Positive identification of a lambda gt11 clone containing a region of fungal phytase gene by immunoprobe and sequence verification.

abstract：:Two putative chitinase genes, lin0153 and lin1996, from the nonpathogenic bacterium Listeria innocua were expressed in Escherichia coli, and the gene products were characterized. The genes were close homologs of chitinases from the pathogenic bacterium Listeria monocytogenes, in which chitinases and chitin-binding pro...

journal_title：Applied microbiology and biotechnology

authors： Honda S,Wakita S,Sugahara Y,Kawakita M,Oyama F,Sakaguchi M

更新日期：2016-09-01 00:00:00

abstract：:Xylose is, after glucose, the dominant sugar in agricultural wastes. In anaerobic environments, carbohydrates are converted into volatile fatty acids and alcohols. These can be used as building blocks in biotechnological or chemical processes, e.g., to produce bioplastics. In this study, xylose fermentation by mixed m...

journal_title：Applied microbiology and biotechnology

authors： Temudo MF,Mato T,Kleerebezem R,van Loosdrecht MC

更新日期：2009-02-01 00:00:00

abstract：:L-Malic acid and fumaric acid are C4 dicarboxylic organic acids and considered as promising chemical building blocks. They can be applied as food preservatives and acidulants in rust removal and as polymerization starter units. Molds of the genus Aspergillus are able to produce malic acid in large quantities from gluc...

journal_title：Applied microbiology and biotechnology

authors： Ochsenreither K,Fischer C,Neumann A,Syldatk C

更新日期：2014-06-01 00:00:00

abstract：:Protein translation, translocation, folding, processing, and secretion in eukaryotic cells are complex and not always straightforward processes, e.g., different routes of secretion and degradation exist. Formation of malfolded proteins in the endoplasmic reticulum (ER) can be one of the major bottlenecks for recombina...

journal_title：Applied microbiology and biotechnology

authors： Pfeffer M,Maurer M,Stadlmann J,Grass J,Delic M,Altmann F,Mattanovich D

更新日期：2012-03-01 00:00:00

abstract：:Extracellular manganese peroxidase (MnP) was purified from the compost extract of Agaricus bisporus using anion exchange chromatography, gel filtration and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Two forms (MnP1 and MnP2) were separated by isoelectric focusing and their isoelectric points...

journal_title：Applied microbiology and biotechnology

authors： Lankinen VP,Bonnen AM,Anton LH,Wood DA,Kalkkinen N,Hatakka A,Thurston CF

更新日期：2001-03-01 00:00:00

abstract：:The human granulocyte macrophage colony-stimulating factor (GM-CSF) is a glycoprotein with important clinical applications for the treatment of neutropenia and aplastic anemia and reducing infections associated with bone marrow transplants. We evaluated the potential for using a potato virus X (PVX) viral vector syste...

journal_title：Applied microbiology and biotechnology

authors： Zhou F,Wang ML,Albert HH,Moore PH,Zhu YJ

更新日期：2006-10-01 00:00:00

abstract：:Based on our previous study evaluating the in vivo cure efficacy of chitosan on bovine mastitis, a more water-soluble chitosan-oligosaccharide (OCHT) with a high degree of deacetylation and low molecular weight was prepared to obtain high antibiotic efficacy. The growth of Staphylococcus aureus isolated from bovine ma...

journal_title：Applied microbiology and biotechnology

authors： Moon JS,Kim HK,Koo HC,Joo YS,Nam HM,Park YH,Kang MI

更新日期：2007-07-01 00:00:00

abstract：:In this study, eight commercially available, chemically defined Chinese hamster ovary (CHO) cell culture media from different vendors were evaluated in batch culture using an IgG-producing CHO DG44 cell line as a model. Medium adaptation revealed that the occurrence of even small aggregates might be a good indicator o...

journal_title：Applied microbiology and biotechnology

authors： Reinhart D,Damjanovic L,Kaisermayer C,Kunert R

更新日期：2015-06-01 00:00:00

abstract：:In order to achieve efficient homo L-lactic acid fermentation from xylose, we first carried out addition of xylose assimilation ability to Lactococcus lactis IL 1403 by introducing a plasmid carrying the xylRAB genes from L. lactis IO-1 (pXylRAB). Then modification of xylose assimilation pathway was carried out. L. la...

journal_title：Applied microbiology and biotechnology

authors： Shinkawa S,Okano K,Yoshida S,Tanaka T,Ogino C,Fukuda H,Kondo A

更新日期：2011-09-01 00:00:00

abstract：:In order to enhance the removal efficiency of chemical oxygen demand (COD) in the high-salinity landfill leachate, the dominant strains were isolated from high-salinity landfill leachate. The dominant strains and bacteria consortium were screened for COD treatment potential using an aerobic COD concentration decrease ...

