Abstract:
:Microbial transformation of benzaldehyde into L: -phenylacetylcarbinol by whole cell Saccharomyces cerevisiae has been carried out in a novel polyethylene glycol (PEG)-induced cloud point system. The system is composed of 80 g PEG 20,000, 75 ml Triton X-100, 20 g peptone, 10 g yeast extract, 25 g glucose, 1 g MgSO(4).7H(2)O, 0.05 g CaCl(2).2H(2)O, 35 g Na(2)HPO(4).12H(2)O, and 10.7 g citric acid per liter of tap water. The microbial transformation is conducted at 0.6 ml of acetaldehyde (35% volume content), 0.9 ml of benzaldehyde, and 7 g of wet cell per 100 ml of the PEG-induced cloud point system. Under the conditions, a relatively longer-term bioactivity of whole cell microorganism in the PEG-induced cloud point system has been achieved. A fed-batch microbial transformation process with a discrete addition of glucose and substrate gets a high final product concentration of about 8 g/l.
journal_name
Appl Microbiol Biotechnoljournal_title
Applied microbiology and biotechnologyauthors
Zhang W,Wang Z,Li W,Zhuang B,Qi Hdoi
10.1007/s00253-007-1304-2subject
Has Abstractpub_date
2008-02-01 00:00:00pages
233-9issue
2eissn
0175-7598issn
1432-0614journal_volume
78pub_type
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