Modulation of guanosine nucleotides biosynthetic pathways enhanced GDP-L-fucose production in recombinant Escherichia coli.

abstract：:A phylogeny-guided approach was applied to screen endophytic fungi containing type I polyketide synthase (PKS I) biosynthetic gene sequences and aimed to correlate genotype to chemotype for the discovery of novel bioactive polyketides. Salicorn 58, which was identified as Talaromyces funiculosus based on its internal ...

journal_title：Applied microbiology and biotechnology

authors： Guo J,Ran H,Zeng J,Liu D,Xin Z

更新日期：2016-06-01 00:00:00

abstract：:In this study, the kinetic behaviors between n-butyl acetate and composite bead were investigated. Both microbial growth rate and biochemical reaction rate would be inhibited with increasing average inlet concentration. The order of the inhibitive effect, which resulted from increased average inlet concentration for f...

journal_title：Applied microbiology and biotechnology

authors： Chan WC,Hwang ID

更新日期：2006-11-01 00:00:00

abstract：:Two extracellular enzymes (MsP1 and MsP2) capable of efficient beta-carotene degradation were purified from culture supernatants of the basidiomycete Marasmius scorodonius (garlic mushroom). Under native conditions, the enzymes exhibited molecular masses of approximately 150 and approximately 120 kDa, respectively. SD...

journal_title：Applied microbiology and biotechnology

authors： Scheibner M,Hülsdau B,Zelena K,Nimtz M,de Boer L,Berger RG,Zorn H

更新日期：2008-01-01 00:00:00

abstract：:Atherosclerosis is a major cause of mortalities and morbidities worldwide. It is associated with hyperlipidemia and inflammation, and become chronic by triggering metabolites in different metabolic pathways. Disturbance in the human gut microbiota is now considered a critical factor in the atherosclerosis. Trimethylam...

journal_title：Applied microbiology and biotechnology

authors： Din AU,Hassan A,Zhu Y,Yin T,Gregersen H,Wang G

更新日期：2019-12-01 00:00:00

abstract：:This review describes the historical development and current state of metals leaching and sulfide mineral biooxidation by the minerals industries. During the past 20 years commercial processes employing microorganisms for mineral recovery have progressed from rather uncontrolled copper dump leaching to mineral oxidati...

journal_title：Applied microbiology and biotechnology

authors： Olson GJ,Brierley JA,Brierley CL

更新日期：2003-12-01 00:00:00

abstract：:Over 9,000 flavonoid compounds have been found in various plants, comprising one of the largest families of natural products. Flavonoids are an essential factor in plant interactions with the environment, often serving as the first line of defense against UV irradiation and pathogen attacks. Flavonoids are also major ...

journal_title：Applied microbiology and biotechnology

authors： Wang Y,Chen S,Yu O

更新日期：2011-08-01 00:00:00

abstract：:Nanosized elemental sulfur (ES) is already reported to exert superior antimicrobial efficacy than micron-sized ES, which encourages their use in drugs and therapeutics. The aim of the present study is to explore the possible route and mode of antimicrobial action of orthorhombic (α-SNPs) and monoclinic (β-SNPs) allotr...

journal_title：Applied microbiology and biotechnology

authors： Roy Choudhury S,Mandal A,Ghosh M,Basu S,Chakravorty D,Goswami A

更新日期：2013-07-01 00:00:00

abstract：:The essential general features required for lipid membranes of extremophilic archaea to fulfill biological functions are that they are in the liquid crystalline phase and have extremely low permeability of solutes that is much less temperature sensitive due to a lack of lipid-phase transition and highly branched isopr...

journal_title：Applied microbiology and biotechnology

authors： Ulrih NP,Gmajner D,Raspor P

更新日期：2009-08-01 00:00:00

abstract：:A cyclodextrin glucanotransferase (CGTase) gene of Bacillus ohbensis was cloned in Escherichia coli and the nucleotide sequence was determined. A single open reading frame (2112 bp) with a TTG codon as an initiator was identified that encodes a typical signal peptide of 29 amino acids followed by the mature enzyme (67...

journal_title：Applied microbiology and biotechnology

authors： Sin K,Nakamura A,Kobayashi K,Masaki H,Uozumi T

更新日期：1991-08-01 00:00:00

abstract：:The Ser-170 residue of a trypsin-like enzyme from Streptomyces fradiae (SFT), which is considered to be the active-site serine, was replaced with alanine by site-directed mutagenesis to improve the affinity chromatography step for a Kazal-type trypsin inhibitor pancreatic secretory trypsin inhibitor (PSTI). The result...

