Novel soluble expression technologies derived from unique properties of halophilic proteins.

abstract：:It was found that benzothiazole, 2-oxybenzothiazole and 2-benzothiazolesulphonate were degraded in activated sludge systems. 2-Mercaptobenzothiazole (MBT) was more resistant, although the first step in MBT degradation seemed to be transformation to the sulphonate form. At higher MBT concentrations, it was transformed ...

journal_title：Applied microbiology and biotechnology

authors： De Vos D,De Wever H,Verachtert H

更新日期：1993-07-01 00:00:00

abstract：:Streptomyces lividans IAF18, obtained by homologous cloning, is capable of over-producing XlnA. To investigate the possibility of the expression of foreign genes, various coding regions of the xylanase A gene (xlnA) were analysed. Expression/secretion vectors were constructed containing the regulatory elements of xlnA...

journal_title：Applied microbiology and biotechnology

authors： Paradis FW,Shareck F,Dupont C,Kluepfel D,Morosoli R

更新日期：1996-06-01 00:00:00

abstract：:A cytochrome P450 and an iron-sulfur protein, whose expression was specifically induced during growth of Mycobacterium sp. strain HE5 on morpholine as the sole source of carbon, nitrogen, and energy were purified to apparent homogeneity. Due to the lack of enzymatic activity, carbon monoxide difference spectra and det...

journal_title：Applied microbiology and biotechnology

authors： Sielaff B,Andreesen JR,Schräder T

更新日期：2001-08-01 00:00:00

abstract：:The marine photosynthetic bacterium Rhodovulum sulfidophilum produces extracellular nucleic acids involved in its flocculation. Previously, we showed that the RNA fraction of these extracellular nucleic acids released into the culture medium contains mainly non-aminoacylated fully mature-sized tRNAs and fragments of 1...

journal_title：Applied microbiology and biotechnology

authors： Suzuki H,Daimon M,Awano T,Umekage S,Tanaka T,Kikuchi Y

更新日期：2009-08-01 00:00:00

abstract：:A widespread and hitherto by far underexploited potential among ecologically diverse fungi to pretreat wheat straw and digestate from maize silage in the future perspective of using such lignocellulosic feedstock for fermentative bioenergy production was inferred from a screening of nine freshwater ascomycetes, 76 iso...

journal_title：Applied microbiology and biotechnology

authors： Singh S,Harms H,Schlosser D

更新日期：2014-04-01 00:00:00

abstract：:In response to changing extracellular pH levels, phosphate-limited continuous cultures of Clostridium acetobutylicum reversibly switches its metabolism from the dominant formation of acids to the prevalent production of solvents. Previous experimental and theoretical studies have revealed that this pH-induced metaboli...

journal_title：Applied microbiology and biotechnology

authors： Millat T,Janssen H,Thorn GJ,King JR,Bahl H,Fischer RJ,Wolkenhauer O

更新日期：2013-07-01 00:00:00

abstract：:Escherichia coli-the powerhouse for recombinant protein production-is rapidly gaining status as a reliable and efficient host for secretory expression. An improved understanding of protein translocation processes and its mechanisms has inspired and accelerated the development of new tools and applications in this fiel...

journal_title：Applied microbiology and biotechnology

authors： Low KO,Muhammad Mahadi N,Md Illias R

更新日期：2013-05-01 00:00:00

abstract：:In order for microalgae-based processes to reach commercial production for biofuels and high-value products such as omega-3 fatty acids, it is necessary that economic feasibility be demonstrated at the industrial scale. Therefore, process optimization is critical to ensure that the maximum yield can be achieved from t...

journal_title：Applied microbiology and biotechnology

authors： Lowrey J,Armenta RE,Brooks MS

更新日期：2016-02-01 00:00:00

abstract：:A cDNA of a structural gene encoding pyranose 2-oxidase (P2O) from Trametes ochracea strain MB49 was cloned into Escherichia coli strain BL21(DE3) on a multicopy plasmid under the control of the trc promoter. Synthesis of P2O was studied in batch cultures in LB or M9-based mineral medium at 28 degrees C. While there w...

journal_title：Applied microbiology and biotechnology

authors： Vecerek B,Maresová H,Kocanová M,Kyslík P

更新日期：2004-05-01 00:00:00

abstract：:Lyme borreliosis is a bacterial infection that can be spread to humans by infected ticks and may severely affect many organs and tissues. Nearly four decades have elapsed since the discovery of the disease agent called Borrelia burgdorferi. Although there is a plethora of knowledge on the infectious agent and thousand...

journal_title：Applied microbiology and biotechnology

authors： Strnad M,Grubhoffer L,Rego ROM

更新日期：2020-03-01 00:00:00

abstract：:(-)-vibo-Quercitol is a deoxyinositol (1L-1,2,4/3,5-cyclohexanepentol) that occurs naturally in low concentrations in oak species, honeydew honey, and Gymnema sylvestre. The author's research group recently reported that (-)-vibo-quercitol and scyllo-quercitol (2-deoxy-myo-inositol, 1,3,5/2,4-cyclohexanepentol), a ste...

