Comparative study of the relationship between monomer structure and reactivity for two polyhydroxyalkanoate synthases.

abstract：:Functional screening for lipolytic enzymes at low temperatures resulted in the isolation of the novel cold-active esterases, EstM-N1 and EstM-N2, from a metagenomic DNA library of arctic soil samples. EstM-N1 and EstM-N2 were 395 and 407 amino acids in length, respectively, and showed the highest similarity to class C...

journal_title：Applied microbiology and biotechnology

authors： Yu EY,Kwon MA,Lee M,Oh JY,Choi JE,Lee JY,Song BK,Hahm DH,Song JK

更新日期：2011-04-01 00:00:00

abstract：:To meet the demand for sustainable energy, aquifer thermal energy storage (ATES) is widely used in the subsurface in urban areas. However, contamination of groundwater, especially with chlorinated volatile organic compounds (CVOCs), is often being encountered. This is commonly seen as an impediment to ATES implementat...

journal_title：Applied microbiology and biotechnology

authors： Ni Z,van Gaans P,Smit M,Rijnaarts H,Grotenhuis T

更新日期：2016-04-01 00:00:00

abstract：:Epidermal growth factor (EGF) ameliorates stress and prevents incomplete gastrointestinal development in early-weaned piglets in commercial swine farming. This study aimed to further analyze the biological activities of intracellularly expressed EGF (IE-EGF), extracellularly expressed EGF (EE-EGF), and tagged EGF (T-E...

journal_title：Applied microbiology and biotechnology

authors： Wang S,Guo C,Zhou L,Zhang Z,Huang Y,Yang J,Bai X,Yang K

更新日期：2015-09-01 00:00:00

abstract：:Cellobiose dehydrogenases (CDHs) are extracellular glycosylated haemoflavoenzymes produced by many different wood-degrading and phytopathogenic fungi. Putative cellobiose dehydrogenase genes are recurrently discovered by genome sequencing projects in various phylogenetically distinct fungi. The genomes from the basidi...

journal_title：Applied microbiology and biotechnology

authors： Turbe-Doan A,Arfi Y,Record E,Estrada-Alvarado I,Levasseur A

更新日期：2013-06-01 00:00:00

abstract：:In industry, filamentous fungi have a prominent position as producers of economically relevant primary or secondary metabolites. Particularly, the advent of genetic engineering of filamentous fungi has led to a growing number of molecular tools to adopt filamentous fungi for biotechnical applications. Here, we summari...

journal_title：Applied microbiology and biotechnology

authors： Kluge J,Terfehr D,Kück U

更新日期：2018-08-01 00:00:00

abstract：:Although the niche differentiation of anammox bacteria has been extensively observed in a lab-scale reactor, the inherent mechanism for this ecological phenomenon is still elusive. Here, we combined the long-term reactor operation, genome-centered metagenome, and metatranscriptome analyses to gain insight into the sub...

journal_title：Applied microbiology and biotechnology

authors： Zhao Y,Feng Y,Chen L,Niu Z,Liu S

更新日期：2019-10-01 00:00:00

abstract：:In an effort to produce ricinoleic acid (RA), an important natural raw material with great values as a petrochemical replacement, in Schizosaccharomyces pombe, we introduced Claviceps purpurea oleate Δ12-hydroxylase gene (CpFAH12) to S. pombe, putting it under the control of an inducible nmt1 promoter. However, RA was...

journal_title：Applied microbiology and biotechnology

authors： Yazawa H,Holic R,Kumagai H,Uemura H

更新日期：2013-09-01 00:00:00

abstract：:Relative quantification real-time PCR was used to quantify several bacterial species in ruminal samples from two lactating cows, each sampled 3 h after feeding on two successive days. Abundance of each target taxon was calculated as a fraction of the total 16S rRNA gene copies in the samples, using taxon-specific and ...

journal_title：Applied microbiology and biotechnology

authors： Stevenson DM,Weimer PJ

更新日期：2007-05-01 00:00:00

abstract：:Actinomycetes isolated from Iran soil habitats were tested for the capacity to produce compounds which can protect neurons from cell death generated by oxidative stress in NT2 neurons. Confirmation of our initial hit was accomplished via the determination of amyloid beta level using the enzyme-linked immunosorbent ass...

journal_title：Applied microbiology and biotechnology

authors： Eftekharzadeh B,Hamedi J,Mohammadipanah F,Khodagholi F,Maghsoudi N,Klenk HP

更新日期：2010-05-01 00:00:00

abstract：:During the process of producing cassava starch from Manihot esculenta roots, large amounts of cyanoglycosides were released, which rapidly decayed to CN- following enzymatic hydrolysis. Depending on the varying cyanoglycoside content of the cassava varieties, the cyanide concentration in the wastewater was as high as ...