journal_title：Applied microbiology and biotechnology

authors： Guo L,Ji M,Dong H,Wei Y

更新日期：2009-08-01 00:00:00

abstract：:Brown rot wood-degrading fungi distinctly modify lignocellulose and completely hydrolyze polysaccharides (saccharification), typically without secreting an exo-acting glucanase and without removing lignin. Although each step of this two-step approach evolved within the same organism, it is unknown if the early lignoce...

journal_title：Applied microbiology and biotechnology

authors： Schilling JS,Tewalt JP,Duncan SM

更新日期：2009-09-01 00:00:00

abstract：:Ralstonia eutropha H850 was labelled chromosomally with a gfp marker gene encoding for the green fluorescent protein, and designated R. eutropha H850g13. Visual observation of green fluorescent cells under an epifluorescence microscope, and PCR amplification products, confirmed that the bacterium was labelled with gfp...

journal_title：Applied microbiology and biotechnology

authors： Abbey AM,Beaudette LA,Lee H,Trevors JT

更新日期：2003-12-01 00:00:00

abstract：:In a screening for proteolytically active lactic acid bacteria, three strains, Lactobacillus delbrueckii ssp. lactis 92202, Lactobacillus helveticus 92201, and Lactobacillus delbrueckii ssp. bulgaricus 92059, showed the highest activities following growth in milk. All three strains degraded α- and β-casein, but did no...

journal_title：Applied microbiology and biotechnology

authors： Kliche T,Li B,Bockelmann W,Habermann D,Klempt M,de Vrese M,Wutkowski A,Clawin-Raedecker I,Heller KJ

更新日期：2017-10-01 00:00:00

abstract：:Our previous work showed that there is a limitation in the use of dihydrofolate reductase (dhfr)/methotrexate (MTX)-mediated gene amplification systems in dhfr-non-deficient HEK293 cells, as endogenous dhfr may interfere with the amplification process. In the present study, we successfully generated Epstein-Barr virus...

journal_title：Applied microbiology and biotechnology

authors： Park SH,Park JH,Lee JH,Lee HM,Kang YJ,Lee EJ,Shin S,Lee GM,Kim YG

更新日期：2021-01-01 00:00:00

abstract：:Nanobiotechnology raises fascinating possibilities for new analytical assays in various fields such as bioelectronic assembly, biomechanics and sampling techniques, as well as in chips or micromachined devices. Recently, nanotechnology has greatly impacted biotechnological research with its potential applications in s...

journal_title：Applied microbiology and biotechnology

authors： Lee SJ,Lee SY

更新日期：2004-04-01 00:00:00

abstract：:Vaccination has been regarded as the most effective way to reduce death and morbidity caused by infectious diseases in the livestock industry. In this study, plasma activated water (PAW) was introduced to prepare the inactivated Newcastle disease vaccine. Humoral immune response was tested by hemagglutination inhibiti...

journal_title：Applied microbiology and biotechnology

authors： Hongzhuan Z,Ying T,Xia S,Jinsong G,Zhenhua Z,Beiyu J,Yanyan C,Lulu L,Jue Z,Bing Y,Jing F

更新日期：2020-01-01 00:00:00

abstract：:It has been well documented that Aureobasidium pullulans is widely distributed in different environments. Different strains of A. pullulans can produce amylase, proteinase, lipase, cellulase, xylanase, mannanase, transferases, pullulan, siderophore, and single-cell protein, and the genes encoding proteinase, lipase, c...

journal_title：Applied microbiology and biotechnology

authors： Chi Z,Wang F,Chi Z,Yue L,Liu G,Zhang T

更新日期：2009-04-01 00:00:00

abstract：:The construction of a new phagemid vector for display of peptides on the pVIII major coat protein of filamentous bacteriophage is described, in which expression of pVIII-peptide fusions was placed under the control of the arabinose-inducible P(BAD) promoter. The new phagemid showed excellent capacity for the regulatio...

journal_title：Applied microbiology and biotechnology

authors： Fagerlund A,Myrset AH,Kulseth MA

更新日期：2008-10-01 00:00:00

abstract：:Microbial transformation of benzaldehyde into L: -phenylacetylcarbinol by whole cell Saccharomyces cerevisiae has been carried out in a novel polyethylene glycol (PEG)-induced cloud point system. The system is composed of 80 g PEG 20,000, 75 ml Triton X-100, 20 g peptone, 10 g yeast extract, 25 g glucose, 1 g MgSO(4)....

journal_title：Applied microbiology and biotechnology

authors： Zhang W,Wang Z,Li W,Zhuang B,Qi H

更新日期：2008-02-01 00:00:00

abstract：:L-Ribose is a rare and expensive sugar that can be used as a precursor for the production of L-nucleoside analogues, which are used as antiviral drugs. In this work, we describe a novel way of producing L-ribose from the readily available raw material L-arabinose. This was achieved by introducing L-ribose isomerase ac...

