Production of the polyketide 6-deoxyerythronolide B in the heterologous host Bacillus subtilis.

abstract：:A cDNA encoding a bifunctional acetylxylan esterase/xylanase, XynS20E, was cloned from the ruminal fungus Neocallimastix patriciarum. A putative conserved domain of carbohydrate esterase family 1 was observed at the N-terminus and a putative conserved domain of glycosyl hydrolase family 11 was detected at the C-termin...

journal_title：Applied microbiology and biotechnology

authors： Pai CK,Wu ZY,Chen MJ,Zeng YF,Chen JW,Duan CH,Li ML,Liu JR

更新日期：2010-02-01 00:00:00

abstract：:Engineering biosynthetic pathways in heterologous microbial host organisms offers an elegant approach to pathway elucidation via the incorporation of putative biosynthetic enzymes and characterization of resulting novel metabolites. Our previous work in Escherichia coli demonstrated the feasibility of a facile modular...

journal_title：Applied microbiology and biotechnology

authors： Morrone D,Lowry L,Determan MK,Hershey DM,Xu M,Peters RJ

更新日期：2010-02-01 00:00:00

abstract：:The recent use of photosynthetic organisms such as Chlamydomonas reinhardtii in biomedical applications has demonstrated their potential for the treatment of acute and chronic tissue hypoxia. Moreover, transgenic microalgae have been suggested as an alternative in situ drug delivery system. In this study, we set out t...

journal_title：Applied microbiology and biotechnology

authors： Jarquín-Cordero M,Chávez MN,Centeno-Cerdas C,Bohne AV,Hopfner U,Machens HG,Egaña JT,Nickelsen J

更新日期：2020-01-01 00:00:00

abstract：:Cytophaga hutchinsonii is a Gram-negative aerobic soil bacterium which can digest crystalline cellulose completely through a strategy different from that of the well-studied cellulolytic aerobic fungi and anaerobic bacteria. However, despite the availability of a published genome sequence, studies on this organism hav...

journal_title：Applied microbiology and biotechnology

authors： Xu Y,Ji X,Chen N,Li P,Liu W,Lu X

更新日期：2012-01-01 00:00:00

abstract：:In a screening for proteolytically active lactic acid bacteria, three strains, Lactobacillus delbrueckii ssp. lactis 92202, Lactobacillus helveticus 92201, and Lactobacillus delbrueckii ssp. bulgaricus 92059, showed the highest activities following growth in milk. All three strains degraded α- and β-casein, but did no...

journal_title：Applied microbiology and biotechnology

authors： Kliche T,Li B,Bockelmann W,Habermann D,Klempt M,de Vrese M,Wutkowski A,Clawin-Raedecker I,Heller KJ

更新日期：2017-10-01 00:00:00

abstract：:Transaminase enzymes (TAms) are becoming increasingly valuable in the chemist's toolbox as a biocatalytic route to chiral amines. Despite high profile successes, the lack of (R)-selective TAms and robustness under harsh industrial conditions continue to prove problematic. Herein, we report the isolation of the first h...

journal_title：Applied microbiology and biotechnology

authors： Kelly SA,Magill DJ,Megaw J,Skvortsov T,Allers T,McGrath JW,Allen CCR,Moody TS,Gilmore BF

更新日期：2019-07-01 00:00:00

abstract：:The carbon and nitrogen sources most suitable for L-asparaginase production by Enterobacter aerogenes were selected and their concentrations optimized in shake-flask cultures. Sodium citrate (1.0%) and diammonium hydrogen phosphate (0.16%) proved to be the best sources of carbon and nitrogen, respectively. Nitrogen ca...

journal_title：Applied microbiology and biotechnology

authors： Mukherjee J,Majumdar S,Scheper T

更新日期：2000-02-01 00:00:00

abstract：:Human epidermal growth factor receptors (EGFR) are an important target in drug discovery in terms of both protein-small-molecule interactions and protein-protein interactions. In this work, the isolation of a stable soluble protein of the tyrosine kinase domain of EGFR in Escherichia coli expression has been accomplis...

journal_title：Applied microbiology and biotechnology

authors： Seetaha S,Ratanabanyong S,Choowongkomon K

更新日期：2019-10-01 00:00:00

abstract：:Bacillus thuringiensis bacteria show insecticidal activities that rely upon the production of insecticidal crystal proteins, which are encoded by cry or cyt genes and can target a variety of insect pests. It has been shown that cry1Ac is the only cry gene in B. thuringiensis subsp. kurstaki HD73 (B. thuringiensis HD73...

journal_title：Applied microbiology and biotechnology

authors： Zhang X,Gao T,Peng Q,Song L,Zhang J,Chai Y,Sun D,Song F

更新日期：2018-04-01 00:00:00

abstract：:N-acetyl glutamate kinase (NAGK) is a key enzyme in the synthesis of L-arginine that is inhibited by its end product L-arginine in Corynebacterium glutamicum (C. glutamicum). In this study, the potential binding sites of arginine and the residues essential for its inhibition were identified by homology modeling, inhib...