journal_title：Applied microbiology and biotechnology

authors： Itoh N

更新日期：2018-06-01 00:00:00

abstract：:Chemical mutagenesis of the phototrophic green microalga Parietochloris incisa, producing high amounts of arachidonic acid (ARA), resulted in selection of a mutant deficient in ARA and rich in dihomo-γ-linolenic acid (DGLA) and thus ∆5 desaturase defective. The mutagenesis produced a nonsense mutation in the ∆5 desatu...

journal_title：Applied microbiology and biotechnology

authors： Iskandarov U,Khozin-Goldberg I,Cohen Z

更新日期：2011-04-01 00:00:00

abstract：:Biocatalytic asymmetric preparation of (R)-1,3-butanediol has been attracting much attention in pharmaceuticals industry. A new ideal strain, ZJB-09162, which exhibited high reduction activity and excellent (R)-stereospecificity towards 4-hydroxy-2-butanone, has been successfully isolated from soil samples. Based on m...

journal_title：Applied microbiology and biotechnology

authors： Zheng RC,Ge Z,Qiu ZK,Wang YS,Zheng YG

更新日期：2012-05-01 00:00:00

abstract：:A halotolerant, alkaliphilic dissimilatory Fe(III)-reducing bacterium, strain SFB, was isolated from salt flat sediments collected from Soap Lake, WA. 16S ribosomal ribonucleic acid gene sequence analysis identified strain SFB as a novel Bacillus sp. most similar to Bacillus agaradhaerens (96.7% similarity). Strain SF...

journal_title：Applied microbiology and biotechnology

authors： Pollock J,Weber KA,Lack J,Achenbach LA,Mormile MR,Coates JD

更新日期：2007-12-01 00:00:00

abstract：:Nitrilase-mediated hydrolysis of isobutylsuccinonitrile (IBSN) is a highly attractive approach for (S)-3-cyano-5-methylhexanoic acid ((S)-CMHA), the critical chiral intermediate of pregabalin. In this study, a robust nitrilase from Arabis alpina (AaNIT) was screened and engineered. The N258D mutant was obtained with h...

journal_title：Applied microbiology and biotechnology

authors： Zhang Q,Wu ZM,Hao CL,Tang XL,Zheng RC,Zheng YG

更新日期：2019-07-01 00:00:00

abstract：:Although host proteases are often considered to have a negative impact upon heterologous protein production by filamentous fungi, relatively little is known about the pattern of their appearance in recombinant fungal bioprocesses. In the present study, we investigated extracellular proteases from a filamentous fungus,...

journal_title：Applied microbiology and biotechnology

authors： Li Q,Harvey LM,McNeil B

更新日期：2008-02-01 00:00:00

abstract：:A looming global energy crisis has directly increased biomethanation processes using anaerobic digestion technology. However, much knowledge on the microbial community structure, their distribution within the digester and related functions remains extremely scanty and unavailable in some cases, yet very valuable in th...

journal_title：Applied microbiology and biotechnology

authors： Ambuchi JJ,Liu J,Wang H,Shan L,Zhou X,Mohammed MO,Feng Y

更新日期：2016-05-01 00:00:00

abstract：:In this study, we aimed at determining the impact of naphthalene and different oxygen levels on a biofilm bacterial community originated from a petroleum hydrocarbon-contaminated groundwater. By using cultivation-dependent and cultivation-independent approaches, the enrichment, identification, and isolation of aerobic...

journal_title：Applied microbiology and biotechnology

authors： Benedek T,Szentgyörgyi F,Szabó I,Farkas M,Duran R,Kriszt B,Táncsics A

更新日期：2020-07-01 00:00:00

abstract：:Escherichia coli BL21, expressing a quintuple mutant of P450(BM-3), oxyfunctionalizes alpha-pinene in an NADPH-dependent reaction to alpha-pinene oxide, verbenol, and myrtenol. We optimized the whole-cell biocatalyst by integrating a recombinant intracellular NADPH regeneration system through co-expression of a glucos...

journal_title：Applied microbiology and biotechnology

authors： Schewe H,Kaup BA,Schrader J

更新日期：2008-02-01 00:00:00

abstract：:Tributyl phosphate (TBP) is a toxic organophosphorous compound widely used in nuclear fuel processing and chemical industries. Rhodopseudomonas palustris, one of the most metabolically versatile photosynthetic bacteria, is shown here to degrade TBP efficiently under photosynthetic conditions. This study shows that thi...

