Abstract：
:A flocculent Saccharomyces cerevisiae strain with the ability to express both the LAC4 (coding for beta-galactosidase) and LAC12 (coding for lactose permease) genes of Kluyveromyces marxianus was constructed. This recombinant strain is not only able to grow on lactose, but it can also ferment this substrate. To our knowledge this is the first time that a recombinant S. cervisiae has been found to ferment lactose in a way comparable to that of the existing lactose-fermenting yeast strains. Moreover, the flocculating capacity of the strain used in this work gives the process several advantages. On the one hand, it allows for operation in a continuous mode at high cell concentration, thus increasing the system's overall productivity; on the other hand, the biomass concentration in the effluent is reduced, thus decreasing product separation/purification costs.
journal_name
Appl Microbiol Biotechnoljournal_title
Applied microbiology and biotechnologyauthors
Domingues L,Teixeira JA,Lima Ndoi
10.1007/s002530051441subject
Has Abstractpub_date
1999-05-01 00:00:00pages
621-6issue
5eissn
0175-7598issn
1432-0614journal_volume
51pub_type
杂志文章abstract：:In this study, we used the non-carotenogenic yeast Pichia pastoris X33 as a receptor for β-carotene-encoding genes, in order to obtain new recombinant strains capable of producing different carotenoidic compounds. We designed and constructed two plasmids, pGAPZA-EBI* and pGAPZA-EBI*L*, containing the genes encoding ly...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-011-3764-7
更新日期：2012-03-01 00:00:00
abstract：:Superoxide dismutase (SOD) activity is one major defense line against oxidative stress for all of the aerobic organisms, and industrial production of this enzyme is highly demanded. The Cu/Zn superoxide dismutase gene (KmSOD1) of Kluyveromyces marxianus L3 was cloned and characterized. The deduced KmSod1p protein shar...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-007-1270-8
更新日期：2008-01-01 00:00:00
abstract：:Significant decreases in the apparent viscosity of a bacterial biofilm suspension were measured following addition of sodium, potassium, magnesium, or calcium salts, whereas iron salts increased the viscosity. Electrostatic interactions contribute to biofilm cohesion and iron cations are potent crosslinkers of the bio...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-002-1044-2
更新日期：2002-09-01 00:00:00
abstract：:Programmable transcriptional regulation is a powerful tool to study gene functions. Current methods to selectively regulate target genes are mainly based on promoter exchange or on overexpressing transcriptional activators. To expand the discovery toolbox, we designed a dCas9-based RNA-guided synthetic transcription a...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-020-10900-9
更新日期：2020-11-01 00:00:00
abstract：:The essential general features required for lipid membranes of extremophilic archaea to fulfill biological functions are that they are in the liquid crystalline phase and have extremely low permeability of solutes that is much less temperature sensitive due to a lack of lipid-phase transition and highly branched isopr...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s00253-009-2102-9
更新日期：2009-08-01 00:00:00
abstract：:Ethidium monoazide bromide (EMA) treatment of pure culture and environmental waters at low concentrations (1.0-7.5 microg/ml) indicated effective enumeration of viable and viable but nonculturable Escherichia coli in pure cultures, creek waters, and secondary activated sludge effluent samples by quantitative polymeras...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-008-1846-y
更新日期：2009-03-01 00:00:00
abstract：:The Vitreoscilla hemoglobin (VHb) gene (vgb) was integrated into the chromosome of Bacillus thuringiensis BMB171 using integrative vector pEG491. The production of VHb was confirmed by CO-difference spectra analysis. Fermentation experiments results showed that with the production of VHb, the critical oxygen concentra...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-006-0676-z
更新日期：2007-02-01 00:00:00
abstract：:Sialylation affects circulating half-life, charge distribution, and other biochemical properties of therapeutic glycoproteins. Loss of protein sialylation during glycoprotein-producing bioprocesses could lead to a low final protein sialylation level and bring negative effects on subsequent clinical efficacy. In this w...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-019-09850-8
更新日期：2019-06-01 00:00:00
