Preparation of an organic solvent-tolerant strain from baker's yeast.

abstract：:After analysis of batch culture and identification of the ways for prolongation of citric acid active synthesis by yeast, repeat-batch (RB) cultivation was suggested. Yarrowia lipolytica strain RB cultivation was studied and optimal conditions for cultivation selected. It was shown that when applying RB cultivation, b...

journal_title：Applied microbiology and biotechnology

authors： Arzumanov TE,Shishkanova NV,Finogenova TV

更新日期：2000-05-01 00:00:00

abstract：:A comprehensive understanding of genotype-phenotype links in bacteria is the primary theme of bacterial functional genomics. Transposon sequencing (Tn-seq) or its equivalent methods that combine random transposon mutagenesis and next-generation sequencing (NGS) represent a powerful approach to understand gene function...

journal_title：Applied microbiology and biotechnology

authors： Kwon YM,Ricke SC,Mandal RK

更新日期：2016-01-01 00:00:00

abstract：:Vanillin can be produced on a commercial scale by depolymerising renewable lignin. One product of microbial metabolism of vanillin by common soil microbes, such as Acinetobacter baylyi, is a tricarboxylic acid with a butadiene backbone known as 3-carboxy muconate (3CM). Three enzymes, 4-hydroxy benzaldehyde dehydrogen...

journal_title：Applied microbiology and biotechnology

authors： Gosling A,Fowler SJ,O'Shea MS,Straffon M,Dumsday G,Zachariou M

更新日期：2011-04-01 00:00:00

abstract：:Biocatalytic asymmetric preparation of (R)-1,3-butanediol has been attracting much attention in pharmaceuticals industry. A new ideal strain, ZJB-09162, which exhibited high reduction activity and excellent (R)-stereospecificity towards 4-hydroxy-2-butanone, has been successfully isolated from soil samples. Based on m...

journal_title：Applied microbiology and biotechnology

authors： Zheng RC,Ge Z,Qiu ZK,Wang YS,Zheng YG

更新日期：2012-05-01 00:00:00

abstract：:Poly(ɛ-L-lysine) (ɛ-PL) is an unusual biopolymer composed of L-lysine connected between α-carboxyl and ɛ-amino groups. It has been used as a preservative in food and cosmetics industries, drug carrier in medicines, and gene carrier in gene therapy. Modern biotechnology has significantly improved the synthetic efficien...

journal_title：Applied microbiology and biotechnology

authors： Xu Z,Xu Z,Feng X,Xu D,Liang J,Xu H

更新日期：2016-08-01 00:00:00

abstract：:The objective of this study was to develop a non-virulent simulant to replace several virulent organisms during the development of detection and identification methods for biological threat agents. We identified and selected specific genes to detect Yersinia pestis, Francisella tularensis, Burkholderia mallei, Burkhol...

journal_title：Applied microbiology and biotechnology

authors： Carrera M,Sagripanti JL

更新日期：2009-01-01 00:00:00

abstract：:Antimicrobial dendrimeric peptides (AMDP) are a relatively new class of agents displaying repetitive functional groups on a branched core. Previously, we have investigated the length requirement for antimicrobial activity of peptides consisting of repeated arginine (R) and tryptophan (W) side chains and found that eve...

journal_title：Applied microbiology and biotechnology

authors： Bahar AA,Liu Z,Totsingan F,Buitrago C,Kallenbach N,Ren D

更新日期：2015-10-01 00:00:00

abstract：:Lactic acid bacteria are extensively used in food technology and for the production of various compounds, but they are fastidious in nutrient requirements. In order to elucidate the role of each component precisely, defined multicomponent media are required. This study focuses on determining nutrient auxotrophies and ...

journal_title：Applied microbiology and biotechnology

authors： Aller K,Adamberg K,Timarova V,Seiman A,Feštšenko D,Vilu R

更新日期：2014-07-01 00:00:00

abstract：:The production of infective juvenile stages (IJ) of the entomopathogenic nematode Steinernema carpocapsae in the presence of its symbiotic bacterium Xenorhabdus nematophilus was carried out in orbitally agitated bottles. Four complex culture media (M1-M4) were used, containing from 8% to 28% (by vol.) agave juice (agu...

journal_title：Applied microbiology and biotechnology

authors： Islas-López MA,Sanjuan-Galindo R,Rodríguez-Hernández AI,Chavarría-Hernández N

更新日期：2005-07-01 00:00:00

abstract：:A series of continuous- and sequencing-batch reactor experiments were performed to assess the growth dynamics of Escherichia coli strain K12-MG1655 in chemostat systems. Previous mathematical predictions and early experimental results had shown that confined oscillatory dynamics ensue in bioreactor populations, which ...

