Molecular cloning and characterization of a multidomain endoglucanase from Paenibacillus sp BP-23: evaluation of its performance in pulp refining.

abstract：:Carotenoids such as beta-carotene, lycopene, and antheraxanthin have plenty of scientific and commercial value. The comprehensive investigation of carotenoids drives people to improve and develop all kinds of analytical techniques to approach or even achieve "versatile" analysis. The metabolic engineering efforts in p...

journal_title：Applied microbiology and biotechnology

authors： Liu GN,Zhu YH,Jiang JG

更新日期：2009-07-01 00:00:00

abstract：:Seed-based expression system is an attractive platform for the production of recombinant proteins in molecular farming. Despite the many advantages of molecular farming, little is known about the effect of the different subcellular accumulation of recombinant proteins on the endoplasmic reticulum (ER) quality control ...

journal_title：Applied microbiology and biotechnology

authors： Wang D,Ma J,Sun D,Li H,Jiang C,Li X

更新日期：2015-08-01 00:00:00

abstract：:The ability of acetic acid bacteria (AAB) to produce cellulose has gained much industrial interest due to the physical and chemical characteristics of bacterial cellulose. The production of cellulose occurs in the presence of oxygen and in a glucose-containing medium, but it can also occur during vinegar elaboration b...

journal_title：Applied microbiology and biotechnology

authors： Valera MJ,Torija MJ,Mas A,Mateo E

更新日期：2015-02-01 00:00:00

abstract：:The synergistic effect between polyoxometalates (POMs), namely K(5)[SiW(11)V(V)O(40)]·11H(2)O and H(5)[PMo(10)V(V) (2)O(40)]·13H(2)O and laccase from ascomycete Myceliophthora thermophila has been employed for the first time in oxidative polymerization of catechol. Such a laccase-mediator system allowed the formation ...

journal_title：Applied microbiology and biotechnology

authors： Kim S,Silva C,Evtuguin DV,Gamelas JA,Cavaco-Paulo A

更新日期：2011-02-01 00:00:00

abstract：:Cultures of Umbelopsis ramanniana (=Mucor ramannianus) were grown in fluid Sabouraud medium for 3 days, dosed with 0.23 mM benzo[f]quinoline, benzo[h]quinoline, or phenanthridine (benzo[c]quinoline), and incubated for another 18 days. Cultures were extracted and metabolites (66-75% of the UV absorbance) were separated...

journal_title：Applied microbiology and biotechnology

authors： Sutherland JB,Cross EL,Heinze TM,Freeman JP,Moody JD

更新日期：2005-05-01 00:00:00

abstract：:In microorganisms, the enzyme acetate kinase (AK) catalyses the formation of ATP from ADP by de-phosphorylation of acetyl phosphate into acetic acid. A mutant strain of Clostridium acetobutylicum lacking acetate kinase activity is expected to have reduced acetate and acetone production compared to the wild type. In th...

journal_title：Applied microbiology and biotechnology

authors： Kuit W,Minton NP,López-Contreras AM,Eggink G

更新日期：2012-05-01 00:00:00

abstract：:D-Danshensu (D-DSS), a traditional Chinese medicine, is used to treat cardiovascular and cerebrovascular diseases. However, current isolation protocols for D-DSS both natural and synthetic are not ideal; therefore, in this study, we have developed a whole-cell biotransformation method to produce D-DSS from L-DOPA. Thi...

journal_title：Applied microbiology and biotechnology

authors： Xiong T,Jiang J,Bai Y,Fan TP,Zhao Y,Zheng X,Cai Y

更新日期：2019-08-01 00:00:00

abstract：:Residual biomass, produced by the thermophilic fungus, Talaromyces emersonii CBS 814.70, following growth on glucose-containing media, was examined for its ability to take up uranium from aqueous solution. It was found that the biomass had a relatively high observed biosorption capacity for the uranium (280 mg/g dry w...

journal_title：Applied microbiology and biotechnology

authors： Bengtsson L,Johansson B,Hackett TJ,McHale L,McHale AP

更新日期：1995-01-01 00:00:00

abstract：:The exclusive use of isopropyl beta-D-thiogalactopyranoside to activate the T7 promoter for protein production has limited the general use of the expression system. We have sought an alternative by constructing a recombinant Escherichia coli strain, BL21 (G2), to carry a chromosomal copy of T7 gene 1 fused to the lamb...

journal_title：Applied microbiology and biotechnology

authors： Chao YP,Law W,Chen PT,Hung WB

更新日期：2002-03-01 00:00:00

abstract：:Bacteria from the genus Geobacillus are generally obligately thermophilic, with a unique bioenergy production capacity and unique enzymes. Geobacillus species were isolated primarily from hot springs, oilfields, and associated soils. They often exhibit unique survival patterns in these extreme oligotrophic environment...