abstract：:The main objective of this work was the optimization of the production of the beta-ketolase, acetopyruvate hydrolase, from Pseudomonas putida O1. Orcinol was used as an inducer for enzyme production. The growth medium was optimized in two steps. In the first step, screening for optimal glucose concentration was perfor...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-002-1133-2
更新日期：2002-11-01 00:00:00
abstract：:Due to efficient photosynthetic capability, robust growth, and clear genetic background, cyanobacteria are recently used for production of different biofuel and biochemical molecules by genetic engineering and showed great potentials as the next-generation microbial cell factory. For improving the production of bio-pr...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-013-4837-6
更新日期：2013-07-01 00:00:00
abstract：:The inhibitory effects of aqueous, ethanolic and dichloromethane fractions from 16 marine algae from the Atlantic shores of North-East Brittany, France, have been investigated against microorganisms frequently associated with immersed surfaces. The extracts were tested in vitro against isolates of marine fungi, bacter...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s002530000413
更新日期：2000-10-01 00:00:00
abstract：:Simultaneous isomerisation and fermentation (SIF) of xylose and simultaneous isomerisation and cofermentation (SICF) of a glucose/xylose mixture was carried out by Saccharomyces cerevisiae in the presence of xylose isomerase. The SIF of 50 g I(-1) xylose gave an ethanol concentration and metabolic yield of 7.5 g l(-1)...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s002530050025
更新日期：2000-03-01 00:00:00
abstract：:We report the study of the dynamics of substrate utilization by the genetic modified strain Yarrowia lipolytica H222-S4(p67ICL1) T5. In contrast to its wild-type equivalent, this recombinant strain is able to excrete the sucrose cleaving enzyme invertase. Both the sucrose degradation rate and the glucose and fructose ...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-011-3681-9
更新日期：2012-02-01 00:00:00
abstract：:The gene celB encoding an endoglucanase from Paenibacillus sp. BP-23 was cloned and expressed in Escherichia coli. The nucleotide sequence of a 4161 bp DNA fragment containing the celB gene was determined, revealing an open reading frame of 2991 nucleotides that encodes a protein of 106,927 Da. Comparison of the deduc...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s002530000470
更新日期：2001-01-01 00:00:00
abstract：:The transport of glucose by the solventogenic anaerobe Clostridium acetobutylicum was investigated. Glucose phosphoenolpyruvate (PEP)-dependent phosphotransferase system (PTS) activity was detected in extracts prepared from cultures grown on glucose and extract fractionation revealed that both soluble and membrane com...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-006-0679-9
更新日期：2007-02-01 00:00:00
abstract：:Thiabendazole (TBZ) is a fungicide used in fruit-packaging plants. Its application leads to the production of wastewaters requiring detoxification. In the absence of efficient treatment methods, biological depuration of these effluents could be a viable alternative. However, nothing is known regarding the microbial de...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-017-8128-5
更新日期：2017-05-01 00:00:00
abstract：:Starting from mature vegetable compost, four bacterial strains were selected using a lignin-rich medium. 16S ribosomal RNA identification of the isolates showed high score similarity with Pseudomonas spp. for three out of four isolates. Further characterization of growth on mixtures of six selected lignin model compou...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-017-8211-y
更新日期：2017-06-01 00:00:00
abstract：:Yeast are important production platforms for the generation of recombinant proteins. Nonetheless, their use has been restricted in the production of therapeutic proteins due to differences in their glycosylation profile with that of higher eukaryotes. The yeast strain Pichia pastoris is an industrially important organ...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-013-5468-7
更新日期：2014-03-01 00:00:00
abstract：:Active penicillin acylase from Kluyvera citrophila strain ATCC 21,285 consists of two different alpha- and beta-subunits derived from a single precursor by post-translational processing. Using the chemical mutagen hydroxylamine we have treated plasmid pYKD59 containing the active penicillin acylase gene (pga) from K. ...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/BF00183245
更新日期：1992-02-01 00:00:00