journal_title：Applied microbiology and biotechnology

authors： Siller H,Winter J

更新日期：1998-02-01 00:00:00

abstract：:The response behavior of three dissimilatory perchlorate-reducing bacteria to different electron acceptors (nitrate, chlorate, and perchlorate) was investigated with two different assays. The observed response was species-specific, dependent on the prior growth conditions, and was inhibited by oxygen. We observed attr...

journal_title：Applied microbiology and biotechnology

authors： Sun Y,Gustavson RL,Ali N,Weber KA,Westphal LL,Coates JD

更新日期：2009-10-01 00:00:00

abstract：:Alcaligenesfaecalis (CCT 7145) was isolated from an Amazonian soil sample after an enrichment process to select for phenol-degrading microorganisms. The isolate was labeled with the green fluorescent protein (gfp) gene. The gfp-transformed cells were easily detected using a hand-held UV transilluminator and their taxo...

journal_title：Applied microbiology and biotechnology

authors： Bastos AE,Cassidy MB,Trevors JT,Lee H,Rossi A

更新日期：2001-07-01 00:00:00

abstract：:Nitrate, injected into oil fields, can oxidize sulfide formed by sulfate-reducing bacteria (SRB) through the action of nitrate-reducing sulfide-oxidizing bacteria (NR-SOB). When reservoir rock contains siderite (FeCO(3)), the sulfide formed is immobilized as iron sulfide minerals, e.g. mackinawite (FeS). The aim of ou...

journal_title：Applied microbiology and biotechnology

authors： Lin S,Krause F,Voordouw G

更新日期：2009-05-01 00:00:00

abstract：:Four potential dehydrogenases identified through literature and bioinformatic searches were tested for L-arabonate production from L-arabinose in the yeast Saccharomyces cerevisiae. The most efficient enzyme, annotated as a D-galactose 1-dehydrogenase from the pea root nodule bacterium Rhizobium leguminosarum bv. trif...

journal_title：Applied microbiology and biotechnology

authors： Aro-Kärkkäinen N,Toivari M,Maaheimo H,Ylilauri M,Pentikäinen OT,Andberg M,Oja M,Penttilä M,Wiebe MG,Ruohonen L,Koivula A

更新日期：2014-12-01 00:00:00

abstract：:The biomass carbon ratios of biochemicals related to biomass have been reviewed. Commercial products from biomass were explained. The biomass carbon ratios of biochemical compounds were measured by accelerator mass spectrometry (AMS) based on the (14)C concentration of carbons in the compounds. This measuring method u...

journal_title：Applied microbiology and biotechnology

authors： Kunioka M

更新日期：2010-06-01 00:00:00

abstract：:In Catharanthus roseus cell cultures the time courses of four enzyme activities, tryptophan decarboxylase (TDC), strictosidine synthase (SSS), geraniol-10-hydroxylase (G10H) and anthranilate synthase (AS), and alkaloid accumulation were compared under two different culture conditions (low-inoculum density and high-ino...

journal_title：Applied microbiology and biotechnology

authors： Moreno PR,Schlatmann JE,van der Heijden R,van Gulik WM,ten Hoopen HJ,Verpoorte R,Heijnen JJ

更新日期：1993-04-01 00:00:00

abstract：:Fungal interspecific interactions enhance biosynthesis of phenylpropanoid metabolites (PM), and production of nitric oxide (NO) is known to be involved in this process. However, it remains unknown which signaling pathway(s) or regulator(s) mediate fungal PM biosynthesis. In this study, we cocultured two white-rot fung...

journal_title：Applied microbiology and biotechnology

authors： Zhao Y,Xi Q,Xu Q,He M,Ding J,Dai Y,Keller NP,Zheng W

更新日期：2015-05-01 00:00:00

abstract：:To clarify the effect of type of foods on the intestinal environment, Far East Asian- (FEA; rich in rice starch, soy protein and soy oil) and Far East Asian marine- (FEAM; rich in rice starch, fish meal, fish oil and brown alga) modelled diets and sucrose, casein and beef tallow-rich (SCB) diet were prepared. After th...

journal_title：Applied microbiology and biotechnology

authors： An C,Kuda T,Yazaki T,Takahashi H,Kimura B

更新日期：2014-05-01 00:00:00

abstract：:Wood contains primary extractives, which are present in all woods, and secondary extractives, which are confined in certain wood species. Extractives in wood play a major role in wood-bonding processes, as they can contribute to or determine the bonding relevant properties of wood such as acidity and wettability. Ther...

journal_title：Applied microbiology and biotechnology

authors： Roffael E

更新日期：2016-02-01 00:00:00

abstract：:Recently, it was shown that electrical DNA-chips in connection with a magnetic bead-based sandwich-hybridization assay can be a suitable alternative for the analysis of gene expression by monitoring the respective mRNA levels. In this study, we established an improved assay which allowed for a significantly shortened ...