journal_title：Applied microbiology and biotechnology

authors： Helanto M,Kiviharju K,Granström T,Leisola M,Nyyssölä A

更新日期：2009-05-01 00:00:00

abstract：:In a previous study, a marine isolate Clostridium sp. EDB2 degraded 2,4,6,8,10,12-hexanitro-2,4,6,8,10,12-hexaazaisowurtzitane (CL-20) under anaerobic conditions (Bhushan B, Halasz A, Thiboutot S, Ampleman G, Hawari J (2004c) Chemotaxis-mediated biodegradation of cyclic nitramine explosives RDX, HMX, and CL-20 by Clos...

journal_title：Applied microbiology and biotechnology

authors： Bhushan B,Halasz A,Hawari J

更新日期：2005-12-01 00:00:00

abstract：:A purification procedure for an extracellular alpha-amylase from Streptomyces rimosus, oxytetracycline-producing strain, is described. The enzyme obtained was shown to be an acidic (pI 4.75) monomer with a relative molecular mass (M(r)) of 43,000, containing three cysteines involved in the catalytic activity of the en...

journal_title：Applied microbiology and biotechnology

authors： Vukelić B,Ritonja A,Renko M,Pokorny M,Vitale L

更新日期：1992-05-01 00:00:00

abstract：:The polyene macrolide rimocidin, produced by Streptomyces rimosus M527, is highly effective against a broad range of fungal plant pathogens, but at low yields. Elicitation is an effective method of stimulating the yield of bioactive secondary metabolites. In this study, the biomass and filtrate of a culture broth of E...

journal_title：Applied microbiology and biotechnology

authors： Song Z,Ma Z,Bechthold A,Yu X

更新日期：2020-05-01 00:00:00

abstract：:A batch fermentation strategy using Xanthomonas campestris ATCC 13951 for xanthan gum production has been established in which all essential medium components are supplied at the onset. This has been achieved using sucrose as sole sugar feedstock. Sequential consumption of nitrogen sources (soybean hydrolysates, ammon...

journal_title：Applied microbiology and biotechnology

authors： Letisse F,Chevallereau P,Simon JL,Lindley ND

更新日期：2001-05-01 00:00:00

abstract：:Biodegradation can achieve complete and cost-effective elimination of aromatic pollutants through harnessing diverse microbial metabolic processes. Aromatics biodegradation plays an important role in environmental cleanup and has been extensively studied since the inception of biodegradation. These studies, however, a...

journal_title：Applied microbiology and biotechnology

authors： Cao B,Nagarajan K,Loh KC

更新日期：2009-11-01 00:00:00

abstract：:Biotechnologies that aim to produce renewable fuels, chemicals, and bioproducts from residual ligno(hemi)cellulosic biomass mostly rely on enzymatic depolymerization of plant cell walls (PCW). This process requires an arsenal of diverse enzymes, including xylanases, which synergistically act on the hemicellulose, redu...

journal_title：Applied microbiology and biotechnology

authors： Evangelista DE,de Oliveira Arnoldi Pellegrini V,Santo ME,McQueen-Mason S,Bruce NC,Polikarpov I

更新日期：2019-10-01 00:00:00

abstract：:This study is an application of an experimental design methodology for the optimization of the culture conditions of alpha-L: -arabinofuranosidase production by Talaromyces thermophilus. Wheat bran and yeast extract were first selected as the best carbon and nitrogen sources, respectively, for enzyme production. A Pla...

journal_title：Applied microbiology and biotechnology

authors： Guerfali M,Chaabouni M,Gargouri A,Belghith H

更新日期：2010-02-01 00:00:00

abstract：:Multidrug-resistant Acinetobacter baumannii is a well-documented pathogen associated with hospital-acquired infections. In addition to multidrug resistance, A. baumannii can also become resistant to colistin, the antibiotic treatment of last resort, by the loss of the lipopolysaccharide from its outer membrane. Here, ...

journal_title：Applied microbiology and biotechnology

authors： Tseng CC,Tsai YH,Hu A,Liou JW,Chang KC,Chang HH

更新日期：2016-10-01 00:00:00

abstract：:Ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) contribute significantly to the nitrogen cycle. The community structure of AOA and AOB is diverse in the different wetlands. Few studies have comparatively investigated the community structure and dynamics of ammonia-oxidizing organisms in the marsh ...

journal_title：Applied microbiology and biotechnology

authors： Gao D,Liu F,Xie Y,Liang H

更新日期：2018-08-01 00:00:00

abstract：:Polyunsaturated fatty acids, docosahexaenoic acid (DHA, 22:6, n-3), eicosapentaenoic acid (EPA, 20:5, n-3), and arachidonic acid (ARA, 20:4 n-6), have multiple beneficial effects on human health and can be used as an important ingredient in dietary supplements, food, feed and pharmaceuticals. A variety of microorganis...

journal_title：Applied microbiology and biotechnology

authors： Wu L,Roe CL,Wen Z

更新日期：2013-09-01 00:00:00