abstract：:A general procedure for the high yield immobilization of enzymes with the help of specific anti-enzyme antibodies is described. Polyclonal antibodies were raised against Aspergillus niger glucose oxidase and horseradish peroxidase in rabbits and the gamma globulin (IgG) fraction from the immune sera isolated by ammoni...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s002530051534
更新日期：1999-09-01 00:00:00
abstract：:Nitric oxide (NO) is known to be involved in modulating production of styrylpyrone polyphenols in the basidiomycete Inonotus obliquus. However, it remains unknown how NO orchestrates fungal styrylpyrone biosynthesis. Here, we show that a transient NO burst correlated with an enhanced expression of phenylalanine ammoni...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-016-7442-7
更新日期：2016-05-01 00:00:00
abstract：:A cDNA fragment encoding the A catalytic domain of the Neocallimastix frontalis endoxylanase XYN3 was amplified and cloned by the polymerase chain reaction technique. The xyn3A DNA fragment was inserted between the Saccharomyces cerevisiae phosphoglycerate kinase gene promoter and terminator sequences on a multicopy e...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s002530051510
更新日期：1999-08-01 00:00:00
abstract：:Autohydrogenotrophic batch growth of Ralstonia eutropha H16 was studied in a stirred-tank reactor with nitrate and nitrite as terminal electron acceptors and the sole limiting substrates. Assuming product inhibition by nitrite, saturation kinetics with the two limiting substrates and a simple switching function, which...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-007-0983-z
更新日期：2007-08-01 00:00:00
abstract：:A gene for a synthetic protein-based polymer, G-(VPGVG)119-VPGV, coding for the EG-120mer (elastomer), was cloned into a fungal expression vector to allow constitutive expression of the polymer controlled by the gpdA (glyceraldehyde-3-phosphate dehydrogenase) promoter sequence of Aspergillus nidulans. Stable transform...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s002530050942
更新日期：1997-04-01 00:00:00
abstract：:Nervonic acid (NA) is a major very long-chain monounsaturated fatty acid found in the white matter of mammalian brains, which plays a critical role in the treatment of psychotic disorders and neurological development. In the nature, NA has been synthesized by a handful plants, fungi, and microalgae. Although the metab...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s00253-018-8859-y
更新日期：2018-04-01 00:00:00
abstract：:In this study, the kinetic behaviors between acetone and composite bead were investigated. The microbial growth rate decreased with increasing average inlet concentration and increased with increasing operation temperature at average inlet concentration ranging from 50 to 300 ppm and operation temperature ranging from...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-005-0236-y
更新日期：2006-08-01 00:00:00
abstract：:Pseudomonas sp. D7-4 and Pseudomonas sp. B13 FR1(pFRC20P) degraded mixtures of chloro- and methyl-substituted benzoates exclusively via an extended ortho pathway, whereas in Pseudomonas putida WR 201 both ortho and meta fission were induced by mixtures of 3-chloro- and 3-methylbenzoate or even by 3-chloro-benzoate alo...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s002530051282
更新日期：1998-08-01 00:00:00
abstract：:Human epidermal growth factor receptors (EGFR) are an important target in drug discovery in terms of both protein-small-molecule interactions and protein-protein interactions. In this work, the isolation of a stable soluble protein of the tyrosine kinase domain of EGFR in Escherichia coli expression has been accomplis...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-019-10116-6
更新日期：2019-10-01 00:00:00
abstract：:The most notable microbial survival models of disinfection kinetics are the original and modified versions of the static Chick-Watson-Hom's (CWH) initially developed for water chlorination. They can all be viewed as special cases of the Weibull survival model, where the observed static curve is the cumulative form (CD...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s00253-020-11042-8
更新日期：2021-01-01 00:00:00
abstract：:Developing the production of ectomycorrhizal (ECM) mushrooms in forest has become a challenge. Only a few ECM species are currently cultivable. Controlled mycorrhization practices offer promising advance to produce currently uncultivable ECM mushrooms. The persistence of the production of edible species, either cultiv...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s00253-010-3022-4
更新日期：2011-02-01 00:00:00