journal_title：Applied microbiology and biotechnology

authors： Ofiţeru ID,Ferdeş M,Knapp CW,Graham DW,Lavric V

更新日期：2012-04-01 00:00:00

abstract：:Phytoplankton blooms are predictable features of marine and freshwater habitats. Despite a good knowledge base of the environmental factors controlling blooms, complex interactions between the bacterial and archaeal communities and phytoplankton bloom taxa are only now emerging. Here, the current research on bacterial...

journal_title：Applied microbiology and biotechnology

authors： Song L

更新日期：2017-06-01 00:00:00

abstract：:Zearalenone (ZEN) is one of the common mycotoxins with quite high occurrence rate and is harmful to animal and human health. Lactobacillus reuteri is known as a probiotic bacterium with active immune stimulating and high inhibitory activity against pathogenic microorganisms. In this study, we expressed the lactonohydr...

journal_title：Applied microbiology and biotechnology

authors： Liu F,Malaphan W,Xing F,Yu B

更新日期：2019-11-01 00:00:00

abstract：:Bacillus cereus 905, one of the plant growth-promoting rhizobacteria (PGPRs), is capable of colonizing wheat roots in a large population size. From previous studies, we learned that the sodA2-encoding manganese-containing superoxide dismutase (MnSOD2) is important for B. cereus 905 to survive in wheat rhizosphere. In ...

journal_title：Applied microbiology and biotechnology

authors： Gao T,Ding M,Wang Q

更新日期：2020-11-01 00:00:00

abstract：:Immobilization of the Geotrichum candidum (CCT 1205) cell with functionalized silica creates promising biocatalysts for production of ɛ-caprolactone. The results obtained by immobilization of the whole cell on SiO2-NH2 and SiO2-SH supports indicate that the presence of reactive functional groups on the support may pro...

journal_title：Applied microbiology and biotechnology

authors： Silva ALP,da Silva Caridade TN,Magalhães RR,de Sousa KT,de Sousa CC,Vale JA

更新日期：2020-10-01 00:00:00

abstract：:Manipulation of product heterogeneity was attempted by using yeast extract as nitrogen source in Alternaria alternata S-f6 transformation process of 4'-demethylepipodophyllotoxin. When the nitrogen source of NaNO(3) was replaced by yeast extract, the heterogeneity of biotransformation products was significantly varied...

journal_title：Applied microbiology and biotechnology

authors： Zhao W,Li HM,Wan DJ,Tang YJ

更新日期：2012-01-01 00:00:00

abstract：:The gene encoding a putative triphenylmethane reductase (TMR)-like protein derived from Geobacillus thermoglucosidasius C56-Y593 (named as GtAZR) was synthesized, heterologously expressed in Escherichia coli, and extensively characterized for the first time. The recombinant GtAZR displayed its maximum activity at pH 5...

journal_title：Applied microbiology and biotechnology

authors： Gao F,Ding H,Shao L,Xu X,Zhao Y

更新日期：2015-01-01 00:00:00

abstract：:Non-covalent interaction of alcohol dehydrogenase with polysaccharides was studied using three neutral and three anionic polysaccharides. The process of interaction of alcohol dehydrogenase with gum Arabic was optimized with respect to the ratio of enzyme to gum Arabic, pH, and molarity of buffer. Alcohol dehydrogenas...

journal_title：Applied microbiology and biotechnology

authors： Jadhav SB,Bankar SB,Granström T,Ojamo H,Singhal RS,Survase SA

更新日期：2014-01-01 00:00:00

abstract：:In this work, a three-level Salmonella detection system based on a combination of seven SYBR®Green qPCR was developed. This detection system discriminates Salmonella at the genus, species and subspecies levels using a single 96-well plate. The SYBR®Green qPCR assays target the invA, rpoD, iroB and safC genes, as well ...

journal_title：Applied microbiology and biotechnology

authors： Barbau-Piednoir E,Bertrand S,Mahillon J,Roosens NH,Botteldoorn N

更新日期：2013-11-01 00:00:00

abstract：:The microbial communities of plant ecosystems are in relation to plant growing environment, but the alteration in biodiversity of rhizosphere and phyllosphere microbial communities in closed and controlled environments is unknown. The purpose of this study is to analyze the change regularity of microbial communities w...

journal_title：Applied microbiology and biotechnology

authors： Qin Y,Fu Y,Dong C,Jia N,Liu H

更新日期：2016-05-01 00:00:00

abstract：:Three different mechanisms can be envisaged that are used by fungi to solubilize coal: the production of alkaline substances, the extrusion of chelators and, of special interest in the scope of biotechnology, the action of enzymes. Whether these mechanisms are operating separately or in various combinations has not ye...