journal_title：Applied microbiology and biotechnology

authors： Berne C,Pignol D,Lavergne J,Garcia D

更新日期：2007-11-01 00:00:00

abstract：:Transformants of the Trichoderma reeseistrains QM9414 and Rut-C30 were constructed in which the genes for the two major hydrophobin proteins, hydrophobins I (HFBI) and II (HFBII), were deleted or amplified by molecular biological techniques. Growth parameters and foam production of the transformant strains were compar...

journal_title：Applied microbiology and biotechnology

authors： Bailey MJ,Askolin S,Hörhammer N,Tenkanen M,Linder M,Penttilä M,Nakari-Setälä T

更新日期：2002-05-01 00:00:00

abstract：:Leavening ability in sweet dough is required for the commercial applications of baker's yeast. This property depends on many factors, such as glycolytic activity, sucrase activity, and osmotolerance. This study explored the importance of sucrase level on the leavening ability of baker's yeast in sweet dough. Furthermo...

journal_title：Applied microbiology and biotechnology

authors： Zhang CY,Lin X,Feng B,Liu XE,Bai XW,Xu J,Pi L,Xiao DG

更新日期：2016-07-01 00:00:00

abstract：:Ginsenosides are believed to be the principal components behind the pharmacological actions of ginseng, and their bioactive properties are closely related to the type, position, and number of sugar moieties attached to the aglycone; thus, modification of the sugar chains may markedly change their biological activities...

journal_title：Applied microbiology and biotechnology

authors： Tan JS,Yeo CR,Popovich DG

更新日期：2017-07-01 00:00:00

abstract：:We are beginning to see how the microbiota of the human gastrointestinal tract (GIT) can drive the development of new products to benefit human health and wellbeing. Despite the growing market for prebiotics and probiotics, there are currently no commercial products available that aid or increase the attachment of hea...

journal_title：Applied microbiology and biotechnology

authors： Morrin ST,Hickey RM

更新日期：2020-02-01 00:00:00

abstract：:Acetic acid bacteria are well-known for their membrane-bound dehydrogenases rapidly oxidizing a variety of substrates in the periplasm. Since many acetic acid bacteria have not been successfully cultured in the laboratory yet, studying membrane-bound dehydrogenases directly from a metagenome of vinegar microbiota seem...

journal_title：Applied microbiology and biotechnology

authors： Peters B,Mientus M,Kostner D,Daniel R,Liebl W,Ehrenreich A

更新日期：2017-11-01 00:00:00

abstract：:Various techniques are used to adjust the flavors of foods and beverages to new market demands. Although synthetic flavoring chemicals are still widely used, flavors produced by biological methods (bioflavors) are now more and more requested by consumers, increasingly concerned with health and environmental problems c...

journal_title：Applied microbiology and biotechnology

authors： Vanderhaegen B,Neven H,Coghe S,Verstrepen KJ,Derdelinckx G,Verachtert H

更新日期：2003-08-01 00:00:00

abstract：:The β-fructofuranosidase Xd-INV from the yeast Xanthophyllomyces dendrorhous is the largest microbial enzyme producing neo-fructooligosaccharides (neo-FOS) known to date. It mainly synthesizes neokestose and neonystose, oligosaccharides with potentially improved prebiotic properties. The Xd-INV gene comprises an open ...

journal_title：Applied microbiology and biotechnology

authors： Gimeno-Pérez M,Linde D,Fernández-Arrojo L,Plou FJ,Fernández-Lobato M

更新日期：2015-04-01 00:00:00

abstract：:Entomopathogenic fungi are widely produced for use as mycoinsecticides. Therefore, improvement of the shelf life of fungal propagules under good and adverse conditions should be a pre-requisite of their production. In order to improve conidial physiology as well as mycoinsecticide efficiency, culture conditions may be...

journal_title：Applied microbiology and biotechnology

authors： Tarocco F,Lecuona RE,Couto AS,Arcas JA

更新日期：2005-09-01 00:00:00

abstract：:Guanosine 5'-triphosphate (GTP) is the key substrate for biosynthesis of guanosine 5'-diphosphate (GDP)-L-fucose. In this study, improvement of GDP-L-fucose production was attempted by manipulating the biosynthetic pathway for guanosine nucleotides in recombinant Escherichia coli-producing GDP-L-fucose. The effects of...

journal_title：Applied microbiology and biotechnology

authors： Lee WH,Shin SY,Kim MD,Han NS,Seo JH

更新日期：2012-03-01 00:00:00

abstract：:This study was undertaken to assess the antifungal performance of three different Lactobacillus species.Experiments were conducted in vitro and in situ to extend the shelf life of wheat bread. Standard sourdough analyses were performed characterising acidity and carbohydrate levels. Overall, the strains showed good in...

journal_title：Applied microbiology and biotechnology

authors： Axel C,Brosnan B,Zannini E,Peyer LC,Furey A,Coffey A,Arendt EK

更新日期：2016-02-01 00:00:00