journal_title：Applied microbiology and biotechnology

authors： Huang Y,Zhang H,Tian H,Li C,Han S,Lin Y,Zheng S

更新日期：2015-09-01 00:00:00

abstract：:Biocatalytic asymmetric preparation of (R)-1,3-butanediol has been attracting much attention in pharmaceuticals industry. A new ideal strain, ZJB-09162, which exhibited high reduction activity and excellent (R)-stereospecificity towards 4-hydroxy-2-butanone, has been successfully isolated from soil samples. Based on m...

journal_title：Applied microbiology and biotechnology

authors： Zheng RC,Ge Z,Qiu ZK,Wang YS,Zheng YG

更新日期：2012-05-01 00:00:00

abstract：:The Escherichia coli (E. coli) expression system has been widely used to produce recombinant proteins. However, in some heterologous expressions, there are still difficulties in large-scale production. The use of fusion partners is one of the strategies for improving the expression levels of proteins in E. coli host. ...

journal_title：Applied microbiology and biotechnology

authors： Nguyen TKM,Ki MR,Son RG,Pack SP

更新日期：2019-03-01 00:00:00

abstract：:A novel microbial transglutaminase (TGase) from the cultural filtrate of Streptomyces netropsis BCRC 12429 (Sn) was purified. The specific activity of the purified TGase was 18.2 U/mg protein with an estimated molecular mass of 38 kDa by sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis. The TGase gen...

journal_title：Applied microbiology and biotechnology

authors： Yu YJ,Wu SC,Chan HH,Chen YC,Chen ZY,Yang MT

更新日期：2008-12-01 00:00:00

abstract：:UV-mediated mutagenesis generated a high glucoamylase-producing mutant of Aspergillus oryzae exhibiting strong melanization in solid-state culture. Expression of the glucoamylase-encoding gene (glaB), which is specifically expressed in solid-state culture, and the tyrosinase-encoding gene (melO), was analyzed using an...

journal_title：Applied microbiology and biotechnology

authors： Ishida H,Matsumura K,Hata Y,Kawato A,Suginami K,Abe Y,Imayasu S,Ichishima E

更新日期：2001-10-01 00:00:00

abstract：:By using immobilized baker's yeast repeatedly in isooctane with occasional reactivation by cultivation, we succeeded in the preparation of an organic solvent-tolerant strain, named KK21, which could grow in the presence of isooctane. This is the first report on an organic solvent-tolerant strain from baker's yeast. St...

journal_title：Applied microbiology and biotechnology

authors： Kawamoto T,Kanda T,Tanaka A

更新日期：2001-05-01 00:00:00

abstract：:Gene amplification using dihydrofolate reductase gene (dhfr) and methotrexate (MTX) is widely used for recombinant protein production in mammalian cells and is typically conducted in DHFR-deficient Chinese hamster ovary (CHO) cell lines. Generation of DHFR-deficient cells can be achieved by an expression vector incorp...

journal_title：Applied microbiology and biotechnology

authors： Kang SY,Kim YG,Lee HW,Lee EG

更新日期：2015-12-01 00:00:00

abstract：:Biofilms in drinking water distribution systems (DWDSs) could cause several types of problems, such as the deterioration of water quality, corrosion of pipe walls, and potential proliferation of opportunistic pathogens. In this study, ten biofilm samples from different pipe materials, including ductile cast iron pipe ...

journal_title：Applied microbiology and biotechnology

authors： Ren H,Wang W,Liu Y,Liu S,Lou L,Cheng D,He X,Zhou X,Qiu S,Fu L,Liu J,Hu B

更新日期：2015-12-01 00:00:00

abstract：:Nervonic acid (NA) is a major very long-chain monounsaturated fatty acid found in the white matter of mammalian brains, which plays a critical role in the treatment of psychotic disorders and neurological development. In the nature, NA has been synthesized by a handful plants, fungi, and microalgae. Although the metab...

journal_title：Applied microbiology and biotechnology

authors： Fan Y,Meng HM,Hu GR,Li FL

更新日期：2018-04-01 00:00:00

abstract：:There is a worldwide trend to understand the impact of non-Saccharomyces yeast species on the process of winemaking. Although the predominant species at the end of the fermentation is Saccharomyces cerevisiae, several non-Saccharomyces species present during the first days of the process can produce and/or release aro...

journal_title：Applied microbiology and biotechnology

authors： Barquet M,Martín V,Medina K,Pérez G,Carrau F,Gaggero C

更新日期：2012-01-01 00:00:00

abstract：:The synthesis of rhamnosylated compounds has gained great importance since these compounds have potential therapeutic applications. The enzymatic approaches for glycosylation of bioactive molecules have been well developed; however, the enzymatic rhamnosylation has been largely hindered by lacking of the glycosyl dono...