journal_title：Applied microbiology and biotechnology

authors： Holker U,Ludwig S,Scheel T,Hofer M

更新日期：1999-07-01 00:00:00

abstract：:The manganese superoxide dismutase gene (sodM) is very highly expressed in Aspergillus oryzae. To elucidate the basis for this high-level expression, deletion analysis of the promoter was undertaken using beta-glucuronidase (GUS) as a reporter. Deletion of a 63-bp sequence from -200 to -138 in the 1,038-bp sodM promot...

journal_title：Applied microbiology and biotechnology

authors： Hisada H,Sano M,Ishida H,Hata Y,Abe Y,Machida M

更新日期：2006-10-01 00:00:00

abstract：:In the Funding section, the following statement is missing: The MACS cohort study was supported by the NIH, National Institute of Allergy and Infectious Diseases grant U01-AI35041. ...

journal_title：Applied microbiology and biotechnology

authors： Palma ML,Garcia-Bates TM,Martins FS,Douradinha B

更新日期：2019-07-01 00:00:00

abstract：:Ralstonia eutropha H850 was labelled chromosomally with a gfp marker gene encoding for the green fluorescent protein, and designated R. eutropha H850g13. Visual observation of green fluorescent cells under an epifluorescence microscope, and PCR amplification products, confirmed that the bacterium was labelled with gfp...

journal_title：Applied microbiology and biotechnology

authors： Abbey AM,Beaudette LA,Lee H,Trevors JT

更新日期：2003-12-01 00:00:00

abstract：:The denitrifier community and associated nitrate and nitrite reduction in the bioaugmented and general sequencing batch reactors (SBRs) during the treatment of coking wastewater containing pyridine and quinoline were investigated. The efficiency and stability of nitrate and nitrite reduction in SBR was considerably im...

journal_title：Applied microbiology and biotechnology

authors： Bai Y,Sun Q,Xing R,Wen D,Tang X

更新日期：2011-05-01 00:00:00

abstract：:Thioredoxin is crucial for the maintenance of the redox status of cells of all types. Mammalian thioredoxin is secreted from various types of cells, although the mechanism underlying has not yet been clarified. Previously, we demonstrated that thioredoxin was released from Saccharomyces cerevisiae after treatment with...

journal_title：Applied microbiology and biotechnology

authors： Takeuchi Y,Nomura W,Ohdate T,Tamasu S,Masutani H,Murata K,Izawa S,Yodoi J,Inoue Y

更新日期：2007-07-01 00:00:00

abstract：:Recombinant production of mammalian cytoplasmic proteins plays a major role in developing pharmaceutical products. Here we describe two expression technologies using unique nature of halophilic bacteria. One of such properties of halophilic bacteria is accumulation of compatible solutes in the cytoplasm. As the compat...

journal_title：Applied microbiology and biotechnology

authors： Tokunaga H,Arakawa T,Tokunaga M

更新日期：2010-12-01 00:00:00

abstract：:Wild-type naphthalene dioxygenase (NDO) from Pseudomonas sp. strain NCIB 9816-4 transforms relatively planar flavone and isoflavone to cis-dihydrodiols. However, this enzyme cannot catalyze the transformation of flavanone and isoflavanone in which a phenyl group bonds to the stereogenic C2 or C3 of the C-ring. Protein...

journal_title：Applied microbiology and biotechnology

authors： Seo J,Ryu JY,Han J,Ahn JH,Sadowsky MJ,Hur HG,Chong Y

更新日期：2013-01-01 00:00:00

abstract：:Monascus pigments (Mps) are a group of azaphilonic secondary metabolites produced by Monascus spp. via a polyketide pathway. A mutant deleted an about 30 kb region of Mps gene cluster from Monascus ruber M7 was isolated previously, which produces a high amount of a light yellow pigment. The current study revealed that...

journal_title：Applied microbiology and biotechnology

authors： Liu J,Zhou Y,Yi T,Zhao M,Xie N,Lei M,Liu Q,Shao Y,Chen F

更新日期：2016-08-01 00:00:00

abstract：:The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a novel β-coronavirus, is the main pathogenic agent of the rapidly spreading pneumonia called coronavirus disease 2019 (COVID-19). SARS-CoV-2 infects much more people, especially the elder population, around the world than other coronavirus, such as SAR...

journal_title：Applied microbiology and biotechnology

authors： Chen X,Liao B,Cheng L,Peng X,Xu X,Li Y,Hu T,Li J,Zhou X,Ren B

更新日期：2020-09-01 00:00:00

abstract：:Fossil fuel reserves depletion, global warming, unrelenting population growth, and costly and problematic waste recycling call for renewable resources of energy and consumer products. As an alternative to the 100 % oil economy, production processes based on biomass can be developed. Huge amounts of lignocellulosic was...

journal_title：Applied microbiology and biotechnology

authors： Liguori R,Amore A,Faraco V

更新日期：2013-07-01 00:00:00