Distribution of extracellular polymeric substances in aerobic granules.

abstract：:Polyketides, such as erythromycin, are complex natural products with diverse therapeutic applications. They are synthesized by multi-modular megaenzymes, so-called polyketide synthases (PKSs). The macrolide core of erythromycin, 6-deoxyerythronolide B (6dEB), is produced by the deoxyerythronolide B synthase (DEBS) tha...

journal_title：Applied microbiology and biotechnology

authors： Kumpfmüller J,Methling K,Fang L,Pfeifer BA,Lalk M,Schweder T

更新日期：2016-02-01 00:00:00

abstract：:Repetitious gene cassettes that encode the consensus decapeptide repeat of Mytilus edulis bioadhesive protein were designed, constructed, and expressed in Escherichia coli. The bioadhesive precursor (BP) with a relative molecular mass of 25,000 was expressed from one 600-bp gene at levels approaching 60% of total cell...

journal_title：Applied microbiology and biotechnology

authors： Salerno AJ,Goldberg I

更新日期：1993-05-01 00:00:00

abstract：:Zearalenone (ZEN) is one of the common mycotoxins with quite high occurrence rate and is harmful to animal and human health. Lactobacillus reuteri is known as a probiotic bacterium with active immune stimulating and high inhibitory activity against pathogenic microorganisms. In this study, we expressed the lactonohydr...

journal_title：Applied microbiology and biotechnology

authors： Liu F,Malaphan W,Xing F,Yu B

更新日期：2019-11-01 00:00:00

abstract：:In this study, we applied a 16S ribosomal RNA (rRNA) metagenomics approach to survey inanimate hospital environments (IHEs) in a respiratory care center (RCC). A total of 16 samples, including 9 from medical devices and 7 from workstations, were analyzed. Besides, clinical isolates were retrospectively analyzed during...

journal_title：Applied microbiology and biotechnology

authors： Tang CY,Yiu SM,Kuo HY,Tan TS,Liao KH,Liu CC,Hon WK,Liou ML

更新日期：2015-03-01 00:00:00

abstract：:Pseudomonas resinovorans, a known medium-chain-length (mcl-) poly(hydroxyalkanoate) (PHA) producer, was grown on 13-methyltetradecanoic acid (13-MTDA) and a mixture of isostearic acid (IA) isomers to produce methyl-branched mcl-PHA polymers. Shake-flask experiments revealed polymer productivities (the percent of the c...

journal_title：Applied microbiology and biotechnology

authors： Ashby RD,Ngo HL,Solaiman DK,Strahan G

更新日期：2009-11-01 00:00:00

abstract：:Rhodococcus erythropolis N9T-4, which is an extremely oligotrophic bacterium, can survive in a completely inorganic medium with no additional carbon source. This bacterium utilizes atmospheric CO2, but does not require any additional energy source such as light and hydrogen gas, required by autotrophic microorganisms....

journal_title：Applied microbiology and biotechnology

authors： Matsuoka T,Yoshida N

更新日期：2019-05-01 00:00:00

abstract：:Microbes engineered to display heterologous proteins could be useful biotechnological tools for protein engineering, lignocellulose degradation, biocatalysis, bioremediation, and biosensing. Bacillus subtilis is a promising host to display proteins, as this model Gram-positive bacterium is genetically tractable and al...

journal_title：Applied microbiology and biotechnology

authors： Huang GL,Gosschalk JE,Kim YS,Ogorzalek Loo RR,Clubb RT

更新日期：2018-08-01 00:00:00

abstract：:Since 2015, outbreaks of hepatitis-hydropericardium syndrome (HPS) caused by a novel genotype of fowl adenovirus 4 (FAdV-4) infection have created serious economic losses in China. Given that other serotypes of hypervirulent FAdVs have also been reported in poultry around the world, a common ELISA for all serotypes wi...

journal_title：Applied microbiology and biotechnology

authors： Pan Q,Wang J,Gao Y,Cui H,Liu C,Qi X,Zhang Y,Wang Y,Li K,Gao L,Wang X

更新日期：2020-01-01 00:00:00

abstract：:This review addresses metal uptake specifically by yeast. Metal uptake may be passive, active or both, depending on the viability of the biomass, and is influenced by a number of environmental and experimental factors. Uptake is typically accompanied by a degree of ion exchange and, under certain conditions, may be en...

journal_title：Applied microbiology and biotechnology

authors： Blackwell KJ,Singleton I,Tobin JM

更新日期：1995-08-01 00:00:00

abstract：:Leavening ability in sweet dough is required for the commercial applications of baker's yeast. This property depends on many factors, such as glycolytic activity, sucrase activity, and osmotolerance. This study explored the importance of sucrase level on the leavening ability of baker's yeast in sweet dough. Furthermo...

