Impact of overproduced heterologous protein characteristics on physiological response in Yarrowia lipolytica steady-state-maintained continuous cultures.

abstract：:Extremely acidophilic microorganisms have an optimal pH of <3 and are found in all three domains of life. As metals are more soluble at acid pH, acidophiles are often challenged by very high metal concentrations. Acidophiles are metal-tolerant by both intrinsic, passive mechanisms as well as active systems. Passive me...

journal_title：Applied microbiology and biotechnology

authors： Dopson M,Holmes DS

更新日期：2014-10-01 00:00:00

abstract：:Cyanophycin is non-ribosomally synthesized protein-like copolymer. Synthesis of cyanophycin is catalyzed by cyanophycin synthetase (CphA). In this study, a novel cyanophycin synthetase CphA49 belonging to NOR5 clade of Gammaproteobacteria was identified with primer-based screening from a deep-sea sediment metagenomic ...

journal_title：Applied microbiology and biotechnology

authors： Du J,Li L,Ding X,Hu H,Lu Y,Zhou S

更新日期：2013-10-01 00:00:00

abstract：:A cDNA fragment encoding the A catalytic domain of the Neocallimastix frontalis endoxylanase XYN3 was amplified and cloned by the polymerase chain reaction technique. The xyn3A DNA fragment was inserted between the Saccharomyces cerevisiae phosphoglycerate kinase gene promoter and terminator sequences on a multicopy e...

journal_title：Applied microbiology and biotechnology

authors： Durand R,Rascle C,Fèvre M

更新日期：1999-08-01 00:00:00

abstract：:Cheese whey (CW), the liquid resulting from the precipitation and removal of milk casein during cheese-making, and the second cheese whey (SCW) derived from the production of cottage and ricotta cheeses are the main byproducts of dairy industry. The major constituent of CW and SCW is lactose, contributing to the high ...

journal_title：Applied microbiology and biotechnology

authors： Zotta T,Solieri L,Iacumin L,Picozzi C,Gullo M

更新日期：2020-04-01 00:00:00

abstract：:We have constructed a chimeric protein composed of the receptor binding and membrane translocation domains of Pseudomonas exotoxin A (PE) with the outer membrane proteins I and F, together designated as PEIF. The potential of PEIF as a vaccine against Pseudomonas infection was evaluated in BALB/c mice and New Zealand ...

journal_title：Applied microbiology and biotechnology

authors： Chen TY,Shang HF,Chen TL,Lin CP,Hui CF,Hwang J

更新日期：1999-10-01 00:00:00

abstract：:Lactic acid bacteria are extensively used in food technology and for the production of various compounds, but they are fastidious in nutrient requirements. In order to elucidate the role of each component precisely, defined multicomponent media are required. This study focuses on determining nutrient auxotrophies and ...

journal_title：Applied microbiology and biotechnology

authors： Aller K,Adamberg K,Timarova V,Seiman A,Feštšenko D,Vilu R

更新日期：2014-07-01 00:00:00

abstract：INTRODUCTION:Hitherto the production of the biopolymer cyanophycin (CGP) using recombinant Escherichia coli strains and cheap mineral salts medium yielded only trace amounts of CGP (<0.5%, w/w) of the cell dry matter (CDM). This was probably due to the instability of the plasmids encoding the cyanophycin synthetase. M...

journal_title：Applied microbiology and biotechnology

authors： Kroll J,Klinter S,Steinbüchel A

更新日期：2011-02-01 00:00:00

abstract：:A newly isolated strain of Cunninghamella echinulata grown on glucose produced significant quantities of biomass and cellular lipids in media with high C/N ratio. The oil yield from glucose consumed increased after nitrogen exhaustion in the growth medium, but gamma-linolenic acid (GLA) content in cellular oil systema...

journal_title：Applied microbiology and biotechnology

authors： Gema H,Kavadia A,Dimou D,Tsagou V,Komaitis M,Aggelis G

更新日期：2002-03-01 00:00:00

abstract：:The sustainable and practical degradation of intact chicken feathers by a newly isolated thermophilic bacterium Meiothermus ruber H328 was presented with extensive data. Aerobic cultivation with moderately thermophilic strain H328 at 55 degrees C for 6 days led to the apparently complete decay of the truly intact feat...

journal_title：Applied microbiology and biotechnology

authors： Matsui T,Yamada Y,Mitsuya H,Shigeri Y,Yoshida Y,Saito Y,Matsui H,Watanabe K

更新日期：2009-04-01 00:00:00

abstract：:A microbial peptide amidase was found in a limited screening and purified about 500-fold from Stenotrophomonas maltophilia. The native enzyme has a molecular mass of 38 kDa (gel filtration). The sequence of the first 16 amino acids was determined by Edman degradation. The isoelectric point was found to be around 5.8. ...

