Polyoxometalate/laccase-mediated oxidative polymerization of catechol for textile dyeing.

abstract：:Respiration measurement in shake flasks is introduced as a new method to characterize the metabolic activity of microorganisms during and after stress exposure. The major advantage of the new method is the possibility to determine the metabolic activity independent of manual sampling without the necessity to change th...

journal_title：Applied microbiology and biotechnology

authors： Seletzky JM,Noack U,Fricke J,Hahn S,Büchs J

更新日期：2006-10-01 00:00:00

abstract：:We have characterized a mutant of Streptomyces clavuligerus NRRL 3585 which is almost completely blocked in cephalosporin biosynthesis and exhibits depressed activities of both the delta(L-alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV) synthetase and cyclase enzymes of the cephalosporin pathway. A wild-type DNA region ...

journal_title：Applied microbiology and biotechnology

authors： Piret J,Resendiz B,Mahro B,Zhang JY,Serpe E,Romero J,Connors N,Demain AL

更新日期：1990-02-01 00:00:00

abstract：:Escherichia coli cells expressing mink (Mustela vison) growth hormone were grown in a batch fermentation process. The expression level was estimated to be 27% of the total cellular protein after 3 h of induction with 1 mM isopropyl beta-D-thiogalactoside (IPTG). If the expression of mink growth hormone (mGH) was induc...

journal_title：Applied microbiology and biotechnology

authors： Sereikaite J,Statkute A,Morkunas M,Radzevicius K,Borromeo V,Secchi C,Bumelis VA

更新日期：2007-02-01 00:00:00

abstract：:This paper studies the initial stages of iridescent tarnishes on titanium heat exchanger tubes in contact with running freshwater on the river Tagus in Spain for up to 20 months. Electrochemical impedance spectroscopy (EIS), scanning electron microscopy [(SEM with energy dispersive X-ray (EDX)] and X-ray photoelectron...

journal_title：Applied microbiology and biotechnology

authors： Moreno DA,Cano E,Ibars JR,Polo JL,Montero F,Bastidas JM

更新日期：2004-05-01 00:00:00

abstract：:Unravelling the mechanisms of how antibiotics influence growth performance through changes in gut microbiota can lead to the identification of highly productive microbiota in animal production. Here we investigated the effect of zinc bacitracin and avilamycin on growth performance and caecal microbiota in chickens and...

journal_title：Applied microbiology and biotechnology

authors： Crisol-Martínez E,Stanley D,Geier MS,Hughes RJ,Moore RJ

更新日期：2017-06-01 00:00:00

abstract：:Wastewater produced by the debittering process of green olives (GOW) is rich in polyphenolics and presents high chemical oxygen demand and alkalinity values. Eight white-rot fungi ( Abortiporus biennis, Dichomitus squalens, Inonotus hispidus, Irpex lacteus, Lentinus tigrinus, Panellus stipticus, Pleurotus ostreatus an...

journal_title：Applied microbiology and biotechnology

authors： Aggelis G,Ehaliotis C,Nerud F,Stoychev I,Lyberatos G,Zervakis GI

更新日期：2002-07-01 00:00:00

abstract：:Genetic engineering of transcription factors is an efficient strategy to improve lignocellulolytic enzyme production in fungi. In this study, the xylanase transcriptional regulators of Trichoderma reesei (Xyr1) and Neurospora crassa (XLR-1), as well as their constitutively active mutants (Xyr1A824V and XLR-1A828V), we...

journal_title：Applied microbiology and biotechnology

authors： Xia C,Li Z,Xu Y,Yang P,Gao L,Yan Q,Li S,Wang Y,Qu Y,Song X

更新日期：2019-03-01 00:00:00

abstract：:Both varied and strong promoters are essential for metabolic and pathway engineering applications in any host organism. To enable this capacity, here we demonstrate a generalizable method for the de novo construction of strong, synthetic hybrid promoter libraries. Specifically, we demonstrate how promoter truncation a...

journal_title：Applied microbiology and biotechnology

authors： Blazeck J,Reed B,Garg R,Gerstner R,Pan A,Agarwala V,Alper HS

更新日期：2013-04-01 00:00:00

abstract：:Nervonic acid (NA) is a major very long-chain monounsaturated fatty acid found in the white matter of mammalian brains, which plays a critical role in the treatment of psychotic disorders and neurological development. In the nature, NA has been synthesized by a handful plants, fungi, and microalgae. Although the metab...

journal_title：Applied microbiology and biotechnology

authors： Fan Y,Meng HM,Hu GR,Li FL

更新日期：2018-04-01 00:00:00

abstract：:The efficient fermentation of xylose can improve biofuel production. We previously developed a two-stage transcriptional reprogramming (TSTR) strategy (including a glucose fermentation stage and a xylose fermentation stage) and demonstrated its application for the construction of Saccharomyces cerevisiae strains with ...