journal_title：Applied microbiology and biotechnology

authors： Zhang CY,Lin X,Feng B,Liu XE,Bai XW,Xu J,Pi L,Xiao DG

更新日期：2016-07-01 00:00:00

abstract：:Although a large number of AroA enzymes (5-enopyruvylshikimate-3-phosphate synthase [EPSPS]) have been identified, cloned and tested for glyphosate resistance, only AroA variants derived from Agrobacterium tumefaciens strain CP4 have been successfully used commercially. We have now used a polymerase chain reaction (PC...

journal_title：Applied microbiology and biotechnology

authors： Tian YS,Xu J,Xiong AS,Zhao W,Gao F,Fu XY,Peng RH,Yao QH

更新日期：2012-01-01 00:00:00

abstract：:Enantioselective oxidation of racemic phenyl-1,2-ethanediol into (R)-(-)-mandelic acid by a newly isolated Brevibacterium lutescens CCZU12-1 was demonstrated. It was found that optically active (R)-(-)-mandelic acid (e.e.p > 99.9 %) is produced leaving the other enantiomer (S)-(+)-phenyl-1,2-ethanediol intact. Using f...

journal_title：Applied microbiology and biotechnology

authors： He YC,Ma CL,Zhang X,Li L,Xu JH,Wu MX

更新日期：2013-08-01 00:00:00

abstract：:Glucose 6-phosphate dehydrogenase (G6PDH) is one of the most important dehydrogenases responsible for generating reduced NADPH for anabolism and is also the rate-limiting enzyme in the Entner-Doudoroff pathway. For in vitro biocatalysis, G6PDH must possess both high activity and good thermostability due to requirement...

journal_title：Applied microbiology and biotechnology

authors： Huang R,Chen H,Zhou W,Ma C,Zhang YP

更新日期：2018-04-01 00:00:00

abstract：:Human epidermal growth factor receptors (EGFR) are an important target in drug discovery in terms of both protein-small-molecule interactions and protein-protein interactions. In this work, the isolation of a stable soluble protein of the tyrosine kinase domain of EGFR in Escherichia coli expression has been accomplis...

journal_title：Applied microbiology and biotechnology

authors： Seetaha S,Ratanabanyong S,Choowongkomon K

更新日期：2019-10-01 00:00:00

abstract：:Streptokinase, a therapeutically important thrombolytic agent, is prone to C-terminal degradation and plasmin-mediated proteolytic processing. Since the protein was glycosylated during secretion from Pichia pastoris, therefore, the role of carbohydrate moieties on its stability was analyzed via in vivo blocking of N-g...

journal_title：Applied microbiology and biotechnology

authors： Adivitiya,Babbal,Mohanty S,Khasa YP

更新日期：2018-12-01 00:00:00

abstract：:A strong promoter increases transcription of the genes of interest and enhances the production of various valuable substances. For a hyperthermophilic archaeon Thermococcus onnurineus NA1, which can produce H2 from carbon monoxide oxidation, we searched for a novel endogenous strong promoter by transcriptome analysis ...

journal_title：Applied microbiology and biotechnology

authors： Lee SH,Kim MS,Jung HC,Lee J,Lee JH,Lee HS,Kang SG

更新日期：2015-05-01 00:00:00

abstract：:Plants as well as pro- and eukaryotic microorganisms are able to synthesise lysine via de novo synthesis. While plants and bacteria, with some exceptions, rely on variations of the meso-diaminopimelate pathway for lysine biosynthesis, fungi exclusively use the α-aminoadipate pathway. Although bacteria and fungi are, i...

journal_title：Applied microbiology and biotechnology

authors： Fazius F,Zaehle C,Brock M

更新日期：2013-05-01 00:00:00

abstract：:Poly(hydroxyalkanoates) (PHAs) constitute biodegradable polyesters and are considered among the most promising candidates to replace common petrochemical plastics in various applications. To date, all commercial processes for PHA production employ microbial discontinuous fed-batch fermentations. These processes featur...

journal_title：Applied microbiology and biotechnology

authors： Atlić A,Koller M,Scherzer D,Kutschera C,Grillo-Fernandes E,Horvat P,Chiellini E,Braunegg G

更新日期：2011-07-01 00:00:00

abstract：:A gratuitous strain was developed by disrupting the GAL1 gene (galactokinase) of recombinant Saccharomyces cerevisiae harboring the antithrombotic hirudin gene in the chromosome under the control of the GAL10 promoter. A series of glucose-limited fed-batch cultures were carried out to examine the effects of glucose su...

journal_title：Applied microbiology and biotechnology

authors： Kim MD,Lee TH,Lim HK,Seo JH

更新日期：2004-08-01 00:00:00

abstract：:The microbial communities of plant ecosystems are in relation to plant growing environment, but the alteration in biodiversity of rhizosphere and phyllosphere microbial communities in closed and controlled environments is unknown. The purpose of this study is to analyze the change regularity of microbial communities w...

