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Simultaneous bioconversion of glucose and xylose to ethanol by Saccharomyces cerevisiae in the presence of xylose isomerase.

Abstract：

:Simultaneous isomerisation and fermentation (SIF) of xylose and simultaneous isomerisation and cofermentation (SICF) of a glucose/xylose mixture was carried out by Saccharomyces cerevisiae in the presence of xylose isomerase. The SIF of 50 g I(-1) xylose gave an ethanol concentration and metabolic yield of 7.5 g l(-1) and 0.36 g (g xylose consumed)(-1). These parameters improved to 13.4 g l(-1) and 0.40 respectively, when borate was added to the medium. The SICF of a mixture of 50 g l(-1) glucose and 50 g l(-1) xylose gave an ethanol concentration and metabolic yield of 29.8 g l(-1) and 0.42 respectively, in the presence of borate. Temperature modulation from 30 degrees C to 35 degrees C during fermentation further enhanced the above parameters to 39 g l(-1) and 0.45 respectively. The approach was extended to the bioconversion of sugars present in a real lignocellulose hydrolysate (peanut-shell hydrolysate) to ethanol, with a fairly good yield.

journal_name

Appl Microbiol Biotechnol

journal_title

Applied microbiology and biotechnology

authors

Chandrakant P,Bisaria VS

doi

10.1007/s002530050025

subject

Has Abstract

pub_date

2000-03-01 00:00:00

pages

301-9

issue

3

eissn

0175-7598

issn

1432-0614

journal_volume

53

pub_type

杂志文章

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