journal_title：Applied microbiology and biotechnology

authors： Stelkes-Ritter U,Wyzgol K,Kula MR

更新日期：1995-12-01 00:00:00

abstract：:Biotin-dependent human acetyl-CoA carboxylases (ACCs) are integral in homeostatic lipid metabolism. By securing posttranslational biotinylation, ACCs perform coordinated catalytic functions allosterically regulated by phosphorylation/dephosphorylation and citrate. The production of authentic recombinant ACCs is heeded...

journal_title：Applied microbiology and biotechnology

authors： Hwang IW,Makishima Y,Kato T,Park S,Terzic A,Park EY

更新日期：2014-10-01 00:00:00

abstract：:Toluene dioxygenase (TDO) catalyzes asymmetric cis-dihydroxylation of aromatic compounds. To achieve high efficient biotransformation of benzene to benzene cis-diols, Pseudomonas putida KT2442, Pseudomonas stutzeri 1317, and Aeromonas hydrophila 4AK4 were used as hosts to express TDO gene tod. Plasmid pSPM01, a deriva...

journal_title：Applied microbiology and biotechnology

authors： Ouyang SP,Sun SY,Liu Q,Chen J,Chen GQ

更新日期：2007-02-01 00:00:00

abstract：:Phytoplankton blooms are predictable features of marine and freshwater habitats. Despite a good knowledge base of the environmental factors controlling blooms, complex interactions between the bacterial and archaeal communities and phytoplankton bloom taxa are only now emerging. Here, the current research on bacterial...

journal_title：Applied microbiology and biotechnology

authors： Song L

更新日期：2017-06-01 00:00:00

abstract：:Sulfide detoxification can be catalyzed by ancient membrane-bound flavoproteins, sulfide:quinone oxidoreductases (Sqr), which have important roles in sulfide homeostasis and sulfide-dependent energy conservation processes by transferring electrons from sulfide to respiratory or photosynthetic membrane electron flow. S...

journal_title：Applied microbiology and biotechnology

authors： Duzs Á,Tóth A,Németh B,Balogh T,Kós PB,Rákhely G

更新日期：2018-06-01 00:00:00

abstract：:Brown rot wood-degrading fungi distinctly modify lignocellulose and completely hydrolyze polysaccharides (saccharification), typically without secreting an exo-acting glucanase and without removing lignin. Although each step of this two-step approach evolved within the same organism, it is unknown if the early lignoce...

journal_title：Applied microbiology and biotechnology

authors： Schilling JS,Tewalt JP,Duncan SM

更新日期：2009-09-01 00:00:00

abstract：:Mitogen-activated protein kinase (MAPK) cascades are broadly conserved and play essential roles in multiple cellular processes, including fungal development, pathogenicity, and secondary metabolism. Their function, however, also exhibits species and strain specificity. Penicillium oxalicum secretes plant-biomass-degra...

journal_title：Applied microbiology and biotechnology

authors： Ma B,Ning YN,Li CX,Tian D,Guo H,Pang XM,Luo XM,Zhao S,Feng JX

更新日期：2021-01-01 00:00:00

abstract：:Omega-3 fatty acids (ω3 FAs) have attracted attention because they have various health benefits for humans. Fish oils are currently major sources of ω3 FAs, but a sustainable supply of ω3 FAs based on fish oils is problematic because of the increasing demand. In this study, the production potential of a genetically en...

journal_title：Applied microbiology and biotechnology

authors： Yoshino T,Kakunaka N,Liang Y,Ito Y,Maeda Y,Nomaguchi T,Matsunaga T,Tanaka T

更新日期：2017-09-01 00:00:00

abstract：:The microbes present in bioethanol production processes have been previously studied in laboratory-scale experiments, but there is a lack of information on full-scale industrial processes. In this study, the microbial communities of three industrial bioethanol production processes were characterized using several meth...

journal_title：Applied microbiology and biotechnology

authors： Aho VTE,Tolonen T,Haverinen J,Jaakkola M,Paulin L,Auvinen P,Laine MM

更新日期：2020-09-01 00:00:00

abstract：:Extracellular matrix provides an architectural structure and mechanical stability for aerobic granules. Distributions of cells and extracellular polymeric substances (EPS), including proteins, alpha- and beta-D-glucopyranose polysaccharides, in acetate-fed granules and phenol-fed granules were probed using a novel qua...

journal_title：Applied microbiology and biotechnology

authors： Chen MY,Lee DJ,Tay JH

更新日期：2007-01-01 00:00:00

abstract：:Dengue viruses are emerging mosquito-borne pathogens belonging to Flaviviridae family which are transmitted to humans via the bites of infected mosquitoes Aedes aegypti and Aedes albopictus. Because of the wide distribution of these mosquito vectors, more than 2.5 billion people are approximately at risk of dengue inf...

