Isolation and characterization of cold-active family VIII esterases from an arctic soil metagenome.


:Functional screening for lipolytic enzymes at low temperatures resulted in the isolation of the novel cold-active esterases, EstM-N1 and EstM-N2, from a metagenomic DNA library of arctic soil samples. EstM-N1 and EstM-N2 were 395 and 407 amino acids in length, respectively, and showed the highest similarity to class C β-lactamases. However, they shared a relatively low level of sequence similarity (30%) with each other. Phylogenetic analysis of bacterial lipolytic enzymes confirmed that EstM-N1 and EstM-N2 belonged to family VIII of bacterial esterases/lipases. The (His)(6)-tagged esterases were purified to about 99% homogeneity from the soluble fraction of recombinant Escherichia coli cultures. The purified EstM-N1 and EstM-N2 retained more than 50% of maximal activity in the temperature range of 0-35 °C, with optimal temperatures of 20 °C and 30 °C, respectively. Both enzymes preferred the short acyl chains of p-nitrophenyl esters and exhibited very narrow substrate specificity, indicating that they are typical esterases. The β-lactamase activity of EstM-N1 and EstM-N2 was also detected and reached about 31% and 13% of the positive control enzyme, Bacillus cereus β-lactamase, respectively. These first cold-active esterases belonging to family VIII are expected to be useful for potential biotechnological applications as interesting biocatalysts.


Yu EY,Kwon MA,Lee M,Oh JY,Choi JE,Lee JY,Song BK,Hahm DH,Song JK




Has Abstract


2011-04-01 00:00:00












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    authors: Zverlov VV,Velikodvorskaya GA,Schwarz WH,Kellermann J,Staudenbauer WL

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  • L-valine, an antialgal amino acid from Streptomyces jiujiangensis JXJ 0074(T).

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    authors: Zhang BH,Chen W,Li HQ,Yang JY,Zha DM,Duan YQ,N Hozzein W,Xiao M,Gao R,Li WJ

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  • Human acetyl-CoA carboxylase 2 expressed in silkworm Bombyx mori exhibits posttranslational biotinylation and phosphorylation.

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    authors: Hwang IW,Makishima Y,Kato T,Park S,Terzic A,Park EY

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    journal_title:Applied microbiology and biotechnology

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  • Transposon sequencing: methods and expanding applications.

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    journal_title:Applied microbiology and biotechnology

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    authors: Zhang C,Tao T,Ying Q,Zhang D,Lu F,Bie X,Lu Z

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    pub_type: 杂志文章


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    pub_type: 杂志文章


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    pub_type: 杂志文章


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    pub_type: 杂志文章


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    pub_type: 杂志文章,评审


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