journal_title：Applied microbiology and biotechnology

authors： Zhang X,Wang J,Zhang W,Hou JY,Xiao W,Cao L

更新日期：2018-08-01 00:00:00

abstract：:Molecular studies were performed to establish the causes of the superior lovastatin productivity of a novel solid-state fermentation (SSF) process, in relation with liquid submerged fermentation (SmF; 20 mg/g vs. 0.65 mg/ml). In SSF, biosynthetic genes lovE and lovF transcripts accumulated to high levels from day 1 to...

journal_title：Applied microbiology and biotechnology

authors： Barrios-González J,Baños JG,Covarrubias AA,Garay-Arroyo A

更新日期：2008-05-01 00:00:00

abstract：:Promoters of sorbose dehydrogenase gene sdh and sorbosone dehydrogenase gene sndh (Psdh and Psndh) in Ketogulonicigenium vulgare DSM 4025 were identified. The transcription initiation site (TIS) of Psdh was guanine 74 bp upstream of the start codon of sdh and the TIS of Psndh was adenine 113 bp upstream of the first c...

journal_title：Applied microbiology and biotechnology

authors： Fu S,Zhang W,Guo A,Wang J

更新日期：2007-07-01 00:00:00

abstract：:Plant health is determined by the comprehensive effect of soil physicochemical and biological properties. In this study, we compared the chemical properties and microbiomes of the rhizosphere soils of healthy, Fusarium oxysporum-infected, and dead watermelon plants and attempted to assess their potential roles in plan...

journal_title：Applied microbiology and biotechnology

authors： Meng T,Wang Q,Abbasi P,Ma Y

更新日期：2019-02-01 00:00:00

abstract：:Short interfering RNA (siRNA) is one of the members of the family of RNA interference (RNAi). Coupled with the RNA-induced silencing complex (RISC), siRNA is able to trigger the cleavage of target RNAs which serve as a defensive system against pathogens. Meanwhile, siRNA in gene silencing opens a new avenue for the tr...

journal_title：Applied microbiology and biotechnology

authors： Tam C,Wong JH,Cheung RCF,Zuo T,Ng TB

更新日期：2017-10-01 00:00:00

abstract：:The accumulation of toxic inorganic nitrogen is one of the major water quality problems in intensive aquaculture systems, thus the N removal in aquaculture systems is an important issue for the sustainable development of aquaculture. To understand one of the major microbial N removal processes, anaerobic ammonium oxid...

journal_title：Applied microbiology and biotechnology

authors： Li M,Gu JD

更新日期：2016-10-01 00:00:00

abstract：:Medicinal plants of the Dendrobium genus are highly prized, and hence, methodologies have been developed to authenticate Dendrobium drugs from its adulterants. Many bioactive constituents of Dendrobium species have been identified. The macromolecules included lectins; the enzymes chalcone synthase, sucrose synthase, a...

journal_title：Applied microbiology and biotechnology

authors： Ng TB,Liu J,Wong JH,Ye X,Wing Sze SC,Tong Y,Zhang KY

更新日期：2012-03-01 00:00:00

abstract：:Internal fragments of alpha- and beta-tubulin genes were generated using reverse transcription polymerase chain reaction (RT-PCR), and the termini were isolated using 5'- and 3'-rapid amplification of cDNA ends. Phytophthora capsici alpha- and beta-tubulin specific primers were then used to generate full-length cDNA b...

journal_title：Applied microbiology and biotechnology

authors： Koo BS,Park H,Kalme S,Park HY,Han JW,Yeo YS,Yoon SH,Kim SJ,Lee CM,Yoon MY

更新日期：2009-03-01 00:00:00

abstract：:Yeast is widely used in the baking, biocontrol, brewing, and bio manufacturing industries. In the baking industry alone, around two million tons of yeast are consumed worldwide every year. While yeast brings delicious and healthy lives to humans, we find that stress resistance of yeast is essential for the development...

journal_title：Applied microbiology and biotechnology

authors： Qiu X,Zhang J,Zhou J,Fang Z,Zhu Z,Li J,Du G

更新日期：2019-08-01 00:00:00

abstract：:Grasslands in semi-arid Northern China are widely desertified, thus inducing the formation of a large area of moving sand lands. Revegetation of the sandy land is commonly adopted to restore degraded grasslands. The structure of the soil microbial community might dramatically change during degradation and recovery bec...

journal_title：Applied microbiology and biotechnology

authors： Cao C,Zhang Y,Cui Z,Feng S,Wang T,Ren Q

更新日期：2017-08-01 00:00:00

abstract：:The efficient production of recombinant proteins such as antibodies typically involves the screening of an extravagant number of clones in order to finally select a stable and high-producing cell line. Thereby, the underlying principles of a powerful protein machinery, but also potential expression limitations, often ...

journal_title：Applied microbiology and biotechnology

authors： Reinhart D,Sommeregger W,Debreczeny M,Gludovacz E,Kunert R

更新日期：2014-07-01 00:00:00

abstract：:Plasmids without an origin of replication, but bearing the URA3 gene of Saccharomyces cerevisiae as a selective marker for transformation, are shown to replicate autonomously in Hansenula polymorpha, indicating that parts of the S. cerevisiae URA3 gene can fulfil an autonomous replication and stabilization function in...