journal_title：Applied microbiology and biotechnology

authors： Qin Y,Fu Y,Dong C,Jia N,Liu H

更新日期：2016-05-01 00:00:00

abstract：:Trichoderma harzianum is a widely distributed soil fungus that antagonizes numerous fungal phytopathogens. The antagonism of T. harzianum usually correlates with the production of antifungal activities including the secretion of fungal cell walls that degrade enzymes such as chitinases. Chitinases Chit42 and Chit33 fr...

journal_title：Applied microbiology and biotechnology

authors： Limón MC,Chacón MR,Mejías R,Delgado-Jarana J,Rincón AM,Codón AC,Benítez T

更新日期：2004-06-01 00:00:00

abstract：:Using organically synthesized hydroxyalkanoate coenzyme A thioesters, the activities of two short-chain polyhydroxalkanoate (PHA) synthases were investigated--Ralstonia eutropha PHA synthase (a type I PHA synthase) and Ectothiorhodospira shaposhnikovii PHA synthase (a type III synthase). The results indicate that the ...

journal_title：Applied microbiology and biotechnology

authors： Zhang S,Kamachi M,Takagi Y,Lenz RW,Goodwin S

更新日期：2001-07-01 00:00:00

abstract：:The 3D structures of Fim H and PapG proteins complexed with the host carbohydrate receptor demonstrate that both utilize binding-pocket asparagines for contact or stabilization with the carbohydrate. Pretreatment of whole bacteria with asparaginase resulted in decreased fimbriae-mediated attachment to urinary epitheli...

journal_title：Applied microbiology and biotechnology

authors： Wehner MR,Koch SR,Kindinger L,Nelson N,Cowan MM

更新日期：2004-11-01 00:00:00

abstract：:Polygalacturonases (PG) have evolved in the past years from a pectinase "simply" being used for food processing to an important parameter in plant-fungal interaction. PG-inhibiting proteins (PGIP) that are synthesised in plants as a specific response to PGs of pathogenic fungi, have become a focus as a possible target...

journal_title：Applied microbiology and biotechnology

authors： Lang C,Dörnenburg H

更新日期：2000-04-01 00:00:00

abstract：:During the genomics era, the use of thermostable DNA polymerases increased greatly. Many were identified and described-mainly of the genera Thermus, Thermococcus and Pyrococcus. Each polymerase has different features, resulting from origin and genetic modification. However, the rational choice of the adequate polymera...

journal_title：Applied microbiology and biotechnology

authors： Terpe K

更新日期：2013-12-01 00:00:00

abstract：:Ferulic acid was efficiently released from a wheat bran preparation by a ferulic acid esterase from Aspergillus niger (FAE-III) when incubated together with a Trichoderma viride xylanase (a maximum of 95% total ferulic acid released after 5 h incubation). FAE-III by itself could release ferulic acid but at a level alm...

journal_title：Applied microbiology and biotechnology

authors： Faulds CB,Williamson G

更新日期：1995-11-01 00:00:00

abstract：:Wild-type Corynebacterium glutamicum produced 0.6 g l(-1) xylitol from xylose at a productivity of 0.01 g l(-1) h(-1) under oxygen deprivation. To increase this productivity, the pentose transporter gene (araE) from C. glutamicum ATCC31831 was integrated into the C. glutamicum R chromosome. Consequent disruption of it...

journal_title：Applied microbiology and biotechnology

authors： Sasaki M,Jojima T,Inui M,Yukawa H

更新日期：2010-04-01 00:00:00

abstract：:Edwardsiella tarda is a leading fish pathogen haunting worldwide aquaculture industry. In E. tarda, two-component system EsrA-EsrB positively regulates type III and VI secretion systems (T3SS and T6SS) and negatively regulates hemolysin EthA, which has been demonstrated to be essential for the invasion processes in fi...

journal_title：Applied microbiology and biotechnology

authors： Yang W,Wang L,Zhang L,Qu J,Wang Q,Zhang Y

更新日期：2015-02-01 00:00:00

abstract：:Inactivation of D-amino acid oxidase occurred by different mechanisms. The enzyme showed a rapid loss of activity in the presence of micromolar amounts of Cu2+ and Hg2+. It was also sensitive to oxidative inactivation by Fe2+ and H2O2 when both reagents were added in millimolar amounts. When oxidatively inactivated D-...

journal_title：Applied microbiology and biotechnology

authors： Schräder T,Andreesen JR

更新日期：1996-05-01 00:00:00

abstract：:Laccases are blue multicopper oxidases with potential applications in environmental and industrial biotechnology. In this study, a new bacterial laccase gene of 1.32 kb was obtained from a marine microbial metagenome of the South China Sea by using a sequence screening strategy. The protein (named as Lac15) of 439 ami...

journal_title：Applied microbiology and biotechnology

authors： Fang Z,Li T,Wang Q,Zhang X,Peng H,Fang W,Hong Y,Ge H,Xiao Y

更新日期：2011-02-01 00:00:00