journal_title：Applied microbiology and biotechnology

authors： Katoh T,Kikuchi N,Nagata K,Yoshida N

更新日期：1996-08-01 00:00:00

abstract：:Cytophaga hutchinsonii is a Gram-negative aerobic soil bacterium which can digest crystalline cellulose completely through a strategy different from that of the well-studied cellulolytic aerobic fungi and anaerobic bacteria. However, despite the availability of a published genome sequence, studies on this organism hav...

journal_title：Applied microbiology and biotechnology

authors： Xu Y,Ji X,Chen N,Li P,Liu W,Lu X

更新日期：2012-01-01 00:00:00

abstract：:Simple phenolic components of wine, hydroxycinnamic acids (HCAs) are known to have antimicrobial properties. This study sought to determine the potential of ferulic acid as an antifungal agent for the control of Dekkera. Growth was inhibited by all HCAs examined in this study, with ferulic acid being the most potent a...

journal_title：Applied microbiology and biotechnology

authors： Harris V,Jiranek V,Ford CM,Grbin PR

更新日期：2010-03-01 00:00:00

abstract：:Proteins that catalyze H2-pathways often contain iron-sulfur (Fe-S) clusters and are sensitive to O2. We tested whether deletion of the gene encoding the transcriptional negative regulator, IscR, could enhance the ability of Escherichia coli BL21 to synthesize active recombinant H2-pathway components and stimulate fer...

journal_title：Applied microbiology and biotechnology

authors： Akhtar MK,Jones PR

更新日期：2008-04-01 00:00:00

abstract：:Uniform stable-isotope labeling of mammalian cells is achieved via a novel formulation of a serum-free cell culture medium that is based on stable-isotope-labeled autolysates and lipid extracts of various microbiological origin. Yeast autolysates allow complete replacement of individual amino acids and organic acids i...

journal_title：Applied microbiology and biotechnology

authors： Egorova-Zachernyuk TA,Bosman GJ,Degrip WJ

更新日期：2011-01-01 00:00:00

abstract：:This paper studies the initial stages of iridescent tarnishes on titanium heat exchanger tubes in contact with running freshwater on the river Tagus in Spain for up to 20 months. Electrochemical impedance spectroscopy (EIS), scanning electron microscopy [(SEM with energy dispersive X-ray (EDX)] and X-ray photoelectron...

journal_title：Applied microbiology and biotechnology

authors： Moreno DA,Cano E,Ibars JR,Polo JL,Montero F,Bastidas JM

更新日期：2004-05-01 00:00:00

abstract：:Acetic acid bacteria are well-known for their membrane-bound dehydrogenases rapidly oxidizing a variety of substrates in the periplasm. Since many acetic acid bacteria have not been successfully cultured in the laboratory yet, studying membrane-bound dehydrogenases directly from a metagenome of vinegar microbiota seem...

journal_title：Applied microbiology and biotechnology

authors： Peters B,Mientus M,Kostner D,Daniel R,Liebl W,Ehrenreich A

更新日期：2017-11-01 00:00:00

abstract：:Confident protein-DNA interaction (PDI) data could significantly improve our understanding of transcriptional regulation network in both prokaryotes and eukaryotes. New methods need to be established for validating and mining the protein-DNA interaction data produced by bioinformatic tools and large-scale screening as...

journal_title：Applied microbiology and biotechnology

authors： Zeng J,Li Y,Zhang S,He ZG

更新日期：2012-02-01 00:00:00

abstract：:Increasing evidence has revealed a close association between intestinal bacterial communities and human health. However, given that host phylogeny shapes the composition of intestinal microbiota, it is unclear whether changes in intestinal microbiota structure in relation to shrimp health status. In this study, we col...

journal_title：Applied microbiology and biotechnology

authors： Xiong J,Wang K,Wu J,Qiuqian L,Yang K,Qian Y,Zhang D

更新日期：2015-08-01 00:00:00

abstract：:Autohydrogenotrophic batch growth of Ralstonia eutropha H16 was studied in a stirred-tank reactor with nitrate and nitrite as terminal electron acceptors and the sole limiting substrates. Assuming product inhibition by nitrite, saturation kinetics with the two limiting substrates and a simple switching function, which...

journal_title：Applied microbiology and biotechnology

authors： Tiemeyer A,Link H,Weuster-Botz D

更新日期：2007-08-01 00:00:00

abstract：:The ability of taxonomically different yeasts to synthesize pyruvic acid (PA) from glucose was studied. The study showed that many yeasts are able to produce PA from glucose under the condition of growth limitation by thiamine. This ability was found in the yeast Blastobotrys adeninivorans for the first time. The prod...

journal_title：Applied microbiology and biotechnology

authors： Kamzolova SV,Morgunov IG

更新日期：2016-09-01 00:00:00

abstract：:The influence of different amino acid substitutions in the nitrilase from Pseudomonas fluorescens EBC191 (NitA) on the catalytical activity and the ability to form amides was investigated. The enzyme variant Glu137Ala was constructed because glutamate residues homologous to Glu137 are highly conserved among different ...