journal_title：Applied microbiology and biotechnology

authors： Gatzke R,Weydemann U,Janowicz ZA,Hollenberg CP

更新日期：1995-10-01 00:00:00

abstract：:Clavulanic acid is a secondary metabolite produced by Streptomyces clavuligerus. It possesses a clavam structure and a characteristic 3R,5R stereochemistry essential for action as a beta-lactamase inhibitory molecule. It is produced from glyceraldehyde-3-phosphate and arginine in an eight step biosynthetic pathway. Th...

journal_title：Applied microbiology and biotechnology

authors： Liras P,Rodríguez-García A

更新日期：2000-10-01 00:00:00

abstract：:Dengue virus (DENV) infection is one of the most important public health threats globally; however, no vaccines or effective antivirals are currently available. The bioluminescence imaging technique has emerged as a powerful tool for studies on viral pathogenesis in vitro and in vivo. In this study, using a recombinan...

journal_title：Applied microbiology and biotechnology

authors： Li XF,Deng YQ,Zhao H,Ye Q,Wang HJ,Li SH,Zhu SY,Shi PY,Qin ED,Zhang B,Qin CF

更新日期：2013-05-01 00:00:00

abstract：:In Escherichia coli, the enzyme called cysteine desulfhydrase (CD), which is responsible for L-cysteine degradation, was investigated by native-PAGE and CD activity staining of crude cell extracts. Analyses with gene-disrupted mutants showed that CD activity resulted from two enzymes: tryptophanase (TNase) encoded by ...

journal_title：Applied microbiology and biotechnology

authors： Awano N,Wada M,Kohdoh A,Oikawa T,Takagi H,Nakamori S

更新日期：2003-08-01 00:00:00

abstract：:As the initial step in a project to provide a more cost-effective source of the phytase enzyme, this paper reports on the use of a polyclonal antibody raised to phytase purified from an isolate of Aspergillus niger (A. ficuum) to screen an A. niger lambda gt11 expression library and the use of amino acid sequencing to...

journal_title：Applied microbiology and biotechnology

authors： Mullaney EJ,Gibson DM,Ullah AH

更新日期：1991-08-01 00:00:00

abstract：:Biotin-dependent human acetyl-CoA carboxylases (ACCs) are integral in homeostatic lipid metabolism. By securing posttranslational biotinylation, ACCs perform coordinated catalytic functions allosterically regulated by phosphorylation/dephosphorylation and citrate. The production of authentic recombinant ACCs is heeded...

journal_title：Applied microbiology and biotechnology

authors： Hwang IW,Makishima Y,Kato T,Park S,Terzic A,Park EY

更新日期：2014-10-01 00:00:00

abstract：:Chimeric virus-like particles (VLPs) were developed as a candidate for allergen-specific immunotherapy. In this study, hepatitis B core antigen (HBcAg) that genetically fused to Chenopodium album polcalcin (Che a 3)-derived peptide was expressed in E. coli BL21, purified, and VLP formation was evaluated using native a...

journal_title：Applied microbiology and biotechnology

authors： Sani MZ,Bargahi A,Momenzadeh N,Dehghani P,Moghadam MV,Maleki SJ,Nabipour I,Shirkani A,Akhtari J,Hesamizadeh K,Heidari S,Omrani F,Akbarzadeh S,Mohammadi M

更新日期：2021-01-01 00:00:00

abstract：:Levulinic acid was esterified with methanol, ethanol, and 1-butanol with the final goal to predict the maximum yield of these equilibrium-limited reactions as function of medium composition. In a first step, standard reaction data (standard Gibbs energy of reaction Δ R g 0 ) were determined from experimental formation...

journal_title：Applied microbiology and biotechnology

authors： Altuntepe E,Emel'yanenko VN,Forster-Rotgers M,Sadowski G,Verevkin SP,Held C

更新日期：2017-10-01 00:00:00

abstract：:The formation and activity of an As(III)-oxidising biofilm in a bioreactor, using pozzolana as bacterial growth support, was studied for the purpose of optimising fixed-bed bioreactors for bioremediation. After 60 days of continuous functioning with an As(III)-contaminated effluent, the active biofilm was found to be ...

journal_title：Applied microbiology and biotechnology

authors： Michel C,Jean M,Coulon S,Dictor MC,Delorme F,Morin D,Garrido F

更新日期：2007-11-01 00:00:00

abstract：:Yeast artificial chromosomes (YAC) splitting technology was developed as a means to subclone any desired region of eukaryotic chromosomes from one YAC into new YACs. In the present study, the conventional YAC splitting technology was improved by incorporating PCR-mediated chromosome splitting technique and by adding a...

journal_title：Applied microbiology and biotechnology

authors： Kim Y,Sugiyama M,Yamagishi K,Kaneko Y,Fukui K,Kobayashi A,Harashima S

更新日期：2005-11-01 00:00:00