new esterase-producing strain ZJB-09203, which exhibited high hydrolytic activity, excellent enantioselectivity, an...

journal_title：Applied microbiology and biotechnology

authors： Zheng RC,Wang TZ,Fu DJ,Li AP,Li XJ,Zheng YG

更新日期：2013-06-01 00:00:00

abstract：:Superoxide dismutase (SOD) activity is one major defense line against oxidative stress for all of the aerobic organisms, and industrial production of this enzyme is highly demanded. The Cu/Zn superoxide dismutase gene (KmSOD1) of Kluyveromyces marxianus L3 was cloned and characterized. The deduced KmSod1p protein shar...

journal_title：Applied microbiology and biotechnology

authors： Raimondi S,Uccelletti D,Matteuzzi D,Pagnoni UM,Rossi M,Palleschi C

更新日期：2008-01-01 00:00:00

abstract：:Under alternating aerobic/anaerobic conditions and without additional carbon sources, a bacterial consortium consisting initially of 18 bacterial strains was obtained in a sequence batch reactor. The phosphorus removal capability could only be maintained using sterile filtrate of activated sludge as medium. The additi...

journal_title：Applied microbiology and biotechnology

authors： Hollender J,Dreyer U,Kornberger L,Kämpfer P,Dott W

更新日期：2002-01-01 00:00:00

abstract：:A precise and scarless genome excision method, employing the Cre/loxP system in concert with double-strand break (DSB)-stimulated intramolecular recombination was developed. The DSBs were mediated by the restriction endonuclease, I-SceI. It permitted multiple deletions of independent 14-, 43-, and 10-kb-long genomic r...

journal_title：Applied microbiology and biotechnology

authors： Suzuki N,Nonaka H,Tsuge Y,Okayama S,Inui M,Yukawa H

更新日期：2005-11-01 00:00:00

abstract：:Pseudomonas resinovorans, a known medium-chain-length (mcl-) poly(hydroxyalkanoate) (PHA) producer, was grown on 13-methyltetradecanoic acid (13-MTDA) and a mixture of isostearic acid (IA) isomers to produce methyl-branched mcl-PHA polymers. Shake-flask experiments revealed polymer productivities (the percent of the c...

journal_title：Applied microbiology and biotechnology

authors： Ashby RD,Ngo HL,Solaiman DK,Strahan G

更新日期：2009-11-01 00:00:00

abstract：:Geminiviruses have evolved with tremendous potential of recombination and possess the ability to manipulate several cellular processes of hosts. Chilli leaf curl virus (ChiLCV) is a monopartite Begomovirus (family Geminiviridae) which has emerged as a serious threat to chilli production worldwide. To date, development...

journal_title：Applied microbiology and biotechnology

authors： Kushwaha N,Sahu PP,Prasad M,Chakraborty S

更新日期：2015-06-01 00:00:00

abstract：:The paper describes the preparation of new probiotic formulations based on chitosan-coated alginate microcapsules containing three different probiotic strains, Lactobacillus plantarum PBS067, Lactobacillus rhamnosus PBS070, and Bifidobacterium animalis subsp. lactis PBS075 taken individually and as a mixture of them. ...

journal_title：Applied microbiology and biotechnology

authors： D'Orazio G,Di Gennaro P,Boccarusso M,Presti I,Bizzaro G,Giardina S,Michelotti A,Labra M,La Ferla B

更新日期：2015-11-01 00:00:00

abstract：:Two putative chitinase genes, lin0153 and lin1996, from the nonpathogenic bacterium Listeria innocua were expressed in Escherichia coli, and the gene products were characterized. The genes were close homologs of chitinases from the pathogenic bacterium Listeria monocytogenes, in which chitinases and chitin-binding pro...

journal_title：Applied microbiology and biotechnology

authors： Honda S,Wakita S,Sugahara Y,Kawakita M,Oyama F,Sakaguchi M

更新日期：2016-09-01 00:00:00

abstract：:A microbial peptide amidase was found in a limited screening and purified about 500-fold from Stenotrophomonas maltophilia. The native enzyme has a molecular mass of 38 kDa (gel filtration). The sequence of the first 16 amino acids was determined by Edman degradation. The isoelectric point was found to be around 5.8. ...

journal_title：Applied microbiology and biotechnology

authors： Stelkes-Ritter U,Wyzgol K,Kula MR

更新日期：1995-12-01 00:00:00

abstract：:Polyunsaturated fatty acids, docosahexaenoic acid (DHA, 22:6, n-3), eicosapentaenoic acid (EPA, 20:5, n-3), and arachidonic acid (ARA, 20:4 n-6), have multiple beneficial effects on human health and can be used as an important ingredient in dietary supplements, food, feed and pharmaceuticals. A variety of microorganis...

journal_title：Applied microbiology and biotechnology

authors： Wu L,Roe CL,Wen Z

更新日期：2013-09-01 00:00:00

abstract：:Yarrowia lipolytica is a promising platform for single cell oil production. It is well-known for its metabolism oriented toward utilization of hydrophobic substrates and accumulation of storage lipids. Multiple copies of DGA2 under constitutive promoter were introduced into the Q4 strain, a quadruple mutant deleted fo...

journal_title：Applied microbiology and biotechnology

authors： Gajdoš P,Nicaud JM,Rossignol T,Čertík M

更新日期：2015-10-01 00:00:00

abstract：:Poly(hydroxyalkanoates) (PHAs) constitute biodegradable polyesters and are considered among the most promising candidates to replace common petrochemical plastics in various applications. To date, all commercial processes for PHA production employ microbial discontinuous fed-batch fermentations. These processes featur...