journal_title：Applied microbiology and biotechnology

authors： Sosedov O,Stolz A

更新日期：2015-03-01 00:00:00

abstract：:2-Hydroxyalkanoates (2HAs) have become the new monomeric constituents of bacterial polyhydroxyalkanoates (PHAs). PHAs containing 2HA monomers, lactate (LA), glycolate (GL), and 2-hydroxybutyrate (2HB) can be synthesized by engineered microbes in which the broad substrate specificities of PHA synthase and propionyl-CoA...

journal_title：Applied microbiology and biotechnology

authors： Matsumoto K,Taguchi S

更新日期：2013-09-01 00:00:00

abstract：:Grapevines are sensitive to a wide range of fungal pathogens, including agents such as Phaeomoniella chlamydospora and Phaeoacremonium aleophilum that cause tracheomycosis. In the present study, a procedure for DNA extraction from grapevine woody tissue is first evaluated and shown to be suitable for quantitative anal...

journal_title：Applied microbiology and biotechnology

authors： Pouzoulet J,Mailhac N,Couderc C,Besson X,Daydé J,Lummerzheim M,Jacques A

更新日期：2013-12-01 00:00:00

abstract：:Diastereoselective reduction of diketones with Lactobacillus kefir DSM 20587 was examined. The reduction of both oxo-functions proceeded highly diastereoselectively. (2 R,5 R)-Hexanediol 3 was produced starting from (2,5)-hexanedione 1 in quantitative yields with enantiomeric excess >99% and diastereomeric excess >99%...

journal_title：Applied microbiology and biotechnology

authors： Haberland J,Kriegesmann A,Wolfram E,Hummel W,Liese A

更新日期：2002-04-01 00:00:00

abstract：:Bioconversion of lignocellulosic biomass to high-value bioproducts by fermentative microorganisms has drawn extensive attentions worldwide. Lignocellulosic biomass cannot be efficiently utilized by microorganisms, such as Saccharomyces cerevisiae, but has to be pretreated prior to fermentation. Aldehyde compounds, as ...

journal_title：Applied microbiology and biotechnology

authors： Wang H,Li Q,Kuang X,Xiao D,Han X,Hu X,Li X,Ma M

更新日期：2018-12-01 00:00:00

abstract：:Variable domains of heavy chains of camelid heavy-chain antibodies (VHHs) are known as nanobodies. Nanobodies are approximately 15 kDa in size with high affinity to their antigens. They can be easily manipulated and produced in microorganisms. In this study, an alpaca was immunized with purified green fluorescence pro...

journal_title：Applied microbiology and biotechnology

authors： Fang Z,Cao D,Qiu J

更新日期：2020-06-01 00:00:00

abstract：:The binaphthyl derivative cladosporol 3 was supplied from 60 to 200 mg l(-1) to shaken cultures of Cladosporium cf. cladosporioides. Compared to blank, fungal biomass was not affected by adding cladosporol till 100 mg l(-1): it rather increased at higher ratios between 150 and 200 mg l(-1). The production of the major...

journal_title：Applied microbiology and biotechnology

authors： Assante G,Bava A,Nasini G

更新日期：2006-02-01 00:00:00

abstract：:The thioredoxin system plays a critical role in maintaining the cytoplasm redox state, participating in functions that are important to the cellular viability of fungi. Although functional and structural information on targets in human pathogenic fungi has been scarcely described in the literature, such studies are es...

journal_title：Applied microbiology and biotechnology

authors： Godoy JS,Kioshima ÉS,Abadio AK,Felipe MS,de Freitas SM,Svidzinski TI

更新日期：2016-05-01 00:00:00

abstract：:In this study, we employed TiO₂ enrichment and high accuracy liquid chromatography-mass spectrometry-mass spectrometry to identify the phosphoproteome of Clostridium acetobutyicum ATCC824 in acidogenesis and solventogenesis. As many as 82 phosphopeptides in 61 proteins, with 107 phosphorylated sites on serine, threoni...

journal_title：Applied microbiology and biotechnology

authors： Bai X,Ji Z

更新日期：2012-07-01 00:00:00

abstract：:Oleaginous yeasts (18 strains) were grown in ethanol media under various cultivation conditions (33 biomass samples). It was found that lipid and lipid-free fractions of dry biomass have elemental composition and biomass reductivity very close to values which can be considered as biological constants. The energy conte...

journal_title：Applied microbiology and biotechnology

authors： Minkevich IG,Dedyukhina EG,Chistyakova TI

更新日期：2010-10-01 00:00:00