journal_title：Applied microbiology and biotechnology

authors： Xu L,Liu X,Li Y,Yin Z,Jin L,Lu L,Qu J,Xiao M

更新日期：2019-10-01 00:00:00

abstract：:High poly(3-hydroxybutyrate) (PHB) content and volumetric productivity were achieved by fed-batch culture of Halomonas boliviensis using a defined medium. Initial shake flask cultivations in a minimal medium revealed that the growth of H. boliviensis was supported only when the medium was supplemented with aspartic ac...

journal_title：Applied microbiology and biotechnology

authors： Quillaguamán J,Doan-Van T,Guzmán H,Guzmán D,Martín J,Everest A,Hatti-Kaul R

更新日期：2008-02-01 00:00:00

abstract：:Understanding the effect of conserved domains reconstruction of alginate lyases on action mode is essential for their application and in-depth study. We report the expression and action mode of recombinant alginate lyase (AlyM) and its conserved domain reconstruction forms (AlyMΔCBM, cAlyM, and AlyMΔ58C). The enzymati...

journal_title：Applied microbiology and biotechnology

authors： Yang M,Li N,Yang S,Yu Y,Han Z,Li L,Mou H

更新日期：2019-01-01 00:00:00

abstract：:The operational temperature of microbial fuel cell reactors influences biofilm development, and this has an impact on anodic biocatalytic activity. In this study, we compared three microbial fuel cell (MFC) reactors acclimated at 10°C, 20°C and 35°C to investigate the effect on biomass development, methanogenesis and ...

journal_title：Applied microbiology and biotechnology

authors： Michie IS,Kim JR,Dinsdale RM,Guwy AJ,Premier GC

更新日期：2011-10-01 00:00:00

abstract：:Many hydrocarbon-contaminated environments are characterized by low or elevated temperatures, acidic or alkaline pH, high salt concentrations, or high pressure, Hydrocarbon-degrading microorganisms, adapted to grow and thrive in these environments, play an important role in the biological treatment of polluted extreme...

journal_title：Applied microbiology and biotechnology

authors： Margesin R,Schinner F

更新日期：2001-09-01 00:00:00

abstract：:Biosurfactant rhamnolipids have been claimed to show biological activities of inhibiting the proliferation of cancer cells. In this study, the cytotoxicity of rhamnolipids was examined on four cancer cells (HepG2, Caco-2, Hela, MCF-7 cells) and two normal cells (HK-2 cell, primary hepatocyte). Interestingly, both canc...

journal_title：Applied microbiology and biotechnology

authors： Jiang L,Shen C,Long X,Zhang G,Meng Q

更新日期：2014-12-01 00:00:00

abstract：:Regulatable promoters are important genetic tools, particularly for assigning function to essential and redundant genes. They can also be used to control the expression of enzymes that influence metabolic flux or protein secretion, thereby optimizing product yield in bioindustry. This review will focus on regulatable ...

journal_title：Applied microbiology and biotechnology

authors： Fuller KK,Dunlap JC,Loros JJ

更新日期：2018-05-01 00:00:00

abstract：:Biotechnological production of vanillin is gaining momentum as the natural synthesis of vanillin that is very expensive. Ferulic acid (FA), a costly compound, is used as the substrate to produce vanillin biotechnologically and the making process is still expensive. Therefore, this study investigated the practical use ...

journal_title：Applied microbiology and biotechnology

authors： Chakraborty D,Selvam A,Kaur B,Wong JWC,Karthikeyan OP

更新日期：2017-07-01 00:00:00

abstract：:Bacillus pumilus mutants were generated by targeted deletion of a set of genes eventually facilitating genetic handling and assuring biological containment. The well-defined and stable mutants do not form functional endospores due to the deletion of yqfD, an essential sporulation gene; they are affected in DNA repair,...

journal_title：Applied microbiology and biotechnology

authors： Wemhoff S,Meinhardt F

更新日期：2013-09-01 00:00:00

abstract：:Wastewater treatment plants (WWTPs) are major collection pools of antibiotics of which low concentrations may induce antibiotic resistance in their microbial communities and pose threat to human health. However, information is still limited on the microbial community alteration in WWTPs upon exposure to low-dose antib...

journal_title：Applied microbiology and biotechnology

authors： Wang S,Fan C,Low A,He J

更新日期：2014-03-01 00:00:00

abstract：:To improve the stability and catalytic efficiency of pyranose 2-oxidase (P2Ox) by molecular enzyme evolution, we cloned P2Ox cDNA by RACE-PCR from a cDNA library derived from the basidiomycete Peniophora gigantea. The P2Ox gene was expressed in Escherichia coli BL21(DE3), yielding an intracellular and enzymatically ac...

journal_title：Applied microbiology and biotechnology

authors： Bastian S,Rekowski MJ,Witte K,Heckmann-Pohl DM,Giffhorn F

更新日期：2005-06-01 00:00:00