journal_title：Applied microbiology and biotechnology

authors： Lin JH,Zhang KC,Tao WY,Wang D,Li S

更新日期：2019-10-01 00:00:00

abstract：:In this study, we aimed at determining the impact of naphthalene and different oxygen levels on a biofilm bacterial community originated from a petroleum hydrocarbon-contaminated groundwater. By using cultivation-dependent and cultivation-independent approaches, the enrichment, identification, and isolation of aerobic...

journal_title：Applied microbiology and biotechnology

authors： Benedek T,Szentgyörgyi F,Szabó I,Farkas M,Duran R,Kriszt B,Táncsics A

更新日期：2020-07-01 00:00:00

abstract：:Two compost piles were prepared, using two ventilation systems: forced ventilation and ventilation through mechanical turning. The material to compost was a mixture of orange waste, olive pomace, and grass clippings (2:1:1 v/v). During the composting period (375 days), samples were periodically taken from both piles, ...

journal_title：Applied microbiology and biotechnology

authors： Coelho L,Reis M,Dionísio L

更新日期：2013-05-01 00:00:00

abstract：:Baicalin (baicalein 7-O-β-D-glucuronide) is one of the major flavonoid glucuronides found in traditional herbal medicines. Because its aglycone, baicalein, is absorbed more quickly and shows more effective properties than baicalin, the conversion of baicalin into baicalein by β-glucuronidase (GUS) has drawn the attent...

journal_title：Applied microbiology and biotechnology

authors： Sakurama H,Kishino S,Uchibori Y,Yonejima Y,Ashida H,Kita K,Takahashi S,Ogawa J

更新日期：2014-05-01 00:00:00

abstract：:Xanthomonas campestris pv. campestris, the causal agent of black-rot disease of cruciferous plants, and an important industrial microbe, was able to express the Escherichia coli beta-glucuronidase reporter gene (uidA) when fused to the E. coli lactose operon promoter on a wide-host-range plasmid vector. The gene fusio...

journal_title：Applied microbiology and biotechnology

authors： Soby SD,Daniels MJ

更新日期：1996-12-01 00:00:00

abstract：:Secretion of fusion proteins composed of cytoplasmic protein dihydrofolate reductase (DHFR) and the Escherichia coli alpha-haemolysin (HlyA) C-terminal sequence was examined through the haemolysin secretion machinery of E. coli. DHFR of various lengths was combined with the HlyA C-terminal region, and both secretion a...

journal_title：Applied microbiology and biotechnology

authors： Nakano H,Kawakami Y,Nishimura H

更新日期：1992-09-01 00:00:00

abstract：:Human neutrophil peptide 1 (HNP1) is a small (3.44 kDa) cationic peptide that is a distinct member of the defensin family. HNP1 plays a crucial role in controlling bacterial infections, particularly by antibiotic-resistant bacteria, through membrane perforation patterns. The structural characteristics of HNP1's three ...

journal_title：Applied microbiology and biotechnology

authors： Zhang X,Jiang A,Qi B,Yu H,Xiong Y,Zhou G,Qin M,Dou J,Wang J

更新日期：2018-06-01 00:00:00

abstract：:A cytoplasmic NADH oxidase (NOX) was purified from a soil bacteria, Brevibacterium sp. KU1309, which is able to grow in the medium containing 2-phenylethanol as the sole source of carbon under an aerobic condition. The enzyme catalyzed the oxidation of NADH to NAD+ involving two-electron reduction of O2 to H2O2. The m...

journal_title：Applied microbiology and biotechnology

authors： Hirano J,Miyamoto K,Ohta H

更新日期：2008-08-01 00:00:00

abstract：:This paper reports a novel integrated metabolic pathway of amino acid production through the utilization of phenol in Corynebacterium glutamicum. In the presence of 8.5 mM phenol, the level of glutamate and proline production increased up to 1.2- and 14.7-fold, respectively, compared to the control condition. In addit...

journal_title：Applied microbiology and biotechnology

authors： Lee SY,Kim YH,Min J

更新日期：2010-01-01 00:00:00

abstract：:With advances in the development of DNA vaccines and gene therapy, there is a growing need for plasmid DNA with high quality for fundamental research and clinical trials. In this report, a scalable automated process for large-scale preparation of plasmid is described. This process is based on alkaline lysis and can be...

journal_title：Applied microbiology and biotechnology

authors： Li X,Jin H,Wu Z,Rayner S,Yin J,Yu Y,Zhang W,He Z,Wang C,Wang B

更新日期：2007-07-01 00:00:00

abstract：:Sulfide detoxification can be catalyzed by ancient membrane-bound flavoproteins, sulfide:quinone oxidoreductases (Sqr), which have important roles in sulfide homeostasis and sulfide-dependent energy conservation processes by transferring electrons from sulfide to respiratory or photosynthetic membrane electron flow. S...