journal_title：Applied microbiology and biotechnology

authors： Atlić A,Koller M,Scherzer D,Kutschera C,Grillo-Fernandes E,Horvat P,Chiellini E,Braunegg G

更新日期：2011-07-01 00:00:00

abstract：:Phenylacetaldehyde reductase (PAR) from Rhodococcus sp. ST-10 is useful for chiral alcohol production because of its broad substrate specificity and high stereoselectivity. The conversion of ketones into alcohols by PAR requires the coenzyme NADH. PAR can regenerate NADH by oxidizing additional alcohols, especially 2-...

journal_title：Applied microbiology and biotechnology

authors： Makino Y,Dairi T,Itoh N

更新日期：2007-12-01 00:00:00

abstract：:Functional screening for lipolytic enzymes at low temperatures resulted in the isolation of the novel cold-active esterases, EstM-N1 and EstM-N2, from a metagenomic DNA library of arctic soil samples. EstM-N1 and EstM-N2 were 395 and 407 amino acids in length, respectively, and showed the highest similarity to class C...

journal_title：Applied microbiology and biotechnology

authors： Yu EY,Kwon MA,Lee M,Oh JY,Choi JE,Lee JY,Song BK,Hahm DH,Song JK

更新日期：2011-04-01 00:00:00

abstract：:1,3-Propanediol (1,3-PD) production by fermentation of glycerol was described in 1881 but little attention was paid to this microbial route for over a century. Glycerol conversion to 1,3-PD can be carried out by Clostridia as well as Enterobacteriaceae. The main intermediate of the oxidative pathway is pyruvate, the f...

journal_title：Applied microbiology and biotechnology

authors： Biebl H,Menzel K,Zeng AP,Deckwer WD

更新日期：1999-09-01 00:00:00

abstract：:Using organically synthesized hydroxyalkanoate coenzyme A thioesters, the activities of two short-chain polyhydroxalkanoate (PHA) synthases were investigated--Ralstonia eutropha PHA synthase (a type I PHA synthase) and Ectothiorhodospira shaposhnikovii PHA synthase (a type III synthase). The results indicate that the ...

journal_title：Applied microbiology and biotechnology

authors： Zhang S,Kamachi M,Takagi Y,Lenz RW,Goodwin S

更新日期：2001-07-01 00:00:00

abstract：:This study aimed to model the inactivation of Lactobacillus brevis DSM 6235 while retaining the viability of yeasts during washing brewer's yeast with phosphoric acid and chlorine dioxide. The independent variables in the acid washing were pH (1-3) and temperature (1-9 °C), whereas in the washing with chlorine dioxide...

journal_title：Applied microbiology and biotechnology

authors： Munford ARG,Chaves RD,Granato D,Sant'Ana AS

更新日期：2020-05-01 00:00:00

abstract：:Using a randomly-cloned, HindIII-digested, chromosomal fragment from Lactococcus lactis subsp. lactis LM0230, first-generation lactococcal integrative cloning vectors were developed. Through dideoxy DNA sequence analysis, the cloned chromosomal DNA fragment was determined to be 1026 base pairs. Southern hybridization ...

journal_title：Applied microbiology and biotechnology

authors： McIntyre DA,Harlander SK

更新日期：1993-11-01 00:00:00

abstract：:We have constructed a chimeric protein composed of the receptor binding and membrane translocation domains of Pseudomonas exotoxin A (PE) with the outer membrane proteins I and F, together designated as PEIF. The potential of PEIF as a vaccine against Pseudomonas infection was evaluated in BALB/c mice and New Zealand ...

journal_title：Applied microbiology and biotechnology

authors： Chen TY,Shang HF,Chen TL,Lin CP,Hui CF,Hwang J

更新日期：1999-10-01 00:00:00

abstract：:A novel and efficient screening method for pyrroloquinoline quinone (PQQ) high-yielding methylotrophic strains was developed by using glucose dehydrogenase apoenzyme (GDHA) which depended on PQQ as the cofactor. Using this high-throughput method, PQQ high-yielding strains were rapidly screened out from thousands of me...

journal_title：Applied microbiology and biotechnology

authors： Si Z,Zhu J,Wang W,Huang L,Wei P,Cai J,Xu Z

更新日期：2016-12-01 00:00:00

abstract：:Streptomyces coelicolor A3(2) is a rubber-degrading actinomycete that harbors one gene coding for a latex clearing protein (lcpA3(2)). Within the genome of S. coelicolor A3(2), we identified a gene coding for a novel protein of the TetR family (LcpRBA3(2)) downstream of lcpA3(2) and demonstrated its binding upstream o...

journal_title：Applied microbiology and biotechnology

authors： Coenen A,Oetermann S,Steinbüchel A

更新日期：2019-07-01 00:00:00

abstract：:Escherichia coli normally cannot utilize the β-glucoside sugar cellobiose as a carbon and energy source unless a stringent selection pressure for survival is present. The cellobiose-utilization phenotype can be conferred by mutations in the two cryptic operons, chb and asc. In this study, the cellobiose-utilization ph...

journal_title：Applied microbiology and biotechnology

authors： Vinuselvi P,Lee SK

更新日期：2011-10-01 00:00:00