Abstract:
:A gene for a synthetic protein-based polymer, G-(VPGVG)119-VPGV, coding for the EG-120mer (elastomer), was cloned into a fungal expression vector to allow constitutive expression of the polymer controlled by the gpdA (glyceraldehyde-3-phosphate dehydrogenase) promoter sequence of Aspergillus nidulans. Stable transformants of A. nidulans showed plasmid integration with varying copy number when analyzed by Southern-blot hybridization. Expression of the synthetic gene was demonstrated by Northern-blot hybridization. However, the translational efficiency for production of the polymer polypeptide was low, presumably because of certain codons in the polymer gene (CCG and GUA) that are rarely used by A. nidulans. Partial purification by reversible phase transition followed by sodium dodecyl sulfate/polyacrylamide gel electrophoresis revealed the presence of polymer protein in a transformant that contained multiple copies of the polymer gene. This study represents the first attempt to express a synthetic gene (with no natural analog) in a fungus.
journal_name
Appl Microbiol Biotechnoljournal_title
Applied microbiology and biotechnologyauthors
Herzog RW,Singh NK,Urry DW,Daniell Hdoi
10.1007/s002530050942subject
Has Abstractpub_date
1997-04-01 00:00:00pages
368-72issue
4eissn
0175-7598issn
1432-0614journal_volume
47pub_type
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更新日期:1999-03-01 00:00:00
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journal_title:Applied microbiology and biotechnology
pub_type: 杂志文章,评审
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journal_title:Applied microbiology and biotechnology
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更新日期:2017-05-01 00:00:00
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更新日期:2013-03-01 00:00:00