journal_title：Applied microbiology and biotechnology

authors： Duzs Á,Tóth A,Németh B,Balogh T,Kós PB,Rákhely G

更新日期：2018-06-01 00:00:00

abstract：:Hydroxy fatty acids (HFA) have gained importance because of their special properties such as higher viscosity and reactivity compared with other non-hydroxy fatty acids. The bacterial isolate Pseudomonas aeruginosa (PR3) was reported to produce mono-, di-, and trihydroxy fatty acids from different unsaturated fatty ac...

journal_title：Applied microbiology and biotechnology

authors： Chang IA,Kim IH,Kang SC,Hou CT,Kim HR

更新日期：2007-02-01 00:00:00

abstract：:The production of mannitol by Lactobacillus intermedius NNRL B-3693 using molasses as an inexpensive carbon source was evaluated. The bacterium produced mannitol (104 g/l) from molasses and fructose syrups (1:1; total sugars, 150 g/l; fructose:glucose 4:1) in 16 h. Several kinds of inexpensive organic and inorganic ni...

journal_title：Applied microbiology and biotechnology

authors： Saha BC

更新日期：2006-10-01 00:00:00

abstract：:The catalytic domain encoded by an adenine-thymine (AT)-rich xylanase gene (xynA) of the anaerobic fungus Orpinomyces was expressed in Hypocrea jecorina under the control of the cel7A promoter and terminator. No XynA protein was detected in H. jecorina culture supernatants when the original sequence was fused to the H...

journal_title：Applied microbiology and biotechnology

authors： Li XL,Skory CD,Ximenes EA,Jordan DB,Dien BS,Hughes SR,Cotta MA

更新日期：2007-04-01 00:00:00

abstract：:Phosphate is one of the major constituents in growth media. It closely regulates central carbon and energy metabolism. Biochemical reactions in central carbon metabolism are known to be regulated by phosphorylation and dephosphorylation of enzymes. Phosphate scarcity can limit microbial productivity. However, microorg...

journal_title：Applied microbiology and biotechnology

authors： Pandi K,Chauhan AS,Khan WH,Rathore AS

更新日期：2020-11-01 00:00:00

abstract：:A Lactobacillus brevis strain with the ability to synthesize butanol from glucose was constructed by metabolic engineering. The genes crt, bcd, etfB, etfA, and hbd, composing the bcs-operon, and the thl gene encode the enzymes of the lower part of the clostridial butanol pathway (crotonase, butyryl-CoA-dehydrogenase, ...

journal_title：Applied microbiology and biotechnology

authors： Berezina OV,Zakharova NV,Brandt A,Yarotsky SV,Schwarz WH,Zverlov VV

更新日期：2010-06-01 00:00:00

abstract：:Escherichia coli 044 K74 grown under anaerobic conditions in the presence of L: (-)-carnitine is able to convert D: (+)-carnitine into the L: (-)-enantiomer. This activity is repressed by electron acceptors such as oxygen and nitrate as well as by glucose. D: (+)-Carnitine shows no effect on the induction or repressio...

journal_title：Applied microbiology and biotechnology

authors： Jung H,Kleber HP

更新日期：1991-06-01 00:00:00

abstract：:This study evaluated the effect of inorganic mercury (Hg) on bacterial community and diversity in different soils. Three soils-neutral, alkaline and acidic-were spiked with six different concentrations of Hg ranging from 0 to 200 mg kg-1 and aged for 90 days. At the end of the ageing period, 18 samples from three diff...

journal_title：Applied microbiology and biotechnology

authors： Mahbub KR,Subashchandrabose SR,Krishnan K,Naidu R,Megharaj M

更新日期：2017-03-01 00:00:00

abstract：:An engineered yeast with emission of fluorescence from the cell surface was constructed. Cell surface engineering was applied to display a visible reporter molecule, green fluorescent protein (GFP). A glucose-inducible promoter GAPDH as a model promoter was selected to control the expression of the reporter gene in re...

journal_title：Applied microbiology and biotechnology

authors： Ye K,Shibasaki S,Ueda M,Murai T,Kamasawa N,Osumi M,Shimizu K,Tanaka A

更新日期：2000-07-01 00:00:00

abstract：:Physiological heterogeneity constitutes a critical parameter in biotechnological systems since both metabolite yield and productivity are often hampered by the presence of undesired physiological cell subpopulations. In the present study, the physiological status and functionality of Pseudomonas taetrolens cells were ...

journal_title：Applied microbiology and biotechnology

authors： Alonso S,Rendueles M,Díaz M

更新日期：2012-12-01 00:00:00

abstract：:Microbial transglutaminase (MTG) from Streptomyces mobaraensis has been widely used for crosslinking proteins in order to acquire products with improved properties. To improve the yield and enable a facile and efficient purification process, recombinant vectors, harboring various heterologous signal peptide-encoding f...

journal_title：Applied microbiology and biotechnology

authors： Mu D,Lu J,Qiao M,Kuipers OP,Zhu J,Li X,Yang P,Zhao Y,Luo S,Wu X,Jiang S,Zheng Z

更新日期：2018-07-01 00:00:00