journal_title：Applied microbiology and biotechnology

authors： Fahimi H,Mohammadipour M,Haddad Kashani H,Parvini F,Sadeghizadeh M

更新日期：2018-04-01 00:00:00

abstract：:Cells of an aerobic three-membered bacterial co-culture, designated as ECO3, capable of cometabolizing and aerobically dechlorinating low-chlorinated biphenyls in the presence of biphenyl, were immobilized on Manville silica beads, on frosted-glass beads and on polyurethane foam cubes in packed-bed bioreactors continu...

journal_title：Applied microbiology and biotechnology

authors： Fava F,Di Gioia D,Marchetti L,Quattroni G

更新日期：1996-05-01 00:00:00

abstract：:Understanding the effect of conserved domains reconstruction of alginate lyases on action mode is essential for their application and in-depth study. We report the expression and action mode of recombinant alginate lyase (AlyM) and its conserved domain reconstruction forms (AlyMΔCBM, cAlyM, and AlyMΔ58C). The enzymati...

journal_title：Applied microbiology and biotechnology

authors： Yang M,Li N,Yang S,Yu Y,Han Z,Li L,Mou H

更新日期：2019-01-01 00:00:00

abstract：:Bovine mastitis results in billion dollar losses annually in the USA alone. Streptococci are among the most relevant causative agents of this disease. Conventional antibiotic therapy is often unsuccessful and contributes to development of antibiotic resistance. Bacteriophage endolysins represent a new class of antimic...

journal_title：Applied microbiology and biotechnology

authors： Schmelcher M,Powell AM,Camp MJ,Pohl CS,Donovan DM

更新日期：2015-10-01 00:00:00

abstract：:Pyruvate carboxylase is an important anaplerotic enzyme replenishing oxaloacetate consumed for biosynthesis during growth, or lysine and glutamic acid production in industrial fermentations. We used regions of homology from pyruvate carboxylase sequences of 12 different species (corresponding to the ATP- and pyruvate-...

journal_title：Applied microbiology and biotechnology

authors： Koffas MA,Ramamoorthi R,Pine WA,Sinskey AJ,Stephanopoulos G

更新日期：1998-09-01 00:00:00

abstract：:A system for the controlled expression of a foreign gene in cultured tobacco cells (Nicotiana tabacum, BY2) by temperature shift was constructed. A 925-base-pair (bp) DNA fragment containing the 5'-flanking region of a low-molecular-mass heat-shock protein gene (HSP18.2) of Arabidopsis thaliana was inserted upstream o...

journal_title：Applied microbiology and biotechnology

authors： Yoshida K,Kasai T,Garcia MR,Sawada S,Shoji T,Shimizu S,Yamazaki K,Komeda Y,Shinmyo A

更新日期：1995-12-01 00:00:00

abstract：:Cyanobacterial blooms have become a serious problem in Lake Taihu during the last 20 years, and Microcystis aeruginosa and Synechococcus sp. are the two dominant species in cyanobacterial blooms of Lake Taihu. A freshwater bacterial strain, Shewanella sp. Lzh-2, with strong algicidal properties against harmful cyanoba...

journal_title：Applied microbiology and biotechnology

authors： Li Z,Lin S,Liu X,Tan J,Pan J,Yang H

更新日期：2014-05-01 00:00:00

abstract：:The ability of acetic acid bacteria (AAB) to produce cellulose has gained much industrial interest due to the physical and chemical characteristics of bacterial cellulose. The production of cellulose occurs in the presence of oxygen and in a glucose-containing medium, but it can also occur during vinegar elaboration b...

journal_title：Applied microbiology and biotechnology

authors： Valera MJ,Torija MJ,Mas A,Mateo E

更新日期：2015-02-01 00:00:00

abstract：:The genome of the coprophilous fungus Podospora anserina displays an impressive array of genes encoding hemicellulolytic enzymes. In this study, we focused on a putative carbohydrate esterase (CE) from family 16 (CE16) that bears a carbohydrate-binding module from family CBM1. The protein was heterologously expressed ...

journal_title：Applied microbiology and biotechnology

authors： Puchart V,Berrin JG,Haon M,Biely P

更新日期：2015-12-01 00:00:00

abstract：:Polyhydroxybutyrate (PHB) is an attractive substitute for petrochemical plastic due to its similar properties, biocompatibility, and biodegradability. The cost of scaled-up PHB production inhibits its widespread usage. Intensive researches are growing to reduce costs and improve thermomechanical, physical, and process...

journal_title：Applied microbiology and biotechnology

authors： Khosravi-Darani K,Mokhtari ZB,Amai T,Tanaka K

更新日期：2013-02-01 00:00:00

abstract：:This paper reports the in vivo expression of the synthetic transposase gene tnp(a) from a hyperactive Tn5 tnp gene mutant in Streptomyces coelicolor. Using the synthetic tnp(a) gene adapted for Streptomyces codon usage, we showed random insertion of the transposon into the Streptomycetes genome. The insertion frequenc...

journal_title：Applied microbiology and biotechnology

authors： Petzke L,Luzhetskyy A

更新日期：2009-07-01 00:00:00