journal_title：Applied microbiology and biotechnology

authors： Pioch D,Jürgen B,Evers S,Maurer KH,Hecker M,Schweder T

更新日期：2008-03-01 00:00:00

abstract：:The 454 pyrosequencing technique was applied to evaluate microbial community composition in sediment and water samples collected from the river receiving effluents from a swine farm and a farmhouse restaurant, respectively. For each sample, 4,600 effective sequences were selected and used to do the bacterial diversity...

journal_title：Applied microbiology and biotechnology

authors： Lu XM,Lu PZ

更新日期：2014-09-01 00:00:00

abstract：:Dengue virus (DENV) and Japanese encephalitis virus (JEV) are closely related mosquito-borne flaviviruses. Together, they caused most arthropod-borne diseases in the world. Previously, we had demonstrated that both live attenuated and inactivated JE vaccines elicited cross-protection against DENV infection, and a DNA ...

journal_title：Applied microbiology and biotechnology

authors： Gao N,Li J,Sheng Z,Chen H,Fan D,Wang P,An J

更新日期：2019-06-01 00:00:00

abstract：:The microbial production of biofuels is a promising avenue for the development of viable processes for the generation of fuels from sustainable resources. In order to become cost and energy effective, these processes must utilize organisms that can be optimized to efficiently produce candidate fuels from a variety of ...

journal_title：Applied microbiology and biotechnology

authors： Clomburg JM,Gonzalez R

更新日期：2010-03-01 00:00:00

abstract：:Cytidine diphosphate choline (CDP-choline) has been applied for treating acute craniocerebral injury and allowing recovery of consciousness after brain surgery. In this study, an acetate kinase (ACK)/acetyl phosphate system was used to supply ATP and combined with Escherichia coli-overexpressed CMP kinase (CMK), NDP k...

journal_title：Applied microbiology and biotechnology

authors： Liu Y,Wang J,Xu C,Chen Y,Yang J,Liu D,Niu H,Jiang Y,Yang S,Ying H

更新日期：2017-02-01 00:00:00

abstract：:The use of crude aqueous extracts of Cynara cardunculus flowers as coagulants in the production of high-quality sheep and goat cheeses-as are the cases of several Portuguese and Spanish cheese varieties with Protected Designation of Origin status-has been maintained since ancient times. The unique rheological attribut...

journal_title：Applied microbiology and biotechnology

authors： Almeida CM,Simões I

更新日期：2018-06-01 00:00:00

abstract：:Cyanobacterial blooms have become a serious problem in Lake Taihu during the last 20 years, and Microcystis aeruginosa and Synechococcus sp. are the two dominant species in cyanobacterial blooms of Lake Taihu. A freshwater bacterial strain, Shewanella sp. Lzh-2, with strong algicidal properties against harmful cyanoba...

journal_title：Applied microbiology and biotechnology

authors： Li Z,Lin S,Liu X,Tan J,Pan J,Yang H

更新日期：2014-05-01 00:00:00

abstract：:The morbidity and mortality associated with bacterial infections have remained significant despite chemotherapeutic advances. With the emergence of drug-resistant bacterial strains, the situation has become a serious threat to the public health. Thus, there is an urgent need to identify novel antibacterials. The major...

journal_title：Applied microbiology and biotechnology

authors： Akbar N,Siddiqui R,Sagathevan KA,Khan NA

更新日期：2019-05-01 00:00:00

abstract：:Since 2015, outbreaks of hepatitis-hydropericardium syndrome (HPS) caused by a novel genotype of fowl adenovirus 4 (FAdV-4) infection have created serious economic losses in China. Given that other serotypes of hypervirulent FAdVs have also been reported in poultry around the world, a common ELISA for all serotypes wi...

journal_title：Applied microbiology and biotechnology

authors： Pan Q,Wang J,Gao Y,Cui H,Liu C,Qi X,Zhang Y,Wang Y,Li K,Gao L,Wang X

更新日期：2020-01-01 00:00:00

abstract：:Biosynthesis of Ds(+)-threo-isocitric acid from ethanol in the Yarrowia lipolytica batch and repeated-batch cultures was studied. Repeated-batch cultivation was found to provide for a good biosynthetic efficiency of the producer for as long as 748 h, probably due to maintenance of high activities of enzymes involved i...

journal_title：Applied microbiology and biotechnology

authors： Morgunov IG,Kamzolova SV,Karpukhina OV,Bokieva SB,Inozemtsev AN

更新日期：2019-04-01 00:00:00

abstract：:A flocculent Saccharomyces cerevisiae strain with the ability to express both the LAC4 (coding for beta-galactosidase) and LAC12 (coding for lactose permease) genes of Kluyveromyces marxianus was constructed. This recombinant strain is not only able to grow on lactose, but it can also ferment this substrate. To our kn...

journal_title：Applied microbiology and biotechnology

authors： Domingues L,Teixeira JA,Lima N

更新日期：1999-05-01 00:00:00